Strain identifier
BacDive ID: 23078
Type strain:
Species: Listeria weihenstephanensis
Strain Designation: WS 4560
Strain history: <- S. Scherer, Research Center for Nutrition and Food Sci. (ZIEL), TU Munich, Germany; WS 4560 <- E. Lang-Halter
NCBI tax ID(s): 1006155 (species)
General
@ref: 19220
BacDive-ID: 23078
DSM-Number: 24698
keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic, Gram-positive, motile, rod-shaped
description: Listeria weihenstephanensis WS 4560 is an anaerobe, mesophilic, Gram-positive bacterium that was isolated from water plant Lemna trisulca from pond.
NCBI tax id
- NCBI tax id: 1006155
- Matching level: species
strain history
- @ref: 19220
- history: <- S. Scherer, Research Center for Nutrition and Food Sci. (ZIEL), TU Munich, Germany; WS 4560 <- E. Lang-Halter
doi: 10.13145/bacdive23078.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Bacilli
- order: Caryophanales
- family: Listeriaceae
- genus: Listeria
- species: Listeria weihenstephanensis
- full scientific name: Listeria weihenstephanensis Lang Halter et al. 2013
@ref: 19220
domain: Bacteria
phylum: Firmicutes
class: Bacilli
order: Bacillales
family: Listeriaceae
genus: Listeria
species: Listeria weihenstephanensis
full scientific name: Listeria weihenstephanensis Lang Halter et al. 2013
strain designation: WS 4560
type strain: yes
Morphology
cell morphology
@ref | gram stain | cell length | cell width | cell shape | motility | confidence |
---|---|---|---|---|---|---|
30470 | positive | 3.25 µm | 0.45 µm | rod-shaped | yes | |
69480 | positive | 100 |
colony morphology
- @ref: 19220
- type of hemolysis: gamma
- incubation period: 1-2 days
pigmentation
- @ref: 30470
- production: yes
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
19220 | BHI MEDIUM (DSMZ Medium 215) | yes | https://mediadive.dsmz.de/medium/215 | Name: BHI MEDIUM (DSMZ Medium 215) Composition: Brain heart infusion 37.0 g/l Distilled water |
19220 | COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) | yes | https://mediadive.dsmz.de/medium/693 | Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
19220 | positive | growth | 30 | mesophilic |
30470 | positive | growth | 15-34 | |
30470 | positive | optimum | 31 | mesophilic |
culture pH
- @ref: 30470
- ability: positive
- type: optimum
- pH: 7.5
Physiology and metabolism
oxygen tolerance
- @ref: 30470
- oxygen tolerance: anaerobe
spore formation
@ref | spore formation | confidence |
---|---|---|
30470 | no | |
69480 | no | 99.371 |
halophily
- @ref: 30470
- salt: NaCl
- growth: positive
- tested relation: growth
- concentration: 0-6.5 %
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
30470 | 58143 | 5-dehydro-D-gluconate | + | carbon source |
30470 | 18403 | L-arabitol | + | carbon source |
30470 | 17057 | cellobiose | + | carbon source |
30470 | 28757 | fructose | + | carbon source |
30470 | 17234 | glucose | + | carbon source |
30470 | 17754 | glycerol | + | carbon source |
30470 | 28087 | glycogen | + | carbon source |
30470 | 17716 | lactose | + | carbon source |
30470 | 17306 | maltose | + | carbon source |
30470 | 29864 | mannitol | + | carbon source |
30470 | 37684 | mannose | + | carbon source |
30470 | 506227 | N-acetylglucosamine | + | carbon source |
30470 | 26546 | rhamnose | + | carbon source |
30470 | 33942 | ribose | + | carbon source |
30470 | 17814 | salicin | + | carbon source |
30470 | 27082 | trehalose | + | carbon source |
30470 | 17151 | xylitol | + | carbon source |
30470 | 18222 | xylose | + | carbon source |
30470 | 4853 | esculin | + | hydrolysis |
30470 | 17632 | nitrate | + | reduction |
68376 | 4853 | esculin | + | hydrolysis |
68376 | 18333 | D-arabitol | + | builds acid from |
68376 | 65327 | D-xylose | + | builds acid from |
68376 | 62345 | L-rhamnose | + | builds acid from |
68376 | 320061 | methyl alpha-D-glucopyranoside | + | builds acid from |
68376 | 29042 | glucose 1-phosphate | - | builds acid from |
68376 | 16443 | D-tagatose | - | builds acid from |
enzymes
