Strain identifier

BacDive ID: 23083

Type strain: No

Species: Methanothrix soehngenii

Strain Designation: GP6

Strain history: DSM 3671 <-- G. B. Patel GP6.

NCBI tax ID(s): 990316 (strain), 2223 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 7 (current version):
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 7 (current version)

General

@ref: 18852

BacDive-ID: 23083

DSM-Number: 3671

keywords: anaerobe, Archaea, 16S sequence, genome sequence, mesophilic

description: Methanothrix soehngenii GP6 is an anaerobe, mesophilic archaeon that was isolated from acetic acid enrichment from pear waste digester inoculated with sewage sludge.

NCBI tax id

NCBI tax idMatching level
990316strain
2223species

strain history

doi: 10.13145/bacdive23083.20220920.7

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/archaea
  • keyword: phylum/euryarchaeota
  • domain: Archaea
  • phylum: Euryarchaeota
  • class: Methanomicrobia
  • order: Methanosarcinales
  • family: Methanosaetaceae
  • genus: Methanothrix
  • species: Methanothrix soehngenii
  • full scientific name: Methanothrix soehngenii Huser et al. 1983
  • synonyms

    @refsynonym
    20215Methanosaeta concilii
    20215Methanothrix concilii

@ref: 18852

domain: Archaea

phylum: Euryarchaeota

class: Methanomicrobia

order: Methanosarcinales

family: Methanosaetaceae

genus: Methanothrix

species: Methanothrix soehngenii

full scientific name: Methanothrix soehngenii Huser et al. 1983

strain designation: GP6

type strain: no

Morphology

colony morphology

  • @ref: 18852
  • incubation period: >14 days

multimedia

  • @ref: 18852
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_3671.jpg
  • caption: Medium 334a, 35°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

  • @ref: 18852
  • name: METHANOTHRIX SOEHNGENII MEDIUM (DSMZ Medium 334a)
  • growth: yes
  • link: https://bacmedia.dsmz.de/medium/334a
  • composition: Name: METHANOTHRIX SOEHNGENII MEDIUM (DSMZ Medium 334a) Composition: Na-acetate 6.60836 g/l Na2CO3 1.21477 g/l Na2HPO4 x 2 H2O 0.835763 g/l Na2S x 9 H2O 0.485909 g/l NaCl 0.291545 g/l NH4Cl 0.291545 g/l KH2PO4 0.2138 g/l Nitrilotriacetic acid 0.124393 g/l CaCl2 x 2 H2O 0.1069 g/l MgCl2 x 6 H2O 0.0971817 g/l FeCl3 x 6 H2O 0.0131195 g/l L-alpha-Phosphatidylcholine 0.00485909 g/l Butyl vinyl ether 0.00485909 g/l NiCl2 x 6 H2O 0.00116618 g/l MnCl2 x 4 H2O 0.000971817 g/l ZnCl2 0.000971817 g/l Na2-EDTA 0.000934888 g/l Sodium resazurin 0.000467444 g/l CoCl2 x 6 H2O 0.000291545 g/l CuCl2 0.000291545 g/l Na2MoO4 x 2 H2O 0.000291545 g/l Na2SeO3 x 5 H2O 0.000291545 g/l H3BO3 9.71817e-05 g/l Pyridoxine hydrochloride 9.71817e-05 g/l Nicotinic acid 4.85909e-05 g/l Riboflavin 4.85909e-05 g/l (±)-alpha-Lipoic acid 4.85909e-05 g/l p-Aminobenzoic acid 4.85909e-05 g/l Thiamine HCl 4.85909e-05 g/l Calcium D-(+)-pantothenate 4.85909e-05 g/l Folic acid 1.94363e-05 g/l Biotin 1.94363e-05 g/l Vitamin B12 9.71817e-07 g/l Methanol Cell-free culture supernatant of S. associata Distilled water

culture temp

@refgrowthtypetemperaturerange
18852positivegrowth35mesophilic
67770positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 18852
  • oxygen tolerance: anaerobe

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
18852acetic acid enrichment from pear waste digester inoculated with sewage sludgeOttawaCanadaCANNorth America
67770Sewage sludgeOttawaCanadaCANNorth America

isolation source categories

Cat1Cat2Cat3
#Engineered#Waste#Sewage sludge
#Host#Plants#Decomposing plant
#Condition#Acidic

Safety information

risk assessment

  • @ref: 18852
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Methanosaeta concilii gene for 16S rRNA, partial sequence, strain: NBRC 103675AB6791681407ena2223
2021816S rRNA [Methanothrix soehngenii, GP6, DSM 3671, rRNA Partial, 159 nt, segment 1 of 3]S42679159ena2223
2021816S rRNA [Methanothrix soehngenii, GP6, DSM 3671, rRNA Partial, 194 nt, segment 2 of 3]S42680194ena2223
2021816S rRNA [Methanothrix soehngenii, GP6, DSM 3671, rRNA Partial, 186 nt, segment 3 of 3]S42681186ena2223

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Methanothrix soehngenii GP6 GP6GCA_000204415completencbi990316
67770Methanosaeta concilii GP-6, complete genomeCP002565ena990316

GC content

@refGC-contentmethod
1885261.0
1885250.2
6777061fluorimetric

External links

@ref: 18852

culture collection no.: DSM 3671, JCM 10134, NBRC 103675, OCM 69, ATCC 35969, NRC 2989

straininfo link

@refpassport
20218http://www.straininfo.net/strains/158492
20218http://www.straininfo.net/strains/158494
20218http://www.straininfo.net/strains/802484

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
18852Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 3671)https://www.dsmz.de/collection/catalogue/details/culture/DSM-3671
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/