Strain identifier

BacDive ID: 2263

Type strain: No

Species: Carnobacterium maltaromaticum

Strain Designation: B270

Strain history: CIP <- 1988, NCFB, Carnobacterium piscicola <- S.F. Hiu: strain B270, Lactobacillus piscicola <- R.A. Holt

NCBI tax ID(s): 1087479 (strain), 2751 (species)

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General

@ref: 9040

BacDive-ID: 2263

DSM-Number: 20730

keywords: genome sequence, 16S sequence, Bacteria, spore-forming, mesophilic, animal pathogen

description: Carnobacterium maltaromaticum B270 is a spore-forming, mesophilic animal pathogen that was isolated from diseased rainbow trout.

NCBI tax id

NCBI tax idMatching level
1087479strain
2751species

strain history

@refhistory
9040<- ATCC <- R.A. Holt, B270 (Lactobacillus piscicola)
67770J. L. Fryer B 270.
120767CIP <- 1988, NCFB, Carnobacterium piscicola <- S.F. Hiu: strain B270, Lactobacillus piscicola <- R.A. Holt

doi: 10.13145/bacdive2263.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Carnobacteriaceae
  • genus: Carnobacterium
  • species: Carnobacterium maltaromaticum
  • full scientific name: Carnobacterium maltaromaticum corrig. (Miller et al. 1974) Mora et al. 2003

  • synonyms

    @refsynonym
    20215Lactobacillus maltaromicus
    20215Lactobacillus carnis
    20215Carnobacterium piscicola
    20215Lactobacillus piscicola

@ref: 9040

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Carnobacteriaceae

genus: Carnobacterium

species: Carnobacterium maltaromaticum

full scientific name: Carnobacterium maltaromaticum (Miller et al. 1974) Mora et al. 2003

strain designation: B270

type strain: no

Morphology

cell morphology

@refmotilityconfidencegram stain
69480no91.99
69480100positive

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
9040TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
34121MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
120767CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72
120767CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3

culture temp

@refgrowthtypetemperaturerange
9040positivegrowth30mesophilic
34121positivegrowth30mesophilic
52230positivegrowth37mesophilic
52230positivegrowth22-26
67770positivegrowth30mesophilic

Physiology and metabolism

spore formation

@refspore formationconfidence
69481yes100
69480no99.922

murein

  • @ref: 9040
  • murein short key: A31
  • type: A1gamma m-Dpm-direct

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6837917632nitrate-reduction
683794853esculin+hydrolysis
6837916199urea-hydrolysis
683795291gelatin-hydrolysis
6837917634D-glucose+fermentation
6837916988D-ribose+fermentation
6837965327D-xylose-fermentation
6837916899D-mannitol+fermentation
6837917306maltose+fermentation
6837917716lactose+fermentation
6837917992sucrose+fermentation
6837928087glycogen-fermentation
6838129016arginine+hydrolysis
6838116988D-ribose+builds acid from
6838116899D-mannitol-builds acid from
6838130911sorbitol-builds acid from
6838117716lactose-builds acid from
6838127082trehalose+builds acid from
6838116634raffinose-builds acid from
6838117992sucrose+builds acid from
6838130849L-arabinose-builds acid from
6838118333D-arabitol-builds acid from
6838140585alpha-cyclodextrin+builds acid from
68381606565hippurate-hydrolysis
6838128087glycogen-builds acid from
6838127941pullulan-builds acid from
6838117306maltose+builds acid from
6838128053melibiose-builds acid from
683816731melezitose-builds acid from
68381320055methyl beta-D-glucopyranoside+builds acid from
6838116443D-tagatose-builds acid from
6838116199urea-hydrolysis

metabolite production

  • @ref: 68381
  • Chebi-ID: 15688
  • metabolite: acetoin
  • production: yes

metabolite tests

  • @ref: 68381
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: +

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase-3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase-3.1.3.1
68381urease-3.5.1.5
68381beta-mannosidase+3.2.1.25
68381glycyl tryptophan arylamidase+
68381N-acetyl-beta-glucosaminidase+3.2.1.52
68381pyrrolidonyl arylamidase+3.4.19.3
68381beta-galactosidase-3.2.1.23
68381Alanyl-Phenylalanyl-Proline arylamidase-
68381alkaline phosphatase-3.1.3.1
68381alpha-galactosidase+3.2.1.22
68381beta-glucuronidase-3.2.1.31
68381beta-glucosidase+3.2.1.21
68381arginine dihydrolase+3.5.3.6
68379catalase+1.11.1.6
68379gelatinase-
68379urease-3.5.1.5
68379beta-glucosidase+3.2.1.21
68379N-acetyl-beta-glucosaminidase+3.2.1.52
68379alpha-glucosidase+3.2.1.20
68379beta-galactosidase+3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase-3.1.3.1
68379pyrrolidonyl arylamidase+3.4.19.3
68379pyrazinamidase+3.5.1.B15

