Strain identifier

BacDive ID: 22930

Type strain: Yes

Species: Geobacillus subterraneus subsp. aromaticivorans

Strain Designation: GE1

Strain history: CIP <- 2011, K. Guven, Science Faculty, Dicle Univ, Diyarbakir, Turkey: strain Ge1 <- K. Guven and H. Pirinccioglu

NCBI tax ID(s): 1117704 (subspecies)

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General

@ref: 19276

BacDive-ID: 22930

DSM-Number: 23066

keywords: 16S sequence, Bacteria, obligate aerobe, thermophilic, Gram-positive, rod-shaped

description: Geobacillus subterraneus subsp. aromaticivorans GE1 is an obligate aerobe, thermophilic, Gram-positive bacterium that was isolated from mud of hot spring.

NCBI tax id

  • NCBI tax id: 1117704
  • Matching level: subspecies

strain history

@refhistory
19276<- K. Guven, Faculty Sci., Dicle Univ., Diyarbakir, Turkey; GE1 <- K. Guven and H. Pirinccioglu
123354CIP <- 2011, K. Guven, Science Faculty, Dicle Univ, Diyarbakir, Turkey: strain Ge1 <- K. Guven and H. Pirinccioglu

doi: 10.13145/bacdive22930.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Geobacillus
  • species: Geobacillus subterraneus subsp. aromaticivorans
  • full scientific name: Geobacillus subterraneus subsp. aromaticivorans Poli et al. 2013

@ref: 19276

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Bacillaceae

genus: Geobacillus

species: Geobacillus subterraneus subsp. aromaticivorans

full scientific name: Geobacillus subterraneus subsp. aromaticivorans Poli et al. 2013

strain designation: GE1

type strain: yes

Morphology

cell morphology

  • @ref: 123354
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: no

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
37321MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
19276CASO AGAR (MERCK 105458) (DSMZ Medium 220)yesName: CASO AGAR (Merck 105458) (DSMZ Medium 220) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Distilled waterhttps://mediadive.dsmz.de/medium/220
19276TRYPTONE SOYA BROTH (TSB) (DSMZ Medium 545)yesName: TRYPTONE SOYA BROTH (TSB) (DSMZ Medium 545) Composition: Casein peptone 17.0 g/l NaCl 5.0 g/l Soy peptone 3.0 g/l D(+)-Glucose 2.5 g/l K2HPO4 2.5 g/l Distilled waterhttps://mediadive.dsmz.de/medium/545
123354CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
19276positivegrowth60thermophilic
37321positivegrowth55thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 123354
  • oxygen tolerance: obligate aerobe

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose+builds acid from32528
68371gentiobiose+builds acid from28066
68371xylitol-builds acid from17151
68371glycogen+builds acid from28087
68371starch+builds acid from28017
68371raffinose+builds acid from16634
68371melezitose+builds acid from6731
68371inulin-builds acid from15443
68371trehalose+builds acid from27082
68371sucrose+builds acid from17992
68371melibiose+builds acid from28053
68371lactose+builds acid from17716
68371maltose+builds acid from17306
68371cellobiose+builds acid from17057
68371salicin+builds acid from17814
68371esculin+builds acid from4853
68371arbutin+builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol+builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose+builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose+builds acid from65327
68371D-ribose+builds acid from16988
68371L-arabinose+builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol+builds acid from17754
123354nitrate+reduction17632
123354nitrite+reduction16301

metabolite production

  • @ref: 123354
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
123354oxidase+
123354catalase+1.11.1.6
123354urease+3.5.1.5
68382alkaline phosphatase-3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)-
68382lipase (C 14)-
68382leucine arylamidase-3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase-3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase+3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase+3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
123354--+--------++-++----

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
123354+--+++---++++----+--+/-+-+++++++++-++++-++---+/------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation date
19276mud of hot springSirnak, Guclukonak hot springTurkeyTURAsia
123354Environment, Mud of hot springSirnak, South EastTurkeyTURAsia2009

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Mud (Sludge)
#Environmental#Aquatic#Thermal spring
#Condition#Thermophilic (>45°C)

taxonmaps

  • @ref: 69479
  • File name: preview.99_272.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_79;96_191;97_207;98_231;99_272&stattab=map
  • Last taxonomy: Geobacillus
  • 16S sequence: HE613733
  • Sequence Identity:
  • Total samples: 8290
  • soil counts: 2618
  • aquatic counts: 1277
  • animal counts: 3638
  • plant counts: 757

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
192761Risk group (German classification)
1233541Risk group (French classification)

Sequence information

16S sequences

  • @ref: 19276
  • description: Geobacillus subterraneus subsp. aromaticivorans 16S rRNA gene, type strain Ge1T
  • accession: HE613733
  • length: 1546
  • database: ena
  • NCBI tax ID: 1117704

GC content

  • @ref: 19276
  • GC-content: 52.0
  • method: fluorimetric

External links

@ref: 19276

culture collection no.: DSM 23066, CIP 110341, CIP 110070

straininfo link

  • @ref: 87178
  • straininfo: 388309

literature

  • topic: Phylogeny
  • Pubmed-ID: 23337579
  • title: Geobacillus subterraneus subsp. aromaticivorans subsp. nov., a novel thermophilic and alkaliphilic bacterium isolated from a hot spring in Sirnak, Turkey.
  • authors: Poli A, Guven K, Romano I, Pirinccioglu H, Guven RG, Euzeby JP, Matpan F, Acer O, Orlando P, Nicolaus B
  • journal: J Gen Appl Microbiol
  • DOI: 10.2323/jgam.58.437
  • year: 2012
  • mesh: Bacterial Typing Techniques, Base Composition, DNA, Bacterial/analysis/genetics, DNA, Ribosomal/analysis/genetics, Fatty Acids, Genes, rRNA, Genotype, Geobacillus/*classification/genetics/*isolation & purification/physiology, Hot Springs/*microbiology, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Species Specificity, Turkey
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitle
19276Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 23066)https://www.dsmz.de/collection/catalogue/details/culture/DSM-23066
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
37321Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/8189
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
87178Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID388309.1StrainInfo: A central database for resolving microbial strain identifiers
123354Curators of the CIPCollection of Institut Pasteur (CIP 110341)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20110341