Strain identifier

BacDive ID: 2094

Type strain: Yes

Species: Arcobacter butzleri

Strain Designation: D2686

Strain history: CIP <- 1992, LMG, Campylobacter butzleri <- P. Vandamme, Lab. Microbiol. Univ., Gent, Belgium <- J. Kiehlbauch, CDC: strain D2686

NCBI tax ID(s): 28197 (species)

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General

@ref: 3369

BacDive-ID: 2094

DSM-Number: 8739

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, Gram-negative, motile, rod-shaped, human pathogen

description: Arcobacter butzleri D2686 is a microaerophile, mesophilic, Gram-negative human pathogen that was isolated from human diarrheal stool.

NCBI tax id

  • NCBI tax id: 28197
  • Matching level: species

strain history

@refhistory
3369<- ATCC; ATCC 49616 <- CDC, Atlanta; CDC D2686 <- State of California Microbial Diseases Lab.; USA; <- Los Angeles County Public Health Labs; USA;
67770CECT 8221 <-- DSM 8739 <-- ATCC 49616 <-- J. A. Kiehlbauch; CDC, USA; D2686 <-- State of California Dis. Lab., USA <-- Los Angeles County Public Health Labs., USA.
117081CIP <- 1992, LMG, Campylobacter butzleri <- P. Vandamme, Lab. Microbiol. Univ., Gent, Belgium <- J. Kiehlbauch, CDC: strain D2686

doi: 10.13145/bacdive2094.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Epsilonproteobacteria
  • order: Campylobacterales
  • family: Arcobacteraceae
  • genus: Arcobacter
  • species: Arcobacter butzleri
  • full scientific name: Arcobacter butzleri (Kiehlbauch et al. 1991) Vandamme et al. 1992

  • synonyms

    @refsynonym
    20215Aliarcobacter butzleri
    20215Campylobacter butzleri

@ref: 3369

domain: Bacteria

phylum: Proteobacteria

class: Epsilonproteobacteria

order: Campylobacterales

family: Arcobacteraceae

genus: Arcobacter

species: Arcobacter butzleri

full scientific name: Arcobacter butzleri (Kiehlbauch et al. 1991) Vandamme et al. 1992

strain designation: D2686

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotility
23369negative1.0-3.0 µm0.2-0.4 µmrod-shapedyes
117081negativerod-shapedyes

colony morphology

@refincubation periodcolony sizecolony colorcolony shapemedium used
33691-2 days
233693 days2-4 mmwhitishcircularblood agar plates

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
3369COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
23369blood agar platesyes
38127MEDIUM 6 - Columbia agar with 10 % horse bloodyesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml)
117081CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72
117081CIP Medium 6yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=6

culture temp

@refgrowthtypetemperaturerange
3369positivegrowth37mesophilic
23369positivegrowth37-42
38127positivegrowth37mesophilic
50109positivegrowth30-37mesophilic
67770positivegrowth37mesophilic
117081positivegrowth10-30
117081nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
3369microaerophile
50109microaerophile

halophily

@refsaltgrowthtested relationconcentration
23369NaClpositivegrowth1.5 %
23369NaClpositivegrowth3.5 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2336917634D-glucose-hydrolysis
23369606565hippurate-hydrolysis
2336917634D-glucose-oxidation
2336917632nitrate+reduction
6837316199urea-hydrolysis
6837317632nitrate+reduction
68373606565hippurate-hydrolysis
6837317634D-glucose-assimilation
6837325115malate-assimilation
6837717634D-glucose-builds acid from
6837715824D-fructose-builds acid from
6837717306maltose-builds acid from
6837718257ornithine+degradation
6837727897tryptophan-energy source

