Strain identifier

BacDive ID: 212

Type strain: Yes

Species: Trueperella pyogenes

Strain Designation: 84, C-100, 84 (C.100)

Strain history: CIP <- 1988, NCTC, Corynebacterium pyogenes <- H.R. Carne: strain 84 (C.100)

NCBI tax ID(s): 1661 (species)

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General

@ref: 8977

BacDive-ID: 212

DSM-Number: 20630

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic, coccus-shaped, colony-forming, human pathogen

description: Trueperella pyogenes 84 is an anaerobe, mesophilic, coccus-shaped human pathogen that forms circular colonies and was isolated from pig.

NCBI tax id

  • NCBI tax id: 1661
  • Matching level: species

strain history

@refhistory
8977<- NCTC; NCTC 5224 <- H.R. Carne; 84
122084CIP <- 1988, NCTC, Corynebacterium pyogenes <- H.R. Carne: strain 84 (C.100)

doi: 10.13145/bacdive212.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Actinomycetales
  • family: Actinomycetaceae
  • genus: Trueperella
  • species: Trueperella pyogenes
  • full scientific name: Trueperella pyogenes (Glage 1903) Yassin et al. 2011

  • synonyms

    @refsynonym
    20215Actinomyces pyogenes
    20215Arcanobacterium pyogenes
    20215Corynebacterium pyogenes
    20215Bacillus pyogenes

@ref: 8977

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Actinomycetaceae

genus: Trueperella

species: Trueperella pyogenes

full scientific name: Trueperella pyogenes (Glage 1903) Yassin et al. 2011

strain designation: 84, C-100, 84 (C.100)

type strain: yes

Morphology

cell morphology

  • @ref: 22976
  • cell length: 0.3-2.5 µm
  • cell width: 0.2-0.9 µm
  • cell shape: coccus-shaped
  • motility: no

colony morphology

  • @ref: 22976
  • type of hemolysis: beta
  • hemolysis ability: 1
  • colony size: 0.5-1.5 mm
  • colony color: translucent
  • colony shape: circular
  • incubation period: 2-3 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
8977COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium693.pdf
40534MEDIUM 6 - Columbia agar with 10 % horse bloodyesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml)
8977PYG MEDIUM (MODIFIED) (DSMZ Medium 104)yeshttps://mediadive.dsmz.de/medium/104Name: PYG MEDIUM (modified) (DSMZ Medium 104) Composition: Yeast extract 10.0 g/l Peptone 5.0 g/l Trypticase peptone 5.0 g/l Beef extract 5.0 g/l Glucose 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l K2HPO4 0.04 g/l KH2PO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Hemin 0.005 g/l Ethanol 0.0038 g/l Resazurin 0.001 g/l Tween 80 Vitamin K1 NaOH Distilled water
122084CIP Medium 6yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=6

culture temp

@refgrowthtypetemperaturerange
40534positivegrowth37mesophilic
8977positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 8977
  • oxygen tolerance: anaerobe

spore formation

  • @ref: 22976
  • spore formation: no

murein

  • @ref: 8977
  • murein short key: A11.53
  • type: A5alpha L-Lys-L-Ala-L-Lys-D-Glu

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2297617057cellobiose-builds acid from
2297616899D-mannitol-builds acid from
2297617924D-sorbitol-builds acid from
2297617754glycerol-builds acid from
2297630849L-arabinose-builds acid from
2297662345L-rhamnose-builds acid from
2297616634raffinose-builds acid from
2297617814salicin-builds acid from
2297617992sucrose-builds acid from
2297617634D-glucose+builds acid from
2297616988D-ribose+builds acid from
2297665327D-xylose+builds acid from
2297628087glycogen+builds acid from
2297617716lactose+builds acid from
2297617306maltose+builds acid from
2297637684mannose+builds acid from
2297627082trehalose+builds acid from
229765291gelatin+hydrolysis
22976606565hippurate+hydrolysis
683794853esculin-hydrolysis
6837916199urea-hydrolysis
683795291gelatin+hydrolysis
6837916988D-ribose+fermentation
6837965327D-xylose+fermentation
6837917306maltose+fermentation
6837917716lactose+fermentation
6838016199urea-hydrolysis
6838029016arginine-hydrolysis
6838016024D-mannose-fermentation
6838016634raffinose-fermentation
6838029985L-glutamate-degradation
6838017632nitrate-reduction
6838027897tryptophan-energy source

