Strain identifier

BacDive ID: 22967

Type strain: Yes

Species: Brassicibacter mesophilus

Strain Designation: BM

Strain history: W.-W. Zhang strain BM.

NCBI tax ID(s): 745119 (species)

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General

@ref: 19191

BacDive-ID: 22967

DSM-Number: 24659

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic, Gram-negative, motile, rod-shaped

description: Brassicibacter mesophilus BM is an anaerobe, mesophilic, Gram-negative bacterium that was isolated from food industry wastewater with high salinity.

NCBI tax id

  • NCBI tax id: 745119
  • Matching level: species

strain history

@refhistory
19191<- M.-X. Fang, Zhejiang Univ., Hangzhou, China; BM <- M.-X. Fang {2010}
67770W.-W. Zhang strain BM.

doi: 10.13145/bacdive22967.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Eubacteriales
  • family: Clostridiaceae
  • genus: Brassicibacter
  • species: Brassicibacter mesophilus
  • full scientific name: Brassicibacter mesophilus Fang et al. 2012

@ref: 19191

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Clostridiales

family: Clostridiaceae

genus: Brassicibacter

species: Brassicibacter mesophilus

full scientific name: Brassicibacter mesophilus Fang et al. 2012

strain designation: BM

type strain: yes

Morphology

cell morphology

  • @ref: 30378
  • gram stain: negative
  • cell length: 4.4 µm
  • cell width: 0.45 µm
  • cell shape: rod-shaped
  • motility: yes

Culture and growth conditions

culture medium

  • @ref: 19191
  • name: PY + X MEDIUM (DSMZ Medium 104b)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/104b
  • composition: Name: PY + X MEDIUM (DSMZ Medium 104b) Composition: Yeast extract 10.0 g/l D-Glucose 5.0 g/l Trypticase peptone 5.0 g/l Meat peptone 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l KH2PO4 0.04 g/l K2HPO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Sodium resazurin 0.0005 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
19191positivegrowth37mesophilic
30378positivegrowth16-44
30378positiveoptimum37mesophilic
67770positivegrowth32mesophilic

culture pH

@refabilitytypepHPH range
30378positivegrowth06-09alkaliphile
30378positiveoptimum7.5

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
19191anaerobe
30378anaerobe

spore formation

  • @ref: 30378
  • spore formation: no

halophily

@refsaltgrowthtested relationconcentration
30378NaClpositivegrowth0-8 %
30378NaClpositiveoptimum1.5 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3037828757fructose+carbon source
3037817234glucose+carbon source
3037829987glutamate+carbon source
3037815428glycine+carbon source
3037815361pyruvate+carbon source
3037833942ribose+carbon source
3037817992sucrose+carbon source

metabolite production

@refChebi-IDmetaboliteproduction
3037816136hydrogen sulfideyes
3037835581indoleyes

metabolite tests

  • @ref: 30378
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: +

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
19191food industry wastewater with high salinityZhejiang Province, CixiChinaCHNAsia
67770Food industry wastewater

isolation source categories

Cat1Cat2Cat3
#Engineered#Food production
#Engineered#Waste#Industrial wastewater
#Condition#Saline

taxonmaps

  • @ref: 69479
  • File name: preview.99_49653.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_486;96_3267;97_4006;98_5110;99_49653&stattab=map
  • Last taxonomy: Brassicibacter mesophilus subclade
  • 16S sequence: GU645013
  • Sequence Identity:
  • Total samples: 147
  • soil counts: 14
  • aquatic counts: 112
  • animal counts: 17
  • plant counts: 4

Safety information

risk assessment

  • @ref: 19191
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 19191
  • description: Brassicibacter mesophilus strain BM 16S ribosomal RNA gene, partial sequence
  • accession: GU645013
  • length: 1454
  • database: ena
  • NCBI tax ID: 745119

Genome sequences

  • @ref: 66792
  • description: Brassicibacter mesophilus DSM 24659
  • accession: 2861672352
  • assembly level: draft
  • database: img
  • NCBI tax ID: 745119

GC content

  • @ref: 19191
  • GC-content: 28.2

External links

@ref: 19191

culture collection no.: DSM 24659, JCM 16868

straininfo link

  • @ref: 87214
  • straininfo: 403317

literature

  • topic: Phylogeny
  • Pubmed-ID: 22307504
  • title: Brassicibacter mesophilus gen. nov., sp. nov., a strictly anaerobic bacterium isolated from food industry wastewater.
  • authors: Fang MX, Zhang WW, Zhang YZ, Tan HQ, Zhang XQ, Wu M, Zhu XF
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.034660-0
  • year: 2012
  • mesh: Bacteria, Anaerobic/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/analysis, Fermentation, *Food-Processing Industry, Molecular Sequence Data, Phospholipids/analysis, *Phylogeny, Quinones/analysis, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Waste Water/*microbiology
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
19191Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 24659)https://www.dsmz.de/collection/catalogue/details/culture/DSM-24659
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
30378Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2671728776041
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
87214Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID403317.1StrainInfo: A central database for resolving microbial strain identifiers