Strain identifier

BacDive ID: 23078

Type strain: Yes

Species: Listeria weihenstephanensis

Strain Designation: WS 4560

Strain history: <- S. Scherer, Research Center for Nutrition and Food Sci. (ZIEL), TU Munich, Germany; WS 4560 <- E. Lang-Halter

NCBI tax ID(s): 1006155 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 19220

BacDive-ID: 23078

DSM-Number: 24698

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic, Gram-positive, motile, rod-shaped

description: Listeria weihenstephanensis WS 4560 is an anaerobe, mesophilic, Gram-positive bacterium that was isolated from water plant Lemna trisulca from pond.

NCBI tax id

  • NCBI tax id: 1006155
  • Matching level: species

strain history

  • @ref: 19220
  • history: <- S. Scherer, Research Center for Nutrition and Food Sci. (ZIEL), TU Munich, Germany; WS 4560 <- E. Lang-Halter

doi: 10.13145/bacdive23078.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Listeriaceae
  • genus: Listeria
  • species: Listeria weihenstephanensis
  • full scientific name: Listeria weihenstephanensis Lang Halter et al. 2013

@ref: 19220

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Listeriaceae

genus: Listeria

species: Listeria weihenstephanensis

full scientific name: Listeria weihenstephanensis Lang Halter et al. 2013

strain designation: WS 4560

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
30470positive3.25 µm0.45 µmrod-shapedyes
69480positive100

colony morphology

  • @ref: 19220
  • type of hemolysis: gamma
  • incubation period: 1-2 days

pigmentation

  • @ref: 30470
  • production: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
19220BHI MEDIUM (DSMZ Medium 215)yeshttps://mediadive.dsmz.de/medium/215Name: BHI MEDIUM (DSMZ Medium 215) Composition: Brain heart infusion 37.0 g/l Distilled water
19220COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base

culture temp

@refgrowthtypetemperaturerange
19220positivegrowth30mesophilic
30470positivegrowth15-34
30470positiveoptimum31mesophilic

culture pH

  • @ref: 30470
  • ability: positive
  • type: optimum
  • pH: 7.5

Physiology and metabolism

oxygen tolerance

  • @ref: 30470
  • oxygen tolerance: anaerobe

spore formation

@refspore formationconfidence
30470no
69480no99.371

halophily

  • @ref: 30470
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-6.5 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
30470581435-dehydro-D-gluconate+carbon source
3047018403L-arabitol+carbon source
3047017057cellobiose+carbon source
3047028757fructose+carbon source
3047017234glucose+carbon source
3047017754glycerol+carbon source
3047028087glycogen+carbon source
3047017716lactose+carbon source
3047017306maltose+carbon source
3047029864mannitol+carbon source
3047037684mannose+carbon source
30470506227N-acetylglucosamine+carbon source
3047026546rhamnose+carbon source
3047033942ribose+carbon source
3047017814salicin+carbon source
3047027082trehalose+carbon source
3047017151xylitol+carbon source
3047018222xylose+carbon source
304704853esculin+hydrolysis
3047017632nitrate+reduction
683764853esculin+hydrolysis
6837618333D-arabitol+builds acid from
6837665327D-xylose+builds acid from
6837662345L-rhamnose+builds acid from
68376320061methyl alpha-D-glucopyranoside+builds acid from
6837629042glucose 1-phosphate-builds acid from
6837616443D-tagatose-builds acid from

enzymes

@refvalueactivityec
19220catalase+1.11.1.6
19220cytochrome-c oxidase-1.9.3.1
30470catalase+1.11.1.6
68376alpha-mannosidase-3.2.1.24
68376beta-glucosidase+3.2.1.21

API LIST

@refDIMESCalpha MANDARLXYLRHAMDGRIBG1PTAG
19220-+-+++++/---
19220-+-++++---

Isolation, sampling and environmental information

isolation

  • @ref: 19220
  • sample type: water plant Lemna trisulca from pond
  • host species: Lemna trisulca
  • geographic location: Bavaria, Wolnzach/Pfaffenhofen a. d. Ilm, pond Postweiher
  • country: Germany
  • origin.country: DEU
  • continent: Europe

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Pond (small)
#Host#Plants#Aquatic plant

Safety information

risk assessment

  • @ref: 19220
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 19220
  • description: Listeria weihenstephanensis partial 16S rRNA gene, type strain DSM 24698T
  • accession: FR850019
  • length: 1514
  • database: ena
  • NCBI tax ID: 1006155

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Listeria weihenstephanensis strain WS 45601006155.5completepatric1006155
66792Listeria weihenstephanensis WS 4560GCA_003534205completencbi1006155
66792Listeria weihenstephanensis strain WS 45601006155.8completepatric1006155
66792Listeria weihenstephanensis strain WS 45601006155.7completepatric1006155
66792Listeria weihenstephanensis strain WS 45601006155.6completepatric1006155

GC content

@refGC-contentmethod
1922041.8high performance liquid chromatography (HPLC)
3047041.8

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes50no
motileno84.814yes
flagellatedno94.064no
gram-positiveyes95.992yes
anaerobicno93.326yes
aerobicno88.046yes
halophileyes54.59no
spore-formingno84.536yes
thermophileno99.724yes
glucose-utilyes89.099yes
glucose-fermentyes65.006no

External links

@ref: 19220

culture collection no.: DSM 24698, LMG 26374

straininfo link

  • @ref: 87316
  • straininfo: 377130

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny22544790Listeria weihenstephanensis sp. nov., isolated from the water plant Lemna trisulca taken from a freshwater pond.Lang Halter E, Neuhaus K, Scherer SInt J Syst Evol Microbiol10.1099/ijs.0.036830-02012Araceae/*microbiology, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/analysis, Genes, Bacterial, Germany, Listeria/*classification/genetics/isolation & purification, Molecular Sequence Data, Nucleic Acid Hybridization, *Phylogeny, Ponds, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/analysisGenetics
Genetics28919887Complete Circular Genome Sequence and Temperature Independent Adaptation to Anaerobiosis of Listeria weihenstephanensis DSM 24698.Ferrari E, Walter MC, Huptas C, Scherer S, Muller-Herbst SFront Microbiol10.3389/fmicb.2017.016722017

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
19220Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 24698)https://www.dsmz.de/collection/catalogue/details/culture/DSM-24698
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
30470Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2680528776041
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68376Automatically annotated from API LIST
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
87316Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID377130.1StrainInfo: A central database for resolving microbial strain identifiers