Strain identifier
BacDive ID: 207
Type strain:
Species: Mobiluncus holmesii
Strain Designation: V125
Strain history: <- R. Hammann <- R. Durieux, V125
NCBI tax ID(s): 144178 (species)
General
@ref: 1211
BacDive-ID: 207
DSM-Number: 2711
keywords: genome sequence, Bacteria, anaerobe, mesophilic, Gram-positive
description: Mobiluncus holmesii V125 is an anaerobe, mesophilic, Gram-positive bacterium that was isolated from human vagina.
NCBI tax id
- NCBI tax id: 144178
- Matching level: species
strain history
- @ref: 1211
- history: <- R. Hammann <- R. Durieux, V125
doi: 10.13145/bacdive207.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Actinomycetales
- family: Actinomycetaceae
- genus: Mobiluncus
- species: Mobiluncus holmesii
- full scientific name: Mobiluncus holmesii (Spiegel and Roberts 1984) Nouioui et al. 2018
synonyms
@ref synonym 20215 Falcivibrio vaginalis 20215 Mobiluncus curtisii subsp. holmesii
@ref: 1211
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Actinomycetales
family: Actinomycetaceae
genus: Mobiluncus
species: Mobiluncus holmesii
full scientific name: Mobiluncus holmesii (Spiegel and Roberts 1984) Nouioui et al. 2018
strain designation: V125
type strain: no
Morphology
cell morphology
- @ref: 69480
- gram stain: positive
- confidence: 100
colony morphology
@ref | incubation period |
---|---|
1211 | 1-2 days |
48016 | 3 days |
Culture and growth conditions
culture medium
- @ref: 1211
- name: FALCIVIBRIO MEDIUM (DSMZ Medium 301)
- growth: yes
- link: https://mediadive.dsmz.de/medium/301
- composition: Name: FALCIVIBRIO MEDIUM (DSMZ Medium 301) Composition: L-Cysteine 0.3 g/l Resazurin 0.001 g/l Wilkins-Chalgren-Anaerobe-Broth Sterile serum
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
1211 | positive | growth | 37 | mesophilic |
48016 | positive | growth | 37 | mesophilic |
Physiology and metabolism
oxygen tolerance
@ref | oxygen tolerance | confidence |
---|---|---|
1211 | anaerobe | |
48016 | anaerobe | |
69480 | anaerobe | 99.209 |
spore formation
@ref | spore formation | confidence |
---|---|---|
69481 | no | 100 |
69480 | no | 100 |
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
68373 | 16199 | urea | - | hydrolysis |
68373 | 17632 | nitrate | + | reduction |
68373 | 606565 | hippurate | - | hydrolysis |
68373 | 78019 | triphenyltetrazolium chloride | + | reduction |
68373 | 17634 | D-glucose | - | assimilation |
68373 | 30031 | succinate | - | assimilation |
68373 | 30089 | acetate | - | assimilation |
68373 | 17272 | propionate | - | assimilation |
68373 | 25115 | malate | - | assimilation |
68373 | 16947 | citrate | - | assimilation |
68380 | 16199 | urea | - | hydrolysis |
68380 | 29016 | arginine | + | hydrolysis |
68380 | 16024 | D-mannose | - | fermentation |
68380 | 16634 | raffinose | + | fermentation |
68380 | 29985 | L-glutamate | - | degradation |
68380 | 17632 | nitrate | - | reduction |
68380 | 27897 | tryptophan | - | energy source |
antibiotic resistance
@ref | ChEBI | metabolite | is antibiotic | is sensitive | sensitivity conc. |
---|---|---|---|---|---|
68373 | 48923 | erythromycin | yes | yes | 14 µg |
68373 | 474053 | cefazolin | yes | yes | 224 µg |
68373 | 100147 | nalidixic acid | yes | yes | 84 µg |
metabolite production
@ref | Chebi-ID | metabolite | production |
---|---|---|---|
68373 | 16136 | hydrogen sulfide | no |
68380 | 35581 | indole | no |
metabolite tests
- @ref: 68380
- Chebi-ID: 35581
- metabolite: indole
- indole test: -
enzymes
@ref | value | activity | ec |
---|---|---|---|
68382 | alpha-fucosidase | - | 3.2.1.51 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68382 | beta-glucosidase | - | 3.2.1.21 |
68382 | alpha-glucosidase | + | 3.2.1.20 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | beta-galactosidase | + | 3.2.1.23 |
68382 | alpha-galactosidase | + | 3.2.1.22 |
68382 | naphthol-AS-BI-phosphohydrolase | - | |
68382 | acid phosphatase | - | 3.1.3.2 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | trypsin | - | 3.4.21.4 |
68382 | cystine arylamidase | - | 3.4.11.3 |
68382 | valine arylamidase | - | |
68382 | leucine arylamidase | + | 3.4.11.1 |
68382 | lipase (C 14) | - | |
68382 | esterase lipase (C 8) | + | |
68382 | esterase (C 4) | + | |
68382 | alkaline phosphatase | - | 3.1.3.1 |
68380 | serine arylamidase | - | |
68380 | glutamyl-glutamate arylamidase | - | |
68380 | histidine arylamidase | + | |
68380 | glycin arylamidase | + | |
68380 | alanine arylamidase | + | 3.4.11.2 |
68380 | tyrosine arylamidase | + | |
