Strain identifier

BacDive ID: 2042

Type strain: Yes

Species: Cupriavidus campinensis

Strain history: CIP <- 2001, CCUG <- 2000, LMG <- M. Mergeau

NCBI tax ID(s): 151783 (species)

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General

@ref: 6878

BacDive-ID: 2042

DSM-Number: 17293

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, motile, rod-shaped

description: Cupriavidus campinensis DSM 17293 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from zinc desertified site.

NCBI tax id

  • NCBI tax id: 151783
  • Matching level: species

strain history

@refhistory
6878<- CCUG <- J. Goris, LMG Gent <- M. Mergeay
117118CIP <- 2001, CCUG <- 2000, LMG <- M. Mergeau

doi: 10.13145/bacdive2042.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Betaproteobacteria
  • order: Burkholderiales
  • family: Burkholderiaceae
  • genus: Cupriavidus
  • species: Cupriavidus campinensis
  • full scientific name: Cupriavidus campinensis (Goris et al. 2001) Vandamme and Coenye 2004

  • synonyms

    @refsynonym
    20215Wautersia campinensis
    20215Ralstonia campinensis

@ref: 6878

domain: Bacteria

phylum: Proteobacteria

class: Betaproteobacteria

order: Burkholderiales

family: Burkholderiaceae

genus: Cupriavidus

species: Cupriavidus campinensis

full scientific name: Cupriavidus campinensis (Goris et al. 2001) Vandamme and Coenye 2004

type strain: yes

Morphology

cell morphology

  • @ref: 117118
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
6878CASO AGAR (MERCK 105458) (DSMZ Medium 220)yeshttps://mediadive.dsmz.de/medium/220Name: CASO AGAR (Merck 105458) (DSMZ Medium 220) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Distilled water
33667MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
117118CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
6878positivegrowth37mesophilic
33667positivegrowth30mesophilic
56366positivegrowth28-37mesophilic
117118positivegrowth25-41
117118nogrowth5psychrophilic
117118nogrowth10psychrophilic
117118nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
56366aerobe
117118obligate aerobe

halophily

@refsaltgrowthtested relationconcentration
117118NaClpositivegrowth0-2 %
117118NaClnogrowth4 %
117118NaClnogrowth6 %
117118NaClnogrowth8 %
117118NaClnogrowth10 %

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose-builds acid from27082
68371sucrose-builds acid from17992
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371maltose-builds acid from17306
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371esculin-builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine-builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose-builds acid from16024
68371D-fructose-builds acid from15824
68371D-glucose-builds acid from17634
68371D-galactose-builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose-builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
68369malate+assimilation25115
68369adipate+assimilation17128
68369decanoate+assimilation27689
68369gluconate+assimilation24265
68369maltose-assimilation17306
68369N-acetylglucosamine-assimilation59640
68369D-mannitol-assimilation16899
68369D-mannose-assimilation16024
68369L-arabinose-assimilation30849
68369D-glucose-assimilation17634
68369gelatin-hydrolysis5291
68369esculin-hydrolysis4853
68369urea-hydrolysis16199
68369arginine-hydrolysis29016
68369D-glucose-fermentation17634
68369tryptophan-energy source27897
68369nitrate+reduction17632
68368nitrate-reduction17632
68368D-glucose-fermentation17634
68368gelatin-hydrolysis5291
68368tryptophan-energy source27897
68368urea-hydrolysis16199
68368citrate-assimilation16947
68368ornithine-degradation18257
68368lysine-degradation25094
68368arginine-hydrolysis29016
117118D-arabinose-degradation17108
117118D-fructose-degradation15824
117118D-glucose-degradation17634
117118D-mannose-degradation16024
117118D-xylose-degradation65327
117118cellobiose-degradation17057
117118lactose-degradation17716
117118maltose-degradation17306
117118salicin-degradation17814
117118sucrose-degradation17992
117118citrate-carbon source16947
117118esculin-hydrolysis4853
117118nitrate+reduction17632
117118nitrite-reduction16301
117118nitrate-respiration17632

antibiotic resistance

  • @ref: 117118
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

@refChebi-IDmetaboliteproduction
6836935581indoleno
6836817997dinitrogenno
6836816301nitriteno
6836815688acetoinno
6836835581indoleno
6836816136hydrogen sulfideno
11711835581indoleno

metabolite tests

@refChebi-IDmetaboliteindole testvoges-proskauer-testmethylred-test
6836935581indole-
6836815688acetoin-
6836835581indole-
11711817234glucose-

