Strain identifier
BacDive ID: 218
Type strain:
Species: Trueperella abortisuis
Strain Designation: Murakami
Strain history: CIP <- 2009, S. Murakami, Tokyo Univ. Agriculture, Dept. animal Sci., Atsugi, Japan <- R. Azuma, Higashi-tokura, Kokubunnji, Tokyo, Japan: strain Murakami
NCBI tax ID(s): 445930 (species)
General
@ref: 8143
BacDive-ID: 218
DSM-Number: 19515
keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic, coccus-shaped, colony-forming, animal pathogen
description: Trueperella abortisuis Murakami is an anaerobe, mesophilic, coccus-shaped animal pathogen that forms circular colonies and was isolated from placenta of sow after abortion.
NCBI tax id
- NCBI tax id: 445930
- Matching level: species
strain history
@ref | history |
---|---|
8143 | <- S. Murakami, Tokyo Univ. Agriculture, Dept. Animal Sci., Atsugi, Japan <- R. Azuma; strain Murakami |
67770 | S. Murakami strain Murakami. |
115977 | CIP <- 2009, S. Murakami, Tokyo Univ. Agriculture, Dept. animal Sci., Atsugi, Japan <- R. Azuma, Higashi-tokura, Kokubunnji, Tokyo, Japan: strain Murakami |
doi: 10.13145/bacdive218.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Actinomycetales
- family: Actinomycetaceae
- genus: Trueperella
- species: Trueperella abortisuis
- full scientific name: Trueperella abortisuis (Azuma et al. 2009) Yassin et al. 2011
synonyms
- @ref: 20215
- synonym: Arcanobacterium abortisuis
@ref: 8143
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Actinomycetales
family: Actinomycetaceae
genus: Trueperella
species: Trueperella abortisuis
full scientific name: Trueperella abortisuis (Azuma et al. 2009) Yassin et al. 2011
strain designation: Murakami
type strain: yes
Morphology
cell morphology
@ref | cell length | cell width | cell shape | motility | gram stain |
---|---|---|---|---|---|
22976 | 0.3-2.5 µm | 0.2-0.9 µm | coccus-shaped | no | |
29032 | rod-shaped | positive | |||
115977 | rod-shaped | no | positive |
colony morphology
@ref | type of hemolysis | hemolysis ability | colony size | colony color | colony shape | incubation period |
---|---|---|---|---|---|---|
22976 | beta | 1 | 0.5-1.5 mm | translucent | circular | 2-3 days |
61684 | 2 days |
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
8143 | PYG MEDIUM (MODIFIED) (DSMZ Medium 104) | yes | https://mediadive.dsmz.de/medium/104 | Name: PYG MEDIUM (modified) (DSMZ Medium 104) Composition: Yeast extract 10.0 g/l Peptone 5.0 g/l Trypticase peptone 5.0 g/l Beef extract 5.0 g/l Glucose 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l K2HPO4 0.04 g/l KH2PO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Hemin 0.005 g/l Ethanol 0.0038 g/l Resazurin 0.001 g/l Tween 80 Vitamin K1 NaOH Distilled water |
33644 | MEDIUM 20 - for Anaerobic bacteria | yes | Agar (15.000 g);Glucose (5.000 g);Yeast extract (20.000 g);Tryptone (30.000 g);Cysteine hydrochloride (0.500 g);distilled water (1000.000 ml);Hemin solution -M00149 (25.000 ml) | |
115977 | CIP Medium 20 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=20 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
8143 | positive | growth | 37 | mesophilic |
33644 | positive | growth | 37 | mesophilic |
61684 | positive | growth | 37 | mesophilic |
67770 | positive | growth | 37 | mesophilic |
Physiology and metabolism
oxygen tolerance
@ref | oxygen tolerance |
---|---|
8143 | anaerobe |
29032 | anaerobe |
61684 | microaerophile |
115977 | anaerobe |
spore formation
- @ref: 22976
- spore formation: no
murein
- @ref: 22976
- murein short key: A11.51
- type: A5alpha L-Lys-L-Lys-D-Glu
