Strain identifier

BacDive ID: 2302

Type strain: Yes

Species: Brevundimonas vesicularis

Strain Designation: 13alt, Kosako 85012, Otto A117

Variant: Colony variant of BacDive ID 2301

Strain history: CIP <- 1984, LMG, Pseudomonas vesicularis <- ATCC <- G.J. Jann <- E. Morse <- R.S. Breed <- K. Busing

NCBI tax ID(s): 1349759 (strain), 41276 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 3068

BacDive-ID: 2302

DSM-Number: 7233

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, mesophilic, Gram-negative

description: Brevundimonas vesicularis 13alt is an obligate aerobe, mesophilic, Gram-negative bacterium that was isolated from Leech , urinary-bladder epithelium.

NCBI tax id

NCBI tax idMatching level
1349759strain
41276species

strain history

@refhistory
3068<- LMG <- ATCC (Pseudomonas vesiculare) <- G. Jann
67770Y. Kosako 85012 <-- Nissui Pharm. Co., Ltd. <-- ATCC 11426 <-- G. J. Jann <-- E. Morse <-- R. Breed <-- K. Büsing.
123827CIP <- 1984, LMG, Pseudomonas vesicularis <- ATCC <- G.J. Jann <- E. Morse <- R.S. Breed <- K. Busing

doi: 10.13145/bacdive2302.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Caulobacterales
  • family: Caulobacteraceae
  • genus: Brevundimonas
  • species: Brevundimonas vesicularis
  • full scientific name: Brevundimonas vesicularis (Büsing et al. 1953) Segers et al. 1994

  • synonyms

    @refsynonym
    20215Corynebacterium vesiculare
    20215Pseudomonas vesicularis

@ref: 3068

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Rhizobiales

family: Caulobacteraceae

genus: Brevundimonas

species: Brevundimonas vesicularis

full scientific name: Brevundimonas vesicularis (Büsing et al. 1953) Segers et al. 1994

strain designation: 13alt, Kosako 85012, Otto A117

variant: Colony variant of BacDive ID 2301

type strain: yes

Morphology

cell morphology

@refgram stainconfidencecell shapemotility
69480negative99.991
123827negativerod-shapedyes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
3068BLOOD AGAR BASE (OXOID CM55) (DSMZ Medium 617a)yeshttps://mediadive.dsmz.de/medium/617aName: BLOOD AGAR BASE (OXOID CM55) (DSMZ Medium 617a) Composition: Agar 15.0 g/l Beef extract 10.0 g/l Peptone 10.0 g/l NaCl 5.0 g/l Tap water
3068NUTRIENT AGAR (DSMZ Medium 1)yeshttps://mediadive.dsmz.de/medium/1Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
123827CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
3068positivegrowth26mesophilic
67770positivegrowth26mesophilic
123827positivegrowth5-30
123827nogrowth37mesophilic
123827nogrowth41thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 123827
  • oxygen tolerance: obligate aerobe

spore formation

@refspore formationconfidence
69481no99
69480no99.999

halophily

@refsaltgrowthtested relationconcentration
123827NaClpositivegrowth0-2 %
123827NaClnogrowth4 %
123827NaClnogrowth6 %
123827NaClnogrowth8 %
123827NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1238274853esculin-hydrolysis
12382717632nitrate-reduction
12382716301nitrite-reduction

antibiotic resistance

  • @ref: 123827
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

  • @ref: 123827
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase+3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin+3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
123827oxidase-
123827beta-galactosidase+3.2.1.23
123827alcohol dehydrogenase-1.1.1.1
123827gelatinase+/-
123827amylase+
123827DNase+
123827caseinase+3.4.21.50
123827catalase+1.11.1.6
123827tween esterase-
123827lecithinase-
123827lipase-
123827lysine decarboxylase-4.1.1.18
123827ornithine decarboxylase-4.1.1.17
123827protease+
123827tryptophan deaminase-
123827urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
123827-+++-++-+-++--+-----

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
123827+-+------++----+--+------------------------------+-------------------------+---++-------++++-------

Isolation, sampling and environmental information

isolation

@refsample typehost species
3068Leech (Hirudo), urinary-bladder epitheliumHirudo
67770Urinary-bladder epithelium of leech (Hirudo)Hirudo
123827Animal, Leech, urinary-bladder epithelium

isolation source categories

Cat1Cat2Cat3
#Host#Invertebrates (Other)#Annelida
#Host Body-Site#Urogenital tract#Bladder

taxonmaps

  • @ref: 69479
  • File name: preview.99_257.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_73;96_180;97_196;98_219;99_257&stattab=map
  • Last taxonomy: Brevundimonas
  • 16S sequence: AJ007801
  • Sequence Identity:
  • Total samples: 21188
  • soil counts: 1940
  • aquatic counts: 4334
  • animal counts: 9723
  • plant counts: 5191

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
30682Risk group (German classification)
1238271Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Brevundimonas vesicularis 16S rRNA geneAJ0078011390ena41276
20218Brevundimonas vesicularis strain CIP 101035 16S ribosomal RNA gene, partial sequenceEU0241371260ena41276
20218Brevundimonas vesicularis gene for 16S rRNA, strain: IAM 12105AB0214141450ena41276
20218Brevundimonas vesicularis DNA for 16S ribosomal RNA, strain LMG 2350 (T)AJ2277801416ena41276
20218Brevundimonas vesicularis gene for 16S rRNA, partial sequence, strain: NBRC 12165AB6802471386ena41276

