Strain identifier

BacDive ID: 23093

Type strain: Yes

Species: Methylopila musalis

Strain Designation: MUSA

Strain history: <- N. V. Doronina, Inst. Biochem. Physiol. Microorg., RAS, Pushchino, Russia; MUSA

NCBI tax ID(s): 1134781 (species)

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General

@ref: 19321

BacDive-ID: 23093

DSM-Number: 24986

keywords: 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, motile, rod-shaped

description: Methylopila musalis MUSA is an aerobe, mesophilic, Gram-negative bacterium that was isolated from banana fruit of Musa paradisiaca var. sapientum.

NCBI tax id

  • NCBI tax id: 1134781
  • Matching level: species

strain history

  • @ref: 19321
  • history: <- N. V. Doronina, Inst. Biochem. Physiol. Microorg., RAS, Pushchino, Russia; MUSA

doi: 10.13145/bacdive23093.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Hyphomicrobiales
  • family: Methylocystaceae
  • genus: Methylopila
  • species: Methylopila musalis
  • full scientific name: Methylopila musalis Doronina et al. 2013

@ref: 19321

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Rhizobiales

family: Methylocystaceae

genus: Methylopila

species: Methylopila musalis

full scientific name: Methylopila musalis Doronina et al. 2013

strain designation: MUSA

type strain: yes

Morphology

cell morphology

  • @ref: 30687
  • gram stain: negative
  • cell length: 1.75 µm
  • cell width: 0.65 µm
  • cell shape: rod-shaped
  • motility: yes

colony morphology

  • @ref: 62695
  • incubation period: 2-3 days

pigmentation

  • @ref: 30687
  • production: yes

Culture and growth conditions

culture medium

  • @ref: 19321
  • name: MEDIUM K (DSMZ Medium 1122)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/1122
  • composition: Name: MEDIUM K (DSMZ Medium 1122) Composition: Agar 20.0 g/l KH2PO4 2.0 g/l (NH4)2SO4 2.0 g/l NaCl 0.5 g/l MgSO4 x 7 H2O 0.125 g/l FeSO4 x 7 H2O 0.002 g/l Methanol Distilled water

culture temp

@refgrowthtypetemperaturerange
19321positivegrowth28mesophilic
30687positivegrowth16-40
30687positiveoptimum29mesophilic
62695positivegrowth30mesophilic

culture pH

@refabilitytypepHPH range
30687positivegrowth5.5-9.5alkaliphile
30687positiveoptimum7

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
30687aerobe
62695aerobe

spore formation

  • @ref: 30687
  • spore formation: no

halophily

@refsaltgrowthtested relationconcentration
30687NaClpositivegrowth0-2.5 %
30687NaClpositiveoptimum0.5 %

observation

  • @ref: 30687
  • observation: aggregates in clumps

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3068728757fructose+carbon source
3068717234glucose+carbon source

metabolite production

  • @ref: 30687
  • Chebi-ID: 35581
  • metabolite: indole
  • production: yes

metabolite tests

  • @ref: 30687
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: +

enzymes

@refvalueactivityec
30687catalase+1.11.1.6
30687cytochrome oxidase+1.9.3.1
30687urease+3.5.1.5

Isolation, sampling and environmental information

isolation

@refsample typehost speciescountryorigin.countrycontinentsampling date
19321banana fruit of Musa paradisiaca var. sapientumMusa paradisiaca var. sapientumEcuadorECUMiddle and South America
62695BananaEcuadorECUMiddle and South America2009

isolation source categories

Cat1Cat2Cat3
#Host#Plants#Herbaceous plants (Grass,Crops)
#Host Body-Site#Plant#Fruit (Seed)

taxonmaps

  • @ref: 69479
  • File name: preview.99_167369.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_5;96_1101;97_1289;98_1553;99_167369&stattab=map
  • Last taxonomy: Methylopila
  • 16S sequence: JQ173144
  • Sequence Identity:
  • Total samples: 13
  • soil counts: 1
  • aquatic counts: 2
  • animal counts: 8
  • plant counts: 2

Safety information

risk assessment

  • @ref: 19321
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 19321
  • description: Methylopila musalis strain MUSA 16S ribosomal RNA gene, partial sequence
  • accession: JQ173144
  • length: 1307
  • database: ena
  • NCBI tax ID: 1134781

GC content

@refGC-contentmethod
1932164.5thermal denaturation, midpoint method (Tm)
3068764.5

External links

@ref: 19321

culture collection no.: DSM 24986, CCUG 61696, VKM B-2646

straininfo link

  • @ref: 87331
  • straininfo: 402053

literature

  • topic: Phylogeny
  • Pubmed-ID: 22984139
  • title: Methylopila musalis sp. nov., an aerobic, facultatively methylotrophic bacterium isolated from banana fruit.
  • authors: Doronina NV, Kaparullina EN, Bykova TV, Trotsenko YA
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.042028-0
  • year: 2012
  • mesh: Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Ecuador, Fatty Acids/analysis, Fruit/microbiology, Genes, Bacterial, Methylocystaceae/*classification/genetics/isolation & purification, Molecular Sequence Data, Musa/*microbiology, Nucleic Acid Hybridization, Phospholipids/analysis, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Ubiquinone/analysis
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
19321Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 24986)https://www.dsmz.de/collection/catalogue/details/culture/DSM-24986
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
30687Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2701828776041
62695Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 61696)https://www.ccug.se/strain?id=61696
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
87331Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID402053.1StrainInfo: A central database for resolving microbial strain identifiers