Strain identifier

BacDive ID: 22966

Type strain: No

Species: Acetivibrio thermocellus

Strain Designation: AD2

Strain history: <- R. Lamed, Tel-Aviv Univ., Dept. of Mol. Microbiol. and Biotechnol., Fac. of Life Scs., Israel; AD2 <- R. Lamed {1980}

NCBI tax ID(s): 1515 (species)

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General

@ref: 18806

BacDive-ID: 22966

DSM-Number: 25992

keywords: genome sequence, Bacteria, anaerobe, thermophilic

description: Acetivibrio thermocellus AD2 is an anaerobe, thermophilic bacterium of the family Oscillospiraceae.

NCBI tax id

  • NCBI tax id: 1515
  • Matching level: species

strain history

  • @ref: 18806
  • history: <- R. Lamed, Tel-Aviv Univ., Dept. of Mol. Microbiol. and Biotechnol., Fac. of Life Scs., Israel; AD2 <- R. Lamed {1980}

doi: 10.13145/bacdive22966.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Eubacteriales
  • family: Oscillospiraceae
  • genus: Acetivibrio
  • species: Acetivibrio thermocellus
  • full scientific name: Acetivibrio thermocellus (Viljoen et al. 1926) Tindall 2019
  • synonyms

    @refsynonym
    20215Ruminiclostridium thermocellum
    20215Clostridium thermocellum
    20215Hungateiclostridium thermocellum

@ref: 18806

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Clostridiales

family: Oscillospiraceae

genus: Acetivibrio

species: Acetivibrio thermocellus

full scientific name: Acetivibrio thermocellus (Viljoen et al. 1926) Tindall 2019

strain designation: AD2

type strain: no

Culture and growth conditions

culture medium

  • @ref: 18806
  • name: ACETIVIBRIO MEDIUM (DSMZ Medium 122)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/122
  • composition: Name: ACETIVIBRIO MEDIUM (DSMZ Medium 122) Composition: Cellulose 10.0 g/l (optional) Na2-ß-glycerophosphate x 5 H2O 6.0 g/l K2HPO4 5.5 g/l Cellobiose 5.0 g/l Yeast extract 4.5 g/l MgCl2 x 6 H2O 2.6 g/l KH2PO4 1.43 g/l (NH4)2SO4 1.3 g/l L-Glutathione 0.25 g/l CaCl2 x 2 H2O 0.13 g/l FeSO4 x 7 H2O 0.0011 g/l Sodium resazurin 0.0005 g/l Distilled water

culture temp

  • @ref: 18806
  • growth: positive
  • type: growth
  • temperature: 60
  • range: thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
18806anaerobe
43429anaerobe

observation

  • @ref: 43429
  • observation: adherance to cellulose after growth on cellobiose medium

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4342985146carboxymethylcellulose+degradation
4342962968cellulose+degradation

Isolation, sampling and environmental information

isolation

  • @ref: 18806
  • country: USA
  • origin.country: USA
  • continent: North America

Safety information

risk assessment

  • @ref: 18806
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Acetivibrio thermocellus AD2GCA_000255615completencbi1138384
66792Acetivibrio thermocellus AD2GCA_002564085contigncbi1138384

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileyes51.884no
motileyes51.884no
flagellatedno70.969no
flagellatedno70.969no
gram-positiveyes52.059no
gram-positiveyes52.059no
anaerobicyes99.999yes
anaerobicyes99.999yes
aerobicno99.111no
aerobicno99.111no
halophileno94.272no
halophileno94.272no
spore-formingyes94.781no
spore-formingyes94.781no
glucose-utilyes84.249no
glucose-utilyes84.249no
thermophileyes99.681yes
thermophileyes99.681yes
glucose-fermentyes57.683no
glucose-fermentyes57.683no

External links

@ref: 18806

culture collection no.: DSM 25992

straininfo link

  • @ref: 87213
  • straininfo: 399427

Reference

@idauthorscataloguedoi/urltitlejournal
18806Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 25992)https://www.dsmz.de/collection/catalogue/details/culture/DSM-25992
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
43429Edward A. Bayer, Rina Kenig, Raphael Lamedhttp://jb.asm.org/content/156/2/818.full.pdf+html Adherence of Clostridium thermocellum to CelluloseJ. Bacteriol. 156: 818-827 1983
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
87213Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID399427.1StrainInfo: A central database for resolving microbial strain identifiers