Strain identifier

BacDive ID: 2301

Type strain: Yes

Species: Brevundimonas vesicularis

Strain Designation: 13alt, t2, t2

Variant: Colony variant of BacDive ID 2302

Strain history: <- LMG <- ATCC (Pseudomonas vesicularis) <- G. Jann

NCBI tax ID(s): 41276 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 3061

BacDive-ID: 2301

DSM-Number: 7226

keywords: 16S sequence, Bacteria, mesophilic

description: Brevundimonas vesicularis 13alt is a mesophilic bacterium that was isolated from leech , urinary-bladder epithelium..

NCBI tax id

  • NCBI tax id: 41276
  • Matching level: species

strain history

  • @ref: 3061
  • history: <- LMG <- ATCC (Pseudomonas vesicularis) <- G. Jann

doi: 10.13145/bacdive2301.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Caulobacterales
  • family: Caulobacteraceae
  • genus: Brevundimonas
  • species: Brevundimonas vesicularis
  • full scientific name: Brevundimonas vesicularis (Büsing et al. 1953) Segers et al. 1994

  • synonyms

    @refsynonym
    20215Corynebacterium vesiculare
    20215Pseudomonas vesicularis

@ref: 3061

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Rhizobiales

family: Caulobacteraceae

genus: Brevundimonas

species: Brevundimonas vesicularis

full scientific name: Brevundimonas vesicularis (Büsing et al. 1953) Segers et al. 1994

strain designation: 13alt, t2, t2

variant: Colony variant of BacDive ID 2302

type strain: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
3061NUTRIENT AGAR (DSMZ Medium 1)yeshttps://mediadive.dsmz.de/medium/1Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
3061BLOOD AGAR BASE (OXOID CM55) (DSMZ Medium 617a)yeshttps://mediadive.dsmz.de/medium/617aName: BLOOD AGAR BASE (OXOID CM55) (DSMZ Medium 617a) Composition: Agar 15.0 g/l Beef extract 10.0 g/l Peptone 10.0 g/l NaCl 5.0 g/l Tap water
35302MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)

culture temp

@refgrowthtypetemperaturerange
3061positivegrowth26mesophilic
35302positivegrowth25mesophilic

Physiology and metabolism

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate+assimilation
6836917128adipate-assimilation
6836927689decanoate-assimilation
6836924265gluconate-assimilation
6836917306maltose+assimilation
6836959640N-acetylglucosamine-assimilation
6836916899D-mannitol-assimilation
6836916024D-mannose-assimilation
6836930849L-arabinose-assimilation
6836917634D-glucose+assimilation
683695291gelatin-hydrolysis
683694853esculin+hydrolysis
6836916199urea-hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate-reduction

metabolite production

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin+3.4.21.1
68382trypsin+3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)-
68382alkaline phosphatase+3.1.3.1
68369gelatinase-
68369beta-glucosidase+3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase-3.5.3.6

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
65626-+-+-++-++++---+----

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPAC
65626-----+--+----+---+--

Isolation, sampling and environmental information

isolation

  • @ref: 3061
  • sample type: leech (Hirudo), urinary-bladder epithelium.
  • host species: Hirudo

isolation source categories

Cat1Cat2Cat3
#Host#Invertebrates (Other)#Annelida
#Host Body-Site#Urogenital tract#Bladder

taxonmaps

  • @ref: 69479
  • File name: preview.99_257.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_73;96_180;97_196;98_219;99_257&stattab=map
  • Last taxonomy: Brevundimonas
  • 16S sequence: LN681560
  • Sequence Identity:
  • Total samples: 21188
  • soil counts: 1940
  • aquatic counts: 4334
  • animal counts: 9723
  • plant counts: 5191

Safety information

risk assessment

  • @ref: 3061
  • biosafety level: 2
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 3061
  • description: Brevundimonas vesicularis partial 16S rRNA gene, type strain DSM 7226T
  • accession: LN681560
  • length: 1413
  • database: ena
  • NCBI tax ID: 41276

External links

@ref: 3061

culture collection no.: DSM 7226, ATCC 11426, CCM 3398, CCUG 2032, CECT 327, IAM 12105, JCM 1477, LMG 2350, NCMB 1945, NCTC 10900

