Strain identifier

BacDive ID: 22988

Type strain: Yes

Species: Tonsilliphilus suis

Strain Designation: W254

Strain history: S. Murakami W254.

NCBI tax ID(s): 214014 (species)

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General

@ref: 19363

BacDive-ID: 22988

DSM-Number: 21880

keywords: 16S sequence, Bacteria, anaerobe, spore-forming, mesophilic, Gram-positive, motile, coccus-shaped, animal pathogen

description: Tonsilliphilus suis W254 is an anaerobe, spore-forming, mesophilic animal pathogen that was isolated from submandibular lymph node of an infected pig.

NCBI tax id

  • NCBI tax id: 214014
  • Matching level: species

strain history

@refhistory
19363<- S. Murakami, Tokyo Univ. Agriculture, Dept. Animal Sci., Atsugi, Japan; W254 <- R. Azuma
67770S. Murakami W254.

doi: 10.13145/bacdive22988.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Micrococcales
  • family: Dermatophilaceae
  • genus: Tonsilliphilus
  • species: Tonsilliphilus suis
  • full scientific name: Tonsilliphilus suis Azuma et al. 2013

@ref: 19363

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Dermatophilaceae

genus: Tonsilliphilus

species: Tonsilliphilus suis

full scientific name: Tonsilliphilus suis Azuma et al. 2013

strain designation: W254

type strain: yes

Morphology

cell morphology

  • @ref: 30821
  • gram stain: positive
  • cell shape: coccus-shaped
  • motility: yes

colony morphology

  • @ref: 19363
  • incubation period: 3-7 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
19363PYG MEDIUM (MODIFIED) (DSMZ Medium 104)yeshttps://mediadive.dsmz.de/medium/104Name: PYG MEDIUM (modified) (DSMZ Medium 104) Composition: Yeast extract 10.0 g/l Peptone 5.0 g/l Trypticase peptone 5.0 g/l Beef extract 5.0 g/l Glucose 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l K2HPO4 0.04 g/l KH2PO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Hemin 0.005 g/l Ethanol 0.0038 g/l Resazurin 0.001 g/l Tween 80 Vitamin K1 NaOH Distilled water
19363COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base

culture temp

@refgrowthtypetemperaturerange
19363positivegrowth37mesophilic
30821positiveoptimum37mesophilic
67770positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
19363anaerobe
30821anaerobe

spore formation

  • @ref: 30821
  • spore formation: yes

observation

  • @ref: 67770
  • observation: quinones: MK-8(H4)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3082128757fructose+carbon source
3082128260galactose+carbon source
3082117234glucose+carbon source
3082117716lactose+carbon source
3082117306maltose+carbon source
3082129864mannitol+carbon source
3082137684mannose+carbon source
3082128053melibiose+carbon source
3082116634raffinose+carbon source
3082117814salicin+carbon source
3082130911sorbitol+carbon source
3082117992sucrose+carbon source
3082127082trehalose+carbon source
308214853esculin+hydrolysis
3082117632nitrate+reduction
6838016199urea-hydrolysis
6838029016arginine-hydrolysis
6838016634raffinose+fermentation
6838029985L-glutamate-degradation
6838017632nitrate+reduction
6838027897tryptophan-energy source

metabolite production

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68380serine arylamidase-
68380glutamyl-glutamate arylamidase-
68380histidine arylamidase-
68380glycin arylamidase-
68380alanine arylamidase-3.4.11.2
68380tyrosine arylamidase+
68380pyrrolidonyl arylamidase-3.4.19.3
68380leucine arylamidase+3.4.11.1
68380phenylalanine arylamidase+
68380leucyl glycin arylamidase-3.4.11.1
68380proline-arylamidase-3.4.11.5
68380L-arginine arylamidase+
68380alkaline phosphatase-3.1.3.1
68380tryptophan deaminase-4.1.99.1
68380alpha-fucosidase-3.2.1.51
68380glutamate decarboxylase-4.1.1.15
68380N-acetyl-beta-glucosaminidase-3.2.1.52
68380beta-glucuronidase-3.2.1.31
68380alpha-arabinosidase-3.2.1.55
68380beta-glucosidase+3.2.1.21
68380alpha-glucosidase+3.2.1.20
68380beta-Galactosidase 6-phosphate-
68380beta-galactosidase+3.2.1.23
68380alpha-galactosidase+3.2.1.22
68380arginine dihydrolase-3.5.3.6
68380urease-3.5.1.5

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
19363--++-++---+/-+--+--+--++-+-----

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinent
19363submandibular lymph node of an infected pigJapanJPNAsia
67770Infected swine submandibular lymph node

isolation source categories

Cat1Cat2Cat3
#Infection#Disease
#Host#Mammals#Suidae (Pig,Swine)
#Host Body-Site#Oral cavity and airways
#Host Body-Site#Organ#Lymph node

Safety information

risk assessment

  • @ref: 19363
  • pathogenicity animal: yes
  • biosafety level: 2
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 19363
  • description: Tonsilliphilus suis gene for 16S rRNA, partial sequence, strain: W254
  • accession: AB096084
  • length: 1534
  • database: ena
  • NCBI tax ID: 214014

GC content

@refGC-contentmethod
1936372.2high performance liquid chromatography (HPLC)
3082172.2

External links

@ref: 19363

culture collection no.: DSM 21880, ATCC 35846, CCM 3774, JCM 15727

straininfo link

  • @ref: 87234
  • straininfo: 66937

literature

  • topic: Phylogeny
  • Pubmed-ID: 23243095
  • title: Tonsilliphilus suis gen. nov., sp. nov., causing tonsil infections in pigs.
  • authors: Azuma R, Ung-Bok B, Murakami S, Ishiwata H, Osaki M, Shimada N, Ito Y, Miyagawa E, Makino T, Kudo T, Takahashi Y, Yano I, Murata R, Yokoyama E
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.045237-0
  • year: 2012
  • mesh: Actinomycetales/*classification/genetics/isolation & purification, Animals, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/analysis, Molecular Sequence Data, Nucleic Acid Hybridization, Palatine Tonsil/*microbiology, Phospholipids/analysis, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Swine/*microbiology, Swine Diseases/microbiology, Vitamin K 2/analogs & derivatives/analysis
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
19363Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 21880)https://www.dsmz.de/collection/catalogue/details/culture/DSM-21880
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
30821Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2715228776041
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68380Automatically annotated from API rID32A
87234Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID66937.1StrainInfo: A central database for resolving microbial strain identifiers