Strain identifier
BacDive ID: 23151
Type strain: 
Species: Neisseria oralis
Strain Designation: 6332
Strain history: <- W. J. Wolfgang, Wadsworth Center, NYS Dept. Health, USA; strain 6332 <- Bacteriology Lab.
NCBI tax ID(s): 1107316 (species)
version 8.1 (current version)
General
@ref: 19300
BacDive-ID: 23151
DSM-Number: 25276
keywords: 16S sequence, Bacteria, facultative anaerobe, mesophilic, Gram-negative, coccus-shaped
description: Neisseria oralis 6332 is a facultative anaerobe, mesophilic, Gram-negative bacterium that was isolated from human blood, 55-year-old male.
NCBI tax id
- NCBI tax id: 1107316
- Matching level: species
strain history
- @ref: 19300
- history: <- W. J. Wolfgang, Wadsworth Center, NYS Dept. Health, USA; strain 6332 <- Bacteriology Lab.
doi: 10.13145/bacdive23151.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Betaproteobacteria
- order: Neisseriales
- family: Neisseriaceae
- genus: Neisseria
- species: Neisseria oralis
- full scientific name: Neisseria oralis Wolfgang et al. 2013
@ref: 19300
domain: Bacteria
phylum: Proteobacteria
class: Betaproteobacteria
order: Neisseriales
family: Neisseriaceae
genus: Neisseria
species: Neisseria oralis
full scientific name: Neisseria oralis Wolfgang et al. 2013
strain designation: 6332
type strain: yes
Morphology
cell morphology
- @ref: 30674
- gram stain: negative
- cell length: 0.5 µm
- cell width: 0.5 µm
- cell shape: coccus-shaped
- motility: no
colony morphology
- @ref: 19300
- type of hemolysis: gamma
- incubation period: 1-2 days
pigmentation
- @ref: 30674
- production: yes
Culture and growth conditions
culture medium
- @ref: 19300
- name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)
- growth: yes
- link: https://mediadive.dsmz.de/medium/693
- composition: Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
culture temp
- @ref: 19300
- growth: positive
- type: growth
- temperature: 37
- range: mesophilic
Physiology and metabolism
oxygen tolerance
- @ref: 30674
- oxygen tolerance: facultative anaerobe
observation
- @ref: 30674
- observation: aggregates in chains
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 30674 | 17234 | glucose | + | carbon source |
| 30674 | 17306 | maltose | + | carbon source |
| 30674 | 17992 | sucrose | + | carbon source |
| 30674 | 17632 | nitrate | + | reduction |
| 68377 | 17634 | D-glucose | + | builds acid from |
| 68377 | 15824 | D-fructose | + | builds acid from |
| 68377 | 17306 | maltose | + | builds acid from |
| 68377 | 18257 | ornithine | - | degradation |
| 68377 | 16199 | urea | - | hydrolysis |
| 68377 | 27897 | tryptophan | - | energy source |
metabolite production
- @ref: 68377
- Chebi-ID: 35581
- metabolite: indole
- production: no
metabolite tests
- @ref: 68377
- Chebi-ID: 35581
- metabolite: indole
- indole test: -
enzymes
| @ref | value | activity | ec |
|---|---|---|---|
| 19300 | catalase | + | 1.11.1.6 |
| 19300 | cytochrome-c oxidase | + | 1.9.3.1 |
| 30674 | catalase | + | 1.11.1.6 |
| 30674 | cytochrome oxidase | + | 1.9.3.1 |
| 68377 | tryptophan deaminase | - | 4.1.99.1 |
| 68377 | proline-arylamidase | + | 3.4.11.5 |
| 68377 | alkaline phosphatase | - | 3.1.3.1 |
| 68377 | lipase | - | |
| 68377 | urease | - | 3.5.1.5 |
| 68377 | ornithine decarboxylase | - | 4.1.1.17 |
API NH
| @ref | PEN | GLU | FRU | MAL | SAC | ODC | URE | LIP | PAL | beta GAL | ProA | GGT | IND |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 19300 | + | + | + | + | - | - | - | - | - | + | + | - | - |
| 19300 | - | + | + | + | + | - | - | - | - | - | + | + | - |
Isolation, sampling and environmental information
isolation
- @ref: 19300
- sample type: human blood, 55-year-old male
- geographic location: New York, Dutchess Country
- country: USA
- origin.country: USA
- continent: North America
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Host | #Human | #Male |
| #Host Body Product | #Fluids | #Blood |
taxonmaps
- @ref: 69479
- File name: preview.99_7185.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15355;96_42;97_4128;98_5281;99_7185&stattab=map
- Last taxonomy: Neisseria oralis subclade
- 16S sequence: JN104029
- Sequence Identity:
- Total samples: 55114
- soil counts: 1436
- aquatic counts: 2095
- animal counts: 51095
- plant counts: 488
Safety information
risk assessment
- @ref: 19300
- biosafety level: 2
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 19300
- description: Neisseria oralis strain 6332 16S ribosomal RNA gene, partial sequence
- accession: JN104029
- length: 1463
- database: ena
- NCBI tax ID: 1107316
GC content
- @ref: 19300
- GC-content: 52.6
External links
@ref: 19300
culture collection no.: DSM 25276, LMG 26725
straininfo link
- @ref: 87385
- straininfo: 378947
literature
- topic: Phylogeny
- Pubmed-ID: 22798652
- title: Neisseria oralis sp. nov., isolated from healthy gingival plaque and clinical samples.
- authors: Wolfgang WJ, Passaretti TV, Jose R, Cole J, Coorevits A, Carpenter AN, Jose S, Van Landschoot A, Izard J, Kohlerschmidt DJ, Vandamme P, Dewhirst FE, Fisher MA, Musser KA
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijs.0.041731-0
- year: 2012
- mesh: Bacterial Typing Techniques, DNA, Bacterial/genetics, Dental Plaque/*microbiology, Fatty Acids/analysis, Gingiva/*microbiology, Humans, Molecular Sequence Data, Neisseria/*classification/genetics/isolation & purification, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, RNA, Ribosomal, 23S/genetics, Sequence Analysis, DNA, United States
- topic2: Pathogenicity
Reference
| @id | authors | catalogue | doi/url | title | ID_cross_reference | pubmed |
|---|---|---|---|---|---|---|
| 19300 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 25276) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-25276 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 30674 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | 10.1128/mSphere.00237-17 | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 27005 | 28776041 | |
| 68377 | Automatically annotated from API NH | |||||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
| 87385 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID378947.1 | StrainInfo: A central database for resolving microbial strain identifiers |