Strain identifier

BacDive ID: 206

Type strain: Yes

Species: Mobiluncus mulieris

Strain Designation: SV 175, SV 17J

Strain history: <- LMG <- CCUG <- NCTC <- C. A. Spiegel, Medical College of Wisconsin, USA

NCBI tax ID(s): 585199 (strain), 2052 (species)

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General

@ref: 17992

BacDive-ID: 206

DSM-Number: 25311

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic, Gram-negative, motile, rod-shaped

description: Mobiluncus mulieris SV 175 is an anaerobe, mesophilic, Gram-negative bacterium that was isolated from vaginae of women with bacterial vaginosis.

NCBI tax id

NCBI tax idMatching level
585199strain
2052species

strain history

  • @ref: 17992
  • history: <- LMG <- CCUG <- NCTC <- C. A. Spiegel, Medical College of Wisconsin, USA

doi: 10.13145/bacdive206.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Actinomycetales
  • family: Actinomycetaceae
  • genus: Mobiluncus
  • species: Mobiluncus mulieris
  • full scientific name: Mobiluncus mulieris Spiegel and Roberts 1984

  • synonyms

    • @ref: 20215
    • synonym: Falcivibrio grandis

@ref: 17992

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Actinomycetaceae

genus: Mobiluncus

species: Mobiluncus mulieris

full scientific name: Mobiluncus mulieris Spiegel and Roberts 1984 emend. Hoyles et al. 2004 emend. Nouioui et al. 2018

strain designation: SV 175, SV 17J

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityflagellum arrangementconfidence
22975negative2.9 µm<0.5 µmrod-shapedyeslophotrichous
69480positive99.549

colony morphology

@reftype of hemolysisincubation periodcolony sizecolony color
17992gamma3-7 days
229755 days2.0-3.0 mmcolorless

Culture and growth conditions

culture medium

  • @ref: 17992
  • name: PYG MEDIUM (MODIFIED) (DSMZ Medium 104)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/104
  • composition: Name: PYG MEDIUM (modified) (DSMZ Medium 104) Composition: Yeast extract 10.0 g/l Peptone 5.0 g/l Trypticase peptone 5.0 g/l Beef extract 5.0 g/l Glucose 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l K2HPO4 0.04 g/l KH2PO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Hemin 0.005 g/l Ethanol 0.0038 g/l Resazurin 0.001 g/l Tween 80 Vitamin K1 NaOH Distilled water

culture temp

@refgrowthtypetemperaturerange
17992positivegrowth37mesophilic
22975positivegrowth35.0mesophilic
22975positivegrowth42.0thermophilic
22975nogrowth45.0thermophilic
47098positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
17992anaerobe
22975anaerobe
47098anaerobe
69480anaerobe98.869

spore formation

@refspore formationconfidence
22975no
69481no100
69480no100

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2297515963ribitol-builds acid from
2297527613amygdalin-builds acid from
2297529016arginine-builds acid from
2297517057cellobiose-builds acid from
2297517113erythritol-builds acid from
229754853esculin-builds acid from
2297515443inulin-builds acid from
2297529864mannitol-builds acid from
229756731melezitose-builds acid from
2297517814salicin-builds acid from
2297530911sorbitol-builds acid from
2297528053melibiose-fermentation
2297516634raffinose-fermentation
2297533942ribose-fermentation
2297529016arginine-growth
229754853esculin-hydrolysis
22975606565hippurate-hydrolysis
2297522599arabinose+fermentation
2297528757fructose+fermentation
2297528260galactose+fermentation
2297517234glucose+fermentation
2297528087glycogen+fermentation
2297517268myo-inositol+fermentation
2297517716lactose+fermentation
2297517306maltose+fermentation
2297537684mannose+fermentation
2297526546rhamnose+fermentation
2297528017starch+fermentation
2297517992sucrose+fermentation
2297527082trehalose+fermentation
2297518222xylose+fermentation
2297528017starch+hydrolysis
6838016199urea-hydrolysis
6838029016arginine-hydrolysis
6838016024D-mannose-fermentation
6838016634raffinose-fermentation
6838029985L-glutamate-degradation
6838017632nitrate-reduction
6838027897tryptophan-energy source

metabolite production

@refChebi-IDmetaboliteproduction
2297535581indoleno
2297533403elemental sulfurno
6838035581indoleno

