Strain identifier
BacDive ID: 22922
Type strain: 
Species: Cytobacillus eiseniae
Strain Designation: A1-2
Strain history: K. S. Chung A1-2.
NCBI tax ID(s): 762947 (species)
version 9.3 (current version)
General
@ref: 19165
BacDive-ID: 22922
DSM-Number: 26675
keywords: genome sequence, 16S sequence, Bacteria, spore-forming, mesophilic
description: Cytobacillus eiseniae A1-2 is a spore-forming, mesophilic bacterium that was isolated from intestinal tract of an earthworm .
NCBI tax id
- NCBI tax id: 762947
- Matching level: species
strain history
| @ref | history |
|---|---|
| 19165 | <- JCM <- K. S. Chung; A1-2 |
| 67770 | K. S. Chung A1-2. |
doi: 10.13145/bacdive22922.20250331.9.3
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Bacilli
- order: Caryophanales
- family: Bacillaceae
- genus: Cytobacillus
- species: Cytobacillus eiseniae
- full scientific name: Cytobacillus eiseniae (Hong et al. 2012) Patel and Gupta 2020
synonyms
- @ref: 20215
- synonym: Bacillus eiseniae
@ref: 19165
domain: Bacteria
phylum: Firmicutes
class: Bacilli
order: Bacillales
family: Bacillaceae
genus: Cytobacillus
species: Cytobacillus eiseniae
full scientific name: Cytobacillus eiseniae (Hong et al. 2012) Patel and Gupta 2020
strain designation: A1-2
type strain: yes
Culture and growth conditions
culture medium
- @ref: 19165
- name: CASO AGAR (MERCK 105458) (DSMZ Medium 220)
- growth: yes
- link: https://mediadive.dsmz.de/medium/220
- composition: Name: CASO AGAR (Merck 105458) (DSMZ Medium 220) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Distilled water
culture temp
| @ref | growth | type | temperature |
|---|---|---|---|
| 19165 | positive | growth | 30 |
| 67770 | positive | growth | 30 |
Physiology and metabolism
oxygen tolerance
- @ref: 125439
- oxygen tolerance: obligate aerobe
- confidence: 94.7
spore formation
| @ref | spore formation | confidence |
|---|---|---|
| 125439 | yes | 90.2 |
| 125438 | yes | 90.509 |
observation
- @ref: 67770
- observation: quinones: MK-7
Isolation, sampling and environmental information
isolation
| @ref | sample type | host species | country | origin.country | continent |
|---|---|---|---|---|---|
| 19165 | intestinal tract of an earthworm (Eisenia fetida L.) | Eisenia fetida | Republic of Korea | KOR | Asia |
| 67770 | Intestinal tract of an earthworm (Eisenia fetida L.) | Eisenia fetida L. | Republic of Korea | KOR | Asia |
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Host | #Invertebrates (Other) | #Annelida |
| #Host Body-Site | #Gastrointestinal tract |
taxonmaps
- @ref: 69479
- File name: preview.99_4689.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_3;96_264;97_291;98_3515;99_4689&stattab=map
- Last taxonomy: Cytobacillus
- 16S sequence: HM035089
- Sequence Identity:
- Total samples: 604
- soil counts: 304
- aquatic counts: 66
- animal counts: 148
- plant counts: 86
Safety information
risk assessment
- @ref: 19165
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 19165
- description: Bacillus eiseniae strain A1-2 16S ribosomal RNA gene, partial sequence
- accession: HM035089
- length: 1519
- database: nuccore
- NCBI tax ID: 762947
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Cytobacillus eiseniae DSM 26675 | GCA_017874625 | contig | ncbi | 762947 |
| 66792 | Cytobacillus eiseniae strain DSM 26675 | 762947.7 | wgs | patric | 762947 |
| 66792 | Cytobacillus eiseniae DSM 26675 | 2913480993 | draft | img | 762947 |
GC content
| @ref | GC-content | method |
|---|---|---|
| 19165 | 38.5 | |
| 67770 | 38.5 | high performance liquid chromatography (HPLC) |
Genome-based predictions
predictions
| @ref | model | trait | description | prediction | confidence | training_data |
|---|---|---|---|---|---|---|
| 125438 | gram-positive | gram-positive | Positive reaction to Gram-staining | yes | 74.241 | no |
