Strain identifier
BacDive ID: 2258
Type strain:
Species: Carnobacterium maltaromaticum
Strain Designation: MX 5, MX5
Strain history: CIP <- 1988, DSM <- M.E. Morgan: strain MX5
NCBI tax ID(s): 1449341 (strain), 2751 (species)
General
@ref: 8747
BacDive-ID: 2258
DSM-Number: 20342
keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-positive, animal pathogen
description: Carnobacterium maltaromaticum MX 5 is an aerobe, mesophilic, Gram-positive animal pathogen that was isolated from milk with malty flavour.
NCBI tax id
NCBI tax id | Matching level |
---|---|
1449341 | strain |
2751 | species |
strain history
@ref | history |
---|---|
8747 | <- M.E. Morgan, MX 5 |
67770 | ATCC 27865 <-- M. E. Morgan MX5. |
120768 | CIP <- 1988, DSM <- M.E. Morgan: strain MX5 |
doi: 10.13145/bacdive2258.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Bacilli
- order: Lactobacillales
- family: Carnobacteriaceae
- genus: Carnobacterium
- species: Carnobacterium maltaromaticum
- full scientific name: Carnobacterium maltaromaticum corrig. (Miller et al. 1974) Mora et al. 2003
synonyms
@ref synonym 20215 Lactobacillus maltaromicus 20215 Lactobacillus carnis 20215 Carnobacterium piscicola 20215 Lactobacillus piscicola
@ref: 8747
domain: Bacteria
phylum: Firmicutes
class: Bacilli
order: Lactobacillales
family: Carnobacteriaceae
genus: Carnobacterium
species: Carnobacterium maltaromaticum
full scientific name: Carnobacterium maltaromaticum (Miller et al. 1974) Mora et al. 2003
strain designation: MX 5, MX5
type strain: yes
Morphology
cell morphology
@ref | gram stain | confidence | cell shape | motility |
---|---|---|---|---|
69480 | positive | 100 | ||
120768 | positive | rod-shaped | no |
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
8747 | COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) | yes | https://mediadive.dsmz.de/medium/693 | Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base |
8747 | TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) | yes | https://mediadive.dsmz.de/medium/92 | Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water |
39686 | MEDIUM 72- for trypto casein soja agar | yes | Distilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g) | |
120768 | CIP Medium 72 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72 | |
120768 | CIP Medium 29 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=29 | |
120768 | CIP Medium 3 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
8747 | positive | growth | 30 | mesophilic |
39686 | positive | growth | 30 | mesophilic |
49935 | positive | growth | 30 | mesophilic |
67770 | positive | growth | 30 | mesophilic |
120768 | positive | growth | 15-37 | |
120768 | no | growth | 45 | thermophilic |
Physiology and metabolism
oxygen tolerance
@ref | oxygen tolerance |
---|---|
49935 | aerobe |
120768 | facultative anaerobe |
spore formation
- @ref: 69480
- spore formation: no
- confidence: 99.887
murein
- @ref: 8747
- murein short key: A31
- type: A1gamma m-Dpm-direct
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
120768 | 17632 | nitrate | - | reduction |
120768 | 16301 | nitrite | - | reduction |
120768 | 17632 | nitrate | + | respiration |
68379 | 17632 | nitrate | - | reduction |
68379 | 4853 | esculin | + | hydrolysis |
68379 | 16199 | urea | - | hydrolysis |
68379 | 5291 | gelatin | - | hydrolysis |
68379 | 17634 | D-glucose | + | fermentation |
68379 | 16988 | D-ribose | + | fermentation |
68379 | 65327 | D-xylose | - | fermentation |
68379 | 16899 | D-mannitol | - | fermentation |
68379 | 17306 | maltose | + | fermentation |