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitivesensitivity conc.is resistant
233693542cephalothinyesno
23369100147nalidixic acidyesyes
6837348923erythromycinyesyes14 µg
1170810129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)yesnoyes

metabolite production

@refChebi-IDmetaboliteproduction
6837735581indoleno
6837316136hydrogen sulfideno
11708135581indoleno

metabolite tests

  • @ref: 68377
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
3369catalase+1.11.1.6
3369cytochrome-c oxidase+1.9.3.1
23369catalase+1.11.1.6
68377tryptophan deaminase-4.1.99.1
68377beta-galactosidase-3.2.1.23
68377alkaline phosphatase-3.1.3.1
68377lipase-
68377ornithine decarboxylase+4.1.1.17
68377beta-lactamase-3.5.2.6
68373alkaline phosphatase+3.1.3.1
68373L-aspartate arylamidase-3.4.11.21
68373L-arginine arylamidase-
68373pyrrolidonyl arylamidase-3.4.19.3
68373gamma-glutamyltransferase-2.3.2.2
68373esterase+
68373urease-3.5.1.5
117081oxidase+
117081gelatinase-
117081catalase+1.11.1.6
117081urease-3.5.1.5

API CAM

@refURENITESTHIPGGTTTCPYRAArgAAspAPALH2SGLUSUTNALCFZACEPROPMLTCITEROCAT
3369-++------+--++/-++/-+-+-+
3369-++--+---+--+++++-+-+
3369-++--+---+--++++----+
3369-++--+---+--+++++---+
3369-++--+---+--+++/-++---+/-
3369-++------+---+/--+/-+---+
3369-++------+--+++/-++---

API NH

@refPENGLUFRUMALSACODCURELIPPALbeta GALProAGGTIND
3369----++----+-
3369----++----+-

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
117081---------------------------------------------------------------------------------------------------

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentgeographic location
3369human diarrheal stoolUSAUSANorth America
50109Human feces,diarrhoeaUSAUSANorth America
67770Stool of a male patient with diarrheal illnessUSAUSANorth AmericaCA
117081Human, FecesUnited States of AmericaUSANorth America

isolation source categories

Cat1Cat2Cat3
#Host#Human
#Host Body Product#Gastrointestinal tract#Feces (Stool)

taxonmaps

  • @ref: 69479
  • File name: preview.99_158.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_53;96_117;97_124;98_139;99_158&stattab=map
  • Last taxonomy: Aliarcobacter butzleri
  • 16S sequence: JX912503
  • Sequence Identity:
  • Total samples: 16332
  • soil counts: 907
  • aquatic counts: 9551
  • animal counts: 5473
  • plant counts: 401

Safety information

risk assessment

@refpathogenicity humanpathogenicity animalbiosafety levelbiosafety level comment
3369yesyes2Risk group (German classification)
1170812Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Arcobacter butzleri strain ATCC 49616 16S ribosomal RNA gene, partial sequenceJX9125031439ena28197
3369Arcobacter butzleri strain ATCC 49616 16S ribosomal RNA gene, partial sequenceAY6211161440ena28197

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Aliarcobacter butzleri NCTC 12481GCA_900187115completencbi28197
66792Aliarcobacter butzleri RM4018GCA_000014025completencbi367737
66792Aliarcobacter butzleri JCM 31724GCA_014648215contigncbi28197
66792Arcobacter butzleri RM4018367737.6completepatric367737
66792Arcobacter butzleri strain JCM 3172428197.218wgspatric28197
66792Arcobacter butzleri strain NCTC 1248128197.59completepatric28197
66792Arcobacter butzleri strain NCTC1248128197.75plasmidpatric28197
66792Aliarcobacter butzleri NCTC 124812799112925completeimg28197
66792Aliarcobacter butzleri RM4018640753004completeimg367737
66792Aliarcobacter butzleri LMG 10828GCA_024584145contigncbi28197

GC content

  • @ref: 23369
  • GC-content: 27.0-30.0
  • method: Thermal denaturation, fluorometry

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileyes90.042yes
flagellatedyes68.513no
gram-positiveno98.701no
anaerobicno75.841no
aerobicno91.544yes
halophileno87.869no
spore-formingno95.938no
thermophileno95.708yes
glucose-utilno88.012no
glucose-fermentno88.469no