| 125438 | anaerobic | anaerobic | Ability to grow under anoxygenic conditions (including facultative anaerobes) | no | 93.125 | no |
| 125438 | aerobic | aerobic | Ability to grow under oxygenic conditions (including facultative aerobes) | yes | 74.464 | no |
| 125438 | spore-forming | spore-forming | Ability to form endo- or exospores | yes | 90.509 | no |
| 125438 | thermophile | thermophilic | Ability to grow at temperatures above or equal to 45°C | no | 86.801 | yes |
| 125438 | motile2+ | flagellated | Ability to perform flagellated movement | yes | 80.639 | no |
| 125439 | BacteriaNet | spore_formation | Ability to form endo- or exospores | yes | 90.2 | |
| 125439 | BacteriaNet | motility | Ability to perform movement | yes | 85.4 | |
| 125439 | BacteriaNet | gram_stain | Reaction to gram-staining | variable | 82.5 | |
| 125439 | BacteriaNet | oxygen_tolerance | Oxygenic conditions needed for growth | obligate aerobe | 94.7 |
External links
@ref: 19165
culture collection no.: DSM 26675, JCM 16993, KCCM 90092
straininfo link
- @ref: 87170
- straininfo: 400715
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 22021583 | Bacillus eiseniae sp. nov., a swarming, moderately halotolerant bacterium isolated from the intestinal tract of an earthworm (Eisenia fetida L.). | Hong SW, Park JM, Kim SJ, Chung KS | Int J Syst Evol Microbiol | 10.1099/ijs.0.034892-0 | 2011 | Animals, Bacillus/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/analysis, Intestines/microbiology, Molecular Sequence Data, Oligochaeta/*microbiology, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/analogs & derivatives/analysis | Genetics |
| Phylogeny | 26289817 | Bacillus solani sp. nov., isolated from rhizosphere soil of a potato field. | Liu B, Liu GH, Sengonca C, Schumann P, Ge CB, Wang JP, Cui WD, Lin NQ | Int J Syst Evol Microbiol | 10.1099/ijsem.0.000539 | 2015 | Bacillus/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, China, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, *Rhizosphere, Sequence Analysis, DNA, *Soil Microbiology, Solanum tuberosum/*microbiology, Vitamin K 2/analogs & derivatives/chemistry | Transcriptome |
| Phylogeny | 28820088 | Bacillus praedii sp. nov., isolated from purplish paddy soil. | Liu B, Liu GH, Sengonca C, Schumann P, Wang JP, Zhu YJ, Liu QY, Wang MK | Int J Syst Evol Microbiol | 10.1099/ijsem.0.002030 | 2017 | Bacillus/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, China, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Nucleic Acid Hybridization, Oryza, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology | Transcriptome |
| Phylogeny | 28984568 | Bacillus ciccensis sp. nov., isolated from maize (Zea mays L.) seeds. | Liu Y, Li N, Eom MK, Schumann P, Zhang X, Cao Y, Ge Y, Xiao M, Zhao J, Cheng C, Kim SG | Int J Syst Evol Microbiol | 10.1099/ijsem.0.002341 | 2017 | Bacillus/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, China, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Seeds/*microbiology, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistry, Zea mays/*microbiology | Transcriptome |
Reference
| @id | authors | catalogue | doi/url | title |
|---|---|---|---|---|
| 19165 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 26675) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-26675 | |
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |
| 67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |
| 87170 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID400715.1 | StrainInfo: A central database for resolving microbial strain identifiers | |
| 125438 | Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann | 10.1101/2024.08.12.607695 | Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets | |
| 125439 | Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy | https://github.com/GenomeNet/deepG | deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 |