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELControlGLURIBXYLMANMALLACSACGLYGCAT
52230-++--++++---++-++++-+

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
52230--++------++---++---

API rID32STR

@refADH Argbeta GLUbeta GARbeta GURalpha GALPALRIBMANSORLACTRERAFSACLARADARLCDEXVPAPPAbeta GALPyrAbeta NAGGTAHIPGLYGPULMALMELMLZMbeta DGTAGbeta MANURE
52230++--+-+---+-+--++--+++---+--+-+-

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation date
9040diseased rainbow trout
52230Fish,kidney of adult cutthroat trout,Salmo clarkiOregonUSAUSANorth America
67770Diseased adult cutthroat trout
120767Human, Adult cutthroat trout, kidneyCoos county, OregonUnited States of AmericaUSANorth America1970

isolation source categories

  • Cat1: #Host
  • Cat2: #Fishes
  • Cat3: #Salmonidae

taxonmaps

  • @ref: 69479
  • File name: preview.99_2222.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_13;96_41;97_1423;98_1732;99_2222&stattab=map
  • Last taxonomy: Carnobacterium maltaromaticum
  • 16S sequence: LC269269
  • Sequence Identity:
  • Total samples: 12837
  • soil counts: 1440
  • aquatic counts: 2720
  • animal counts: 8257
  • plant counts: 420

Safety information

risk assessment

@refpathogenicity animalbiosafety levelbiosafety level comment
9040yes2Risk group (German classification)
1207671Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Carnobacterium piscicola 16S ribosomal RNA gene, partial sequence; small 16S-23S ribosomal RNA intergenic spacer, complete sequence; and 23S ribosomal RNA gene, partial sequenceAF374295619ena2751
20218Carnobacterium piscicola 16S ribosomal RNA gene, partial sequence; medium 16S-23S intergenic spacer and tRNA-Ala gene, complete sequence; and 23S ribosomal RNA gene, partial sequenceAF374296701ena2751
20218Carnobacterium piscicola 16S ribosomal RNA gene, partial sequence; large 16S-23S intergenic spacer, tRNA-Ile and tRNA-Ala genes, complete sequence; and 23S ribosomal RNA gene, partial sequenceAF374297830ena2751
20218Carnobacterium maltaromaticum strain ATCC 35586 16S ribosomal RNA gene, partial sequenceJF749289809ena1087479
20218Carnobacterium piscicola partial 16S ribosomal RNAX542681492ena2751
67770Carnobacterium maltaromaticum gene for 16S ribosomal RNA, partial sequence, strain: JCM 5348LC2692691495ena2751

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Carnobacterium maltaromaticum ATCC 355861087479.3wgspatric1087479
66792Carnobacterium maltaromaticum strain DSM 207302751.34wgspatric2751
66792Carnobacterium maltaromaticum ATCC 355862547132401draftimg1087479
66792Carnobacterium maltaromaticum DSM 207302660238721draftimg2751
67770Carnobacterium maltaromaticum ATCC 35586GCA_000238575contigncbi1087479
67770Carnobacterium maltaromaticum DSM 20730GCA_001438765scaffoldncbi2751

GC content

@refGC-contentmethod
904035.6
904034.5
6777034.5thermal denaturation, midpoint method (Tm)
6777035.6thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes100no
motileno94.11no
flagellatedno95.45no
gram-positiveyes94.157no
anaerobicno95.968no
aerobicno91.262no
halophileyes71.342no
spore-formingno79.691no
thermophileno99.942yes
glucose-utilyes89.777no
glucose-fermentyes84.354yes

External links

@ref: 9040

culture collection no.: DSM 20730, ATCC 35586, NCDO 2762, CCUG 34645, JCM 5348, CCM 3842, CECT 4020, CIP 103158, HAMBI 2234, IFO 15684, KCTC 3676, LMG 9839, NBRC 15684, NCCB 91131, NCFB 2762, NCIMB 2264, NRRL B-14829