metabolite tests

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
22975alpha-glucosidase+3.2.1.20
22975catalase-1.11.1.6
22975cytochrome oxidase-1.9.3.1
22975esterase lipase (C 8)+
22975gelatinase-
22975lecithinase-
22975leucine arylamidase+3.4.11.1
22975lipase-
22975phosphoamidase+3.9.1.1
68380serine arylamidase-
68380glutamyl-glutamate arylamidase-
68380histidine arylamidase-
68380alanine arylamidase+3.4.11.2
68380tyrosine arylamidase+
68380pyrrolidonyl arylamidase-3.4.19.3
68380leucine arylamidase+3.4.11.1
68380phenylalanine arylamidase+
68380proline-arylamidase+3.4.11.5
68380L-arginine arylamidase+
68380alkaline phosphatase-3.1.3.1
68380tryptophan deaminase-4.1.99.1
68380alpha-fucosidase-3.2.1.51
68380glutamate decarboxylase-4.1.1.15
68380N-acetyl-beta-glucosaminidase-3.2.1.52
68380beta-glucuronidase-3.2.1.31
68380alpha-arabinosidase-3.2.1.55
68380beta-glucosidase-3.2.1.21
68380alpha-glucosidase+3.2.1.20
68380beta-Galactosidase 6-phosphate-
68380beta-galactosidase-3.2.1.23
68380alpha-galactosidase-3.2.1.22
68380arginine dihydrolase-3.5.3.6
68380urease-3.5.1.5

fatty acid profile

  • fatty acids

    @reffatty acidpercentageECL
    47098C14:03.714
    47098C16:042.216
    47098C18:01018
    47098C16:1 ω7c3.215.819
    47098C18:1 ω9c21.817.769
    47098C18:2 ω6,9c/C18:0 ANTE14.717.724
    47098Unidentified4.5
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
17992-----+-----------+++/-++-+++/----

Isolation, sampling and environmental information

isolation

@refsample type
22975vaginae of women with bacterial vaginosis
47098Human vagina
17992vaginal discharge

isolation source categories

Cat1Cat2Cat3
#Infection#Disease
#Host#Human#Female
#Host Body-Site#Urogenital tract#Vagina

taxonmaps

  • @ref: 69479
  • File name: preview.99_4898.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_16139;96_2409;97_2932;98_3666;99_4898&stattab=map
  • Last taxonomy: Mobiluncus mulieris subclade
  • 16S sequence: AJ427625
  • Sequence Identity:
  • Total samples: 24050
  • soil counts: 467
  • aquatic counts: 237
  • animal counts: 23275
  • plant counts: 71

Safety information

risk assessment

  • @ref: 17992
  • biosafety level: 2
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Mobiluncus mulieris 16S-23S ribosomal RNA intergenic spacer, partial sequenceAF385928473ena585199
20218Mobiluncus mulieris partial 16S rRNA gene, strain CCUG 20071TAJ3184101404ena2052
20218Mobiluncus mulieris 16S rRNA (part.)X531871356ena585199
20218M.mulieris 16S rRNA geneX82602369ena585199
20218M.mulieris 16S rRNA geneX86004331ena585199
17992Mobiluncus mulieris partial 16S rRNA gene, strain CCUG 20071TAJ4276251404ena2052

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Mobiluncus mulieris NCTC11658GCA_900453215contigncbi2052
66792Mobiluncus mulieris ATCC 35243GCA_000160615scaffoldncbi585199
66792Mobiluncus mulieris ATCC 35243585199.3wgspatric585199
66792Mobiluncus mulieris strain NCTC116582052.9wgspatric2052
66792Mobiluncus mulieris NCTC 116582814123392draftimg2052
66792Mobiluncus mulieris ATCC 35243643886108draftimg585199

GC content

  • @ref: 22975
  • GC-content: 49-50
  • method: thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileyes65.896no
flagellatedno75.863yes
gram-positiveyes73.701no
anaerobicyes94.192no
aerobicno98.019yes
halophileno88.217no
spore-formingno93.253yes
thermophileno81.642no
glucose-utilyes76.849no
glucose-fermentyes84.64yes

External links

@ref: 17992

culture collection no.: DSM 25311, ATCC 35243, CCUG 17763, CCUG 20071, LMG 7787, NCTC 11658

straininfo link

  • @ref: 69889
  • straininfo: 3579

literature

  • topic: Pathogenicity
  • Pubmed-ID: 9258945
  • title: Antigenic profiles for the differentiation of Mobiluncus curtisii and Mobiluncus mulieris by immunoblotting technique.
  • authors: Gatti M, Aschbacher R, Cimmino C, Valentini R
  • journal: New Microbiol
  • year: 1997
  • mesh: Animals, Antibodies, Bacterial, Antigenic Variation, Antigens, Bacterial/*analysis/chemistry, Ascitic Fluid, Blotting, Western/*methods, Female, Humans, Mice, Mice, Inbred BALB C, Mobiluncus/*immunology, Molecular Weight, Vagina/microbiology

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
17992Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 25311)https://www.dsmz.de/collection/catalogue/details/culture/DSM-25311
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
22975Carol A. Spiegel,Marilyn Roberts10.1099/00207713-34-2-177Mobiluncus gen. nov., Mobiluncus curtisii subsp. curtisii sp. nov., Mobiluncus curtisii subsp. holmesii subsp. nov., and Mobiluncus mulieris sp. nov., Curved Rods from the human VaginaIJSEM 34: 177-184 1984
47098Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 20071)https://www.ccug.se/strain?id=20071
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68380Automatically annotated from API rID32A
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
69889Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID3579.1StrainInfo: A central database for resolving microbial strain identifiers