observation
- @ref: 67770
- observation: quinones: MK-10(H4)
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
22976 | 17924 | D-sorbitol | - | builds acid from |
22976 | 65327 | D-xylose | - | builds acid from |
22976 | 30849 | L-arabinose | - | builds acid from |
22976 | 62345 | L-rhamnose | - | builds acid from |
22976 | 37684 | mannose | - | builds acid from |
22976 | 16634 | raffinose | - | builds acid from |
22976 | 17814 | salicin | - | builds acid from |
22976 | 17992 | sucrose | - | builds acid from |
22976 | 27082 | trehalose | - | builds acid from |
22976 | 5291 | gelatin | - | hydrolysis |
22976 | 17057 | cellobiose | + | builds acid from |
22976 | 17634 | D-glucose | + | builds acid from |
22976 | 16899 | D-mannitol | + | builds acid from |
22976 | 16988 | D-ribose | + | builds acid from |
22976 | 17754 | glycerol | + | builds acid from |
22976 | 28087 | glycogen | + | builds acid from |
22976 | 17716 | lactose | + | builds acid from |
22976 | 17306 | maltose | + | builds acid from |
22976 | 606565 | hippurate | + | hydrolysis |
29032 | 15963 | ribitol | + | carbon source |
29032 | 17057 | cellobiose | + | carbon source |
29032 | 28757 | fructose | + | carbon source |
29032 | 28260 | galactose | + | carbon source |
29032 | 17234 | glucose | + | carbon source |
29032 | 17754 | glycerol | + | carbon source |
29032 | 17716 | lactose | + | carbon source |
29032 | 17306 | maltose | + | carbon source |
29032 | 16634 | raffinose | + | carbon source |
29032 | 17992 | sucrose | + | carbon source |
29032 | 27082 | trehalose | + | carbon source |
29032 | 17632 | nitrate | + | reduction |
115977 | 17632 | nitrate | + | reduction |
115977 | 16301 | nitrite | - | reduction |
68380 | 16199 | urea | - | hydrolysis |
68380 | 29016 | arginine | - | hydrolysis |
68380 | 16024 | D-mannose | - | fermentation |
68380 | 29985 | L-glutamate | - | degradation |
68380 | 17632 | nitrate | - | reduction |
68380 | 27897 | tryptophan | - | energy source |
metabolite production
@ref | Chebi-ID | metabolite | production |
---|---|---|---|
22976 | 15688 | acetoin | yes |
115977 | 35581 | indole | no |
68380 | 35581 | indole | no |
metabolite tests
- @ref: 68380
- Chebi-ID: 35581
- metabolite: indole
- indole test: -
enzymes
@ref | value | activity | ec |
---|---|---|---|
22976 | acid phosphatase | - | 3.1.3.2 |
22976 | alkaline phosphatase | - | 3.1.3.1 |
22976 | alpha-galactosidase | + | 3.2.1.22 |
22976 | alpha-glucosidase | + | 3.2.1.20 |
22976 | arginine dihydrolase | - | 3.5.3.6 |
22976 | beta-galactosidase | + | 3.2.1.23 |
22976 | beta-glucosidase | - | 3.2.1.21 |
22976 | beta-glucuronidase | + | 3.2.1.31 |
22976 | catalase | + | 1.11.1.6 |
22976 | esterase lipase (C 8) | - | |
22976 | leucine arylamidase | + | 3.4.11.1 |
22976 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
22976 | nitrate reductase | - | 1.7.99.4 |
22976 | pyrazinamidase | - | 3.5.1.B15 |
22976 | pyrrolidonyl arylamidase | - | 3.4.19.3 |
22976 | urease | - | 3.5.1.5 |
29032 | alpha-galactosidase | + | 3.2.1.22 |
68382 | alpha-fucosidase | - | 3.2.1.51 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68382 | beta-glucosidase | - | 3.2.1.21 |
68382 | alpha-glucosidase | + | 3.2.1.20 |
68382 | beta-glucuronidase | + | 3.2.1.31 |
68382 | beta-galactosidase | + | 3.2.1.23 |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | naphthol-AS-BI-phosphohydrolase | + | |
68382 | acid phosphatase | + | 3.1.3.2 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | trypsin | - | 3.4.21.4 |
68382 | cystine arylamidase | - | 3.4.11.3 |
68382 | valine arylamidase | - | |
68382 | leucine arylamidase | + | 3.4.11.1 |
68382 | lipase (C 14) | - | |
68382 | esterase lipase (C 8) | - | |
68382 | esterase (C 4) | - | |
68382 | alkaline phosphatase | - | 3.1.3.1 |
68380 | serine arylamidase | - | |
68380 | glutamyl-glutamate arylamidase | - | |
68380 | histidine arylamidase | - | |
68380 | glycin arylamidase | - | |
68380 | alanine arylamidase | - | 3.4.11.2 |
68380 | tyrosine arylamidase | - | |
68380 | pyrrolidonyl arylamidase | - | 3.4.19.3 |
68380 | leucyl glycin arylamidase | - | 3.4.11.1 |
68380 | proline-arylamidase | + | 3.4.11.5 |
68380 | alkaline phosphatase | - | 3.1.3.1 |
68380 | tryptophan deaminase | - | 4.1.99.1 |
68380 | alpha-fucosidase | - | 3.2.1.51 |
68380 | glutamate decarboxylase | - | 4.1.1.15 |
68380 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68380 | beta-glucuronidase | + | 3.2.1.31 |
68380 | alpha-arabinosidase | - | 3.2.1.55 |
68380 | beta-glucosidase | - | 3.2.1.21 |
68380 | alpha-glucosidase | + | 3.2.1.20 |
68380 | beta-Galactosidase 6-phosphate | - | |
68380 | beta-galactosidase | + | 3.2.1.23 |
68380 | arginine dihydrolase | - | 3.5.3.6 |
68380 | urease | - | 3.5.1.5 |
115977 | oxidase | - | |
115977 | catalase | - | 1.11.1.6 |
115977 | urease | - | 3.5.1.5 |
API zym
@ref | Control | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
115977 | - | - | - | - | - | + | - | - | - | - | + | + | - | + | + | + | - | - | - | - |
API rID32A
@ref | URE | ADH Arg | alpha GAL | beta GAL | beta GP | alpha GLU | beta GLU | alpha ARA | beta GUR | beta NAG | MNE | RAF | GDC | alpha FUC | NIT | IND | PAL | ArgA | ProA | LGA | PheA | LeuA | PyrA | TyrA | AlaA | GlyA | HisA | GGA | SerA |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
8143 | - | - | +/- | + | - | + | - | - | + | - | - | + | - | - | - | - | - | +/- | + | - | +/- | +/- | - | - | - | - | - | - | - |
8143 | - | - | - | + | - | + | - | - | + | - | - | - | - | - | - | - | - | - | + | - | - | - | - | - | - | - | - | - | - |
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent | sampling date |
---|---|---|---|---|---|---|
8143 | placenta of sow after abortion | Chiba Prefecture | Japan | JPN | Asia | |
61684 | Porcine placenta,abortion | Japan | JPN | Asia | 2000 | |
67770 | Sow's placenta after an abortion | |||||
115977 | Sow, placenta following an abortion |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Host | #Mammals | #Suidae (Pig,Swine) |
#Host Body-Site | #Urogenital tract | |
#Host Body Product | #Urogenital tract | #Abort |
Safety information
risk assessment
@ref | pathogenicity animal | biosafety level | biosafety level comment |
---|---|---|---|
8143 | yes | 2 | Risk group (German classification) |
115977 | 1 | Risk group (French classification) |
Sequence information
16S sequences
@ref | description | accession | length | database | NCBI tax ID |
---|---|---|---|---|---|
20218 | Arcanobacterium abortisuis partial 16S rRNA gene, 16S-23S IGS and partial 23S rRNA IGS, strain DSM 19515 | FN667627 | 468 | ena | 445930 |
8143 | Arcanobacterium abortisuis gene for 16S rRNA, partial sequence | AB305159 | 1519 | ena | 445930 |
Genome sequences
- @ref: 66792
- description: Trueperella abortisuis DSM 19515
- accession: 2923491830
- assembly level: draft
- database: img
- NCBI tax ID: 445930
GC content
@ref | GC-content | method |
---|---|---|
8143 | 63.8 | high performance liquid chromatography (HPLC) |
29032 | 63.8 |
External links
@ref: 8143
culture collection no.: DSM 19515, ATCC BAA 1522, JCM 14813, CCUG 58417, CIP 110059, KCTC 15151
straininfo link
- @ref: 69901
- straininfo: 362773
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 19502337 | Arcanobacterium abortisuis sp. nov., isolated from a placenta of a sow following an abortion. | Azuma R, Murakami S, Ogawa A, Okada Y, Miyazaki S, Makino T | Int J Syst Evol Microbiol | 10.1099/ijs.0.004465-0 | 2009 | *Abortion, Veterinary, Actinomycetales Infections/microbiology/*veterinary, Animals, Arcanobacterium/*classification/genetics/isolation & purification/physiology, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, Female, Molecular Sequence Data, Phenotype, Phylogeny, Placenta/*microbiology, Polymerase Chain Reaction/methods, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Species Specificity, Swine, Swine Diseases/*microbiology | Genetics |
Phylogeny | 20926209 | Characterization of Arcanobacterium abortisuis by phenotypic properties and by sequencing the 16S-23S rDNA intergenic spacer region. | Ulbegi-Mohyla H, Hassan AA, Hijazin M, Alber J, Lammler C, Abdulmawjood A, Prenger-Berninghoff E, Weiss R, Zschock M | Vet Microbiol | 10.1016/j.vetmic.2010.08.026 | 2010 | Animals, Arcanobacterium/classification/*genetics/isolation & purification, DNA, Bacterial/genetics, DNA, Intergenic/*genetics, DNA, Ribosomal/genetics, Genotype, Phenotype, RNA, Ribosomal, 16S/genetics, RNA, Ribosomal, 23S/genetics, Sequence Analysis, DNA, Swine/microbiology | Genetics |
Phylogeny | 35131429 | Development of a loop-mediated isothermal amplification (LAMP) assay for molecular identification of Trueperella abortisuis isolated from pigs. | Ahmed MFE, Alssahen M, Lammler C, Foster G, Kreitlow A, Hennig-Pauka I, Plotz M, Abdulmawjood A | Mol Cell Probes | 10.1016/j.mcp.2022.101795 | 2022 | Actinomycetaceae, Animals, *Arcanobacterium/genetics, Female, Male, Molecular Diagnostic Techniques, *Nucleic Acid Amplification Techniques, RNA, Ribosomal, 16S/genetics, Sensitivity and Specificity, Swine |
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed | ID_cross_reference |
---|---|---|---|---|---|---|---|
8143 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 19515) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-19515 | ||||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | ||||
20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | ||
22976 | A. F. Yassin,H. Hupfer,C. Siering,P. Schumann | 10.1099/ijs.0.020032-0 | Comparative chemotaxonomic and phylogenetic studies on the genus Arcanobacterium Collins et al. 1982 emend. Lehnen et al. 2006: proposal for Trueperella gen. nov. and emended description of the genus Arcanobacterium | IJSEM 61: 1265-1274 2011 | 20622055 | ||
29032 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | 10.1128/mSphere.00237-17 | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 28776041 | 25462 | ||
33644 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/7874 | |||||
61684 | Curators of the CCUG | Culture Collection University of Gothenburg (CCUG) (CCUG 58417) | https://www.ccug.se/strain?id=58417 | ||||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | ||||
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | |||||
68380 | Automatically annotated from API rID32A | ||||||
68382 | Automatically annotated from API zym | ||||||
69901 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID362773.1 | StrainInfo: A central database for resolving microbial strain identifiers | ||||
115977 | Curators of the CIP | Collection of Institut Pasteur (CIP 110059) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20110059 |