Strain identifier
BacDive ID: 22992
Type strain:
Species: Desulfonatronum alkalitolerans
Strain Designation: HSRB-L1, HSRB-L, HTRB-L
Strain history: <- D. Y. Sorokin, Dept. Biotechnol., Delft Univ. Technol., Netherlands; HSRB-L <- D. Y. Sorokin {2009}
NCBI tax ID(s): 680375 (species)
General
@ref: 19309
BacDive-ID: 22992
DSM-Number: 24646
keywords: 16S sequence, Bacteria, anaerobe, mesophilic
description: Desulfonatronum alkalitolerans HSRB-L1 is an anaerobe, mesophilic bacterium that was isolated from bioreactor used for the removal of hydrogen sulfide from waste gases.
NCBI tax id
- NCBI tax id: 680375
- Matching level: species
strain history
- @ref: 19309
- history: <- D. Y. Sorokin, Dept. Biotechnol., Delft Univ. Technol., Netherlands; HSRB-L <- D. Y. Sorokin {2009}
doi: 10.13145/bacdive22992.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Deltaproteobacteria
- order: Desulfovibrionales
- family: Desulfonatronaceae
- genus: Desulfonatronum
- species: Desulfonatronum alkalitolerans
- full scientific name: Desulfonatronum alkalitolerans Sorokin et al. 2013
@ref: 19309
domain: Bacteria
phylum: Proteobacteria
class: Deltaproteobacteria
order: Desulfovibrionales
family: Desulfonatronaceae
genus: Desulfonatronum
species: Desulfonatronum alkalitolerans
full scientific name: Desulfonatronum alkalitolerans Sorokin et al. 2013
strain designation: HSRB-L1, HSRB-L, HTRB-L
type strain: yes
Morphology
colony morphology
- @ref: 19309
- incubation period: 8-14 days
Culture and growth conditions
culture medium
- @ref: 19309
- name: DESULFONATRONUM ALKALITOLERANS MEDIUM (DSMZ Medium 1485)
- growth: yes
- link: https://mediadive.dsmz.de/medium/1485
- composition: Name: DESULFONATRONUM ALKALITOLERANS MEDIUM (DSMZ Medium 1485) Composition: NaHCO3 31.9361 g/l Na2CO3 10.5788 g/l NaCl 5.98802 g/l Na-formate 3.39321 g/l Na2SO4 2.79441 g/l K2HPO4 0.998002 g/l Na2S x 9 H2O 0.299401 g/l MgCl2 x 6 H2O 0.199601 g/l NH4Cl 0.199601 g/l Na-acetate 0.199601 g/l Yeast extract 0.0199601 g/l EDTA 0.00499002 g/l FeSO4 x 7 H2O 0.00219561 g/l NaOH 0.000499002 g/l CoCl2 x 6 H2O 0.000199601 g/l ZnSO4 x 7 H2O 9.98004e-05 g/l Na2MoO4 x 2 H2O 2.99401e-05 g/l H3BO3 2.99401e-05 g/l CuCl2 x 2 H2O 2.99401e-05 g/l NiCl2 x 6 H2O 2.99401e-05 g/l MnCl2 x 4 H2O 2.99401e-05 g/l Na2WO4 x 2 H2O 3.99202e-06 g/l Na2SeO3 x 5 H2O 2.99401e-06 g/l Distilled water
culture temp
- @ref: 19309
- growth: positive
- type: growth
- temperature: 30
- range: mesophilic
Physiology and metabolism
oxygen tolerance
- @ref: 19309
- oxygen tolerance: anaerobe
Isolation, sampling and environmental information
isolation
- @ref: 19309
- sample type: bioreactor used for the removal of hydrogen sulfide from waste gases
- geographic location: Lelystad
- country: Netherlands
- origin.country: NLD
- continent: Europe
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Engineered | #Bioreactor | |
#Engineered | #Waste | #Waste gas |
#Condition | #Sulfuric |
taxonmaps
- @ref: 69479
- File name: preview.99_185031.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_453;96_46347;97_59488;98_120403;99_185031&stattab=map
- Last taxonomy: Desulfonatronum alkalitolerans subclade
- 16S sequence: GQ863488
- Sequence Identity:
- Total samples: 118
- soil counts: 29
- aquatic counts: 67
- animal counts: 18
- plant counts: 4
Safety information
risk assessment
- @ref: 19309
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 19309
- description: Desulfonatronum alkalitolerans strain HSRB-L1 16S ribosomal RNA gene, partial sequence
- accession: GQ863488
- length: 1485
- database: ena
- NCBI tax ID: 680375
GC content
- @ref: 19309
- GC-content: 55.8
- method: thermal denaturation, midpoint method (Tm)
External links
@ref: 19309
culture collection no.: DSM 24646, UNIQEM U799
straininfo link
- @ref: 87238
- straininfo: 400682
literature
- topic: Phylogeny
- Pubmed-ID: 23564266
- title: Isolation and characterization of two novel alkalitolerant sulfidogens from a Thiopaq bioreactor, Desulfonatronum alkalitolerans sp. nov., and Sulfurospirillum alkalitolerans sp. nov.
- authors: Sorokin DY, Tourova TP, Muyzer G
- journal: Extremophiles
- DOI: 10.1007/s00792-013-0538-4
- year: 2013
- mesh: Base Sequence, Bioreactors/*microbiology, DNA, Bacterial/chemistry, Deltaproteobacteria/genetics/*isolation & purification/metabolism, Epsilonproteobacteria/genetics/*isolation & purification/metabolism, Molecular Sequence Data, Phylogeny, Sequence Analysis, DNA, Sulfur/metabolism
- topic2: Metabolism
Reference
@id | authors | catalogue | doi/url | title |
---|---|---|---|---|
19309 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 24646) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-24646 | |
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |
87238 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID400682.1 | StrainInfo: A central database for resolving microbial strain identifiers |