straininfo link

  • @ref: 71822
  • straininfo: 1688

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny1781676Biochemical and serological characterization of Carnobacterium spp. isolated from farmed and natural populations of striped bass and catfish.Baya AM, Toranzo AE, Lupiani B, Li T, Roberson BS, Hetrick FMAppl Environ Microbiol10.1128/aem.57.11.3114-3120.19911991Animals, Bass/*microbiology, Fish Diseases/microbiology, Gram-Positive Asporogenous Rods/*classification/isolation & purification, Ictaluridae/*microbiology, Lactobacillaceae/classification/isolation & purification/pathogenicity, Phenotype, Serotyping, Trout/microbiology, VirulenceEnzymology
Pathogenicity12526879Immunomodulatory effects of nisin in turbot (Scophthalmus maximus L.).Villamil L, Figueras A, Novoa BFish Shellfish Immunol10.1006/fsim.2002.04252003Analysis of Variance, Animals, Anti-Bacterial Agents/*immunology/pharmacology, Cells, Cultured, Dose-Response Relationship, Immunologic, Flatfishes/*immunology, Kidney/cytology/immunology, Leukocytes/drug effects/immunology, Luminescent Measurements, Macrophages/*drug effects/immunology, Nisin/*immunology/pharmacology, Phagocytosis/drug effects, Time Factors
Biotechnology12793859Enhancement of nisin production by Lactococcus lactis in periodically re-alkalized cultures.Guerra NP, Castro LPBiotechnol Appl Biochem10.1042/BA200300592003Animals, Bacteriocins/pharmacology, Biotechnology/methods, Bivalvia/metabolism, Culture Media/chemistry, Fermentation, Glucose/metabolism, Humans, Hydrogen-Ion Concentration, Industrial Waste, Lactococcus lactis/growth & development/*metabolism, Male, Milk Proteins, Nisin/*biosynthesis, Time Factors, Whey ProteinsMetabolism
Biotechnology12929829Inhibition of Listeria monocytogenes in cold-smoked salmon by Carnobacterium piscicola CS526 isolated from frozen surimi.Yamazaki K, Suzuki M, Kawai Y, Inoue N, Montville TJJ Food Prot10.4315/0362-028x-66.8.14202003Animals, Antibiosis, Bacteriocins/biosynthesis/*pharmacology, Colony Count, Microbial, Fish Products/*microbiology, Food Handling/*methods, Food Microbiology, Food Packaging, Food Preservation/methods, Lactobacillaceae/metabolism/*physiology, Listeria monocytogenes/*growth & development, Salmon/*microbiology, Temperature, Time FactorsMetabolism
Metabolism19191975Selected nondigestible carbohydrates and prebiotics support the growth of probiotic fish bacteria mono-cultures in vitro.Rurangwa E, Laranja JL, Van Houdt R, Delaedt Y, Geraylou Z, Van de Wiele T, Van Loo J, Van Craeyveld V, Courtin CM, Delcour JA, Ollevier FJ Appl Microbiol10.1111/j.1365-2672.2008.04034.x2009Acetic Acid/metabolism, Animals, Bacillus subtilis/growth & development, Bacteria/*growth & development/metabolism, Bacteriological Techniques, *Carbohydrate Metabolism, Carnobacterium/growth & development, Colony Count, Microbial, Culture Media, Fishes/microbiology, Glucose/metabolism, Inulin/metabolism, Lactobacillus/growth & development, Oligosaccharides/metabolism, *Prebiotics, *Probiotics, beta-Glucans/metabolismCultivation
Metabolism21704418The genome sequence of the lactic acid bacterium, Carnobacterium maltaromaticum ATCC 35586 encodes potential virulence factors.Leisner JJ, Hansen MA, Larsen MH, Hansen L, Ingmer H, Sorensen SJInt J Food Microbiol10.1016/j.ijfoodmicro.2011.05.0122011Animals, Carnobacterium/*genetics/metabolism/*pathogenicity, Drug Resistance, Bacterial, Fishes/microbiology, Gene Expression Regulation, Bacterial, *Genome, Bacterial, Lactic Acid/metabolism, Listeria monocytogenes/genetics, Meat Products/microbiology, Peptide Termination Factors/metabolism, Virulence Factors/*genetics/metabolismGenetics
Pathogenicity22588175Antibacterial activity of class IIa bacteriocin Cbn BM1 depends on the physiological state of the target bacteria.Jacquet T, Cailliez-Grimal C, Francius G, Borges F, Imran M, Duval JF, Revol-Junelles AMRes Microbiol10.1016/j.resmic.2012.04.0012012Anti-Bacterial Agents/*pharmacology, Bacteriocins/*pharmacology, Carnobacterium/*drug effects, Cell Membrane/drug effects/physiology/ultrastructure, Fluorescence Polarization, Membrane Fluidity/drug effects, Microscopy, Atomic Force, Proton-Motive Force/drug effects
Genetics32627213Genome-wide comparison of Carnobacterium maltaromaticum derived from diseased fish harbouring important virulence-related genes.Roh H, Kim BS, Lee MK, Park CI, Kim DHJ Fish Dis10.1111/jfd.132082020Animals, Aquaculture, Carnobacterium/*genetics/*pathogenicity, Fish Diseases/*microbiology, Genome, Bacterial, Gram-Positive Bacterial Infections/microbiology/*veterinary, Oncorhynchus mykiss, Republic of Korea, Virulence/*genetics
Metabolism34683489Identification of Potential Citrate Metabolism Pathways in Carnobacterium maltaromaticum.Li H, Ramia NE, Borges F, Revol-Junelles AM, Vogensen FK, Leisner JJMicroorganisms10.3390/microorganisms91021692021

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
9040Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20730)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20730
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
34121Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/14925
52230Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 34645)https://www.ccug.se/strain?id=34645
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68379Automatically annotated from API Coryne
68381Automatically annotated from API rID32STR
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
71822Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID1688.1StrainInfo: A central database for resolving microbial strain identifiers
120767Curators of the CIPCollection of Institut Pasteur (CIP 103158)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103158