Strain identifier

BacDive ID: 2067

Type strain: Yes

Species: Paucimonas lemoignei

Strain history: CIP <- 1993, ATCC <- N. J. Palleroni

NCBI tax ID(s): 29443 (species)

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General

@ref: 3152

BacDive-ID: 2067

DSM-Number: 7445

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, thermophilic, Gram-negative, motile, rod-shaped

description: Paucimonas lemoignei DSM 7445 is an obligate aerobe, thermophilic, Gram-negative bacterium that was isolated from soil, poly-ß-hydroxybutyrate enriched.

NCBI tax id

  • NCBI tax id: 29443
  • Matching level: species

strain history

@refhistory
3152<- ATCC <- N.J. Palleroni
120578CIP <- 1993, ATCC <- N. J. Palleroni

doi: 10.13145/bacdive2067.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Betaproteobacteria
  • order: Burkholderiales
  • family: Burkholderiaceae
  • genus: Paucimonas
  • species: Paucimonas lemoignei
  • full scientific name: Paucimonas lemoignei (Delafield et al. 1965) Jendrossek 2001

  • synonyms

    • @ref: 20215
    • synonym: Pseudomonas lemoignei

@ref: 3152

domain: Bacteria

phylum: Proteobacteria

class: Betaproteobacteria

order: Burkholderiales

family: Burkholderiaceae

genus: Paucimonas

species: Paucimonas lemoignei

full scientific name: Paucimonas lemoignei (Delafield et al. 1965) Jendrossek 2001

type strain: yes

Morphology

cell morphology

  • @ref: 120578
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: yes

colony morphology

  • @ref: 44488
  • incubation period: 2 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
3152R2A MEDIUM (DSMZ Medium 830)yeshttps://mediadive.dsmz.de/medium/830Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water
3152HETEROTROPHIC MEDIUM H3P (DSMZ Medium 428)yeshttps://mediadive.dsmz.de/medium/428Name: HETEROTROPHIC MEDIUM H3P (DSMZ Medium 428) Composition: Agar 19.802 g/l Na2HPO4 x 2 H2O 2.87129 g/l KH2PO4 2.27723 g/l D-Glucose 1.9802 g/l Na-lactate 0.990099 g/l Na-pyruvate 0.990099 g/l D-Mannitol 0.990099 g/l DL-Malate 0.990098 g/l Na-acetate 0.990098 g/l Yeast extract 0.990098 g/l NH4Cl 0.990098 g/l Disodium succinate 0.990098 g/l MgSO4 x 7 H2O 0.495049 g/l Ferric ammonium citrate 0.049505 g/l CaCl2 x 2 H2O 0.00990098 g/l NaVO3 x H2O 0.00495049 g/l Thiamine-HCl x 2 H2O 0.00247525 g/l Nicotinic acid 0.00247525 g/l Pyridoxine hydrochloride 0.00247525 g/l Calcium pantothenate 0.00247525 g/l H3BO3 0.00148515 g/l CoCl2 x 6 H2O 0.000990099 g/l Riboflavin 0.00049505 g/l ZnSO4 x 7 H2O 0.00049505 g/l MnCl2 x 4 H2O 0.000148515 g/l Na2MoO4 x 2 H2O 0.000148515 g/l NiCl2 x 6 H2O 9.90099e-05 g/l CuCl2 x 2 H2O 4.95049e-05 g/l Vitamin B12 4.95049e-05 g/l Folic acid 9.90099e-06 g/l Biotin 4.9505e-06 g/l Distilled water
40788MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
3152TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium535.pdf
3152MINERAL MEDIUM (BRUNNER) (DSMZ Medium 457)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium457.pdf
3152CASO AGAR (MERCK 105458) (DSMZ Medium 220)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium220.pdf
120578CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3

culture temp

@refgrowthtypetemperaturerange
3152positivegrowth40thermophilic
3152positivegrowth28mesophilic
40788positivegrowth30mesophilic
44488positivegrowth30mesophilic
120578positivegrowth41thermophilic
120578nogrowth5psychrophilic
120578nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 120578
  • oxygen tolerance: obligate aerobe

