Strain identifier
BacDive ID: 22904
Type strain:
Species: Castellaniella hirudinis
Strain Designation: E103
Strain history: CIP <- 2012, P. Kämpfer, Giessen Univ., Giessen, Germany: strain E103 <- 2009, L. Kaiser
NCBI tax ID(s): 1144617 (species)
General
@ref: 19219
BacDive-ID: 22904
DSM-Number: 25756
keywords: 16S sequence, Bacteria, anaerobe, mesophilic, Gram-negative, motile, rod-shaped
description: Castellaniella hirudinis E103 is an anaerobe, mesophilic, Gram-negative bacterium that was isolated from skin of a juvenile medical leech .
NCBI tax id
- NCBI tax id: 1144617
- Matching level: species
strain history
@ref | history |
---|---|
19219 | <- P. Kämpfer, Justus-Liebig-Universität, Giessen, Germany; E103 <- L. Kaiser |
119913 | CIP <- 2012, P. Kämpfer, Giessen Univ., Giessen, Germany: strain E103 <- 2009, L. Kaiser |
doi: 10.13145/bacdive22904.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Betaproteobacteria
- order: Burkholderiales
- family: Alcaligenaceae
- genus: Castellaniella
- species: Castellaniella hirudinis
- full scientific name: Castellaniella hirudinis Glaeser et al. 2013
@ref: 19219
domain: Bacteria
phylum: Proteobacteria
class: Betaproteobacteria
order: Burkholderiales
family: Alcaligenaceae
genus: Castellaniella
species: Castellaniella hirudinis
full scientific name: Castellaniella hirudinis Glaeser et al. 2013
strain designation: E103
type strain: yes
Morphology
cell morphology
@ref | gram stain | cell length | cell width | cell shape | motility |
---|---|---|---|---|---|
30662 | negative | 1 µm | 0.5 µm | rod-shaped | yes |
119913 | negative | rod-shaped | yes |
pigmentation
- @ref: 30662
- production: yes
Culture and growth conditions
culture medium
@ref | name | growth | composition | link |
---|---|---|---|---|
32803 | MEDIUM 72- for trypto casein soja agar | yes | Distilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g) | |
19219 | NUTRIENT AGAR (DSMZ Medium 1) | yes | Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water | https://mediadive.dsmz.de/medium/1 |
119913 | CIP Medium 566 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=566 | |
119913 | CIP Medium 72 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
19219 | positive | growth | 28 | mesophilic |
30662 | positive | growth | 15-37 | |
30662 | positive | optimum | 27.5 | mesophilic |
32803 | positive | growth | 25 | mesophilic |
62825 | positive | growth | 30 | mesophilic |
culture pH
@ref | ability | type | pH | PH range |
---|---|---|---|---|
30662 | positive | growth | 5.5-11 | alkaliphile |
30662 | positive | optimum | 8.25 |
Physiology and metabolism
oxygen tolerance
@ref | oxygen tolerance |
---|---|
30662 | anaerobe |
62825 | aerobe |
119913 | obligate aerobe |
spore formation
- @ref: 30662
- spore formation: no
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
30662 | 37054 | 3-hydroxybutyrate | + | carbon source |
30662 | 30089 | acetate | + | carbon source |
30662 | 16449 | alanine | + | carbon source |
30662 | 22599 | arabinose | + | carbon source |
30662 | 35391 | aspartate | + | carbon source |
30662 | 17057 | cellobiose | + | carbon source |
30662 | 16947 | citrate | + | carbon source |
30662 | 28757 | fructose | + | carbon source |
30662 | 28260 | galactose | + | carbon source |
30662 | 24265 | gluconate | + | carbon source |
30662 | 17234 | glucose | + | carbon source |
30662 | 24996 | lactate | + | carbon source |
30662 | 25017 | leucine | + | carbon source |
30662 | 25115 | malate | + | carbon source |
30662 | 17306 | maltose | + | carbon source |
30662 | 37684 | mannose | + | carbon source |
30662 | 506227 | N-acetylglucosamine | + | carbon source |
30662 | 18257 | ornithine | + | carbon source |
30662 | 26271 | proline | + | carbon source |
30662 | 17272 | propionate | + | carbon source |
30662 | 15361 | pyruvate | + | carbon source |
30662 | 17822 | serine | + | carbon source |
30662 | 17992 | sucrose | + | carbon source |
68369 | 25115 | malate | + | assimilation |
68369 | 17128 | adipate | - | assimilation |
68369 | 27689 | decanoate | + | assimilation |
68369 | 24265 | gluconate | + | assimilation |
68369 | 17306 | maltose | - | assimilation |
68369 | 59640 | N-acetylglucosamine | - | assimilation |
68369 | 16899 | D-mannitol | - | assimilation |
68369 | 16024 | D-mannose | - | assimilation |
68369 | 30849 | L-arabinose | - | assimilation |
68369 | 17634 | D-glucose | - | assimilation |
68369 | 5291 | gelatin | - | hydrolysis |
68369 | 4853 | esculin | - | hydrolysis |
68369 | 16199 | urea | - | hydrolysis |
68369 | 29016 | arginine | - | hydrolysis |
68369 | 17634 | D-glucose | - | fermentation |
68369 | 27897 | tryptophan | - | energy source |
68369 | 17632 | nitrate | + | reduction |
119913 | 17632 | nitrate | + | reduction |
119913 | 16301 | nitrite | + | reduction |
metabolite production
@ref | Chebi-ID | metabolite | production |
---|---|---|---|
68369 | 35581 | indole | no |
119913 | 35581 | indole | no |
metabolite tests
- @ref: 68369
- Chebi-ID: 35581