68379 | 17716 | lactose | + | fermentation |
68379 | 17992 | sucrose | + | fermentation |
68379 | 28087 | glycogen | - | fermentation |
68381 | 29016 | arginine | - | hydrolysis |
68381 | 16988 | D-ribose | + | builds acid from |
68381 | 16899 | D-mannitol | - | builds acid from |
68381 | 30911 | sorbitol | - | builds acid from |
68381 | 17716 | lactose | + | builds acid from |
68381 | 27082 | trehalose | + | builds acid from |
68381 | 16634 | raffinose | - | builds acid from |
68381 | 17992 | sucrose | + | builds acid from |
68381 | 30849 | L-arabinose | - | builds acid from |
68381 | 18333 | D-arabitol | - | builds acid from |
68381 | 40585 | alpha-cyclodextrin | + | builds acid from |
68381 | 28087 | glycogen | - | builds acid from |
68381 | 27941 | pullulan | - | builds acid from |
68381 | 17306 | maltose | + | builds acid from |
68381 | 28053 | melibiose | - | builds acid from |
68381 | 6731 | melezitose | - | builds acid from |
68381 | 320055 | methyl beta-D-glucopyranoside | + | builds acid from |
68381 | 16443 | D-tagatose | - | builds acid from |
68381 | 16199 | urea | - | hydrolysis |
metabolite production
- @ref: 68381
- Chebi-ID: 15688
- metabolite: acetoin
- production: yes
metabolite tests
@ref | Chebi-ID | metabolite | voges-proskauer-test |
---|---|---|---|
68381 | 15688 | acetoin | + |
120768 | 15688 | acetoin | - |
enzymes
@ref | value | activity | ec |
---|---|---|---|
68382 | alpha-fucosidase | - | 3.2.1.51 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | beta-glucosidase | + | 3.2.1.21 |
68382 | alpha-glucosidase | + | 3.2.1.20 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | beta-galactosidase | - | 3.2.1.23 |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | naphthol-AS-BI-phosphohydrolase | + | |
68382 | acid phosphatase | + | 3.1.3.2 |
68382 | trypsin | - | 3.4.21.4 |
68382 | cystine arylamidase | - | 3.4.11.3 |
68382 | valine arylamidase | - | |
68382 | lipase (C 14) | - | |
68382 | alkaline phosphatase | - | 3.1.3.1 |
68381 | urease | - | 3.5.1.5 |
68381 | beta-mannosidase | + | 3.2.1.25 |
68381 | glycyl tryptophan arylamidase | + | |
68381 | N-acetyl-beta-glucosaminidase | + | 3.2.1.52 |
68381 | pyrrolidonyl arylamidase | + | 3.4.19.3 |
68381 | beta-galactosidase | + | 3.2.1.23 |
68381 | Alanyl-Phenylalanyl-Proline arylamidase | - | |
68381 | alpha-galactosidase | - | 3.2.1.22 |
68381 | beta-glucuronidase | - | 3.2.1.31 |
68381 | beta-glucosidase | + | 3.2.1.21 |
68381 | arginine dihydrolase | - | 3.5.3.6 |
68379 | catalase | + | 1.11.1.6 |
68379 | gelatinase | - | |
68379 | urease | - | 3.5.1.5 |
68379 | beta-glucosidase | + | 3.2.1.21 |
68379 | N-acetyl-beta-glucosaminidase | + | 3.2.1.52 |
68379 | alpha-glucosidase | + | 3.2.1.20 |
68379 | beta-galactosidase | + | 3.2.1.23 |
68379 | beta-glucuronidase | - | 3.2.1.31 |
68379 | alkaline phosphatase | + | 3.1.3.1 |
68379 | pyrrolidonyl arylamidase | + | 3.4.19.3 |
68379 | pyrazinamidase | - | 3.5.1.B15 |
120768 | oxidase | - | |
120768 | alcohol dehydrogenase | - | 1.1.1.1 |
120768 | catalase | - | 1.11.1.6 |
120768 | lysine decarboxylase | - | 4.1.1.18 |
120768 | ornithine decarboxylase | - | 4.1.1.17 |
fatty acid profile
fatty acids
@ref fatty acid percentage ECL 49935 C14:0 8.8 14 49935 C16:0 19.5 16 49935 C18:0 6.7 18 49935 C14:1 ω7c 2.9 13.85 49935 C16:1 ω9c 11.3 15.774 49935 C18:1 ω9c 38.2 17.769 49935 C18:2 ω6,9c/C18:0 ANTE 7 17.724 49935 C20:1 ω9c 1.7 19.77 49935 C20:2 ω6,9c 2.4 19.735 49935 unknown 13.766 1.5 13.766 - type of FA analysis: whole cell analysis
- method/protocol: CCUG
