Strain identifier

BacDive ID: 217

Type strain: Yes

Species: Trueperella bonasi

Strain Designation: 2W106/04, W106/04

Strain history: CIP <- 2006, DSMZ <- A. Lehnen, Inst. für Zoo, Berlin, Germany: strain W106/04

NCBI tax ID(s): 312286 (species)

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General

@ref: 6798

BacDive-ID: 217

DSM-Number: 17163

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, coccus-shaped, colony-forming, animal pathogen

description: Trueperella bonasi 2W106/04 is a microaerophile, mesophilic, coccus-shaped animal pathogen that forms circular colonies and was isolated from prepuce of European bison Bison bonasus suffering from balanoposthitis.

NCBI tax id

  • NCBI tax id: 312286
  • Matching level: species

strain history

@refhistory
6798<- A. Lehnen; W106/04
116838CIP <- 2006, DSMZ <- A. Lehnen, Inst. für Zoo, Berlin, Germany: strain W106/04

doi: 10.13145/bacdive217.20241212.9.2

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Actinomycetales
  • family: Actinomycetaceae
  • genus: Trueperella
  • species: Trueperella bonasi
  • full scientific name: Trueperella bonasi (Lehnen et al. 2006) Yassin et al. 2011

  • synonyms

    • @ref: 20215
    • synonym: Arcanobacterium bonasi

@ref: 6798

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Actinomycetaceae

genus: Trueperella

species: Trueperella bonasi

full scientific name: Trueperella bonasi (Lehnen et al. 2006) Yassin et al. 2011

strain designation: 2W106/04, W106/04

type strain: yes

Morphology

cell morphology

@refcell lengthcell widthcell shapemotilitygram stain
229760.3-2.5 µm0.2-0.9 µmcoccus-shapedno
31626rod-shapednopositive
116838oval-shapednopositive

colony morphology

@reftype of hemolysishemolysis abilitycolony sizecolony colorcolony shapeincubation period
22976beta10.5-1.5 mmtranslucentcircular2-3 days
621372-3 days

pigmentation

  • @ref: 31626
  • production: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
6798COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
40027MEDIUM 45 - for Columbia agar with sheep bloodyesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Sheepblood (100.000 ml)
116838CIP Medium 45yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=45

culture temp

@refgrowthtypetemperature
6798positivegrowth37
31626positivegrowth37
31626positiveoptimum37
40027positivegrowth37
62137positivegrowth37

culture pH

@refabilitytypepH
31626positivegrowth7
31626positiveoptimum7

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
6798microaerophile
6798aerobe
31626facultative anaerobe
62137microaerophile

spore formation

@refspore formation
22976no
31626no

murein

  • @ref: 22976
  • murein short key: A11.51
  • type: A5alpha L-Lys-L-Lys-D-Glu

observation

  • @ref: 31626
  • observation: aggregates in chains

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2297617634D-glucose-builds acid from
2297616899D-mannitol-builds acid from
2297616988D-ribose-builds acid from
2297617924D-sorbitol-builds acid from
2297665327D-xylose-builds acid from
2297617754glycerol-builds acid from
2297628087glycogen-builds acid from
2297630849L-arabinose-builds acid from
2297662345L-rhamnose-builds acid from
2297617716lactose-builds acid from
2297617306maltose-builds acid from
2297637684mannose-builds acid from
2297616634raffinose-builds acid from
2297617814salicin-builds acid from
2297617992sucrose-builds acid from
2297627082trehalose-builds acid from
229765291gelatin-hydrolysis
22976606565hippurate+hydrolysis
3162617754glycerol+carbon source
3162617716lactose+carbon source
3162617306maltose+carbon source
6838027897tryptophan-energy source
6838029985L-glutamate-degradation
6838016634raffinose-fermentation
6838029016arginine-hydrolysis
6838016024D-mannose-fermentation
6838016199urea-hydrolysis
11683817632nitrate-reduction
11683816301nitrite-reduction
6838017632nitrate-reduction

metabolite production

@refChebi-IDmetaboliteproduction
2297615688acetoinno
6838035581indoleno
11683835581indoleno