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Brevundimonas vesicularis NBRC 121651349759.3wgspatric1349759
66792Brevundimonas vesicularis NBRC 121652731957683draftimg1349759
67770Brevundimonas vesicularis NBRC 12165GCA_001592205contigncbi1349759

GC content

  • @ref: 67770
  • GC-content: 65.8
  • method: thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno99no
motileyes89.844no
gram-positiveno98.23no
anaerobicno97.206no
halophileno95.12no
spore-formingno95.347no
glucose-utilyes78.878no
aerobicyes90.478no
flagellatedno80.704no
thermophileno99.531no
glucose-fermentno92.524no

External links

@ref: 3068

culture collection no.: DSM 7233, ATCC 11426, CCEB 857, CCM 3398, CCUG 2032, CECT 327, IAM 12105, JCM 1477, NCMB 1945, NCTC 10900, Stanier 500, BCRC 11012, CGMCC 1.7034, CIP 101035, DSM 7226, IFO 12165, LMG 2350, NBRC 12165, NCIMB 1945, VKM B-974, VTT E-981070

straininfo link

  • @ref: 71859
  • straininfo: 85200

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Pathogenicity1921759Neutralization of Shwartzman-inducing activity by antibodies recognizing the Re core or lipid A structures of lipopolysaccharide from Salmonella minnesota R595 and Pseudomonas vesicularis JCM1477.Mashimo J, Mizutani T, Mita A, Kasai NMicrobiol Immunol10.1111/j.1348-0421.1991.tb01573.x1991Animals, Antibodies, Bacterial/*immunology, Antibodies, Monoclonal, Endotoxins/*immunology, Enzyme-Linked Immunosorbent Assay, Immunoglobulin G/immunology, Lipid A/*immunology, Lipopolysaccharides/immunology, Mice, Mice, Inbred BALB C, Pseudomonas/*immunology, Rabbits, Salmonella/*immunology, Shwartzman Phenomenon
Enzymology2614089[A strain of Pseudomonas vesicularis isolated from shower hose which supports the multiplication of Legionella].Koide M, Miyata T, Nukina M, Teramoto T, Nakanishi H, Kamiki T, Umeda B, Nakai HKansenshogaku Zasshi10.11150/kansenshogakuzasshi1970.63.11601989Legionella/*growth & development, Pseudomonas/growth & development/*isolation & purification, *Sanitary Engineering, *Water MicrobiologyPhylogeny
Phylogeny2777066Isolation of 9-hydroxy-delta-tetradecalactone from lipid A of Pseudomonas diminuta and Pseudomonas vesicularis.Arata S, Hirayama T, Kasai N, Itoh T, Ohsawa AFEMS Microbiol Lett10.1016/0378-1097(89)90512-01989Lactones/*isolation & purification, Lipid A/*analysis, Magnetic Resonance Spectroscopy, Mass Spectrometry, Molecular Structure, Pseudomonas/*analysisEnzymology
Enzymology3057331Structural heterogeneity regarding local Shwartzman activity of lipid A.Mashimo J, Tanaka C, Arata S, Akiyama Y, Hata S, Hirayama T, Egawa K, Kasai NMicrobiol Immunol10.1111/j.1348-0421.1988.tb01427.x1988Animals, Chromobacterium/analysis, Escherichia coli/analysis, Female, Lipid A/isolation & purification/*pharmacology, Molecular Structure, Pseudomonas/analysis, Rabbits, Salmonella/analysis, Shwartzman Phenomenon/*etiology, Structure-Activity RelationshipPhylogeny
Phylogeny16902022Meganema perideroedes gen. nov., sp. nov., a filamentous alphaproteobacterium from activated sludge.Thomsen TR, Blackall LL, de Muro MA, Nielsen JL, Nielsen PHInt J Syst Evol Microbiol10.1099/ijs.0.02916-02006Alphaproteobacteria/chemistry/*classification/isolation & purification/physiology, Base Composition, Culture Media, DNA, Bacterial/genetics, Denmark, Fatty Acids/analysis, Industrial Waste/analysis, Molecular Sequence Data, Oligonucleotide Probes, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Homology, Nucleic Acid, Sewage/*microbiology, Sodium Chloride, Species Specificity, Temperature, Ubiquinone/analysisCultivation
Phylogeny26674350Brevundimonas albigilva sp. nov., isolated from forest soil.Pham VHT, Jeong S, Chung S, Kim JInt J Syst Evol Microbiol10.1099/ijsem.0.0008482015
Phylogeny27995874Brevundimonas balnearis sp. nov., isolated from the well water of a thermal bath.Toth E, Szuroczki S, Keki Z, Kosztik J, Makk J, Boka K, Sproer C, Marialigeti K, Schumann PInt J Syst Evol Microbiol10.1099/ijsem.0.0017462017Bacterial Typing Techniques, Base Composition, Caulobacteraceae/*classification/genetics/isolation & purification, DNA, Bacterial/genetics, Fatty Acids/chemistry, Hungary, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Temperature, Ubiquinone/analogs & derivatives/chemistry, *Water Microbiology, *Water WellsTranscriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
3068Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 7233)https://www.dsmz.de/collection/catalogue/details/culture/DSM-7233
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
71859Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID85200.1StrainInfo: A central database for resolving microbial strain identifiers
123827Curators of the CIPCollection of Institut Pasteur (CIP 101035)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20101035