Strain identifier

BacDive ID: 22981

Type strain: Yes

Species: Enorma massiliensis

Strain Designation: phl

Strain history: <- P. Hugon, Timone Hospital, Marseille, France; phl

NCBI tax ID(s): 1472761 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 19353

BacDive-ID: 22981

DSM-Number: 25476

keywords: 16S sequence, Bacteria, obligate anaerobe, mesophilic

description: Enorma massiliensis phl is an obligate anaerobe, mesophilic bacterium that was isolated from human feces, 26-year-old woman suffering from morbid obesity.

NCBI tax id

  • NCBI tax id: 1472761
  • Matching level: species

strain history

  • @ref: 19353
  • history: <- P. Hugon, Timone Hospital, Marseille, France; phl

doi: 10.13145/bacdive22981.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Coriobacteriia
  • order: Coriobacteriales
  • family: Coriobacteriaceae
  • genus: Enorma
  • species: Enorma massiliensis
  • full scientific name: Enorma massiliensis Mishra et al. 2016

@ref: 19353

domain: Bacteria

phylum: Actinobacteria

class: Coriobacteriia

order: Coriobacteriales

family: Coriobacteriaceae

genus: Enorma

species: Enorma massiliensis

full scientific name: Enorma massiliensis Mishra et al. 2016

strain designation: phl

type strain: yes

Morphology

colony morphology

  • @ref: 19353
  • incubation period: 2-3 days

Culture and growth conditions

culture medium

  • @ref: 19353
  • name: CHOPPED MEAT MEDIUM (DSMZ Medium 78)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/78
  • composition: Name: CHOPPED MEAT MEDIUM (DSMZ Medium 78) Composition: Ground beef 500.0 g/l Casitone 30.0 g/l Agar 15.0 g/l Ethanol 9.5 g/l (optional) K2HPO4 5.0 g/l Yeast extract 5.0 g/l L-Cysteine HCl 0.5 g/l Haemin 0.005 g/l (optional) Resazurin 0.001 g/l Vitamin K3 0.0005 g/l (optional) Vitamin K1 (optional) NaOH (optional) Distilled water

culture temp

  • @ref: 19353
  • growth: positive
  • type: growth
  • temperature: 37
  • range: mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 19353
  • oxygen tolerance: obligate anaerobe

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6838016199urea-hydrolysis
6838029016arginine+hydrolysis
6838016024D-mannose-fermentation
6838016634raffinose-fermentation
6838029985L-glutamate+degradation
6838017632nitrate-reduction
6838027897tryptophan-energy source

metabolite production

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68380serine arylamidase-
68380glutamyl-glutamate arylamidase-
68380histidine arylamidase-
68380glycin arylamidase-
68380alanine arylamidase-3.4.11.2
68380tyrosine arylamidase-
68380pyrrolidonyl arylamidase-3.4.19.3
68380leucine arylamidase-3.4.11.1
68380phenylalanine arylamidase-
68380leucyl glycin arylamidase-3.4.11.1
68380proline-arylamidase+3.4.11.5
68380L-arginine arylamidase+
68380alkaline phosphatase-3.1.3.1
68380tryptophan deaminase-4.1.99.1
68380alpha-fucosidase-3.2.1.51
68380glutamate decarboxylase+4.1.1.15
68380N-acetyl-beta-glucosaminidase-3.2.1.52
68380beta-glucuronidase-3.2.1.31
68380alpha-arabinosidase-3.2.1.55
68380beta-glucosidase-3.2.1.21
68380beta-Galactosidase 6-phosphate-
68380beta-galactosidase-3.2.1.23
68380alpha-galactosidase+3.2.1.22
68380arginine dihydrolase+3.5.3.6
68380urease-3.5.1.5

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
19353-++--+/-------+----++----------

Isolation, sampling and environmental information

isolation

  • @ref: 19353
  • sample type: human feces, 26-year-old woman suffering from morbid obesity
  • geographic location: Marseille
  • country: France
  • origin.country: FRA
  • continent: Europe

isolation source categories

Cat1Cat2Cat3
#Host#Human#Female
#Host Body Product#Gastrointestinal tract#Feces (Stool)

taxonmaps

  • @ref: 69479
  • File name: preview.99_7288.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_147;96_3410;97_4185;98_5347;99_7288&stattab=map
  • Last taxonomy: Enorma massiliensis subclade
  • 16S sequence: JN837493
  • Sequence Identity:
  • Total samples: 5810
  • soil counts: 81
  • aquatic counts: 133
  • animal counts: 5580
  • plant counts: 16

Safety information

risk assessment

  • @ref: 19353
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 19353
  • description: Enorma massiliensis phI 16S ribosomal RNA gene, partial sequence
  • accession: JN837493
  • length: 1464
  • database: ena
  • NCBI tax ID: 1118060

GC content

  • @ref: 19353
  • GC-content: 62.0
  • method: sequence analysis

External links

@ref: 19353

culture collection no.: DSM 25476, CSUR P183

straininfo link

  • @ref: 87227
  • straininfo: 403998

Reference

@idauthorscataloguedoi/urltitle
19353Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 25476)https://www.dsmz.de/collection/catalogue/details/culture/DSM-25476
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
68380Automatically annotated from API rID32A
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
87227Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID403998.1StrainInfo: A central database for resolving microbial strain identifiers