metabolite tests

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
22976acid phosphatase+3.1.3.2
22976alkaline phosphatase-3.1.3.1
22976alpha-galactosidase-3.2.1.22
22976alpha-glucosidase-3.2.1.20
22976arginine dihydrolase-3.5.3.6
22976beta-galactosidase-3.2.1.23
22976beta-glucosidase-3.2.1.21
22976beta-glucuronidase+3.2.1.31
22976catalase-1.11.1.6
22976esterase lipase (C 8)+
22976leucine arylamidase-3.4.11.1
22976N-acetyl-beta-glucosaminidase-3.2.1.52
22976nitrate reductase-1.7.99.4
22976pyrazinamidase+3.5.1.B15
22976pyrrolidonyl arylamidase+3.4.19.3
22976urease-3.5.1.5
31626catalase+1.11.1.6
68382alpha-fucosidase-3.2.1.51
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382beta-glucosidase-3.2.1.21
68382beta-glucuronidase+3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382acid phosphatase-3.1.3.2
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382esterase lipase (C 8)+
68382alkaline phosphatase-3.1.3.1
68382esterase (C 4)+
116838oxidase-
116838catalase-1.11.1.6
116838urease-3.5.1.5
68380serine arylamidase-
68380glutamyl-glutamate arylamidase-
68380glycin arylamidase+
68380tyrosine arylamidase-
68380leucine arylamidase+3.4.11.1
68380phenylalanine arylamidase-
68380glutamate decarboxylase-4.1.1.15
68380N-acetyl-beta-glucosaminidase-3.2.1.52
68380beta-glucuronidase+3.2.1.31
68380alpha-arabinosidase-3.2.1.55
68380beta-Galactosidase 6-phosphate-
68380beta-galactosidase-3.2.1.23
68380alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase-
68380histidine arylamidase+
68380alanine arylamidase+3.4.11.2
68380pyrrolidonyl arylamidase+3.4.19.3
68380leucyl glycin arylamidase+3.4.11.1
68380proline-arylamidase-3.4.11.5
68380L-arginine arylamidase+
68380alkaline phosphatase-3.1.3.1
68380tryptophan deaminase-4.1.99.1
68380alpha-fucosidase-3.2.1.51
68380beta-glucosidase-3.2.1.21
68380alpha-glucosidase-3.2.1.20
68380arginine dihydrolase-3.5.3.6
68380urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
116838--++-+--------+-----

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
6798--------+--------+-+-++-+++--

Isolation, sampling and environmental information

isolation

@refsample typehost speciesgeographic locationcountryorigin.countrycontinent
6798prepuce of European bison Bison bonasus suffering from balanoposthitisBison bonasusBialowieza National ParkPolandPOLEurope
62137Prepuce of European bisonPolandPOLEurope
116838European bison, prepucePolandPOLEurope

isolation source categories

Cat1Cat2Cat3
#Host#Mammals#Bovinae (Cow, Cattle)
#Host Body-Site#Urogenital tract

taxonmaps

  • @ref: 69479
  • File name: preview.99_129776.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_28;96_50571;97_65116;98_86895;99_129776&stattab=map
  • Last taxonomy: Trueperella bonasi subclade
  • 16S sequence: AJ879697
  • Sequence Identity:
  • Total samples: 115
  • soil counts: 14
  • aquatic counts: 4
  • animal counts: 97

Safety information

risk assessment

@refpathogenicity animalbiosafety levelbiosafety level comment
6798yes2Risk group (German classification)
1168381Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Arcanobacterium bonasi strain DSM 17163 16S ribosomal RNA gene and 16S-23S ribosomal RNA intergenic spacer, partial sequenceEU194570512nuccore312286
6798Arcanobacterium bonasi 16S rRNA gene, type strain: 2(W106/04)TAJ8796971445nuccore312286

Genome sequences

  • @ref: 66792
  • description: Trueperella bonasi DSM 17163
  • accession: 2923644855
  • assembly level: draft
  • database: img
  • NCBI tax ID: 312286

GC content

  • @ref: 6798
  • GC-content: 58.1
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 6798

culture collection no.: CCUG 59861, DSM 17163, CIP 109232, NCTC 13355

straininfo link

  • @ref: 69900
  • straininfo: 290959

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny16585707Arcanobacterium bialowiezense sp. nov. and Arcanobacterium bonasi sp. nov., isolated from the prepuce of European bison bulls (Bison bonasus) suffering from balanoposthitis, and emended description of the genus Arcanobacterium Collins et al. 1983.Lehnen A, Busse HJ, Frolich K, Krasinska M, Kampfer P, Speck SInt J Syst Evol Microbiol10.1099/ijs.0.63923-02006Actinomycetaceae/chemistry/*classification/genetics/isolation & purification, Actinomycetales Infections/*microbiology, Animals, Bacterial Typing Techniques, Bison/microbiology, Male, Molecular Sequence Data, Penis/microbiology, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/analysis/geneticsGenetics
Phylogeny18374522Molecular identification of Arcanobacterium bialowiezense and Arcanobacterium bonasi based on 16S-23S rRNA intergenic spacer region sequences.Hassan AA, Mohyla H, Kanbar T, Alber J, Lammler C, Abdulmawjood A, Speck S, Zschock M, Weiss RVet Microbiol10.1016/j.vetmic.2008.02.0082008Actinomycetaceae/*genetics, DNA, Ribosomal Spacer/*genetics, Phylogeny, RNA, Bacterial/*genetics, RNA, Ribosomal/*genetics, RNA, Ribosomal, 16S/*genetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmedID_cross_reference
6798Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 17163)https://www.dsmz.de/collection/catalogue/details/culture/DSM-17163
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
22976A. F. Yassin,H. Hupfer,C. Siering,P. Schumann10.1099/ijs.0.020032-0Comparative chemotaxonomic and phylogenetic studies on the genus Arcanobacterium Collins et al. 1982 emend. Lehnen et al. 2006: proposal for Trueperella gen. nov. and emended description of the genus ArcanobacteriumIJSEM 61: 1265-1274 201120622055
31626Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2877604127911
40027Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/6951
62137Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 59861)https://www.ccug.se/strain?id=59861
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68380Automatically annotated from API rID32A
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69900Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID290959.1StrainInfo: A central database for resolving microbial strain identifiers
116838Curators of the CIPCollection of Institut Pasteur (CIP 109232)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20109232