API coryne
@ref | NIT | PYZ | PYRA | PAL | beta GUR | beta GAL | alpha GLU | beta NAG | ESC | URE | GEL | Control | GLU | RIB | XYL | MAN | MAL | LAC | SAC | GLYG | CAT |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
49935 | - | - | + | + | - | + | + | + | + | - | - | - | + | + | - | - | + | + | + | - | + |
API zym
@ref | Control | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
8747 | - | - | +/- | +/- | - | +/- | - | - | - | +/- | + | + | - | - | - | + | + | +/- | - | - |
120768 | - | - | + | + | - | - | - | - | - | - | + | + | - | - | - | + | + | - | - | - |
API rID32STR
@ref | ADH Arg | beta GLU | beta GAR | beta GUR | alpha GAL | PAL | RIB | MAN | SOR | LAC | TRE | RAF | SAC | LARA | DARL | CDEX | VP | APPA | beta GAL | PyrA | beta NAG | GTA | HIP | GLYG | PUL | MAL | MEL | MLZ | Mbeta DG | TAG | beta MAN | URE |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
8747 | - | + | - | - | - | +/- | + | - | - | + | + | - | + | - | - | + | + | - | + | + | + | + | +/- | - | - | + | - | - | + | - | + | - |
Isolation, sampling and environmental information
isolation
@ref | sample type |
---|---|
8747 | milk with malty flavour |
49935 | Raw milk with malty flavour |
67770 | Raw milk with malt flavour |
120768 | Food, Raw milk with malty flavour |
isolation source categories
- Cat1: #Host Body Product
- Cat2: #Fluids
- Cat3: #Milk
taxonmaps
- @ref: 69479
- File name: preview.99_2222.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_13;96_41;97_1423;98_1732;99_2222&stattab=map
- Last taxonomy: Carnobacterium maltaromaticum
- 16S sequence: LC065032
- Sequence Identity:
- Total samples: 12837
- soil counts: 1440
- aquatic counts: 2720
- animal counts: 8257
- plant counts: 420
Safety information
risk assessment
@ref | pathogenicity animal | biosafety level | biosafety level comment |
---|---|---|---|
8747 | yes | 2 | Risk group (German classification) |
120768 | 1 | Risk group (French classification) |
Sequence information
16S sequences
@ref | description | accession | length | database | NCBI tax ID |
---|---|---|---|---|---|
20218 | Carnobacterium maltaromaticum strain ATCC 27865 16S ribosomal RNA gene, partial sequence | GQ304909 | 784 | ena | 2751 |
20218 | Carnobacterium maltaromaticum strain ATCC 27865 16S ribosomal RNA gene, partial sequence | JF749288 | 809 | ena | 2751 |
20218 | Carnobacterium maltaromaticum strain DSM 20342 16S ribosomal RNA gene, partial sequence | M58825 | 1501 | ena | 2751 |
20218 | Lactobacillus maltaromicus gene for 16S ribosomal RNA, partial sequence | D31696 | 195 | ena | 2751 |
20218 | L.maltaromicus 16S ribosomal RNA | X54420 | 1499 | ena | 2751 |
67770 | Carnobacterium maltaromaticum gene for 16S ribosomal RNA, partial sequence, strain: JCM 1154 | LC065032 | 1456 | ena | 2751 |
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Carnobacterium maltaromaticum DSM 20342 MX5 | GCA_000744945 | contig | ncbi | 1449341 |
66792 | Carnobacterium maltaromaticum DSM 20342 | 1449341.4 | wgs | patric | 1449341 |
66792 | Carnobacterium maltaromaticum DSM 20342 strain MX5 | 1449341.3 | wgs | patric | 1449341 |
66792 | Carnobacterium maltaromaticum MX5, DSM 20342 | 2579778520 | draft | img | 2751 |
66792 | Carnobacterium maltaromaticum DSM 20342 | 2700989263 | draft | img | 1449341 |
67770 | Carnobacterium maltaromaticum DSM 20342 | GCA_001437315 | scaffold | ncbi | 1449341 |
GC content
@ref | GC-content |
---|---|
8747 | 36.0 |
67770 | 36 |
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
spore-forming | yes | 88 | no |
motile | no | 92.677 | no |
gram-positive | yes | 92.112 | no |
