Strain identifier

BacDive ID: 2130

Type strain: Yes

Species: Campylobacter sputorum

Strain Designation: S-17

Strain history: CIP <- 1994, CCUG <- 1980, L.V. Holdeman, VPI: strain S-17

NCBI tax ID(s): 206 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 3970

BacDive-ID: 2130

DSM-Number: 10535

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, rod-shaped

description: Campylobacter sputorum S-17 is a microaerophile, mesophilic, rod-shaped bacterium that was isolated from human oral cavity.

NCBI tax id

  • NCBI tax id: 206
  • Matching level: species

strain history

@refhistory
3970<- CCUG <- L.V. Holdeman, VPI USA; S-17
67771<- CCUG <- LV Holdeman, VPI S-17
123286CIP <- 1994, CCUG <- 1980, L.V. Holdeman, VPI: strain S-17

doi: 10.13145/bacdive2130.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Epsilonproteobacteria
  • order: Campylobacterales
  • family: Campylobacteraceae
  • genus: Campylobacter
  • species: Campylobacter sputorum
  • full scientific name: Campylobacter sputorum (Prévot 1940) Véron and Chatelain 1973 (Approved Lists 1980)
  • synonyms

    • @ref: 20215
    • synonym: Vibrio sputorum

@ref: 3970

domain: Bacteria

phylum: Proteobacteria

class: Epsilonproteobacteria

order: Campylobacterales

family: Campylobacteraceae

genus: Campylobacter

species: Campylobacter sputorum subsp. sputorum

full scientific name: Campylobacter sputorum subsp. sputorum (Prévot 1940) Véron and Chatelain 1973 emend. Roop et al. 1986

strain designation: S-17

type strain: yes

Morphology

cell morphology

@refcell shapegram stainmotility
67771rod-shaped
67771negative
123286rod-shapednegativeyes

colony morphology

@refincubation period
452332 days
123286

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
3970SEMISOLID BRUCELLA BROTH (DSMZ Medium 703)yeshttps://mediadive.dsmz.de/medium/703Name: SEMISOLID BRUCELLA BROTH (DSMZ Medium 703) Composition: Casein peptone 10.0 g/l Bacto peptone 10.0 g/l NaCl 5.0 g/l Yeast extract 2.0 g/l Agar 1.6 g/l D-Glucose 1.0 g/l Distilled water
40777MEDIUM 24 - for Campylobacter concisus, C. sputorum and C. mucosalisyesDistilled water make up to (1000.000 ml);Horse blood (100.000 ml);Brainheart infusion agar (52.000 g);Formate solution - M00175 (100.000 ml);Fumaric acid solution- M0176 (100.000 ml)
123286CIP Medium 24yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=24

culture temp

@refgrowthtypetemperaturerange
3970positivegrowth37mesophilic
40777positivegrowth37mesophilic
45233positivegrowth37mesophilic
67771positivegrowth37mesophilic
123286positivegrowth25-41
123286nogrowth5psychrophilic
123286nogrowth10psychrophilic
123286nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
3970microaerophile
45233microaerophile
67771anaerobe

halophily

  • @ref: 123286
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 3.5 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
123286606565hippurate+hydrolysis
12328617632nitrate+reduction
12328616301nitrite+reduction
6837317632nitrate-reduction
6837317634D-glucose-assimilation
6837330031succinate-assimilation
6837330089acetate-assimilation
6837317272propionate-assimilation
6837325115malate-assimilation
6837316947citrate-assimilation

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitivesensitivity conc.
6837348923erythromycinyesyes14 µg
68373474053cefazolinyesyes224 µg
68373100147nalidixic acidyesyes84 µg

metabolite production

@refChebi-IDmetaboliteproduction
6837316136hydrogen sulfideyes
12328635581indoleno

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase-3.1.3.1
68373alkaline phosphatase-3.1.3.1
68373L-arginine arylamidase-
68373pyrrolidonyl arylamidase-3.4.19.3
68373gamma-glutamyltransferase-2.3.2.2
123286oxidase+
123286gelatinase-
123286catalase-1.11.1.6
123286gamma-glutamyltransferase-2.3.2.2
123286urease-3.5.1.5

fatty acid profile

  • fatty acids

    @reffatty acidpercentageECL
    45233C12:00.512
    45233C14:022.114
    45233C16:026.716
    45233C18:00.518
    45233C12:0 ALDE ?1.310.915
    45233C13:0 ISO 2OH0.513.814
    45233C13:1 at 12-131.912.931
    45233C14:0 3OH/C16:1 ISO I4.315.485
    45233C16:0 3OH317.52
    45233C16:1 ω7c8.115.819
    45233C18:1 ω7c /12t/9t28.917.824
    45233C18:2 ω6,9c/C18:0 ANTE1.617.724
    45233unknown 14.5030.614.503
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
123286--++-+----++---+----

API CAM

@refURENITESTHIPGGTTTCPYRAArgAAspAPALH2SGLUSUTNALCFZACEPROPMLTCITEROCAT
3970+--+-+/---+-+---------+
45233--+-------+----------

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinent
3970human oral cavityUSAUSANorth America
45233Human oral cavityUSAUSANorth America
67771From human oral cavityUSAUSANorth America
123286Human, Oral cavityBrazilBRAMiddle and South America

isolation source categories

Cat1Cat2Cat3
#Host#Human
#Host Body-Site#Oral cavity and airways
#Host Body-Site#Oral cavity and airways#Mouth

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
39702Risk group (German classification)
1232862Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Campylobacter sputorum strain ATCC 33562 16S ribosomal RNA gene, partial sequenceJF7476101469ena206
20218Campylobacter sputorum biovar sputorum strain ATCC 35980 16S ribosomal RNA gene, partial sequenceJX9125261434ena32024
20218Campylobacter sputorum strain LMG 7795 16S ribosomal RNA (rrs) gene, partial sequenceDQ1741491572ena206
20218C.sputorum sputorum (LMG 7795) 16S rRNA geneX677751695ena32024
67771Campylobacter sputorum biovar sputorum strain biovar sputorum;LMG 7795 16S ribosomal RNA, partial sequenceNR_0448391695nuccore32024

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Campylobacter sputorum subsp. sputorum LMG 7795GCA_008245005completencbi32024
66792Campylobacter sputorum subsp. sputorum CCUG 9728GCA_008802035contigncbi32024
66792Campylobacter sputorum biovar sputorum strain CCUG 972832024.7wgspatric32024
66792Campylobacter sputorum biovar sputorum strain LMG 779532024.6completepatric32024

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileyes87.303no
flagellatedyes57.671no
gram-positiveno98.819no
anaerobicyes52.861no
aerobicno97.106yes
halophileno80.705no
spore-formingno95.665no
glucose-utilno88.812no
thermophileno60.575yes
glucose-fermentno85.453no

External links

@ref: 3970

culture collection no.: LMG 7795, LMG 6450, KCTC 15215, DSM 10535, ATCC 35980, CCUG 9728, CIP 103749, NCTC 11528, KCTC 2682, VPI S-17

straininfo link

  • @ref: 71758
  • straininfo: 1563

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
3970Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 10535)https://www.dsmz.de/collection/catalogue/details/culture/DSM-10535
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
40777Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/15581
45233Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 9728)https://www.ccug.se/strain?id=9728
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
68373Automatically annotated from API CAM
68382Automatically annotated from API zym
71758Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID1563.1StrainInfo: A central database for resolving microbial strain identifiers
123286Curators of the CIPCollection of Institut Pasteur (CIP 103749)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103749