Strain identifier

BacDive ID: 209

Type strain: Yes

Species: Mobiluncus holmesii

Strain Designation: BV376-6, Spiegel BV376-6

Strain history: <- LMG <- CCUG <- NCTC <- C. A. Spiegel, Medical College of Wisconsin, USA; BV376-6

NCBI tax ID(s): 887899 (strain), 144178 (species)

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General

@ref: 15870

BacDive-ID: 209

DSM-Number: 21655

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic, Gram-variable, motile, rod-shaped

description: Mobiluncus holmesii BV376-6 is an anaerobe, mesophilic, Gram-variable bacterium that was isolated from vaginae of women with bacterial vaginosis.

NCBI tax id

NCBI tax idMatching level
144178species
887899strain

strain history

  • @ref: 15870
  • history: <- LMG <- CCUG <- NCTC <- C. A. Spiegel, Medical College of Wisconsin, USA; BV376-6

doi: 10.13145/bacdive209.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Actinomycetales
  • family: Actinomycetaceae
  • genus: Mobiluncus
  • species: Mobiluncus holmesii
  • full scientific name: Mobiluncus holmesii (Spiegel and Roberts 1984) Nouioui et al. 2018

  • synonyms

    @refsynonym
    20215Falcivibrio vaginalis
    20215Mobiluncus curtisii subsp. holmesii

@ref: 15870

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Actinomycetaceae

genus: Mobiluncus

species: Mobiluncus holmesii

full scientific name: Mobiluncus holmesii (Spiegel and Roberts 1984) Nouioui et al. 2018

strain designation: BV376-6, Spiegel BV376-6

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityflagellum arrangementconfidence
22975variable1.7 µm<0.5 µmrod-shapedyeslophotrichous
69480positive100

colony morphology

@reftype of hemolysisincubation periodcolony sizecolony color
15870gamma3-7 days
229755 days2.0-3.0 mmcolorless

Culture and growth conditions

culture medium

  • @ref: 15870
  • name: BIFIDOBACTERIUM MEDIUM (DSMZ Medium 58)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/58
  • composition: Name: BIFIDOBACTERIUM MEDIUM (DSMZ Medium 58) Composition: Glucose 10.0 g/l Casein peptone 10.0 g/l Bacto Soytone 5.0 g/l Meat extract 5.0 g/l Yeast extract 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l MnSO4 x H2O 0.05 g/l KH2PO4 0.04 g/l K2HPO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Tween 80 Resazurin Distilled water

culture temp

@refgrowthtypetemperaturerange
15870positivegrowth37mesophilic
22975positivegrowth35.0mesophilic
22975positivegrowth42.0thermophilic
22975nogrowth45.0thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
15870anaerobe
22975anaerobe
46686anaerobe
69480anaerobe97.057

spore formation

@refspore formationconfidence
22975no
69481no100
69480no100

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2297515963ribitol-builds acid from
2297527613amygdalin-builds acid from
2297529016arginine-builds acid from
2297517057cellobiose-builds acid from
2297517113erythritol-builds acid from
229754853esculin-builds acid from
2297515443inulin-builds acid from
2297529864mannitol-builds acid from
229756731melezitose-builds acid from
2297517814salicin-builds acid from
2297530911sorbitol-builds acid from
2297522599arabinose-fermentation
2297528757fructose-fermentation
2297528260galactose-fermentation
2297517234glucose-fermentation
2297528087glycogen-fermentation
2297517268myo-inositol-fermentation
2297517716lactose-fermentation
2297537684mannose-fermentation
2297528053melibiose-fermentation
2297516634raffinose-fermentation
2297526546rhamnose-fermentation
2297533942ribose-fermentation
2297528017starch-fermentation
2297517992sucrose-fermentation
2297527082trehalose-fermentation
2297518222xylose-fermentation
229754853esculin-hydrolysis
2297517306maltose+fermentation
2297528017starch+hydrolysis
2297517632nitrate+reduction
6838016199urea-hydrolysis
6838029016arginine-hydrolysis
6838016024D-mannose-fermentation
6838029985L-glutamate-degradation
6838017632nitrate-reduction
6838027897tryptophan-energy source

metabolite production

@refChebi-IDmetaboliteproduction
2297535581indoleno
2297533403elemental sulfurno
6838035581indoleno