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Pathogenicity1921759Neutralization of Shwartzman-inducing activity by antibodies recognizing the Re core or lipid A structures of lipopolysaccharide from Salmonella minnesota R595 and Pseudomonas vesicularis JCM1477.Mashimo J, Mizutani T, Mita A, Kasai NMicrobiol Immunol10.1111/j.1348-0421.1991.tb01573.x1991Animals, Antibodies, Bacterial/*immunology, Antibodies, Monoclonal, Endotoxins/*immunology, Enzyme-Linked Immunosorbent Assay, Immunoglobulin G/immunology, Lipid A/*immunology, Lipopolysaccharides/immunology, Mice, Mice, Inbred BALB C, Pseudomonas/*immunology, Rabbits, Salmonella/*immunology, Shwartzman Phenomenon
Enzymology2614089[A strain of Pseudomonas vesicularis isolated from shower hose which supports the multiplication of Legionella].Koide M, Miyata T, Nukina M, Teramoto T, Nakanishi H, Kamiki T, Umeda B, Nakai HKansenshogaku Zasshi10.11150/kansenshogakuzasshi1970.63.11601989Legionella/*growth & development, Pseudomonas/growth & development/*isolation & purification, *Sanitary Engineering, *Water MicrobiologyPhylogeny
Phylogeny2777066Isolation of 9-hydroxy-delta-tetradecalactone from lipid A of Pseudomonas diminuta and Pseudomonas vesicularis.Arata S, Hirayama T, Kasai N, Itoh T, Ohsawa AFEMS Microbiol Lett10.1016/0378-1097(89)90512-01989Lactones/*isolation & purification, Lipid A/*analysis, Magnetic Resonance Spectroscopy, Mass Spectrometry, Molecular Structure, Pseudomonas/*analysisEnzymology
Enzymology3057331Structural heterogeneity regarding local Shwartzman activity of lipid A.Mashimo J, Tanaka C, Arata S, Akiyama Y, Hata S, Hirayama T, Egawa K, Kasai NMicrobiol Immunol10.1111/j.1348-0421.1988.tb01427.x1988Animals, Chromobacterium/analysis, Escherichia coli/analysis, Female, Lipid A/isolation & purification/*pharmacology, Molecular Structure, Pseudomonas/analysis, Rabbits, Salmonella/analysis, Shwartzman Phenomenon/*etiology, Structure-Activity RelationshipPhylogeny
Phylogeny16902022Meganema perideroedes gen. nov., sp. nov., a filamentous alphaproteobacterium from activated sludge.Thomsen TR, Blackall LL, de Muro MA, Nielsen JL, Nielsen PHInt J Syst Evol Microbiol10.1099/ijs.0.02916-02006Alphaproteobacteria/chemistry/*classification/isolation & purification/physiology, Base Composition, Culture Media, DNA, Bacterial/genetics, Denmark, Fatty Acids/analysis, Industrial Waste/analysis, Molecular Sequence Data, Oligonucleotide Probes, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Homology, Nucleic Acid, Sewage/*microbiology, Sodium Chloride, Species Specificity, Temperature, Ubiquinone/analysisCultivation
Phylogeny26674350Brevundimonas albigilva sp. nov., isolated from forest soil.Pham VHT, Jeong S, Chung S, Kim JInt J Syst Evol Microbiol10.1099/ijsem.0.0008482015
Phylogeny27995874Brevundimonas balnearis sp. nov., isolated from the well water of a thermal bath.Toth E, Szuroczki S, Keki Z, Kosztik J, Makk J, Boka K, Sproer C, Marialigeti K, Schumann PInt J Syst Evol Microbiol10.1099/ijsem.0.0017462017Bacterial Typing Techniques, Base Composition, Caulobacteraceae/*classification/genetics/isolation & purification, DNA, Bacterial/genetics, Fatty Acids/chemistry, Hungary, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Temperature, Ubiquinone/analogs & derivatives/chemistry, *Water Microbiology, *Water WellsTranscriptome

Reference

@idauthorscataloguedoi/urltitle
3061Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 7226)https://www.dsmz.de/collection/catalogue/details/culture/DSM-7226
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
35302Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/12565
65626Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 2032)https://www.ccug.se/strain?id=2032
68369Automatically annotated from API 20NE
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta