Strain identifier
BacDive ID: 2225
Type strain: 
Species: Sulfurospirillum arsenophilum
Strain Designation: MIT-13
Strain history: <- E. Falsen <- J. S. Blum, MIT-13 (Geospirillum arsenophilum)
NCBI tax ID(s): 56698 (species)
version 8.1 (current version)
General
@ref: 3998
BacDive-ID: 2225
DSM-Number: 10659
keywords: 16S sequence, Bacteria, anaerobe, mesophilic, Gram-negative, motile, vibrio-shaped
description: Sulfurospirillum arsenophilum MIT-13 is an anaerobe, mesophilic, Gram-negative bacterium that was isolated from anoxic freshwater sediment.
NCBI tax id
- NCBI tax id: 56698
- Matching level: species
strain history
- @ref: 3998
- history: <- E. Falsen <- J. S. Blum, MIT-13 (Geospirillum arsenophilum)
doi: 10.13145/bacdive2225.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Epsilonproteobacteria
- order: Campylobacterales
- family: Campylobacteraceae
- genus: Sulfurospirillum
- species: Sulfurospirillum arsenophilum
- full scientific name: Sulfurospirillum arsenophilum Stolz et al. 1999
@ref: 3998
domain: Bacteria
phylum: Proteobacteria
class: Epsilonproteobacteria
order: Campylobacterales
family: Campylobacteraceae
genus: Sulfurospirillum
species: Sulfurospirillum arsenophilum
full scientific name: Sulfurospirillum arsenophilum Stolz et al. 1999
strain designation: MIT-13
type strain: yes
Morphology
cell morphology
| @ref | gram stain | cell length | cell width | cell shape | motility | flagellum arrangement |
|---|---|---|---|---|---|---|
| 23071 | negative | 1.0-2.0 mm | 0.3 µm | vibrio-shaped | yes | monotrichous, polar |
| 23071 | spiral-shaped |
Culture and growth conditions
culture medium
- @ref: 3998
- name: SULFUROSPIRILLUM BARNESII MEDIUM (DSMZ Medium 771)
- growth: yes
- link: https://mediadive.dsmz.de/medium/771
- composition: Name: SULFUROSPIRILLUM BARNESII MEDIUM (DSMZ Medium 771) Composition: Na-DL-lactate 2.24327 g/l NaNO3 1.69492 g/l Na2CO3 1.49551 g/l Yeast extract 0.997009 g/l NaCl 0.458624 g/l (NH4)2SO4 0.224327 g/l KH2PO4 0.224327 g/l K2HPO4 0.224327 g/l MgSO4 x 7 H2O 0.11665 g/l Na2S x 9 H2O 0.0997009 g/l L-Cysteine HCl x H2O 0.0997009 g/l HCl 0.00249252 g/l FeCl2 x 4 H2O 0.00149551 g/l NaOH 0.000498504 g/l CoCl2 x 6 H2O 0.000189432 g/l MnCl2 x 4 H2O 9.97009e-05 g/l Pyridoxine hydrochloride 9.97009e-05 g/l ZnCl2 6.97906e-05 g/l Calcium D-(+)-pantothenate 4.98504e-05 g/l Riboflavin 4.98504e-05 g/l p-Aminobenzoic acid 4.98504e-05 g/l (DL)-alpha-Lipoic acid 4.98504e-05 g/l Nicotinic acid 4.98504e-05 g/l Thiamine HCl 4.98504e-05 g/l Na2MoO4 x 2 H2O 3.58923e-05 g/l NiCl2 x 6 H2O 2.39282e-05 g/l Biotin 1.99402e-05 g/l Folic acid 1.99402e-05 g/l H3BO3 5.98205e-06 g/l Na2WO4 x 2 H2O 3.98804e-06 g/l Na2SeO3 x 5 H2O 2.99103e-06 g/l CuCl2 x 2 H2O 1.99402e-06 g/l Vitamin B12 9.97009e-07 g/l Distilled water
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 3998 | positive | growth | 30 | mesophilic |
| 23071 | positive | growth | 25.0 | mesophilic |
| 23071 | positive | growth | 30.0 | mesophilic |
| 23071 | no | growth | 42.0 | thermophilic |
| 23071 | positive | optimum | 20.0 | psychrophilic |
culture pH
- @ref: 23071
- ability: positive
- type: optimum
- pH: 7.5
Physiology and metabolism
oxygen tolerance
- @ref: 3998
- oxygen tolerance: anaerobe
halophily
- @ref: 23071
- salt: NaCl
- tested relation: optimum
- concentration: 0.1 %
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 23071 | 15075 | selenate | - | electron acceptor |
| 23071 | 29125 | arsenate | + | electron acceptor |
| 23071 | 33403 | elemental sulfur | + | electron acceptor |
| 23071 | 29806 | fumarate | + | electron acceptor |
| 23071 | 17632 | nitrate | + | electron acceptor |
| 23071 | 16301 | nitrite | + | electron acceptor |
| 23071 | 16094 | thiosulfate | + | electron acceptor |
| 23071 | 15740 | formate | + | electron donor |
| 23071 | 29806 | fumarate | + | electron donor |
| 23071 | 24996 | lactate | + | electron donor |
| 23071 | 15361 | pyruvate | + | electron donor |
| 23071 | 29806 | fumarate | + | fermentation |
| 23071 | 29806 | fumarate | + | growth |
| 23071 | 17632 | nitrate | + | reduction |
Isolation, sampling and environmental information
isolation
| @ref | sample type | geographic location | country | origin.country | continent |
|---|---|---|---|---|---|
| 3998 | anoxic freshwater sediment | Massachusetts, Aberjona watershed | USA | USA | North America |
| 23071 | arsenic-contaminated freshwater sediments |
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Engineered | #Contamination | #Heavy metal |
| #Environmental | #Aquatic | #Freshwater |
| #Environmental | #Aquatic | #Sediment |
taxonmaps
- @ref: 69479
- File name: preview.99_2832.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_283;96_1116;97_1309;98_1939;99_2832&stattab=map
- Last taxonomy: Sulfurospirillum arsenophilum
- 16S sequence: U85964
- Sequence Identity:
- Total samples: 490
- soil counts: 82
- aquatic counts: 398
- animal counts: 7
- plant counts: 3
Safety information
risk assessment
- @ref: 3998
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 3998
- description: Geospirillum arsenophilus 16S ribosomal RNA gene, partial sequence
- accession: U85964
- length: 1321
- database: ena
- NCBI tax ID: 56698
GC content
| @ref | GC-content | method |
|---|---|---|
| 3998 | 40.9 | |
| 23071 | 40.9 | high performance liquid chromatography (HPLC) |
External links
@ref: 3998
culture collection no.: DSM 10659, ATCC 700056
straininfo link
- @ref: 71788
- straininfo: 45396
literature
- topic: Phylogeny
- Pubmed-ID: 10425777
- title: Sulfurospirillum barnesii sp. nov. and Sulfurospirillum arsenophilum sp. nov., new members of the Sulfurospirillum clade of the epsilon Proteobacteria.
- authors: Stolz JF, Ellis DJ, Blum JS, Ahmann D, Lovley DR, Oremland RS
- journal: Int J Syst Bacteriol
- DOI: 10.1099/00207713-49-3-1177
- year: 1999
- mesh: Arsenates/*metabolism, Bacterial Typing Techniques, DNA, Ribosomal/chemistry/genetics, Genes, rRNA, Gram-Negative Bacteria/*classification/cytology/genetics/physiology, Molecular Sequence Data, Oxidation-Reduction, Phylogeny, RNA, Ribosomal, 16S/genetics, Selenic Acid, Selenium Compounds/*metabolism, Sequence Analysis, DNA
- topic2: Metabolism
Reference
| @id | authors | catalogue | doi/url | title | journal | pubmed |
|---|---|---|---|---|---|---|
| 3998 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 10659) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-10659 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 23071 | John F. Stolz,Debra J. Ellis,Jodi Switzer Blum,Dianne Ahmann,Derek R. Lovley,Ronald S. Oremland | 10.1099/00207713-49-3-1177 | Note: Sulfurospirillum barnesii sp. nov. and Sulfurospirillum arsenophilum sp. nov., new members of the Sulfurospirillum clade of the e-Proteobacteria | IJSEM 49: 1177-1180 1999 | 10425777 | |
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
| 71788 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID45396.1 | StrainInfo: A central database for resolving microbial strain identifiers |