Strain identifier

BacDive ID: 226

Type strain: Yes

Species: Aerococcus christensenii

Strain Designation: UW06, UW 06

Strain history: CIP <- 1999, CCUG <- 1991, L.K. Rabe & S.L. Hillier, Seattle, USA: strain UW 06

NCBI tax ID(s): 525247 (strain), 87541 (species)

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General

@ref: 6123

BacDive-ID: 226

DSM-Number: 15819

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, Gram-positive, coccus-shaped, human pathogen

description: Aerococcus christensenii UW06 is a microaerophile, mesophilic, Gram-positive human pathogen that was isolated from human vagina.

NCBI tax id

NCBI tax idMatching level
87541species
525247strain

strain history

@refhistory
6123<- E. Falsen, CCUG <- L. K. Rabe
67770CIP 106115 <-- CCUG 28831 <-- L. K. Rabe and S. L. Hillier UW 06.
118258CIP <- 1999, CCUG <- 1991, L.K. Rabe & S.L. Hillier, Seattle, USA: strain UW 06

doi: 10.13145/bacdive226.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Aerococcaceae
  • genus: Aerococcus
  • species: Aerococcus christensenii
  • full scientific name: Aerococcus christensenii Collins et al. 1999

@ref: 6123

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Aerococcaceae

genus: Aerococcus

species: Aerococcus christensenii

full scientific name: Aerococcus christensenii Collins et al. 1999 emend. Tohno et al. 2014

strain designation: UW06, UW 06

type strain: yes

Morphology

cell morphology

@refgram staincell shapemotilityconfidence
22979positivecoccus-shapedno
69480no95.969
69480positive100
118258positivecoccus-shapedno

colony morphology

@reftype of hemolysishemolysis abilitymedium used
22979alpha1blood agar
118258

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
6123COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
6123PYG MEDIUM (MODIFIED) (DSMZ Medium 104)yeshttps://mediadive.dsmz.de/medium/104Name: PYG MEDIUM (modified) (DSMZ Medium 104) Composition: Yeast extract 10.0 g/l Peptone 5.0 g/l Trypticase peptone 5.0 g/l Beef extract 5.0 g/l Glucose 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l K2HPO4 0.04 g/l KH2PO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Hemin 0.005 g/l Ethanol 0.0038 g/l Resazurin 0.001 g/l Tween 80 Vitamin K1 NaOH Distilled water
36412MEDIUM 6 - Columbia agar with 10 % horse bloodyesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml)
118258CIP Medium 6yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=6

culture temp

@refgrowthtypetemperaturerange
6123positivegrowth37mesophilic
36412positivegrowth30mesophilic
49410positivegrowth37mesophilic
67770positivegrowth30mesophilic
118258positivegrowth30-37mesophilic
118258nogrowth10psychrophilic
118258nogrowth25mesophilic
118258nogrowth41thermophilic
118258nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
6123microaerophile
22979facultative anaerobe
49410anaerobe
49410microaerophile
118258facultative anaerobe