anaerobic | no | 94.786 | no |
halophile | yes | 72.655 | no |
spore-forming | no | 77.722 | no |
thermophile | no | 99.961 | yes |
glucose-util | yes | 89.037 | no |
aerobic | no | 92.067 | no |
flagellated | no | 95.071 | no |
glucose-ferment | yes | 83.101 | yes |
External links
@ref: 8747
culture collection no.: DSM 20342, ATCC 27865, CCUG 30142, LMG 6903, JCM 1154, BCRC 14054, CECT 4134, CIP 103135, KCTC 3602, NCFB 2382, NCIMB 11966, NRIC 1691, NRRL B-14852, NCDO 2382
straininfo link
- @ref: 71817
- straininfo: 3325
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 11016698 | Numerical phenetic study of the genus Carnobacterium. | Lai S, Manchester LN | Antonie Van Leeuwenhoek | 10.1023/a:1002723609675 | 2000 | *Bacterial Typing Techniques, Cluster Analysis, Enterococcus/classification, Lactobacillaceae/*classification, Lactobacillus/classification, Phenotype | Phenotype |
Phylogeny | 12807185 | Reclassification of Lactobacillus maltaromicus (Miller et al. 1974) DSM 20342(T) and DSM 20344 and Carnobacterium piscicola (Collins et al. 1987) DSM 20730(T) and DSM 20722 as Carnobacterium maltaromaticum comb. nov. | Mora D, Scarpellini M, Franzetti L, Colombo S, Galli A | Int J Syst Evol Microbiol | 10.1099/ijs.0.02405-0 | 2003 | Bacterial Typing Techniques, Carbohydrate Metabolism, DNA, Ribosomal Spacer/analysis, Genotype, Lactobacillaceae/chemistry/*classification/genetics/metabolism, Lactobacillus/chemistry/*classification/genetics/metabolism, Nucleic Acid Hybridization, Phenotype, RNA, Ribosomal, 16S/genetics, RNA, Ribosomal, 23S/genetics, Random Amplified Polymorphic DNA Technique, Restriction Mapping | Metabolism |
Genetics | 20537393 | Defensin of the zebra mussel (Dreissena polymorpha): molecular structure, in vitro expression, antimicrobial activity, and potential functions. | Xu W, Faisal M | Mol Immunol | 10.1016/j.molimm.2010.01.025 | 2010 | Amino Acid Sequence, Animals, Defensins/*chemistry/genetics/pharmacology/physiology, Dreissena/*immunology, Microbial Sensitivity Tests, Molecular Sequence Data, Polymerase Chain Reaction | |
Genetics | 32627213 | Genome-wide comparison of Carnobacterium maltaromaticum derived from diseased fish harbouring important virulence-related genes. | Roh H, Kim BS, Lee MK, Park CI, Kim DH | J Fish Dis | 10.1111/jfd.13208 | 2020 | Animals, Aquaculture, Carnobacterium/*genetics/*pathogenicity, Fish Diseases/*microbiology, Genome, Bacterial, Gram-Positive Bacterial Infections/microbiology/*veterinary, Oncorhynchus mykiss, Republic of Korea, Virulence/*genetics |
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
8747 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20342) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-20342 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
39686 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/14899 | ||||
49935 | Curators of the CCUG | Culture Collection University of Gothenburg (CCUG) (CCUG 30142) | https://www.ccug.se/strain?id=30142 | |||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
68379 | Automatically annotated from API Coryne | |||||
68381 | Automatically annotated from API rID32STR | |||||
68382 | Automatically annotated from API zym | |||||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
71817 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID3325.1 | StrainInfo: A central database for resolving microbial strain identifiers | |||
120768 | Curators of the CIP | Collection of Institut Pasteur (CIP 103135) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103135 |