metabolite tests

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
22975catalase-1.11.1.6
22975cytochrome oxidase-1.9.3.1
22975esterase lipase (C 8)+
22975lecithinase-
22975lipase-
68380serine arylamidase-
68380glutamyl-glutamate arylamidase-
68380histidine arylamidase+
68380glycin arylamidase+
68380alanine arylamidase-3.4.11.2
68380tyrosine arylamidase-
68380pyrrolidonyl arylamidase-3.4.19.3
68380leucine arylamidase-3.4.11.1
68380phenylalanine arylamidase-
68380leucyl glycin arylamidase+3.4.11.1
68380proline-arylamidase+3.4.11.5
68380L-arginine arylamidase+
68380alkaline phosphatase-3.1.3.1
68380tryptophan deaminase-4.1.99.1
68380alpha-fucosidase-3.2.1.51
68380glutamate decarboxylase-4.1.1.15
68380N-acetyl-beta-glucosaminidase-3.2.1.52
68380beta-glucuronidase-3.2.1.31
68380alpha-arabinosidase-3.2.1.55
68380beta-glucosidase-3.2.1.21
68380alpha-glucosidase+3.2.1.20
68380beta-Galactosidase 6-phosphate-
68380beta-galactosidase+3.2.1.23
68380alpha-galactosidase+3.2.1.22
68380arginine dihydrolase-3.5.3.6
68380urease-3.5.1.5

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
15870--++-+-----+/------+++-----++--

Isolation, sampling and environmental information

isolation

@refsample type
22975vaginae of women with bacterial vaginosis
46686Human vagina
15870human vagina

isolation source categories

Cat1Cat2Cat3
#Infection#Disease
#Host#Human#Female
#Host Body-Site#Urogenital tract#Vagina

taxonmaps

  • @ref: 69479
  • File name: preview.99_6044.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_16139;96_2409;97_2932;98_4483;99_6044&stattab=map
  • Last taxonomy: Mobiluncus
  • 16S sequence: AJ427624
  • Sequence Identity:
  • Total samples: 34430
  • soil counts: 819
  • aquatic counts: 629
  • animal counts: 32804
  • plant counts: 178

Safety information

risk assessment

  • @ref: 15870
  • biosafety level: 2
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Mobiluncus curtisii subsp. holmesii partial 16S rRNA gene, strain CCUG 17762TAJ3184091500ena887899
20218M.curtisii 16S rRNA gene (ssp. holmesii)X82604369ena2051
20218M.curtisii 16S rRNA gene (ssp. holmesii)X86006331ena2051
15870Mobiluncus curtisii subsp. holmesii partial 16S rRNA gene, type strain CCUG 17762TAJ4276241477ena887899

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Mobiluncus holmesii ATCC 35242GCA_000185445scaffoldncbi887899
66792Mobiluncus holmesii NCTC11657GCA_900453235contigncbi144178
66792Mobiluncus curtisii subsp. holmesii ATCC 35242887899.3wgspatric887899
66792Mobiluncus curtisii subsp. holmesii strain NCTC11657144178.3wgspatric144178
66792Mobiluncus curtisii holmesii NCTC 116572840375602draftimg2051
66792Mobiluncus curtisii holmesii ATCC 35242649989974draftimg887899

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileyes68.014yes
flagellatedno77.307no
gram-positiveyes66.111no
anaerobicyes93.909yes
aerobicno97.121yes
halophileno92.536no
spore-formingno93.999yes
glucose-utilyes65.947no
thermophileno67.184no
glucose-fermentyes81.701yes

External links

@ref: 15870

culture collection no.: DSM 21655, ATCC 35242, CCUG 17762, LMG 7786, NCTC 11657

straininfo link

  • @ref: 69892
  • straininfo: 3578

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
15870Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 21655)https://www.dsmz.de/collection/catalogue/details/culture/DSM-21655
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
22975Carol A. Spiegel,Marilyn Roberts10.1099/00207713-34-2-177Mobiluncus gen. nov., Mobiluncus curtisii subsp. curtisii sp. nov., Mobiluncus curtisii subsp. holmesii subsp. nov., and Mobiluncus mulieris sp. nov., Curved Rods from the human VaginaIJSEM 34: 177-184 1984
46686Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 17762)https://www.ccug.se/strain?id=17762
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68380Automatically annotated from API rID32A
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
69892Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID3578.1StrainInfo: A central database for resolving microbial strain identifiers