spore formation

@refspore formationconfidence
22979no
69481no99
69480no99.992

halophily

  • @ref: 22979
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 6.5 %

murein

  • @ref: 6123
  • murein short key: A11
  • type: A1alpha L-Lys-direct

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2297923456cyclodextrin-builds acid from
2297918333D-arabitol-builds acid from
2297928087glycogen-builds acid from
2297930849L-arabinose-builds acid from
2297917716lactose-builds acid from
2297917306maltose-builds acid from
2297929864mannitol-builds acid from
229796731melezitose-builds acid from
2297928053melibiose-builds acid from
22979320055methyl beta-D-glucopyranoside-builds acid from
2297927941pullulan-builds acid from
2297916634raffinose-builds acid from
2297933942ribose-builds acid from
2297930911sorbitol-builds acid from
2297917992sucrose-builds acid from
2297933954tagatose-builds acid from
2297927082trehalose-builds acid from
22979606565hippurate+hydrolysis
1182584853esculin-hydrolysis
11825817632nitrate-reduction
11825816301nitrite-reduction
6837516199urea-hydrolysis
6837529016arginine-hydrolysis
6837518257ornithine-degradation
683754853esculin-hydrolysis
6837517634D-glucose-fermentation
6837515824D-fructose-fermentation
6837516024D-mannose-fermentation
6837517306maltose-fermentation
6837517716lactose-fermentation
6837527082trehalose-fermentation
6837516899D-mannitol-fermentation
6837516634raffinose-fermentation
6837516988D-ribose-fermentation
6837517057cellobiose-fermentation
6837517632nitrate-reduction
6837517992sucrose-fermentation
6837559640N-acetylglucosamine-fermentation
6837532528turanose-fermentation
6837522599arabinose-fermentation
6838129016arginine-hydrolysis
6838116988D-ribose-builds acid from
6838116899D-mannitol-builds acid from
6838130911sorbitol-builds acid from
6838117716lactose-builds acid from
6838127082trehalose-builds acid from
6838116634raffinose-builds acid from
6838117992sucrose-builds acid from
6838130849L-arabinose-builds acid from
6838118333D-arabitol-builds acid from
6838140585alpha-cyclodextrin-builds acid from
68381606565hippurate-hydrolysis
6838128087glycogen-builds acid from
6838127941pullulan-builds acid from
6838117306maltose-builds acid from
6838128053melibiose-builds acid from
68381320055methyl beta-D-glucopyranoside-builds acid from
6838116443D-tagatose-builds acid from
6838116199urea-hydrolysis

antibiotic resistance

  • @ref: 68375
  • ChEBI: 28368
  • metabolite: novobiocin
  • is antibiotic: yes
  • is sensitive: yes
  • sensitivity conc.: 1.8 µg

metabolite production

@refChebi-IDmetaboliteproduction
2297915688acetoinno
6837515688acetoinno
11825835581indoleno
6838115688acetoinno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-test
6838115688acetoin-
6837515688acetoin-
11825815688acetoin-

enzymes

@refvalueactivityec
22979alkaline phosphatase-3.1.3.1
22979alpha-galactosidase-3.2.1.22
22979arginine dihydrolase-3.5.3.6
22979beta-galactosidase-3.2.1.23
22979beta-glucosidase-3.2.1.21
22979beta-glucuronidase-3.2.1.31
22979beta-mannosidase-3.2.1.25
22979catalase-1.11.1.6
22979cytochrome oxidase-1.9.3.1
22979glycyl tryptophan arylamidase-
22979N-acetyl-beta-glucosaminidase-3.2.1.52
22979pyroglutamic acid arylamidase-
22979tripeptide aminopeptidase-3.4.11.4
22979urease-3.5.1.5
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase-3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)-
68382alkaline phosphatase-3.1.3.1
68381urease-3.5.1.5
68381beta-mannosidase-3.2.1.25
68381glycyl tryptophan arylamidase-
68381N-acetyl-beta-glucosaminidase-3.2.1.52
68381pyrrolidonyl arylamidase-3.4.19.3
68381beta-galactosidase-3.2.1.23
68381Alanyl-Phenylalanyl-Proline arylamidase+
68381alkaline phosphatase-3.1.3.1
68381alpha-galactosidase-3.2.1.22
68381beta-glucuronidase-3.2.1.31
68381beta-glucosidase-3.2.1.21
68381arginine dihydrolase-3.5.3.6
68375beta-glucuronidase-3.2.1.31
68375pyrrolidonyl arylamidase-3.4.19.3
68375alkaline phosphatase-3.1.3.1
68375L-arginine arylamidase+
68375beta-galactosidase-3.2.1.23
68375beta-glucosidase-3.2.1.21
68375ornithine decarboxylase-4.1.1.17
68375arginine dihydrolase-3.5.3.6
68375urease-3.5.1.5
118258oxidase-
118258beta-galactosidase-3.2.1.23
118258alcohol dehydrogenase-1.1.1.1
118258catalase+1.11.1.6
118258gamma-glutamyltransferase-2.3.2.2
118258lysine decarboxylase-4.1.1.18
118258ornithine decarboxylase-4.1.1.17
118258urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
118258---+------++---+----

API ID32STA

@refUREADH ArgODCESCGLUFRUMNEMALLACTREMANRAFRIBCELNITVPbeta GALArgAPALPyrANOVOSACNAGTURARAbeta GUR
49410-----------------+--------