- metabolite: indole
- indole test: -
enzymes
@ref | value | activity | ec |
---|---|---|---|
30662 | catalase | + | 1.11.1.6 |
30662 | cytochrome oxidase | + | 1.9.3.1 |
68369 | cytochrome oxidase | + | 1.9.3.1 |
68369 | gelatinase | - | |
68369 | beta-glucosidase | - | 3.2.1.21 |
68369 | urease | - | 3.5.1.5 |
68369 | arginine dihydrolase | - | 3.5.3.6 |
119913 | oxidase | + | |
119913 | catalase | + | 1.11.1.6 |
119913 | urease | - | 3.5.1.5 |
68382 | alkaline phosphatase | - | 3.1.3.1 |
68382 | esterase (C 4) | + | |
68382 | esterase lipase (C 8) | - | |
68382 | lipase (C 14) | - | |
68382 | leucine arylamidase | + | 3.4.11.1 |
68382 | valine arylamidase | - | |
68382 | cystine arylamidase | - | 3.4.11.3 |
68382 | trypsin | - | 3.4.21.4 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | acid phosphatase | - | 3.1.3.2 |
68382 | naphthol-AS-BI-phosphohydrolase | + | |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | beta-galactosidase | - | 3.2.1.23 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | alpha-glucosidase | - | 3.2.1.20 |
68382 | beta-glucosidase | - | 3.2.1.21 |
68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | alpha-fucosidase | - | 3.2.1.51 |
API zym
@ref | Control | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
119913 | - | - | + | - | - | + | - | - | - | - | - | + | - | - | - | - | - | - | - | - |
API 20NE
@ref | NO3 | TRP | GLU_ Ferm | ADH Arg | URE | ESC | GEL | PNPG | GLU_ Assim | ARA | MNE | MAN | NAG | MAL | GNT | CAP | ADI | MLT | CIT | PAC | OX |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
19219 | + | - | - | - | - | - | - | - | - | - | - | - | - | - | + | + | - | + | + | + | + |
Isolation, sampling and environmental information
isolation
@ref | sample type | host species | geographic location | country | origin.country | continent | isolation date |
---|---|---|---|---|---|---|---|
19219 | skin of a juvenile medical leech (Hirudo verbana) | Hirudo verbana | Hessen, Biebertal | Germany | DEU | Europe | |
62825 | Surface,juvenile leech | Biebertal (Hessen) | Germany | DEU | Europe | ||
119913 | Animal, From the surface of a juvenile leech | Biebertal, Hessen | Germany | DEU | Europe | 2009 |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Host | #Invertebrates (Other) | #Annelida |
#Host Body-Site | #Organ | #Skin, Nail, Hair |
taxonmaps
- @ref: 69479
- File name: preview.99_56092.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_7;96_739;97_862;98_15320;99_56092&stattab=map
- Last taxonomy: Castellaniella
- 16S sequence: JQ319891
- Sequence Identity:
- Total samples: 1905
- soil counts: 445
- aquatic counts: 938
- animal counts: 391
- plant counts: 131
Safety information
risk assessment
@ref | biosafety level | biosafety level comment |
---|---|---|
19219 | 1 | Risk group (German classification) |
119913 | 1 | Risk group (French classification) |
Sequence information
16S sequences
- @ref: 19219
- description: Castellaniella hirudinis strain E103 16S ribosomal RNA gene, partial sequence
- accession: JQ319891
- length: 1368
- database: ena
- NCBI tax ID: 1144617
External links
@ref: 19219
culture collection no.: DSM 25756, CCUG 62394, LMG 26910, CIP 110374
straininfo link
- @ref: 87152
- straininfo: 400990
literature
- topic: Phylogeny
- Pubmed-ID: 22523161
- title: Castellaniella hirudinis sp. nov., isolated from the skin of Hirudo verbana.
- authors: Glaeser SP, Galatis H, Martin K, Kampfer P
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijs.0.041392-0
- year: 2012
- mesh: Alcaligenaceae/*classification/genetics/isolation & purification, Animals, Bacterial Typing Techniques, DNA, Bacterial/genetics, Fatty Acids/analysis, Leeches/*microbiology, Molecular Sequence Data, Nucleic Acid Hybridization, *Phylogeny, Putrescine/analogs & derivatives/analysis, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Ubiquinone/analysis
- topic2: Genetics
Reference
@id | authors | catalogue | doi/url | title | ID_cross_reference | pubmed |
---|---|---|---|---|---|---|
19219 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 25756) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-25756 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
30662 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | 10.1128/mSphere.00237-17 | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 26993 | 28776041 | |
32803 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/8226 | ||||
62825 | Curators of the CCUG | Culture Collection University of Gothenburg (CCUG) (CCUG 62394) | https://www.ccug.se/strain?id=62394 | |||
68369 | Automatically annotated from API 20NE | |||||
68382 | Automatically annotated from API zym | |||||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
87152 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID400990.1 | StrainInfo: A central database for resolving microbial strain identifiers | |||
119913 | Curators of the CIP | Collection of Institut Pasteur (CIP 110374) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20110374 |