Strain identifier
BacDive ID: 22969
Type strain: 
Species: Fonticella tunisiensis
Strain Designation: BELH25
Strain history: M.-L. Fardeau BELH25.
NCBI tax ID(s): 1096341 (species)
version 9.3 (current version)
General
@ref: 19343
BacDive-ID: 22969
DSM-Number: 24455
keywords: genome sequence, 16S sequence, Bacteria, anaerobe, thermophilic, Gram-positive, rod-shaped
description: Fonticella tunisiensis BELH25 is an anaerobe, thermophilic, Gram-positive bacterium that was isolated from water of a hot spring.
NCBI tax id
- NCBI tax id: 1096341
- Matching level: species
strain history
| @ref | history |
|---|---|
| 19343 | <- M. L. Fardeau, Lab. Microbiol. de l'IRD/ESIL, Univ. de Provence et de la Méditerranée, Marseille, France; BELH25 <- B. Fraj {2008} |
| 67770 | M.-L. Fardeau BELH25. |
doi: 10.13145/bacdive22969.20250331.9.3
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Clostridia
- order: Eubacteriales
- family: Clostridiaceae
- genus: Fonticella
- species: Fonticella tunisiensis
- full scientific name: Fonticella tunisiensis Fraj et al. 2013
@ref: 19343
domain: Bacteria
phylum: Firmicutes
class: Clostridia
order: Clostridiales
family: Clostridiaceae
genus: Fonticella
species: Fonticella tunisiensis
full scientific name: Fonticella tunisiensis Fraj et al. 2013
strain designation: BELH25
type strain: yes
Morphology
cell morphology
- @ref: 30684
- gram stain: positive
- cell length: 4 µm
- cell width: 0.5 µm
- cell shape: rod-shaped
- motility: no
pigmentation
- @ref: 30684
- production: yes
Culture and growth conditions
culture medium
- @ref: 19343
- name: CALDICELLULOSIRUPTOR MEDIUM (DSMZ Medium 640)
- growth: yes
- link: https://mediadive.dsmz.de/medium/640
- composition: Name: CALDICELLULOSIRUPTOR MEDIUM (DSMZ Medium 640; with strain-specific modifications) Composition: D-Glucose 2.0 g/l Trypticase peptone 2.0 g/l K2HPO4 1.5 g/l Yeast extract 1.0 g/l NH4Cl 0.9 g/l NaCl 0.9 g/l KH2PO4 0.75 g/l L-Cysteine HCl x H2O 0.75 g/l MgCl2 x 6 H2O 0.4 g/l HCl 0.0025 g/l FeCl3 x 6 H2O 0.0025 g/l FeCl2 x 4 H2O 0.0015 g/l Sodium resazurin 0.0005 g/l CoCl2 x 6 H2O 0.00019 g/l MnCl2 x 4 H2O 0.0001 g/l ZnCl2 7e-05 g/l Na2MoO4 x 2 H2O 3.6e-05 g/l NiCl2 x 6 H2O 2.4e-05 g/l H3BO3 6e-06 g/l CuCl2 x 2 H2O 2e-06 g/l Distilled water
culture temp
| @ref | growth | type | temperature |
|---|---|---|---|
| 19343 | positive | growth | 55 |
| 30684 | positive | growth | 45-70 |
| 30684 | positive | optimum | 55 |
| 67770 | positive | growth | 55 |
culture pH
| @ref | ability | type | pH |
|---|---|---|---|
| 30684 | positive | growth | 6.2-8 |
| 30684 | positive | optimum | 7 |
Physiology and metabolism
oxygen tolerance
| @ref | oxygen tolerance | confidence |
|---|---|---|
| 19343 | anaerobe | |
| 30684 | anaerobe | |
| 125439 | anaerobe | 99.5 |
spore formation
- @ref: 30684
- spore formation: no
halophily
| @ref | salt | growth | tested relation | concentration |
|---|---|---|---|---|
| 30684 | NaCl | positive | growth | 0-4 % |
| 30684 | NaCl | positive | optimum | 1 % |
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 30684 | 17057 | cellobiose | + | carbon source |
| 30684 | 28260 | galactose | + | carbon source |
| 30684 | 37684 | mannose | + | carbon source |
Isolation, sampling and environmental information
isolation
| @ref | sample type | geographic location | country | origin.country | continent |
|---|---|---|---|---|---|
| 19343 | water of a hot spring | Hammam Bourguiba near Bizerte | Tunisia | TUN | Africa |
| 67770 | Hot spring in Tunisia |
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Environmental | #Aquatic | #Thermal spring |
| #Condition | #Thermophilic (>45°C) |
taxonmaps
- @ref: 69479
- File name: preview.99_127680.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15852;96_49855;97_64191;98_85606;99_127680&stattab=map
- Last taxonomy: Fonticella tunisiensis subclade
- 16S sequence: HE604099
- Sequence Identity:
- Total samples: 2504
- soil counts: 455
- aquatic counts: 534
- animal counts: 1405
- plant counts: 110
