Strain identifier
BacDive ID: 23295
Type strain:
Species: Staphylococcus aureus
Strain Designation: MQCL 113
Strain history: CIP <- 2014, NCTC <- 2003, P. Bracegirdle, Public Health, Colindale, London, UK: strain MQCL 113
NCBI tax ID(s): 1280 (species)
General
@ref: 18923
BacDive-ID: 23295
DSM-Number: 27146
keywords: Bacteria, aerobe, mesophilic, Gram-positive, coccus-shaped, antibiotic resistance, human pathogen
description: Staphylococcus aureus MQCL 113 is an aerobe, mesophilic, Gram-positive human pathogen that has multiple antibiotic resistances.
NCBI tax id
- NCBI tax id: 1280
- Matching level: species
strain history
@ref | history |
---|---|
18923 | <- P. Bracegirdle, Health Protection Agency Culture Collections, Salisbury, United Kingdom; NCTC 12493 <- Public Health Laboratory Services, Colindale, London, United Kingdom |
116616 | CIP <- 2014, NCTC <- 2003, P. Bracegirdle, Public Health, Colindale, London, UK: strain MQCL 113 |
doi: 10.13145/bacdive23295.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Bacilli
- order: Caryophanales
- family: Staphylococcaceae
- genus: Staphylococcus
- species: Staphylococcus aureus
- full scientific name: Staphylococcus aureus Rosenbach 1884 (Approved Lists 1980)
synonyms
- @ref: 20215
- synonym: Staphylococcus aureus subsp. anaerobius
@ref: 18923
domain: Bacteria
phylum: Firmicutes
class: Bacilli
order: Bacillales
family: Staphylococcaceae
genus: Staphylococcus
species: Staphylococcus aureus
full scientific name: Staphylococcus aureus Rosenbach 1884
strain designation: MQCL 113
type strain: no
Morphology
cell morphology
- @ref: 116616
- gram stain: positive
- cell shape: coccus-shaped
- motility: no
colony morphology
- @ref: 18923
- type of hemolysis: beta
- hemolysis ability: 1
- incubation period: 1-2 days
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
18923 | COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) | yes | https://mediadive.dsmz.de/medium/693 | Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base |
18923 | TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) | yes | https://mediadive.dsmz.de/medium/92 | Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water |
41602 | MEDIUM 72- for trypto casein soja agar | yes | Distilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g) | |
116616 | CIP Medium 72 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
18923 | positive | growth | 37 | mesophilic |
41602 | positive | growth | 37 | mesophilic |
63395 | positive | growth | 37 | mesophilic |
Physiology and metabolism
oxygen tolerance
- @ref: 63395
- oxygen tolerance: aerobe
antibiogram
@ref | medium | incubation temperature | incubation time | oxygen condition | Penicillin G | oxacillin | ampicillin | ticarcillin | mezlocillin | cefalotin | cefazolin | cefotaxime | aztreonam | imipenem | tetracycline | chloramphenicol | gentamycin | amikacin | vancomycin | erythromycin | lincomycin | ofloxacin | norfloxacin | colistin | pipemidic acid | nitrofurantoin | bacitracin | polymyxin b | kanamycin | neomycin | doxycycline | ceftriaxone | clindamycin | fosfomycin | moxifloxacin | linezolid | nystatin | quinupristin/dalfopristin | teicoplanin | piperacillin/tazobactam |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
18923 | Mueller-Hinton Agar | 37 | 1 | aerob | 12 | 26 | 14 | 22 | 16-18 | 32 | 28 | 22 | 0 | 40 | 6 | 26 | 20 | 20 | 20 | 0 | 0 | 26-28 | 24 | 0 | 10 | 24 | 16-18 | 8 | 20 | 18 | 12 | 20 | 0 | 36-38 | 34 | 34 | 0 | 30 | 20 | 24 |
18923 | Mueller-Hinton Agar | 37 | 1 | aerob | 12 | 26 | 16 | 22 | 16 | 32 | 28 | 22 | 0 | 42 | 6 | 28-30 | 20 | 20 | 20 | 0 | 0 | 30 | 22-24 | 0 | 8 | 24 | 18 | 6 | 20 | 18 | 12 | 22 | 8-10 | 34-36 | 32-34 | 36 | 0 | 30 | 20 | 24 |
18923 | Mueller-Hinton Agar | 37 | aerob | 14 | 26-28 | 16 | 20-22 | 18 | 32 | 28 | 22 | 0 | 40 | 8 | 28-30 | 22 | 22 | 20-22 | 0 | 0 | 30 | 24 | 0 | 8 | 26 | 18 | 8 | 22 | 20 | 16 | 20 | 0 | 34-36 | 34 | 38-40 | 0 | 30-32 | 18-20 | 26 |
observation
- @ref: 18923
- observation: not MRSA
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
68375 | 18257 | ornithine | - | degradation |
68375 | 4853 | esculin | - | hydrolysis |
68375 | 17634 | D-glucose | + | fermentation |
68375 | 15824 | D-fructose | + | fermentation |
68375 | 16024 | D-mannose | + | fermentation |
68375 | 17306 | maltose | + | fermentation |
68375 | 17716 | lactose | + | fermentation |
68375 | 27082 | trehalose | + | fermentation |
68375 | 16899 | D-mannitol | + | fermentation |