metabolite production

@refChebi-IDmetaboliteproduction
2297615688acetoinno
6838035581indoleno

metabolite tests

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
22976acid phosphatase-3.1.3.2
22976alkaline phosphatase-3.1.3.1
22976alpha-galactosidase-3.2.1.22
22976alpha-glucosidase+3.2.1.20
22976arginine dihydrolase-3.5.3.6
22976beta-galactosidase+3.2.1.23
22976beta-glucosidase-3.2.1.21
22976beta-glucuronidase+3.2.1.31
22976catalase-1.11.1.6
22976esterase lipase (C 8)-
22976leucine arylamidase+3.4.11.1
22976N-acetyl-beta-glucosaminidase-3.2.1.52
22976nitrate reductase-1.7.99.4
22976pyrazinamidase-3.5.1.B15
22976pyrrolidonyl arylamidase-3.4.19.3
22976urease-3.5.1.5
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase+3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase-
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
68380glutamyl-glutamate arylamidase-
68380histidine arylamidase-
68380proline-arylamidase+3.4.11.5
68380alkaline phosphatase-3.1.3.1
68380tryptophan deaminase-4.1.99.1
68380alpha-fucosidase-3.2.1.51
68380glutamate decarboxylase-4.1.1.15
68380N-acetyl-beta-glucosaminidase-3.2.1.52
68380alpha-arabinosidase-3.2.1.55
68380beta-glucosidase-3.2.1.21
68380beta-Galactosidase 6-phosphate-
68380alpha-galactosidase-3.2.1.22
68380arginine dihydrolase-3.5.3.6
68380urease-3.5.1.5
68379catalase-1.11.1.6
68379gelatinase+
68379urease-3.5.1.5
68379beta-glucosidase-3.2.1.21
68379N-acetyl-beta-glucosaminidase-3.2.1.52
68379alpha-glucosidase+3.2.1.20
68379beta-galactosidase+3.2.1.23
68379beta-glucuronidase+3.2.1.31
68379alkaline phosphatase+3.1.3.1
68379pyrrolidonyl arylamidase+3.4.19.3
68379pyrazinamidase-3.5.1.B15

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELControlRIBXYLMANMALLACSACGLYGCAT
45920--+++++---+-++-++---
45920+-+++++---+-+++++++-

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
45920-+++-+----+--+++----

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
8977---+/--+/---+--------+/-+------+/----
8977---+-+-----------+++/-+/-+/-+/-+/-++--+
8977---+-+--+--------++------+--+/-

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation date
8977pig
22976pyogenic disease conditions in many species of domestic animals and in humans
45920Pig
122084Animal, PigSydney, New South WalesAustraliaAUSAustralia and Oceania1937

isolation source categories

Cat1Cat2
#Host#Mammals
#Infection#Disease
#Host
#Host#Human

taxonmaps

  • @ref: 69479
  • File name: preview.99_3661.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_28;96_1048;97_1225;98_2800;99_3661&stattab=map
  • Last taxonomy: Trueperella pyogenes subclade
  • 16S sequence: X79225
  • Sequence Identity:
  • Total samples: 14544
  • soil counts: 1014
  • aquatic counts: 649
  • animal counts: 12702
  • plant counts: 179

Safety information

risk assessment

@refpathogenicity humanpathogenicity animalbiosafety levelbiosafety level comment
8977yesyes2Risk group (German classification)
1220842Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Arcanobacterium pyogenes partial 16S rRNA gene, strain LMG-16162AJ298640389ena1661
20218Arcanobacterium pyogenes strain ATCC 19411 16S ribosomal RNA gene, partial sequenceGU5855781293ena1661
20218A.pyogenes 16S ribosomal RNAM295521361ena1661
8977A.pyogenes (NCTC 5224) 16S rRNA geneX792251323ena1661

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Trueperella pyogenes NCTC5224GCA_900460345contigncbi1661
66792Trueperella pyogenes DSM 20630GCA_014191445contigncbi1661
66792Trueperella pyogenes strain DSM 206301661.79wgspatric1661
66792Trueperella pyogenes strain NCTC52241661.41wgspatric1661
66792Trueperella pyogenes NCTC 52242835018212draftimg1661
66792Trueperella pyogenes DSM 206302824226513draftimg1661

GC content

@refGC-contentmethod
897756
2297656-58high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileno93.537yes
gram-positiveyes92.788no
anaerobicno56.217yes
aerobicno90.656yes
halophileno85.85no
spore-formingno95.924yes
thermophileno90.266yes
glucose-utilyes85.173no
flagellatedno97.259no
glucose-fermentyes80.205no

External links

@ref: 8977

culture collection no.: DSM 20630, ATCC 19411, CCUG 13230, CIP 103129, LMG 16162, NCTC 5224