External links

@ref: 3369

culture collection no.: DSM 8739, ATCC 49616, CDC D2686, LMG 10828, WDCM 00065, CCUG 30485, NCTC 12481, RM 4018, JCM 31724, CECT 8221, CIP 103493

straininfo link

  • @ref: 71741
  • straininfo: 917

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Enzymology8675340Infection of cesarean-derived colostrum-deprived 1-day-old piglets with Arcobacter butzleri, Arcobacter cryaerophilus, and Arcobacter skirrowii.Wesley IV, Baetz AL, Larson DJInfect Immun10.1128/iai.64.6.2295-2299.19961996Animals, Animals, Newborn, Campylobacter/genetics/isolation & purification/*pathogenicity, Campylobacter Infections/*microbiology/pathology, Cesarean Section, Colostrum, Disease Models, Animal, Female, Polymorphism, Restriction Fragment Length, Pregnancy, SwinePhylogeny
Phylogeny9453132Multiplex PCR for the identification of Arcobacter and differentiation of Arcobacter butzleri from other arcobacters.Harmon KM, Wesley IVVet Microbiol10.1016/s0378-1135(97)00151-x1997Animals, Bacteroides/classification, Campylobacter/classification, Chickens/microbiology, Gram-Negative Bacteria/*classification/genetics/isolation & purification, Gram-Negative Bacterial Infections/classification/diagnosis/*veterinary, Helicobacter/classification, Polymerase Chain Reaction/*methods, Poultry/*microbiology, *Poultry Diseases, RNA, Ribosomal, 16S/genetics, Serotyping, Turkeys/microbiology, Wolinella/classificationEnzymology
Phylogeny10872684Isolation of Arcobacter spp. from a brackish environment.Maugeri TL, Gugliandolo C, Carbone M, Caccamo D, Fera MTNew Microbiol2000Animals, Arcobacter/*isolation & purification, Bacterial Typing Techniques, Bivalvia/*microbiology, *Food Microbiology, Phenotype, *Water MicrobiologyBiotechnology
Cultivation11722672The recovery of Arcobacter butzleri NCTC 12481 from various temperature treatments.Hilton CL, Mackey BM, Hargreaves AJ, Forsythe SJJ Appl Microbiol10.1046/j.1365-2672.2001.01457.x2001Animals, Arcobacter/*growth & development/isolation & purification, Cell Membrane Permeability, Culture Media, Electric Impedance, Gram-Negative Bacterial Infections/microbiology, Humans, Hydrogen-Ion Concentration, *TemperaturePathogenicity
Pathogenicity15501649Survival and injury of Arcobacter after artificial inoculation into drinking water.Moreno Y, Alonso JL, Botella S, Ferrus MA, Hernandez JRes Microbiol10.1016/j.resmic.2004.05.0112004Arcobacter/*drug effects/genetics/*growth & development, Cell Membrane Permeability/drug effects, Chlorine/*pharmacology, Colony Count, Microbial, DNA, Ribosomal/analysis, Disinfection/methods, Fresh Water/*microbiology, In Situ Hybridization, Fluorescence, Microscopy, Electron, Scanning, Polymerase Chain Reaction, Polymorphism, Restriction Fragment Length, RNA, Ribosomal, 23S/genetics, *Water SupplyPhylogeny
Genetics16499619Genome mapping of Arcobacter butzleri.Stoeva K, Bruce Ward FFEMS Microbiol Lett10.1111/j.1574-6968.2006.00135.x2006Arcobacter/*genetics, Campylobacter jejuni/genetics, Chromosome Mapping, DNA, Bacterial/chemistry/genetics/metabolism, Deoxyribonucleases, Type II Site-Specific, Gene Dosage, Gene Order, *Genome, Bacterial, Glycosylation, Molecular Sequence Data, Polymerase Chain Reaction, RNA, Ribosomal, 16S/genetics, RNA, Ribosomal, 23S/genetics, Restriction Mapping, Sequence Analysis, DNA, Synteny, Wolinella/geneticsTranscriptome