68375 | 16634 | raffinose | - | fermentation |
68375 | 16988 | D-ribose | - | fermentation |
68375 | 17057 | cellobiose | - | fermentation |
68375 | 17632 | nitrate | + | reduction |
68375 | 17992 | sucrose | + | fermentation |
68375 | 59640 | N-acetylglucosamine | + | fermentation |
68375 | 32528 | turanose | + | fermentation |
68375 | 22599 | arabinose | - | fermentation |
enzymes
@ref | value | activity | ec |
---|---|---|---|
18923 | catalase | + | 1.11.1.6 |
18923 | cytochrome-c oxidase | - | 1.9.3.1 |
68375 | beta-glucuronidase | - | 3.2.1.31 |
68375 | alkaline phosphatase | + | 3.1.3.1 |
68375 | L-arginine arylamidase | - | |
68375 | beta-galactosidase | - | 3.2.1.23 |
68375 | beta-glucosidase | - | 3.2.1.21 |
68375 | ornithine decarboxylase | - | 4.1.1.17 |
API ID32STA
@ref | URE | ADH Arg | ODC | ESC | GLU | FRU | MNE | MAL | LAC | TRE | MAN | RAF | RIB | CEL | NIT | VP | beta GAL | ArgA | PAL | PyrA | NOVO | SAC | NAG | TUR | ARA | beta GUR |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
18923 | + | + | - | - | + | + | + | + | + | + | + | - | - | - | + | + | - | - | + | + | - | + | + | + | - | - |
18923 | + | - | - | - | + | + | + | + | + | + | + | - | - | - | + | + | - | - | + | - | + | + | + | + | - | - |
18923 | + | - | - | - | + | + | + | + | + | + | + | - | - | - | + | + | - | - | + | - | - | + | + | + | - | - |
18923 | - | + | - | - | + | + | + | + | + | + | + | - | - | - | + | + | - | - | + | - | +/- | + | + | + | - | - |
63395 | - | + | - | - | + | + | + | + | + | + | + | - | - | - | + | - | - | - | + | - | - | + | + | + | - | - |
Isolation, sampling and environmental information
isolation
@ref | country | origin.country | continent |
---|---|---|---|
18923 | United Kingdom | GBR | Europe |
63395 | United Kingdom | GBR | Europe |
116616 | United Kingdom | GBR | Europe |
Safety information
risk assessment
@ref | pathogenicity human | pathogenicity animal | biosafety level | biosafety level comment |
---|---|---|---|---|
18923 | yes | yes | 2 | Risk group (German classification) |
116616 | 2 | Risk group (French classification) |
External links
@ref: 18923
culture collection no.: DSM 27146, NCTC 12493, WDCM 00212, CCUG 67181, CIP 110856, CECT 9152
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh |
---|---|---|---|---|---|---|---|
Pathogenicity | 16787954 | Reversing methicillin resistance in MRSA using a bacterial transforming agent. | Carey BE, Dancer SJ | J Antimicrob Chemother | 10.1093/jac/dkl245 | 2006 | Anti-Bacterial Agents/*pharmacology, Drug Interactions, Methicillin/*pharmacology, Methicillin Resistance/*drug effects, Microbial Sensitivity Tests, Staphylococcus aureus/*drug effects |
Pathogenicity | 21263047 | Resistance and tolerance to tropodithietic acid, an antimicrobial in aquaculture, is hard to select. | Porsby CH, Webber MA, Nielsen KF, Piddock LJ, Gram L | Antimicrob Agents Chemother | 10.1128/AAC.01222-10 | 2011 | Anti-Bacterial Agents/*pharmacology, Drug Resistance, Bacterial, Magnetic Resonance Spectroscopy, Microbial Sensitivity Tests, Roseobacter/drug effects, Salmonella enterica/drug effects, Staphylococcus aureus/drug effects, Tropolone/*analogs & derivatives/pharmacology |
Phylogeny | 33597879 | Antibacterial Activities of Echinops kebericho Mesfin Tuber Extracts and Isolation of the Most Active Compound, Dehydrocostus Lactone. | Deyno S, Mtewa AG, Hope D, Bazira J, Makonnen E, Alele PE | Front Pharmacol | 10.3389/fphar.2020.608672 | 2021 | |
34588904 | Antibacterial potential of Malaysian ethnomedicinal plants against methicillin-susceptible Staphylococcus aureus (MSSA) and methicillin-resistant Staphylococcus aureus (MRSA). | Yi Xin L, Hui Min T, Nur Liyana Mohd Zin P, Pulingam T, Nelson Appaturi J, Parumasivam T | Saudi J Biol Sci | 10.1016/j.sjbs.2021.06.036 | 2021 |
Reference
@id | authors | catalogue | doi/url | title |
---|---|---|---|---|
18923 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 27146) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-27146 | |
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
41602 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/34410 | ||
63395 | Curators of the CCUG | Culture Collection University of Gothenburg (CCUG) (CCUG 67181) | https://www.ccug.se/strain?id=67181 | |
68375 | Automatically annotated from API ID32STA | |||
116616 | Curators of the CIP | Collection of Institut Pasteur (CIP 110856) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20110856 |