@ref | value | activity | ec |
---|---|---|---|
19220 | catalase | + | 1.11.1.6 |
19220 | cytochrome-c oxidase | - | 1.9.3.1 |
30470 | catalase | + | 1.11.1.6 |
68376 | alpha-mannosidase | - | 3.2.1.24 |
68376 | beta-glucosidase | + | 3.2.1.21 |
API LIST
@ref | DIM | ESC | alpha MAN | DARL | XYL | RHA | MDG | RIB | G1P | TAG |
---|---|---|---|---|---|---|---|---|---|---|
19220 | - | + | - | + | + | + | + | +/- | - | - |
19220 | - | + | - | + | + | + | + | - | - | - |
Isolation, sampling and environmental information
isolation
- @ref: 19220
- sample type: water plant Lemna trisulca from pond
- host species: Lemna trisulca
- geographic location: Bavaria, Wolnzach/Pfaffenhofen a. d. Ilm, pond Postweiher
- country: Germany
- origin.country: DEU
- continent: Europe
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Environmental | #Aquatic | #Pond (small) |
#Host | #Plants | #Aquatic plant |
Safety information
risk assessment
- @ref: 19220
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 19220
- description: Listeria weihenstephanensis partial 16S rRNA gene, type strain DSM 24698T
- accession: FR850019
- length: 1514
- database: ena
- NCBI tax ID: 1006155
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Listeria weihenstephanensis strain WS 4560 | 1006155.5 | complete | patric | 1006155 |
66792 | Listeria weihenstephanensis WS 4560 | GCA_003534205 | complete | ncbi | 1006155 |
66792 | Listeria weihenstephanensis strain WS 4560 | 1006155.8 | complete | patric | 1006155 |
66792 | Listeria weihenstephanensis strain WS 4560 | 1006155.7 | complete | patric | 1006155 |
66792 | Listeria weihenstephanensis strain WS 4560 | 1006155.6 | complete | patric | 1006155 |
GC content
@ref | GC-content | method |
---|---|---|
19220 | 41.8 | high performance liquid chromatography (HPLC) |
30470 | 41.8 |
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
spore-forming | yes | 50 | no |
motile | no | 84.814 | yes |
flagellated | no | 94.064 | no |
gram-positive | yes | 95.992 | yes |
anaerobic | no | 93.326 | yes |
aerobic | no | 88.046 | yes |
halophile | yes | 54.59 | no |
spore-forming | no | 84.536 | yes |
thermophile | no | 99.724 | yes |
glucose-util | yes | 89.099 | yes |
glucose-ferment | yes | 65.006 | no |
External links
@ref: 19220
culture collection no.: DSM 24698, LMG 26374
straininfo link
- @ref: 87316
- straininfo: 377130
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 22544790 | Listeria weihenstephanensis sp. nov., isolated from the water plant Lemna trisulca taken from a freshwater pond. | Lang Halter E, Neuhaus K, Scherer S | Int J Syst Evol Microbiol | 10.1099/ijs.0.036830-0 | 2012 | Araceae/*microbiology, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/analysis, Genes, Bacterial, Germany, Listeria/*classification/genetics/isolation & purification, Molecular Sequence Data, Nucleic Acid Hybridization, *Phylogeny, Ponds, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/analysis | Genetics |
Genetics | 28919887 | Complete Circular Genome Sequence and Temperature Independent Adaptation to Anaerobiosis of Listeria weihenstephanensis DSM 24698. | Ferrari E, Walter MC, Huptas C, Scherer S, Muller-Herbst S | Front Microbiol | 10.3389/fmicb.2017.01672 | 2017 |
Reference
@id | authors | catalogue | doi/url | title | ID_cross_reference | pubmed |
---|---|---|---|---|---|---|
19220 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 24698) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-24698 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
30470 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | 10.1128/mSphere.00237-17 | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 26805 | 28776041 | |
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
68376 | Automatically annotated from API LIST | |||||
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
87316 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID377130.1 | StrainInfo: A central database for resolving microbial strain identifiers |