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
12057816947citrate-carbon source
1205784853esculin-hydrolysis
12057817632nitrate-reduction
12057816301nitrite-reduction
12057817632nitrate-respiration

enzymes

@refvalueactivityec
120578oxidase+
120578beta-galactosidase+3.2.1.23
120578alcohol dehydrogenase-1.1.1.1
120578gelatinase-
120578amylase-
120578caseinase-3.4.21.50
120578tween esterase-
120578lecithinase-
120578lysine decarboxylase-4.1.1.18
120578ornithine decarboxylase-4.1.1.17

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentgeographic location
3152soil, poly-ß-hydroxybutyrate enrichedUSAUSANorth America
44488Soil,poly-ß-hydroxybutyrate-enrichedUSAUSANorth AmericaBerkeley
120578Environment, Poly-beta-hydroxybutyrate-enriched soil

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
31521Risk group (German classification)
1205781Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Paucimonas lemoignei gene for 16S rRNA, strain: ATCC 17989AB0213751513ena29443
20218P.lemoignei 16S ribosomal RNA (strain LMG 2207T)X925551488ena29443

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Paucimonas lemoignei DSM 7445GCA_004342585scaffoldncbi29443
66792Paucimonas lemoignei NCTC10937GCA_900475325completencbi29443
66792Paucimonas lemoignei strain DSM 744529443.5wgspatric29443
66792Paucimonas lemoignei strain NCTC1093729443.3completepatric29443
66792Paucimonas lemoignei DSM 74452784746777draftimg29443
66792Paucimonas lemoignei NCTC 109372808606625completeimg29443

GC content

  • @ref: 3152
  • GC-content: 58.0
  • method: Buoyant density centrifugation (BD)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
flagellatedyes75.626no
gram-positiveno98.065no
anaerobicno98.305no
aerobicyes93.241no
halophileno91.551no
spore-formingno95.751no
thermophileno99.843no
glucose-utilyes94.547no
motileyes91.139no
glucose-fermentno90.974no

External links

@ref: 3152

culture collection no.: DSM 7445, ATCC 17989, CCUG 2114, NCTC 10937, LMG 2207, CIP 103794, KCTC 32002

straininfo link

  • @ref: 71714
  • straininfo: 4116

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny8782688Isolation and identification of poly(3-hydroxyvalerate)-degrading strains of Pseudomonas lemoignei.Mergaert J, Schirmer A, Hauben L, Mau M, Hoste B, Kersters K, Jendrossek D, Swings JInt J Syst Bacteriol10.1099/00207713-46-3-7691996Bacterial Proteins/analysis, Base Composition, Base Sequence, DNA, Bacterial, Electrophoresis, Polyacrylamide Gel, Fatty Acids/analysis, Molecular Sequence Data, Nucleic Acid Hybridization, Plasmids, Polyesters/*metabolism, Pseudomonas/*classification/genetics/isolation & purification/metabolism, RNA, Bacterial, RNA, Ribosomal, 16S, Soil Microbiology, Valerates/*metabolismEnzymology
Phylogeny28629495Noviherbaspirillum denitrificans sp. nov., a denitrifying bacterium isolated from rice paddy soil and Noviherbaspirillum autotrophicum sp. nov., a denitrifying, facultatively autotrophic bacterium isolated from rice paddy soil and proposal to reclassify Herbaspirillum massiliense as Noviherbaspirillum massiliense comb. nov.Ishii S, Ashida N, Ohno H, Segawa T, Yabe S, Otsuka S, Yokota A, Senoo KInt J Syst Evol Microbiol10.1099/ijsem.0.0018752017Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Denitrification, Fatty Acids/chemistry, Herbaspirillum/*classification/genetics/isolation & purification, *Oryza, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sequence Analysis, DNA, *Soil MicrobiologyTranscriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
3152Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 7445)https://www.dsmz.de/collection/catalogue/details/culture/DSM-7445
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
40788Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/15630
44488Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 2114)https://www.ccug.se/strain?id=2114
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
71714Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID4116.1StrainInfo: A central database for resolving microbial strain identifiers
120578Curators of the CIPCollection of Institut Pasteur (CIP 103794)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103794