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase-
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
68369cytochrome oxidase+1.9.3.1
68369gelatinase-
68369beta-glucosidase-3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase-3.5.3.6
68368gelatinase-
68368tryptophan deaminase-4.1.99.1
68368urease-3.5.1.5
68368ornithine decarboxylase-4.1.1.17
68368lysine decarboxylase-4.1.1.18
68368arginine dihydrolase-3.5.3.6
68368beta-galactosidase-3.2.1.23
117118oxidase+
117118beta-galactosidase-3.2.1.23
117118alcohol dehydrogenase-1.1.1.1
117118gelatinase-
117118amylase-
117118DNase-
117118caseinase-3.4.21.50
117118catalase+1.11.1.6
117118tween esterase+
117118lecithinase-
117118lipase-
117118lysine decarboxylase-4.1.1.18
117118ornithine decarboxylase-4.1.1.17
117118protease-
117118tryptophan deaminase-
117118urease+3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
117118-+++-+----+---------

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGELGLUNO2N2
117118--------------

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
6878+-------------++++-++

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
117118-------------------------------------------------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
117118-------------------------------------------++++++++-++--------++---------------++++----+++++-++++++

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
6878zinc desertified siteLommelBelgiumBELEurope
56366Zinc desertLommelBelgiumBELEurope
117118Zinc desertified areaLommelBelgiumBELEurope

isolation source categories

  • Cat1: #Engineered
  • Cat2: #Contamination
  • Cat3: #Heavy metal

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
68781Risk group (German classification)
1171181Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Wautersia campinensis 16S rRNA gene, 23S rRNA gene and ITS1, specimen voucher LMG19282AJ783975891ena151783
6878Ralstonia campinensis strain WS2 16S ribosomal RNA gene, partial sequenceAF3120201484ena151783

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Cupriavidus campinensis LMG 19282GCA_905397395contigncbi151783
66792Cupriavidus campinensis strain LMG 19282151783.38wgspatric151783

GC content

  • @ref: 6878
  • GC-content: 66.6

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileyes87.783no
gram-positiveno98.698no
anaerobicno98.813yes
aerobicyes93.259yes
halophileno93.822no
spore-formingno94.032no
glucose-utilno72.749yes
flagellatedno60.81no
thermophileno98.784yes
glucose-fermentno90.645no

External links

@ref: 6878

culture collection no.: DSM 17293, CCUG 44526, CIP 107177, LMG 19282

straininfo link

  • @ref: 71689
  • straininfo: 13259

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny11594608Classification of metal-resistant bacteria from industrial biotopes as Ralstonia campinensis sp. nov., Ralstonia metallidurans sp. nov. and Ralstonia basilensis Steinle et al. 1998 emend.Goris J, De Vos P, Coenye T, Hoste B, Janssens D, Brim H, Diels L, Mergeay M, Kersters K, Vandamme PInt J Syst Evol Microbiol10.1099/00207713-51-5-17732001Bacterial Proteins/chemistry, Base Composition, Betaproteobacteria/*classification/*drug effects/metabolism, DNA, Ribosomal/analysis, *Drug Resistance, Bacterial, Electrophoresis, Polyacrylamide Gel, Fatty Acids/analysis, *Industrial Microbiology, Metals, Heavy/*pharmacology, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNAEnzymology
Phylogeny15545472Taxonomy of the genus Cupriavidus: a tale of lost and found.Vandamme P, Coenye TInt J Syst Evol Microbiol10.1099/ijs.0.63247-02004Bacterial Proteins/analysis/isolation & purification, Base Composition, Burkholderiaceae/*classification/genetics/physiology, DNA, Bacterial/chemistry/isolation & purification, DNA, Ribosomal/chemistry/isolation & purification, Genes, rRNA/genetics, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, Proteome/analysis/isolation & purification, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNAEnzymology

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
6878Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 17293)https://www.dsmz.de/collection/catalogue/details/culture/DSM-17293
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
33667Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/4584
56366Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 44526)https://www.ccug.se/strain?id=44526
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68368Automatically annotated from API 20E
68369Automatically annotated from API 20NE
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
71689Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID13259.1StrainInfo: A central database for resolving microbial strain identifiers
117118Curators of the CIPCollection of Institut Pasteur (CIP 107177)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107177