Strain identifier
BacDive ID: 22990
Type strain: 
Species: Desulfatitalea tepidiphila
Strain Designation: S28bF
Strain history: <- Y. Higashioka, Hokkaido University; S28bF
NCBI tax ID(s): 1185843 (species)
version 8.1 (current version)
General
@ref: 19243
BacDive-ID: 22990
DSM-Number: 23472
keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic, Gram-negative, motile, rod-shaped
description: Desulfatitalea tepidiphila S28bF is an anaerobe, mesophilic, Gram-negative bacterium that was isolated from tidal flat sediment.
NCBI tax id
- NCBI tax id: 1185843
- Matching level: species
strain history
- @ref: 19243
- history: <- Y. Higashioka, Hokkaido University; S28bF
doi: 10.13145/bacdive22990.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Deltaproteobacteria
- order: Desulfobacterales
- family: Desulfobacteraceae
- genus: Desulfatitalea
- species: Desulfatitalea tepidiphila
- full scientific name: Desulfatitalea tepidiphila Higashioka et al. 2013
@ref: 19243
domain: Bacteria
phylum: Proteobacteria
class: Deltaproteobacteria
order: Desulfobacterales
family: Desulfobacteraceae
genus: Desulfatitalea
species: Desulfatitalea tepidiphila
full scientific name: Desulfatitalea tepidiphila Higashioka et al. 2013
strain designation: S28bF
type strain: yes
Morphology
cell morphology
- @ref: 30733
- gram stain: negative
- cell length: 2.75 µm
- cell width: 0.55 µm
- cell shape: rod-shaped
- motility: yes
colony morphology
- @ref: 19243
- incubation period: 3-7 days
Culture and growth conditions
culture medium
- @ref: 19243
- name: DESULFUROMUSA MEDIUM (DSMZ Medium 195a)
- growth: yes
- link: https://mediadive.dsmz.de/medium/195a
- composition: Name: DESULFUROMUSA MEDIUM (DSMZ Medium 195a) Composition: NaCl 20.9372 g/l Na2SO4 2.99103 g/l MgCl2 x 6 H2O 2.99103 g/l Na2-fumarate 2.49252 g/l Na2CO3 1.49551 g/l KCl 0.498504 g/l Na2S x 9 H2O 0.398804 g/l NH4Cl 0.299103 g/l KH2PO4 0.199402 g/l CaCl2 x 2 H2O 0.149551 g/l HCl 0.00249252 g/l FeCl2 x 4 H2O 0.00149551 g/l NaOH 0.000498504 g/l Sodium resazurin 0.000498504 g/l CoCl2 x 6 H2O 0.000189432 g/l Pyridoxine hydrochloride 9.97009e-05 g/l MnCl2 x 4 H2O 9.97009e-05 g/l ZnCl2 6.97906e-05 g/l p-Aminobenzoic acid 4.98504e-05 g/l (DL)-alpha-Lipoic acid 4.98504e-05 g/l Thiamine HCl 4.98504e-05 g/l Calcium D-(+)-pantothenate 4.98504e-05 g/l Riboflavin 4.98504e-05 g/l Nicotinic acid 4.98504e-05 g/l Na2MoO4 x 2 H2O 3.58923e-05 g/l NiCl2 x 6 H2O 2.39282e-05 g/l Biotin 1.99402e-05 g/l Folic acid 1.99402e-05 g/l H3BO3 5.98205e-06 g/l Na2WO4 x 2 H2O 3.98804e-06 g/l Na2SeO3 x 5 H2O 2.99103e-06 g/l CuCl2 x 2 H2O 1.99402e-06 g/l Vitamin B12 9.97009e-07 g/l Distilled water
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 19243 | positive | growth | 37 | mesophilic |
| 30733 | positive | growth | 13-45 | |
| 30733 | positive | optimum | 38 | mesophilic |
culture pH
| @ref | ability | type | pH |
|---|---|---|---|
| 30733 | positive | growth | 6.2-7.9 |
| 30733 | positive | optimum | 7.05 |
Physiology and metabolism
oxygen tolerance
| @ref | oxygen tolerance |
|---|---|
| 19243 | anaerobe |
| 30733 | anaerobe |
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 30733 | 30089 | acetate | + | carbon source |
| 30733 | 15740 | formate | + | carbon source |
| 30733 | 24996 | lactate | + | carbon source |
| 30733 | 17272 | propionate | + | carbon source |
| 30733 | 15361 | pyruvate | + | carbon source |
| 30733 | 30031 | succinate | + | carbon source |
Isolation, sampling and environmental information
isolation
- @ref: 19243
- sample type: tidal flat sediment
- geographic location: Chiba, Urayasu, Sanban-ze (39° 39.0' N, 139° 55.5' E)
- country: Japan
- origin.country: JPN
- continent: Asia
- latitude: 35.642
- longitude: 139.903
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Environmental | #Terrestrial | #Tidal flat |
| #Environmental | #Terrestrial | #Sediment |
taxonmaps
- @ref: 69479
- File name: preview.99_7074.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_127;96_3318;97_4069;98_5200;99_7074&stattab=map
- Last taxonomy: Desulfatitalea tepidiphila subclade
- 16S sequence: AB614135
- Sequence Identity:
- Total samples: 758
- soil counts: 58
- aquatic counts: 687
- animal counts: 7
- plant counts: 6
Safety information
risk assessment
- @ref: 19243
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 19243
- description: Desulfatitalea tepidiphila gene for 16S ribosomal RNA, partial sequence, strain: S28bF
- accession: AB614135
- length: 1485
- database: ena
- NCBI tax ID: 1185843
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Desulfatitalea tepidiphila S28bF | GCA_001293685 | contig | ncbi | 1185843 |
| 66792 | Desulfatitalea tepidiphila S28bF | 2690315754 | draft | img | 1185843 |
GC content
| @ref | GC-content | method |
|---|---|---|
| 19243 | 56.6 | high performance liquid chromatography (HPLC) |
| 30733 | 56.6 |
External links
@ref: 19243
culture collection no.: DSM 23472, NBRC 107166
straininfo link
- @ref: 87236
- straininfo: 402786
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 22581901 | Desulfatitalea tepidiphila gen. nov., sp. nov., a sulfate-reducing bacterium isolated from tidal flat sediment. | Higashioka Y, Kojima H, Watanabe M, Fukui M | Int J Syst Evol Microbiol | 10.1099/ijs.0.043356-0 | 2012 | Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Deltaproteobacteria/*classification/genetics/isolation & purification, Fatty Acids/analysis, Genes, Bacterial, Geologic Sediments/*microbiology, Japan, Molecular Sequence Data, *Phylogeny, RNA, Ribosomal, 16S/genetics, Seawater/*microbiology, Sequence Analysis, DNA, Sulfur-Reducing Bacteria/*classification/genetics/isolation & purification, Water Microbiology | Enzymology |
| Genetics | 26564045 | Draft Genome Sequence of Desulfatitalea tepidiphila S28bFT. | Higashioka Y, Kojima H, Watanabe T, Fukui M | Genome Announc | 10.1128/genomeA.01326-15 | 2015 |
Reference
| @id | authors | catalogue | doi/url | title | ID_cross_reference | pubmed |
|---|---|---|---|---|---|---|
| 19243 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 23472) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-23472 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 30733 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | 10.1128/mSphere.00237-17 | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 27064 | 28776041 | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
| 87236 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID402786.1 | StrainInfo: A central database for resolving microbial strain identifiers |