Strain identifier

BacDive ID: 23295

Type strain: No

Species: Staphylococcus aureus

Strain Designation: MQCL 113

Strain history: CIP <- 2014, NCTC <- 2003, P. Bracegirdle, Public Health, Colindale, London, UK: strain MQCL 113

NCBI tax ID(s): 1280 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 18923

BacDive-ID: 23295

DSM-Number: 27146

keywords: Bacteria, aerobe, mesophilic, Gram-positive, coccus-shaped, antibiotic resistance, human pathogen

description: Staphylococcus aureus MQCL 113 is an aerobe, mesophilic, Gram-positive human pathogen that has multiple antibiotic resistances.

NCBI tax id

  • NCBI tax id: 1280
  • Matching level: species

strain history

@refhistory
18923<- P. Bracegirdle, Health Protection Agency Culture Collections, Salisbury, United Kingdom; NCTC 12493 <- Public Health Laboratory Services, Colindale, London, United Kingdom
116616CIP <- 2014, NCTC <- 2003, P. Bracegirdle, Public Health, Colindale, London, UK: strain MQCL 113

doi: 10.13145/bacdive23295.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Staphylococcaceae
  • genus: Staphylococcus
  • species: Staphylococcus aureus
  • full scientific name: Staphylococcus aureus Rosenbach 1884 (Approved Lists 1980)
  • synonyms

    • @ref: 20215
    • synonym: Staphylococcus aureus subsp. anaerobius

@ref: 18923

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Staphylococcaceae

genus: Staphylococcus

species: Staphylococcus aureus

full scientific name: Staphylococcus aureus Rosenbach 1884

strain designation: MQCL 113

type strain: no

Morphology

cell morphology

  • @ref: 116616
  • gram stain: positive
  • cell shape: coccus-shaped
  • motility: no

colony morphology

  • @ref: 18923
  • type of hemolysis: beta
  • hemolysis ability: 1
  • incubation period: 1-2 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
18923COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
18923TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
41602MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
116616CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
18923positivegrowth37mesophilic
41602positivegrowth37mesophilic
63395positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 63395
  • oxygen tolerance: aerobe

antibiogram

@refmediumincubation temperatureincubation timeoxygen conditionPenicillin Goxacillinampicillinticarcillinmezlocillincefalotincefazolincefotaximeaztreonamimipenemtetracyclinechloramphenicolgentamycinamikacinvancomycinerythromycinlincomycinofloxacinnorfloxacincolistinpipemidic acidnitrofurantoinbacitracinpolymyxin bkanamycinneomycindoxycyclineceftriaxoneclindamycinfosfomycinmoxifloxacinlinezolidnystatinquinupristin/dalfopristinteicoplaninpiperacillin/tazobactam
18923Mueller-Hinton Agar371aerob1226142216-183228220406262020200026-28240102416-18820181220036-3834340302024
18923Mueller-Hinton Agar371aerob1226162216322822042628-30202020003022-240824186201812228-1034-3632-34360302024
18923Mueller-Hinton Agar37aerob1426-281620-2218322822040828-30222220-22003024082618822201620034-363438-40030-3218-2026

observation

  • @ref: 18923
  • observation: not MRSA

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6837518257ornithine-degradation
683754853esculin-hydrolysis
6837517634D-glucose+fermentation
6837515824D-fructose+fermentation
6837516024D-mannose+fermentation
6837517306maltose+fermentation
6837517716lactose+fermentation
6837527082trehalose+fermentation
6837516899D-mannitol+fermentation
6837516634raffinose-fermentation
6837516988D-ribose-fermentation
6837517057cellobiose-fermentation
6837517632nitrate+reduction
6837517992sucrose+fermentation
6837559640N-acetylglucosamine+fermentation
6837532528turanose+fermentation
6837522599arabinose-fermentation

enzymes

@refvalueactivityec
18923catalase+1.11.1.6
18923cytochrome-c oxidase-1.9.3.1
68375beta-glucuronidase-3.2.1.31
68375alkaline phosphatase+3.1.3.1
68375L-arginine arylamidase-
68375beta-galactosidase-3.2.1.23
68375beta-glucosidase-3.2.1.21
68375ornithine decarboxylase-4.1.1.17

API ID32STA

@refUREADH ArgODCESCGLUFRUMNEMALLACTREMANRAFRIBCELNITVPbeta GALArgAPALPyrANOVOSACNAGTURARAbeta GUR
18923++--+++++++---++--++-+++--
18923+---+++++++---++--+-++++--
18923+---+++++++---++--+--+++--
18923-+--+++++++---++--+-+/-+++--
63395-+--+++++++---+---+--+++--

Isolation, sampling and environmental information

isolation

@refcountryorigin.countrycontinent
18923United KingdomGBREurope
63395United KingdomGBREurope
116616United KingdomGBREurope

Safety information

risk assessment

@refpathogenicity humanpathogenicity animalbiosafety levelbiosafety level comment
18923yesyes2Risk group (German classification)
1166162Risk group (French classification)

External links

@ref: 18923

culture collection no.: DSM 27146, NCTC 12493, WDCM 00212, CCUG 67181, CIP 110856, CECT 9152

literature

topicPubmed-IDtitleauthorsjournalDOIyearmesh
Pathogenicity16787954Reversing methicillin resistance in MRSA using a bacterial transforming agent.Carey BE, Dancer SJJ Antimicrob Chemother10.1093/jac/dkl2452006Anti-Bacterial Agents/*pharmacology, Drug Interactions, Methicillin/*pharmacology, Methicillin Resistance/*drug effects, Microbial Sensitivity Tests, Staphylococcus aureus/*drug effects
Pathogenicity21263047Resistance and tolerance to tropodithietic acid, an antimicrobial in aquaculture, is hard to select.Porsby CH, Webber MA, Nielsen KF, Piddock LJ, Gram LAntimicrob Agents Chemother10.1128/AAC.01222-102011Anti-Bacterial Agents/*pharmacology, Drug Resistance, Bacterial, Magnetic Resonance Spectroscopy, Microbial Sensitivity Tests, Roseobacter/drug effects, Salmonella enterica/drug effects, Staphylococcus aureus/drug effects, Tropolone/*analogs & derivatives/pharmacology
Phylogeny33597879Antibacterial Activities of Echinops kebericho Mesfin Tuber Extracts and Isolation of the Most Active Compound, Dehydrocostus Lactone.Deyno S, Mtewa AG, Hope D, Bazira J, Makonnen E, Alele PEFront Pharmacol10.3389/fphar.2020.6086722021
34588904Antibacterial potential of Malaysian ethnomedicinal plants against methicillin-susceptible Staphylococcus aureus (MSSA) and methicillin-resistant Staphylococcus aureus (MRSA).Yi Xin L, Hui Min T, Nur Liyana Mohd Zin P, Pulingam T, Nelson Appaturi J, Parumasivam TSaudi J Biol Sci10.1016/j.sjbs.2021.06.0362021

Reference

@idauthorscataloguedoi/urltitle
18923Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 27146)https://www.dsmz.de/collection/catalogue/details/culture/DSM-27146
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
41602Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/34410
63395Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 67181)https://www.ccug.se/strain?id=67181
68375Automatically annotated from API ID32STA
116616Curators of the CIPCollection of Institut Pasteur (CIP 110856)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20110856