Strain identifier
BacDive ID: 22970
Type strain: 
Species: Keratinibaculum paraultunense
Strain Designation: KD-1
Strain history: Y. Huang KD-1.
NCBI tax ID(s): 1278232 (species)
version 8.1 (current version)
General
@ref: 19350
BacDive-ID: 22970
DSM-Number: 26752
keywords: genome sequence, 16S sequence, Bacteria, anaerobe, spore-forming, thermophilic, Gram-positive
description: Keratinibaculum paraultunense KD-1 is an anaerobe, spore-forming, thermophilic bacterium that was isolated from soil from grassy marshland.
NCBI tax id
- NCBI tax id: 1278232
- Matching level: species
strain history
| @ref | history |
|---|---|
| 19350 | <- Y. Huang, Biogas Inst. of Ministry of Agriculture, Chengdu, P.R. China <- Y. Huang and Y. Sun |
| 67770 | Y. Huang KD-1. |
doi: 10.13145/bacdive22970.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Clostridia
- order: Tissierellales
- family: Tissierellaceae
- genus: Keratinibaculum
- species: Keratinibaculum paraultunense
- full scientific name: Keratinibaculum paraultunense Huang et al. 2015
@ref: 19350
domain: Bacteria
phylum: Firmicutes
class: Tissierellia
order: Clostridiales
family: Tissierellaceae
genus: Keratinibaculum
species: Keratinibaculum paraultunense
full scientific name: Keratinibaculum paraultunense Huang et al. 2015
strain designation: KD-1
type strain: yes
Morphology
cell morphology
- @ref: 69480
- gram stain: positive
- confidence: 99.719
Culture and growth conditions
culture medium
- @ref: 19350
- name: PY + X MEDIUM (DSMZ Medium 104b)
- growth: yes
- link: https://mediadive.dsmz.de/medium/104b
- composition: Name: PY + X MEDIUM (DSMZ Medium 104b) Composition: Yeast extract 10.0 g/l D-Glucose 5.0 g/l Trypticase peptone 5.0 g/l Meat peptone 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l KH2PO4 0.04 g/l K2HPO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Sodium resazurin 0.0005 g/l Distilled water
culture temp
| @ref | growth | type | temperature | range | confidence |
|---|---|---|---|---|---|
| 19350 | positive | growth | 55 | thermophilic | |
| 67770 | positive | growth | 55 | thermophilic | |
| 69480 | thermophilic | 99.649 |
Physiology and metabolism
oxygen tolerance
| @ref | oxygen tolerance | confidence |
|---|---|---|
| 19350 | anaerobe | |
| 69480 | anaerobe | 100 |
spore formation
| @ref | spore formation | confidence |
|---|---|---|
| 69481 | yes | 95 |
| 69480 | yes | 99.447 |
Isolation, sampling and environmental information
isolation
| @ref | sample type | country | origin.country | continent |
|---|---|---|---|---|
| 19350 | soil from grassy marshland | Finland | FIN | Europe |
| 67770 | Grassy marshland |
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Environmental | #Terrestrial | #Grassland |
| #Environmental | #Terrestrial | #Soil |
taxonmaps
- @ref: 69479
- File name: preview.99_140664.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_207;96_35336;97_44853;98_93490;99_140664&stattab=map
- Last taxonomy: Keratinibaculum paraultunense subclade
- 16S sequence: KC188660
- Sequence Identity:
- Total samples: 179
- soil counts: 13
- aquatic counts: 96
- animal counts: 64
- plant counts: 6
Safety information
risk assessment
- @ref: 19350
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 19350
- description: Keratinibaculum paraultunense strain KD-1 16S ribosomal RNA gene, partial sequence
- accession: KC188660
- length: 1491
- database: ena
- NCBI tax ID: 1278232
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Keratinibaculum paraultunense KD-1 | GCA_016767175 | complete | ncbi | 1278232 |
| 66792 | Keratinibaculum paraultunense strain DSM 26752 | 1278232.3 | wgs | patric | 1278232 |
| 66792 | Keratinibaculum paraultunense DSM 26752 | 2784746792 | draft | img | 1278232 |
| 67770 | Keratinibaculum paraultunense DSM 26752 | GCA_004343355 | scaffold | ncbi | 1278232 |
GC content
- @ref: 19350
- GC-content: 28
- method: thermal denaturation, midpoint method (Tm)
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| spore-forming | yes | 95 | no |
| flagellated | no | 83.509 | no |
| gram-positive | yes | 74.675 | no |
| anaerobic | yes | 99.257 | yes |
| aerobic | no | 97.333 | yes |
| halophile | no | 89.055 | no |
| spore-forming | yes | 86.18 | no |
| thermophile | yes | 80.217 | no |
| glucose-util | yes | 82.986 | no |
| motile | yes | 84.344 | no |
| glucose-ferment | no | 50 | no |
External links
@ref: 19350
culture collection no.: DSM 26752, JCM 18769
straininfo link
- @ref: 87217
- straininfo: 397748
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 23710623 | Isolation and characterization of Keratinibaculum paraultunense gen. nov., sp. nov., a novel thermophilic, anaerobic bacterium with keratinolytic activity. | Huang Y, Sun Y, Ma S, Chen L, Zhang H, Deng Y | FEMS Microbiol Lett | 10.1111/1574-6968.12184 | 2013 | Animals, Bacteria, Anaerobic/classification/*enzymology/genetics/*isolation & purification, Bacterial Proteins/chemistry/genetics/*metabolism, Chickens, Enzyme Stability, Feathers/metabolism, Gram-Positive Bacteria/classification/genetics/*isolation & purification/*metabolism, Hot Temperature, Keratins/*metabolism, Molecular Sequence Data, Peptide Hydrolases/chemistry/genetics/*metabolism, Phylogeny, *Soil Microbiology | Enzymology |
| Metabolism | 36127480 | Genome-wide analysis of Keratinibaculum paraultunense strain KD-1( T) and its key genes and metabolic pathways involved in the anaerobic degradation of feather keratin. | Wu W, Ma S, Chen R, Huang Y, Deng Y | Arch Microbiol | 10.1007/s00203-022-03226-9 | 2022 | Amino Acids/metabolism, Anaerobiosis, Animals, Carbohydrates, Clostridiaceae, Disulfides/metabolism, *Feathers/metabolism, *Keratins/genetics/metabolism, Metabolic Networks and Pathways/genetics, Oxidoreductases/metabolism, Thioredoxins/metabolism | Enzymology |
Reference
| @id | authors | catalogue | doi/url | title | journal | pubmed |
|---|---|---|---|---|---|---|
| 19350 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 26752) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-26752 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 66794 | Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg | 10.1093/nar/gkaa1025 | BRENDA, the ELIXIR core data resource in 2021: new developments and updates | Nucleic Acids Res. 49: D498-D508 2020 | 33211880 | |
| 67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |||
| 87217 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID397748.1 | StrainInfo: A central database for resolving microbial strain identifiers |