API rID32STR

@refADH Argbeta GLUbeta GARbeta GURalpha GALPALRIBMANSORLACTRERAFSACLARADARLCDEXVPAPPAbeta GALPyrAbeta NAGGTAHIPGLYGPULMALMELMbeta DGTAGbeta MANURE
6123-----------------+-------------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
118258---------------------------------------------------------------------------------------------------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation date
6123human vaginaSeattleUSAUSANorth America
49410Human vaginaSeattleUSAUSANorth America
67770Human vagina
118258Human, VaginaSeattle, WashingtonUnited States of AmericaUSANorth America1991

isolation source categories

Cat1Cat2Cat3
#Host Body-Site#Urogenital tract#Vagina
#Host#Human#Female

taxonmaps

  • @ref: 69479
  • File name: preview.99_4106.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_13;96_2083;97_2510;98_3104;99_4106&stattab=map
  • Last taxonomy: Aerococcus christensenii subclade
  • 16S sequence: LC589208
  • Sequence Identity:
  • Total samples: 32289
  • soil counts: 773
  • aquatic counts: 1099
  • animal counts: 30254
  • plant counts: 163

Safety information

risk assessment

@refpathogenicity humanbiosafety levelbiosafety level comment
6123yes, in single cases1Risk group (German classification)
1182581Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Aerococcus christensenii strain CCUG 28831 16S-23S ribosomal RNA intergenic spacer, partial sequenceJN977130212ena87541
6123Aerococcus christensenii 16S ribosomal RNAY170051451ena87541
67770Aerococcus christensenii JCM 18985 gene for 16S rRNA, partial sequenceLC5892081485ena87541

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Aerococcus christensenii CCUG28831GCA_001543105completencbi87541
66792Aerococcus christensenii DSM 15819 = CCUG 28831525247.5wgspatric525247
66792Aerococcus christensenii strain CCUG2883187541.3completepatric87541
66792Aerococcus christensenii DSM 158192728369595draftimg525247
66792Aerococcus christensenii CCUG288312687453475completeimg87541
67770Aerococcus christensenii DSM 15819 = CCUG 28831GCA_001466745contigncbi525247

GC content

@refGC-contentmethod
612338.5
612339.8high performance liquid chromatography (HPLC)
6777038.5thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno99no
flagellatedno98.511yes
gram-positiveyes92.867no
anaerobicno77.145no
aerobicno97.444yes
halophileyes94.444yes
spore-formingno94.891yes
thermophileno95.272yes
glucose-utilyes86.978no
motileno96.458no
glucose-fermentyes79.199yes

External links

@ref: 6123

culture collection no.: DSM 15819, CCM 4911, CCUG 28831, CIP 106115, JCM 18985, ATCC 48849, LMG 19525

straininfo link

  • @ref: 69909
  • straininfo: 13113

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny10425770Aerococcus christensenii sp. nov., from the human vagina.Collins MD, Jovita MR, Hutson RA, Ohlen M, Falsen EInt J Syst Bacteriol10.1099/00207713-49-3-11251999Bacterial Proteins/chemistry, Bacterial Typing Techniques, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Electrophoresis, Polyacrylamide Gel, Female, Genes, rRNA, Gram-Positive Cocci/chemistry/*classification/genetics/*isolation & purification, Humans, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Vagina/*microbiologyEnzymology
Genetics27103727Complete Genome Sequences of Aerococcus christensenii CCUG 28831T, Aerococcus sanguinicola CCUG 43001T, Aerococcus urinae CCUG 36881T, Aerococcus urinaeequi CCUG 28094T, Aerococcus urinaehominis CCUG 42038 BT, and Aerococcus viridans CCUG 4311T.Carkaci D, Dargis R, Nielsen XC, Skovgaard O, Fuursted K, Christensen JJGenome Announc10.1128/genomeA.00302-162016

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
6123Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 15819)https://www.dsmz.de/collection/catalogue/details/culture/DSM-15819
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
22979Matthew D. Collins,Mar Rodriguez Jovita,Roger A. Hutson,Maria Ohlén,Enevold Falsen10.1099/00207713-49-3-1125Aerococcus christensenii sp. nov., from the human vaginaIJSEM 49: 1125-1128 199910425770
36412Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/18210
49410Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 28831)https://www.ccug.se/strain?id=28831
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68375Automatically annotated from API ID32STA
68381Automatically annotated from API rID32STR
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
69909Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID13113.1StrainInfo: A central database for resolving microbial strain identifiers
118258Curators of the CIPCollection of Institut Pasteur (CIP 106115)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20106115