Strain identifier

BacDive ID: 22992

Type strain: Yes

Species: Desulfonatronum alkalitolerans

Strain Designation: HSRB-L1, HSRB-L, HTRB-L

Strain history: <- D. Y. Sorokin, Dept. Biotechnol., Delft Univ. Technol., Netherlands; HSRB-L <- D. Y. Sorokin {2009}

NCBI tax ID(s): 680375 (species)

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General

@ref: 19309

BacDive-ID: 22992

DSM-Number: 24646

keywords: 16S sequence, Bacteria, anaerobe, mesophilic

description: Desulfonatronum alkalitolerans HSRB-L1 is an anaerobe, mesophilic bacterium that was isolated from bioreactor used for the removal of hydrogen sulfide from waste gases.

NCBI tax id

  • NCBI tax id: 680375
  • Matching level: species

strain history

  • @ref: 19309
  • history: <- D. Y. Sorokin, Dept. Biotechnol., Delft Univ. Technol., Netherlands; HSRB-L <- D. Y. Sorokin {2009}

doi: 10.13145/bacdive22992.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Deltaproteobacteria
  • order: Desulfovibrionales
  • family: Desulfonatronaceae
  • genus: Desulfonatronum
  • species: Desulfonatronum alkalitolerans
  • full scientific name: Desulfonatronum alkalitolerans Sorokin et al. 2013

@ref: 19309

domain: Bacteria

phylum: Proteobacteria

class: Deltaproteobacteria

order: Desulfovibrionales

family: Desulfonatronaceae

genus: Desulfonatronum

species: Desulfonatronum alkalitolerans

full scientific name: Desulfonatronum alkalitolerans Sorokin et al. 2013

strain designation: HSRB-L1, HSRB-L, HTRB-L

type strain: yes

Morphology

colony morphology

  • @ref: 19309
  • incubation period: 8-14 days

Culture and growth conditions

culture medium

  • @ref: 19309
  • name: DESULFONATRONUM ALKALITOLERANS MEDIUM (DSMZ Medium 1485)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/1485
  • composition: Name: DESULFONATRONUM ALKALITOLERANS MEDIUM (DSMZ Medium 1485) Composition: NaHCO3 31.9361 g/l Na2CO3 10.5788 g/l NaCl 5.98802 g/l Na-formate 3.39321 g/l Na2SO4 2.79441 g/l K2HPO4 0.998002 g/l Na2S x 9 H2O 0.299401 g/l MgCl2 x 6 H2O 0.199601 g/l NH4Cl 0.199601 g/l Na-acetate 0.199601 g/l Yeast extract 0.0199601 g/l EDTA 0.00499002 g/l FeSO4 x 7 H2O 0.00219561 g/l NaOH 0.000499002 g/l CoCl2 x 6 H2O 0.000199601 g/l ZnSO4 x 7 H2O 9.98004e-05 g/l Na2MoO4 x 2 H2O 2.99401e-05 g/l H3BO3 2.99401e-05 g/l CuCl2 x 2 H2O 2.99401e-05 g/l NiCl2 x 6 H2O 2.99401e-05 g/l MnCl2 x 4 H2O 2.99401e-05 g/l Na2WO4 x 2 H2O 3.99202e-06 g/l Na2SeO3 x 5 H2O 2.99401e-06 g/l Distilled water

culture temp

  • @ref: 19309
  • growth: positive
  • type: growth
  • temperature: 30
  • range: mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 19309
  • oxygen tolerance: anaerobe

Isolation, sampling and environmental information

isolation

  • @ref: 19309
  • sample type: bioreactor used for the removal of hydrogen sulfide from waste gases
  • geographic location: Lelystad
  • country: Netherlands
  • origin.country: NLD
  • continent: Europe

isolation source categories

Cat1Cat2Cat3
#Engineered#Bioreactor
#Engineered#Waste#Waste gas
#Condition#Sulfuric

taxonmaps

  • @ref: 69479
  • File name: preview.99_185031.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_453;96_46347;97_59488;98_120403;99_185031&stattab=map
  • Last taxonomy: Desulfonatronum alkalitolerans subclade
  • 16S sequence: GQ863488
  • Sequence Identity:
  • Total samples: 118
  • soil counts: 29
  • aquatic counts: 67
  • animal counts: 18
  • plant counts: 4

Safety information

risk assessment

  • @ref: 19309
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 19309
  • description: Desulfonatronum alkalitolerans strain HSRB-L1 16S ribosomal RNA gene, partial sequence
  • accession: GQ863488
  • length: 1485
  • database: ena
  • NCBI tax ID: 680375

GC content

  • @ref: 19309
  • GC-content: 55.8
  • method: thermal denaturation, midpoint method (Tm)

External links

@ref: 19309

culture collection no.: DSM 24646, UNIQEM U799

straininfo link

  • @ref: 87238
  • straininfo: 400682

literature

  • topic: Phylogeny
  • Pubmed-ID: 23564266
  • title: Isolation and characterization of two novel alkalitolerant sulfidogens from a Thiopaq bioreactor, Desulfonatronum alkalitolerans sp. nov., and Sulfurospirillum alkalitolerans sp. nov.
  • authors: Sorokin DY, Tourova TP, Muyzer G
  • journal: Extremophiles
  • DOI: 10.1007/s00792-013-0538-4
  • year: 2013
  • mesh: Base Sequence, Bioreactors/*microbiology, DNA, Bacterial/chemistry, Deltaproteobacteria/genetics/*isolation & purification/metabolism, Epsilonproteobacteria/genetics/*isolation & purification/metabolism, Molecular Sequence Data, Phylogeny, Sequence Analysis, DNA, Sulfur/metabolism
  • topic2: Metabolism

Reference

@idauthorscataloguedoi/urltitle
19309Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 24646)https://www.dsmz.de/collection/catalogue/details/culture/DSM-24646
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
87238Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID400682.1StrainInfo: A central database for resolving microbial strain identifiers