Safety information
risk assessment
- @ref: 19343
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 19343
- description: Fonticella tunisiensis BelH25 partial 16S rRNA gene, type strain BELH25T
- accession: HE604099
- length: 1503
- database: nuccore
- NCBI tax ID: 1096341
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Fonticella tunisiensis strain DSM 24455 | 1096341.3 | wgs | patric | 1096341 |
| 66792 | Fonticella tunisiensis DSM 24455 | 2784746797 | draft | img | 1096341 |
| 67770 | Fonticella tunisiensis DSM 24455 | GCA_004364155 | scaffold | ncbi | 1096341 |
GC content
| @ref | GC-content | method |
|---|---|---|
| 19343 | 37.2 | high performance liquid chromatography (HPLC) |
| 30684 | 37.2 |
Genome-based predictions
predictions
| @ref | model | trait | description | prediction | confidence | training_data |
|---|---|---|---|---|---|---|
| 125438 | gram-positive | gram-positive | Positive reaction to Gram-staining | yes | 70.402 | yes |
| 125438 | anaerobic | anaerobic | Ability to grow under anoxygenic conditions (including facultative anaerobes) | yes | 87.698 | no |
| 125438 | spore-forming | spore-forming | Ability to form endo- or exospores | yes | 68.418 | no |
| 125438 | aerobic | aerobic | Ability to grow under oxygenic conditions (including facultative aerobes) | no | 94.991 | yes |
| 125438 | thermophile | thermophilic | Ability to grow at temperatures above or equal to 45°C | yes | 54.981 | yes |
| 125438 | motile2+ | flagellated | Ability to perform flagellated movement | yes | 74.92 | no |
| 125439 | BacteriaNet | spore_formation | Ability to form endo- or exospores | yes | 83.6 | |
| 125439 | BacteriaNet | motility | Ability to perform movement | yes | 82.8 | |
| 125439 | BacteriaNet | gram_stain | Reaction to gram-staining | variable | 83.9 | |
| 125439 | BacteriaNet | oxygen_tolerance | Oxygenic conditions needed for growth | anaerobe | 99.5 |
External links
@ref: 19343
culture collection no.: DSM 24455, JCM 17559
straininfo link
- @ref: 87216
- straininfo: 399499
literature
- topic: Phylogeny
- Pubmed-ID: 23024143
- title: Fonticella tunisiensis gen. nov., sp. nov., isolated from a hot spring.
- authors: Fraj B, Ben Hania W, Postec A, Hamdi M, Ollivier B, Fardeau ML
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijs.0.041947-0
- year: 2012
- mesh: Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/analysis, Gram-Positive Asporogenous Rods/*classification/genetics/isolation & purification, Hot Springs/*microbiology, Molecular Sequence Data, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Tunisia, Water Microbiology
- topic2: Genetics
Reference
| @id | authors | catalogue | doi/url | title | ID_cross_reference | pubmed | journal |
|---|---|---|---|---|---|---|---|
| 19343 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 24455) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-24455 | ||||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | ||||
| 30684 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | 10.1128/mSphere.00237-17 | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 27015 | 28776041 | ||
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | ||||
| 66794 | Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg | 10.1093/nar/gkaa1025 | BRENDA, the ELIXIR core data resource in 2021: new developments and updates | 33211880 | Nucleic Acids Res. 49: D498-D508 2020 | ||
| 67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | |||||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | ||||
| 87216 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID399499.1 | StrainInfo: A central database for resolving microbial strain identifiers | ||||
| 125438 | Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann | 10.1101/2024.08.12.607695 | Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets | ||||
| 125439 | Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy | https://github.com/GenomeNet/deepG | deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 |