straininfo link

  • @ref: 69895
  • straininfo: 8153

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Enzymology2684205Isolation and characterization of an extracellular protease of Actinomyces pyogenes.Schaufuss P, Sting R, Lammler CZentralbl Bakteriol10.1016/s0934-8840(89)80104-51989Actinomyces/*enzymology, Animals, Caseins/metabolism, Chromatography, DEAE-Cellulose, Chromatography, High Pressure Liquid, Electrophoresis, Polyacrylamide Gel, Humans, Hydrogen-Ion Concentration, Isoelectric Focusing, Kinetics, Molecular Weight, Peptide Hydrolases/analysis/*isolation & purification, Protease Inhibitors/pharmacology, TemperaturePhylogeny
Phylogeny16585707Arcanobacterium bialowiezense sp. nov. and Arcanobacterium bonasi sp. nov., isolated from the prepuce of European bison bulls (Bison bonasus) suffering from balanoposthitis, and emended description of the genus Arcanobacterium Collins et al. 1983.Lehnen A, Busse HJ, Frolich K, Krasinska M, Kampfer P, Speck SInt J Syst Evol Microbiol10.1099/ijs.0.63923-02006Actinomycetaceae/chemistry/*classification/genetics/isolation & purification, Actinomycetales Infections/*microbiology, Animals, Bacterial Typing Techniques, Bison/microbiology, Male, Molecular Sequence Data, Penis/microbiology, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/analysis/geneticsGenetics
Enzymology24803199Resistance to beta-lactam antibiotic may influence nanH gene expression in Trueperella pyogenes isolated from bovine endometritis.Zhang DX, Tian K, Han LM, Wang QX, Liu YC, Tian CL, Liu MCMicrob Pathog10.1016/j.micpath.2014.04.0062014Actinomycetaceae/*drug effects/genetics/isolation & purification, Animals, Anti-Bacterial Agents/*pharmacology, Bacterial Proteins/*biosynthesis, Cattle, Cattle Diseases/*microbiology, Endometritis/microbiology/*veterinary, Female, Gene Expression, Microbial Sensitivity Tests, Real-Time Polymerase Chain Reaction, Reverse Transcriptase Polymerase Chain Reaction, *beta-Lactam Resistance, beta-Lactams/*pharmacologyPhylogeny
Phylogeny28504095Characterization of porcine Trueperella pyogenes by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), molecular typing and antimicrobial susceptibility profiling in Sao Paulo State.Moreno LZ, Matajira CEC, da Costa BLP, Ferreira TSP, Silva GFR, Dutra MC, Gomes VTM, Silva APS, Christ APG, Sato MIZ, Moreno AMComp Immunol Microbiol Infect Dis10.1016/j.cimid.2017.03.0052017Actinomycetaceae/*drug effects/*genetics/isolation & purification, Actinomycetales Infections/epidemiology/*microbiology, Amplified Fragment Length Polymorphism Analysis, Animals, Anti-Bacterial Agents/*pharmacology, *Bacterial Typing Techniques, Brazil/epidemiology, Drug Resistance, Multiple, Bacterial, Genotype, Microbial Sensitivity Tests, Molecular Typing/*methods, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods, SwineGenetics
Phylogeny29528808Phenotypic and genotypic characteristics of Trueperella pyogenes isolated from ruminants.Rogovskyy AS, Lawhon S, Kuczmanski K, Gillis DC, Wu J, Hurley H, Rogovska YV, Konganti K, Yang CY, Duncan KJ Vet Diagn Invest10.1177/10406387187624792018Actinomycetales Infections/microbiology/*veterinary, Animals, Arcanobacterium/*genetics/isolation & purification/pathogenicity, Cattle, Cattle Diseases/microbiology, Genotype, Goat Diseases/microbiology, Goats, Phylogeny, RNA, Ribosomal, 16S/*analysis, Sheep, Sheep Diseases/microbiology, Virulence FactorsGenetics
Pathogenicity32146452Chemical composition and antibacterial activity of essential oils against pathogens often related to cattle endometritis.Braga Paiano R, Bonilla J, Moro de Sousa RL, Micke Moreno A, Sampaio Baruselli PJ Infect Dev Ctries10.3855/jidc.120762020Actinomycetaceae/drug effects, Animals, Anti-Infective Agents/pharmacology, Bacterial Infections/drug therapy/*veterinary, Cattle, Cattle Diseases/*drug therapy/microbiology, Endometritis/drug therapy/microbiology/*veterinary, Escherichia coli/drug effects, Female, Fusobacterium necrophorum/drug effects, Gas Chromatography-Mass Spectrometry, Oils, Volatile/*chemistry/*pharmacology, Staphylococcus aureus/drug effects
32606820The Antibacterial Activity and Mechanism of Action of Luteolin Against Trueperella pyogenes.Guo Y, Liu Y, Zhang Z, Chen M, Zhang D, Tian C, Liu M, Jiang GInfect Drug Resist10.2147/IDR.S2533632020

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8977Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20630)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20630
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
22976A. F. Yassin,H. Hupfer,C. Siering,P. Schumann10.1099/ijs.0.020032-0Comparative chemotaxonomic and phylogenetic studies on the genus Arcanobacterium Collins et al. 1982 emend. Lehnen et al. 2006: proposal for Trueperella gen. nov. and emended description of the genus ArcanobacteriumIJSEM 61: 1265-1274 201120622055
40534Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/14893
45920Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 13230)https://www.ccug.se/strain?id=13230
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68379Automatically annotated from API Coryne
68380Automatically annotated from API rID32A
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69895Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID8153.1StrainInfo: A central database for resolving microbial strain identifiers
122084Curators of the CIPCollection of Institut Pasteur (CIP 103129)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103129