Genetics18159241The complete genome sequence and analysis of the epsilonproteobacterium Arcobacter butzleri.Miller WG, Parker CT, Rubenfield M, Mendz GL, Wosten MM, Ussery DW, Stolz JF, Binnewies TT, Hallin PF, Wang G, Malek JA, Rogosin A, Stanker LH, Mandrell REPLoS One10.1371/journal.pone.00013582007Amino Acids, Sulfur/biosynthesis, Arcobacter/classification/*genetics/metabolism/pathogenicity, Bacterial Proteins/chemistry/genetics, Base Pair Mismatch, Base Sequence, Chemotaxis, DNA Primers, DNA Repair, Electrophoresis, Polyacrylamide Gel, *Genome, Bacterial, Oxidation-Reduction, Phylogeny, Signal Transduction, Sulfur/metabolism, Virulence/geneticsPhylogeny
18378639Induction and resuscitation of viable nonculturable Arcobacter butzleri cells.Fera MT, Maugeri TL, Gugliandolo C, La Camera E, Lentini V, Favaloro A, Bonanno D, Carbone MAppl Environ Microbiol10.1128/AEM.00059-082008Arcobacter/*growth & development/*physiology, Colony Count, Microbial, In Situ Hybridization, Fluorescence, Microbial Viability, Microscopy, Fluorescence, Seawater/*microbiology, Temperature, Time Factors
Biotechnology19297052Growth and survival at chiller temperatures of Arcobacter butzleri.Kjeldgaard J, Jorgensen K, Ingmer HInt J Food Microbiol10.1016/j.ijfoodmicro.2009.02.0172009Abattoirs, Animals, Arcobacter/genetics/*growth & development/isolation & purification, Biofilms/*growth & development, Chickens/*microbiology, *Cold Temperature, *Food Microbiology, Meat/*microbiology, *Microbial ViabilityEnzymology
Phylogeny22170914Occurrence of putative virulence genes in arcobacter species isolated from humans and animals.Douidah L, de Zutter L, Bare J, De Vos P, Vandamme P, Vandenberg O, Van den Abeele AM, Houf KJ Clin Microbiol10.1128/JCM.05872-112011Animals, Arcobacter/*genetics/isolation & purification/*pathogenicity, Campylobacter/genetics, Genes, Bacterial, Gram-Negative Bacterial Infections/*microbiology/*veterinary, Humans, Nucleic Acid Hybridization, Polymerase Chain Reaction, Sequence Analysis, DNA, Virulence Factors/*geneticsPathogenicity
24102050Bioaccumulation experiments in mussels contaminated with the food-borne pathogen Arcobacter butzleri: preliminary data for risk assessment.Ottaviani D, Chierichetti S, Rocchegiani E, Bartolini C, Masini L, Santarelli S, Leoni FBiomed Res Int10.1155/2013/1534192013Animals, Arcobacter/*growth & development/pathogenicity, Bivalvia/*microbiology, Food Analysis, Foodborne Diseases/*microbiology, Risk Assessment, Seawater/microbiology
Metabolism26406497Arcobacter butzleri Induce Colonic, Extra-Intestinal and Systemic Inflammatory Responses in Gnotobiotic IL-10 Deficient Mice in a Strain-Dependent Manner.Golz G, Karadas G, Alutis ME, Fischer A, Kuhl AA, Breithaupt A, Gobel UB, Alter T, Bereswill S, Heimesaat MMPLoS One10.1371/journal.pone.01394022015Adaptive Immunity, Administration, Oral, Animals, Apoptosis, Arcobacter/growth & development/*physiology, Bacterial Translocation, Cell Proliferation, Colon/*microbiology/*pathology, Colony Count, Microbial, Cytokines/metabolism, Feces/microbiology, *Germ-Free Life, Gram-Negative Bacterial Infections/microbiology, Immunity, Innate, Inflammation/microbiology/*pathology, Interleukin-10/*deficiency/metabolism, Mice, Inbred C57BL, Nitric Oxide/metabolismPathogenicity
26483849Survey of small intestinal and systemic immune responses following murine Arcobacter butzleri infection.Heimesaat MM, Karadas G, Alutis M, Fischer A, Kuhl AA, Breithaupt A, Gobel UB, Alter T, Bereswill S, Golz GGut Pathog10.1186/s13099-015-0075-z2015
26716022Toll-Like Receptor-4 Dependent Small Intestinal Immune Responses Following Murine Arcobacter Butzleri Infection.Heimesaat MM, Karadas G, Fischer A, Gobel UB, Alter T, Bereswill S, Golz GEur J Microbiol Immunol (Bp)10.1556/1886.2015.000422015
