Strain identifier

BacDive ID: 2281

Type strain: Yes

Species: Trichococcus flocculiformis

Strain Designation: Echt

Strain history: <- G. Scheff and F. Lingens, strain Echt <- O. Salcher

NCBI tax ID(s): 82803 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 718

BacDive-ID: 2281

DSM-Number: 2094

keywords: genome sequence, 16S sequence, Bacteria

description: Trichococcus flocculiformis Echt is a bacterium that was isolated from sewage sludge, sewage plant.

NCBI tax id

  • NCBI tax id: 82803
  • Matching level: species

strain history

  • @ref: 718
  • history: <- G. Scheff and F. Lingens, strain Echt <- O. Salcher

doi: 10.13145/bacdive2281.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Carnobacteriaceae
  • genus: Trichococcus
  • species: Trichococcus flocculiformis
  • full scientific name: Trichococcus flocculiformis Scheff et al. 1984

@ref: 718

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Carnobacteriaceae

genus: Trichococcus

species: Trichococcus flocculiformis

full scientific name: Trichococcus flocculiformis Scheff et al. 1984 emend. Liu et al. 2002

strain designation: Echt

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram stain
69480no92.117
69480100positive

Culture and growth conditions

culture medium

  • @ref: 718
  • name: TRICHOCOCCUS MEDIUM (DSMZ Medium 183)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/183
  • composition: Name: TRICHOCOCCUS MEDIUM (DSMZ Medium 183) Composition: Tryptone 10.0 g/l Na2SO4 4.0 g/l Glucose 3.0 g/l MgCl2 x 6 H2O 1.1 g/l KCl 0.7 g/l Distilled water

culture temp

  • @ref: 718
  • growth: positive
  • type: growth
  • temperature: 20-30

Physiology and metabolism

spore formation

  • @ref: 69480
  • spore formation: no
  • confidence: 99.839

murein

  • @ref: 718
  • murein short key: A11.31
  • type: A4alpha L-Lys-D-Asp

Isolation, sampling and environmental information

isolation

  • @ref: 718
  • sample type: sewage sludge, sewage plant
  • geographic location: Echterdingen
  • country: Germany
  • origin.country: DEU
  • continent: Europe

isolation source categories

Cat1Cat2Cat3
#Engineered#Industrial#Plant (Factory)
#Engineered#Waste#Sewage sludge

taxonmaps

  • @ref: 69479
  • File name: preview.99_24449.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_13;96_12252;97_14862;98_18388;99_24449&stattab=map
  • Last taxonomy: Trichococcus
  • 16S sequence: Y17301
  • Sequence Identity:
  • Total samples: 38677
  • soil counts: 4890
  • aquatic counts: 19650
  • animal counts: 13208
  • plant counts: 929

Safety information

risk assessment

  • @ref: 718
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Trichococcus flocculiformis 16S rRNA gene, strain DSM 2094AJ3066111438ena82803
718Trichococcus flocculiformis 16S rRNA geneY173011550ena82803

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Trichococcus flocculiformis DSM 2094GCA_900113645scaffoldncbi82803
66792Trichococcus flocculiformis strain DSM 209482803.9wgspatric82803
66792Trichococcus flocculiformis DSM 20942619618836draftimg82803

GC content

  • @ref: 718
  • GC-content: 46.8
  • method: Buoyant density centrifugation (BD)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes52no
motileno94.042no
flagellatedno93.041no
gram-positiveyes91.77no
anaerobicno78.591no
aerobicno92.269no
halophileyes66.519no
spore-formingno87.879no
glucose-fermentyes77.94no
thermophileno99.607no
glucose-utilyes89.446no

External links

@ref: 718

culture collection no.: DSM 2094, ATCC 51221

straininfo link

  • @ref: 71839
  • straininfo: 42865

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny12148615Emended description of the genus Trichococcus, description of Trichococcus collinsii sp. nov., and reclassification of Lactosphaera pasteurii as Trichococcus pasteurii comb. nov. and of Ruminococcus palustris as Trichococcus palustris comb. nov. in the low-G+C gram-positive bacteria.Liu JR, Tanner RS, Schumann P, Weiss N, McKenzie CA, Janssen PH, Seviour EM, Lawson PA, Allen TD, Seviour RJInt J Syst Evol Microbiol10.1099/00207713-52-4-11132002Bacteria/*classification/genetics/metabolism/ultrastructure, Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, DNA, Bacterial/analysis/genetics, DNA, Ribosomal/analysis/genetics, Fatty Acids/analysis, Genes, rRNA, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, RNA, Ribosomal, 16S/genetics, RNA, Ribosomal, 23S/geneticsGenetics
Phylogeny31914924Genome-guided analysis allows the identification of novel physiological traits in Trichococcus species.Strepis N, Naranjo HD, Meier-Kolthoff J, Goker M, Shapiro N, Kyrpides N, Klenk HP, Schaap PJ, Stams AJM, Sousa DZBMC Genomics10.1186/s12864-019-6410-x2020Bacterial Typing Techniques, Carnobacteriaceae/*genetics/metabolism/*physiology, Phenotype, Phylogeny, Propylene Glycols/metabolism, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNAMetabolism

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
718Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 2094)https://www.dsmz.de/collection/catalogue/details/culture/DSM-2094
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
71839Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID42865.1StrainInfo: A central database for resolving microbial strain identifiers