Strain identifier

BacDive ID: 23112

Type strain: Yes

Species: Tersicoccus phoenicis

Strain Designation: 1P05MA

Strain history: DSM 30849 <-- P. A. Vaishampayan, Jet Propul. Lab., NASA, USA; 1P05MA.

NCBI tax ID(s): 554083 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 19362

BacDive-ID: 23112

DSM-Number: 30849

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-positive, coccus-shaped

description: Tersicoccus phoenicis 1P05MA is an aerobe, mesophilic, Gram-positive bacterium that was isolated from floor of an ISO 8 clean room.

NCBI tax id

  • NCBI tax id: 554083
  • Matching level: species

strain history

@refhistory
19362<- P. A. Vaishampayan, Jet Propulsion Laboratory, NASA; 1P05MA
67770DSM 30849 <-- P. A. Vaishampayan, Jet Propul. Lab., NASA, USA; 1P05MA.

doi: 10.13145/bacdive23112.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Micrococcales
  • family: Micrococcaceae
  • genus: Tersicoccus
  • species: Tersicoccus phoenicis
  • full scientific name: Tersicoccus phoenicis Vaishampayan et al. 2013

@ref: 19362

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Micrococcaceae

genus: Tersicoccus

species: Tersicoccus phoenicis

full scientific name: Tersicoccus phoenicis Vaishampayan et al. 2013

strain designation: 1P05MA

type strain: yes

Morphology

cell morphology

@refgram staincell shapemotilityconfidence
30903positivecoccus-shapedno
69480positive100

pigmentation

  • @ref: 30903
  • production: yes

multimedia

  • @ref: 19362
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_30849.jpg
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

  • @ref: 19362
  • name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/92
  • composition: Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
19362positivegrowth28mesophilic
30903positivegrowth10-65
30903positiveoptimum30mesophilic
67770positivegrowth28mesophilic

culture pH

  • @ref: 30903
  • ability: positive
  • type: optimum
  • pH: 6.75

Physiology and metabolism

oxygen tolerance

  • @ref: 30903
  • oxygen tolerance: aerobe

spore formation

@refspore formationconfidence
30903no
69480no99.989

halophily

  • @ref: 30903
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-2 %

observation

@refobservation
30903aggregates in clumps
67770quinones: MK-8(H2), MK-9(H2)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3090323652dextrin+carbon source
3090328757fructose+carbon source
3090324265gluconate+carbon source
3090317234glucose+carbon source
3090317754glycerol+carbon source
3090324996lactate+carbon source
3090317306maltose+carbon source
3090329864mannitol+carbon source
3090337684mannose+carbon source
3090315361pyruvate+carbon source
3090351850methyl pyruvate+carbon source
3090316634raffinose+carbon source
3090333942ribose+carbon source
3090330911sorbitol+carbon source
3090327082trehalose+carbon source
3090353423tween 40+carbon source
3090353426tween 80+carbon source
3090316704uridine+carbon source
3090318222xylose+carbon source
3090317632nitrate+reduction

enzymes

@refvalueactivityec
30903cytochrome oxidase+1.9.3.1
30903pyrazinamidase+3.5.1.B15

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
19362floor of an ISO 8 clean roomFlorida, Kennedey Space Center, Payload Hazardous Servicing FacilityUSAUSANorth America
67770Spacecraft assembly clean room at Kennedy Space CenterFLUSAUSANorth America

isolation source categories

  • Cat1: #Engineered
  • Cat2: #Built environment
  • Cat3: #Clean room

Safety information

risk assessment

  • @ref: 19362
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 19362
  • description: Tersicoccus phoenicis strain 1P05MA 16S ribosomal RNA gene, partial sequence
  • accession: EU977596
  • length: 1381
  • database: ena
  • NCBI tax ID: 554083

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Tersicoccus phoenicis strain DSM 30849554083.4wgspatric554083
66792Tersicoccus phoenicis 1p05MA2788500076draftimg554083
67770Tersicoccus phoenicis 1p05MAGCA_001968835contigncbi554083

GC content

@refGC-contentmethod
1936270.6high performance liquid chromatography (HPLC)
3090370.6

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes51no
gram-positiveyes92.608no
anaerobicno98.363no
halophileno73.049no
spore-formingno92.497no
glucose-utilyes82.29yes
thermophileno88.852yes
aerobicyes91.053yes
motileno50.694no
flagellatedno93.441yes
glucose-fermentno85.743no

External links

@ref: 19362

culture collection no.: DSM 30849, JCM 31985, NRRL B-59547

straininfo link

  • @ref: 87349
  • straininfo: 402185

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny23223813Description of Tersicoccus phoenicis gen. nov., sp. nov. isolated from spacecraft assembly clean room environments.Vaishampayan P, Moissl-Eichinger C, Pukall R, Schumann P, Sproer C, Augustus A, Roberts AH, Namba G, Cisneros J, Salmassi T, Venkateswaran KInt J Syst Evol Microbiol10.1099/ijs.0.047134-02012Arthrobacter/classification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, *Environment, Controlled, *Environmental Microbiology, Fatty Acids/analysis, Florida, French Guiana, Micrococcaceae/*classification/genetics/isolation & purification, Molecular Sequence Data, Peptidoglycan/analysis, Phospholipids/analysis, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Spacecraft, Vitamin K 2/analogs & derivatives/analysisGenetics
Phylogeny27609499Tersicoccus solisilvae sp., nov., a bacterium isolated from forest soil.Sultanpuram VR, Mothe T, Chintalapati S, Chintalapati VRInt J Syst Evol Microbiol10.1099/ijsem.0.0014702016Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, *Forests, India, Micrococcaceae/*classification/genetics/isolation & purification, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/analogs & derivatives/chemistryTranscriptome
Genetics28360156Draft Genome Sequences of Tersicoccus phoenicis DSM 30849(T), Isolated from a Cleanroom for Spacecraft Assembly, and Tersicoccus sp. Strain Bi-70, Isolated from a Freshwater Lake.Nakajima Y, Yoshizawa S, Nakamura K, Ogura Y, Hayashi T, Kogure KGenome Announc10.1128/genomeA.00079-172017Phylogeny

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmedjournal
19362Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 30849)https://www.dsmz.de/collection/catalogue/details/culture/DSM-30849
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
30903Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2723328776041
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updates33211880Nucleic Acids Res. 49: D498-D508 2020
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
87349Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID402185.1StrainInfo: A central database for resolving microbial strain identifiers