68380 | pyrrolidonyl arylamidase | - | 3.4.19.3 |
68380 | leucine arylamidase | + | 3.4.11.1 |
68380 | phenylalanine arylamidase | + | |
68380 | leucyl glycin arylamidase | + | 3.4.11.1 |
68380 | proline-arylamidase | + | 3.4.11.5 |
68380 | L-arginine arylamidase | + | |
68380 | alkaline phosphatase | - | 3.1.3.1 |
68380 | tryptophan deaminase | - | 4.1.99.1 |
68380 | alpha-fucosidase | - | 3.2.1.51 |
68380 | glutamate decarboxylase | - | 4.1.1.15 |
68380 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68380 | beta-glucuronidase | - | 3.2.1.31 |
68380 | alpha-arabinosidase | - | 3.2.1.55 |
68380 | beta-glucosidase | - | 3.2.1.21 |
68380 | alpha-glucosidase | + | 3.2.1.20 |
68380 | beta-Galactosidase 6-phosphate | - | |
68380 | beta-galactosidase | + | 3.2.1.23 |
68380 | alpha-galactosidase | + | 3.2.1.22 |
68380 | arginine dihydrolase | + | 3.5.3.6 |
68380 | urease | - | 3.5.1.5 |
68373 | catalase | - | 1.11.1.6 |
68373 | alkaline phosphatase | + | 3.1.3.1 |
68373 | L-aspartate arylamidase | + | 3.4.11.21 |
68373 | L-arginine arylamidase | + | |
68373 | pyrrolidonyl arylamidase | - | 3.4.19.3 |
68373 | gamma-glutamyltransferase | - | 2.3.2.2 |
68373 | esterase | + | |
68373 | urease | - | 3.5.1.5 |
API zym
@ref | Control | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
48016 | - | - | + | + | - | + | - | - | - | - | - | - | + | + | - | + | - | - | - | - |
API CAM
@ref | URE | NIT | EST | HIP | GGT | TTC | PYRA | ArgA | AspA | PAL | H2S | GLU | SUT | NAL | CFZ | ACE | PROP | MLT | CIT | ERO | CAT |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
48016 | - | + | + | - | - | + | - | + | + | + | - | - | - | - | - | - | - | - | - | - | - |
API rID32A
@ref | URE | ADH Arg | alpha GAL | beta GAL | beta GP | alpha GLU | beta GLU | alpha ARA | beta GUR | beta NAG | MNE | RAF | GDC | alpha FUC | NIT | IND | PAL | ArgA | ProA | LGA | PheA | LeuA | PyrA | TyrA | AlaA | GlyA | HisA | GGA | SerA |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
48016 | - | + | + | + | - | + | - | - | - | - | - | + | - | - | - | - | - | + | + | + | + | + | - | + | + | + | + | - | - |
Isolation, sampling and environmental information
isolation
@ref | sample type | country | origin.country | continent | geographic location |
---|---|---|---|---|---|
1211 | human vagina | France | FRA | Europe | |
48016 | Human vagina,vaginitis | France | FRA | Europe | Villeneuve-St.Georges |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Host Body-Site | #Urogenital tract | #Vagina |
#Host | #Human | #Female |
Safety information
risk assessment
- @ref: 1211
- biosafety level: 2
- biosafety level comment: Risk group (German classification)
Sequence information
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Mobiluncus curtisii ATCC 43063 | GCA_000196535 | complete | ncbi | 548479 |
66792 | Mobiluncus curtisii ATCC 43063 | 548479.6 | complete | patric | 548479 |
66792 | Mobiluncus curtisii strain FDAARGOS_1128 | 2051.56 | complete | patric | 2051 |
66792 | Mobiluncus curtisii ATCC 43063 | 648028043 | complete | img | 548479 |
GC content
- @ref: 1211
- GC-content: 53.9
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
spore-forming | no | 100 | no |
motile | yes | 69.423 | no |
flagellated | no | 77.718 | no |
gram-positive | yes | 70.636 | no |
anaerobic | yes | 95.406 | no |
aerobic | no | 97.361 | yes |
halophile | no | 93.079 | no |
spore-forming | no | 93.806 | no |
thermophile | no | 61.531 | no |
glucose-util | yes | 67.901 | yes |
glucose-ferment | yes | 82.525 | no |
External links
@ref: 1211
culture collection no.: DSM 2711, ATCC 43063, CCUG 24716
straininfo link
- @ref: 69890
- straininfo: 2810
Reference
@id | authors | catalogue | doi/url | title |
---|---|---|---|---|
1211 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 2711) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-2711 | |
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
48016 | Curators of the CCUG | Culture Collection University of Gothenburg (CCUG) (CCUG 24716) | https://www.ccug.se/strain?id=24716 | |
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |
68373 | Automatically annotated from API CAM | |||
68380 | Automatically annotated from API rID32A | |||
68382 | Automatically annotated from API zym | |||
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |
69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |
69890 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID2810.1 | StrainInfo: A central database for resolving microbial strain identifiers |