Strain identifier

BacDive ID: 23251

Type strain: Yes

Species: Anaerocella delicata

Strain Designation: WN081

Strain history: A. Ueki; Fac. of Agric., Yamagata Univ., Japan; WN081.

NCBI tax ID(s): 433338 (species)

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General

@ref: 19274

BacDive-ID: 23251

DSM-Number: 23595

keywords: 16S sequence, Bacteria, anaerobe, psychrophilic

description: Anaerocella delicata WN081 is an anaerobe, psychrophilic bacterium that was isolated from methanogenic reactor of cattle waste.

NCBI tax id

  • NCBI tax id: 433338
  • Matching level: species

strain history

@refhistory
19274<- A. Ueki, Yamagata Univ., Tsuruoka, Japan; WN081 <- A. Ueki
67770A. Ueki; Fac. of Agric., Yamagata Univ., Japan; WN081.

doi: 10.13145/bacdive23251.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacteroidota
  • domain: Bacteria
  • phylum: Bacteroidota
  • class: Bacteroidia
  • order: Bacteroidales
  • family: Rikenellaceae
  • genus: Anaerocella
  • species: Anaerocella delicata
  • full scientific name: Anaerocella delicata Abe et al. 2013

@ref: 19274

domain: Bacteria

phylum: Bacteroidetes

class: Bacteroidia

order: Bacteroidales

family: Rikenellaceae

genus: Anaerocella

species: Anaerocella delicata

full scientific name: Anaerocella delicata Abe et al. 2013

strain designation: WN081

type strain: yes

Morphology

colony morphology

  • @ref: 19274
  • incubation period: >14 days

Culture and growth conditions

culture medium

  • @ref: 19274
  • name: ANAEROCELLA MEDIUM (DSMZ Medium 1281)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/1281
  • composition: Name: ANAEROCELLA MEDIUM (DSMZ Medium 1281) Composition: Agar 15.0 g/l Trypticase 10.0 g/l Yeast extract 5.0 g/l (NH4)2SO4 0.9 g/l NaCl 0.9 g/l KH2PO4 0.45 g/l K2HPO4 0.45 g/l L-Cysteine HCl x H2O 0.3 g/l Glucose 0.25 g/l Cellobiose 0.25 g/l Maltose 0.25 g/l Starch 0.25 g/l Na2CO3 0.2 g/l CaCl2 x 2 H2O 0.09 g/l MgSO4 x 7 H2O 0.09 g/l Resazurin 0.001 g/l Pyridoxine hydrochloride 0.0003 g/l Nicotinic acid 0.0002 g/l Thiamine-HCl x 2 H2O 0.0002 g/l Calcium pantothenate 0.0001 g/l Vitamin B12 0.0001 g/l p-Aminobenzoic acid 8e-05 g/l D-(+)-biotin 2e-05 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
19274positivegrowth20psychrophilic
67770positivegrowth30mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 19274
  • oxygen tolerance: anaerobe

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
19274methanogenic reactor of cattle wasteHokkaidoJapanJPNAsia
67770Rice-straw residue in a methanogenic reactor treating waste from cattle farmsJapanJPNAsia

isolation source categories

Cat1Cat2Cat3
#Engineered#Bioreactor
#Host#Mammals#Bovinae (Cow, Cattle)
#Host Body Product#Gastrointestinal tract#Feces (Stool)
#Condition#Anoxic (anaerobic)

taxonmaps

  • @ref: 69479
  • File name: preview.99_150221.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_1554;96_21768;97_27173;98_43943;99_150221&stattab=map
  • Last taxonomy: Anaerocella delicata subclade
  • 16S sequence: AB298736
  • Sequence Identity:
  • Total samples: 6
  • soil counts: 1
  • aquatic counts: 3
  • animal counts: 2

Safety information

risk assessment

  • @ref: 19274
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 19274
  • description: Anaerocella delicata gene for 16S rRNA, partial sequence
  • accession: AB298736
  • length: 1451
  • database: ena
  • NCBI tax ID: 433338

GC content

  • @ref: 19274
  • GC-content: 32.3
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 19274

culture collection no.: DSM 23595, JCM 17049

straininfo link

  • @ref: 87474
  • straininfo: 402616

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny23337575Anaerocella delicata gen. nov., sp. nov., a strictly anaerobic bacterium in the phylum Bacteroidetes isolated from a methanogenic reactor of cattle farms.Abe K, Ueki A, Ohtaki Y, Kaku N, Watanabe K, Ueki KJ Gen Appl Microbiol10.2323/jgam.58.4052012Anaerobiosis, Animal Husbandry, Animals, Bacteria, Anaerobic/classification/genetics/isolation & purification/physiology, Bacterial Typing Techniques, Bacteroidetes/*classification/genetics/*isolation & purification/physiology, Base Composition, Bioreactors/*microbiology, Cattle, DNA, Bacterial/analysis/genetics, DNA, Ribosomal/analysis/genetics, Fatty Acids/analysis, Genes, rRNA, Japan, Methane/*metabolism, Molecular Sequence Data, Oryza/microbiology, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Species SpecificityEnzymology
Phylogeny24899658Acetobacteroides hydrogenigenes gen. nov., sp. nov., an anaerobic hydrogen-producing bacterium in the family Rikenellaceae isolated from a reed swamp.Su XL, Tian Q, Zhang J, Yuan XZ, Shi XS, Guo RB, Qiu YLInt J Syst Evol Microbiol10.1099/ijs.0.063917-02014Bacterial Typing Techniques, Bacteroidetes/*classification/genetics/isolation & purification, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Hydrogen/metabolism, Molecular Sequence Data, Phosphatidylethanolamines/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Water Microbiology, *WetlandsGenetics

Reference

@idauthorscataloguedoi/urltitle
19274Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 23595)https://www.dsmz.de/collection/catalogue/details/culture/DSM-23595
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
87474Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID402616.1StrainInfo: A central database for resolving microbial strain identifiers