Pathogenicity27141315Intestinal Expression of Genes Encoding Inflammatory Mediators and Gelatinases During Arcobacter Butzleri Infection of Gnotobiotic Il-10 Deficient Mice.Heimesaat MM, Alter T, Bereswill S, Golz GEur J Microbiol Immunol (Bp)10.1556/1886.2016.000052016
27141316Toll-Like Receptor-4 Dependent Intestinal Gene Expression During Arcobacter Butzleri Infection of Gnotobiotic Il-10 Deficient Mice.Golz G, Alter T, Bereswill S, Heimesaat MMEur J Microbiol Immunol (Bp)10.1556/1886.2016.000062016
Metabolism27438014The Immunopathogenic Potential of Arcobacter butzleri - Lessons from a Meta-Analysis of Murine Infection Studies.Golz G, Alter T, Bereswill S, Heimesaat MMPLoS One10.1371/journal.pone.01596852016Animals, Arcobacter/*immunology/pathogenicity, Campylobacter jejuni/immunology/pathogenicity, Chemokine CCL2/metabolism, Colon/immunology/microbiology/pathology, Disease Models, Animal, Escherichia coli/immunology/pathogenicity, Gram-Negative Bacterial Infections/*immunology/microbiology/pathology, Humans, Inflammation/*immunology/metabolism/microbiology, Interferon-gamma/metabolism, Interleukin-10/metabolism, Intestines/*immunology/microbiology/pathology, Mice, Tumor Necrosis Factor-alpha/metabolismPathogenicity
Genetics28671965Characterization of the emerging zoonotic pathogen Arcobacter thereius by whole genome sequencing and comparative genomics.Rovetto F, Carlier A, Van den Abeele AM, Illeghems K, Van Nieuwerburgh F, Cocolin L, Houf KPLoS One10.1371/journal.pone.01804932017Animals, Anti-Bacterial Agents/pharmacology, Arcobacter/classification/drug effects/*genetics, Ducks/microbiology, *Genome, Bacterial, High-Throughput Nucleotide Sequencing/*methods, Humans, Microbial Sensitivity Tests, Phylogeny, Swine/microbiologyPhylogeny
Phylogeny30394871Arcobacter lacus sp. nov. and Arcobacter caeni sp. nov., two novel species isolated from reclaimed water.Perez-Cataluna A, Salas-Masso N, Figueras MJInt J Syst Evol Microbiol10.1099/ijsem.0.0031012019Arcobacter/*classification/isolation & purification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Genes, Bacterial, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Spain, Waste Water/*microbiology, *Water Microbiology, Water PurificationEnzymology

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
3369Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 8739)https://www.dsmz.de/collection/catalogue/details/culture/DSM-8739
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
23369P. Vandamme, M. Vancanneyt, B. Pot, L. Mels, B. Hoste, D. Dewettinck, L. Vlaes, C. Van Den Borre, R. Higgins, J. Hommez, K. Kersters, J.-P. Butzler, H. Goossens10.1099/00207713-42-3-344Polyphasic Taxonomic Study of the Emended Genus Arcobacter with Arcobacter butzleri comb. nov. and Arcobacter skirrowii sp. nov., an Aerotolerant Bacterium Isolated from Veterinary SpecimensIJSEM 42: 344-356 19921503968
38127Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/15296
50109Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 30485)https://www.ccug.se/strain?id=30485
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68373Automatically annotated from API CAM
68377Automatically annotated from API NH
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
71741Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID917.1StrainInfo: A central database for resolving microbial strain identifiers
117081Curators of the CIPCollection of Institut Pasteur (CIP 103493)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103493