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Strain identifier

BacDive ID: 2277

Type strain: Yes

Species: Isobaculum melis

Strain Designation: M577-94

Strain history: <- M.D. Collins <- G. Foster; M577-94

NCBI tax ID(s): 142588 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 5153

BacDive-ID: 2277

DSM-Number: 13760

keywords: mesophilic, microaerophile, Bacteria, 16S sequence, genome sequence

description: Isobaculum melis M577-94 is a microaerophile, mesophilic bacterium that was isolated from small intestine of dead badger.

NCBI tax id

  • NCBI tax id: 142588
  • Matching level: species

strain history: <- M.D. Collins <- G. Foster; M577-94

doi: 10.13145/bacdive2277.20220920.7

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Carnobacteriaceae
  • genus: Isobaculum
  • species: Isobaculum melis
  • full scientific name: Isobaculum melis Collins et al. 2002

@ref: 5153

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Carnobacteriaceae

genus: Isobaculum

species: Isobaculum melis

full scientific name: Isobaculum melis Collins et al. 2002

strain designation: M577-94

type strain: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5153COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://bacmedia.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
5153PYG MEDIUM (MODIFIED) (DSMZ Medium 104)yeshttps://bacmedia.dsmz.de/medium/104Name: PYG MEDIUM (modified) (DSMZ Medium 104) Composition: Yeast extract 10.0 g/l Peptone 5.0 g/l Trypticase peptone 5.0 g/l Beef extract 5.0 g/l Glucose 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l K2HPO4 0.04 g/l KH2PO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Hemin 0.005 g/l Ethanol 0.0038 g/l Resazurin 0.001 g/l Tween 80 Vitamin K1 NaOH Distilled water
33781MEDIUM 6 - Columbia agar with 10 % horse bloodyesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml)

culture temp

@refgrowthtypetemperaturerange
5153positivegrowth37mesophilic
33781positivegrowth37mesophilic
53790positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 53790
  • oxygen tolerance: microaerophile

murein

  • @ref: 5153
  • murein short key: A11.24
  • type: A3alpha L-Lys-L-Thr-Gly

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinent
5153small intestine of dead badgerUnited KingdomGBREurope
53790Badger

isolation source categories

Cat1Cat2Cat3
#Host#Other#Decomposing animal
#Host#Mammals
#Host Body-Site#Gastrointestinal tract#Small intestine

Safety information

risk assessment

  • @ref: 5153
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 5153
  • description: Isobaculum melis 16S rRNA gene, isolate CCUG 37660T
  • accession: AJ302648
  • length: 1515
  • database: ena
  • NCBI tax ID: 142588

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Isobaculum melis DSM 13760GCA_900111355scaffoldncbi142588
66792Isobaculum melis strain DSM 13760142588.3wgspatric142588
66792Isobaculum melis DSM 137602634166323draftimg142588

GC content

  • @ref: 5153
  • GC-content: 39
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 5153

culture collection no.: DSM 13760, CCUG 37660, CIP 107375

straininfo link

@refpassport
20218http://www.straininfo.net/strains/302711
20218http://www.straininfo.net/strains/278203

literature

  • topic: Phylogeny
  • Pubmed-ID: 11837304
  • title: Isobaculum melis gen. nov., sp. nov., a Carnobacterium-like organism isolated from the intestine of a badger.
  • authors: Collins MD, Hutson RA, Foster G, Falsen E, Weiss N
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/00207713-52-1-207
  • year: 2002
  • mesh: Anaerobiosis, Animals, Bacterial Typing Techniques, Carnivora/*microbiology, DNA, Ribosomal/analysis, Gram-Positive Rods/*classification/genetics/*isolation & purification, Intestines/*microbiology, Molecular Sequence Data, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
5153Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 13760)https://www.dsmz.de/collection/catalogue/details/culture/DSM-13760
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
33781Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/4803
53790Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 37660)https://www.ccug.se/strain?id=37660
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880