Strain identifier

BacDive ID: 2032

Type strain: No

Species: Cupriavidus necator

Strain Designation: H850-4LT

Strain history: <- R M. Wittich, GBF, genetic modification <- NRRL

NCBI tax ID(s): 106590 (species)

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General

@ref: 5047

BacDive-ID: 2032

DSM-Number: 13439

keywords: Bacteria, mesophilic

description: Cupriavidus necator H850-4LT is a mesophilic bacterium that was isolated from strain H850 genetically modified by introduction of the genes cbdABC of a 2-halobenzoate dioxygenase, xylXYZ of a toluate dioxygenase and tcbRCDEF of a regulated chlorocatechol pathway..

NCBI tax id

  • NCBI tax id: 106590
  • Matching level: species

strain history

  • @ref: 5047
  • history: <- R M. Wittich, GBF, genetic modification <- NRRL

doi: 10.13145/bacdive2032.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Betaproteobacteria
  • order: Burkholderiales
  • family: Burkholderiaceae
  • genus: Cupriavidus
  • species: Cupriavidus necator
  • full scientific name: Cupriavidus necator Makkar and Casida 1987

@ref: 5047

domain: Bacteria

phylum: Proteobacteria

class: Betaproteobacteria

order: Burkholderiales

family: Burkholderiaceae

genus: Cupriavidus

species: Cupriavidus necator

full scientific name: Cupriavidus necator Makkar and Casida 1987

strain designation: H850-4LT

type strain: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5047REACTIVATION WITH LIQUID MEDIUM 1 (DSMZ Medium 1a)yeshttps://mediadive.dsmz.de/medium/1aName: REACTIVATION WITH LIQUID MEDIUM 1 (DSMZ Medium 1a) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
5047MINERAL MEDIUM WITH VITAMINS (DSMZ Medium 462)yeshttps://mediadive.dsmz.de/medium/462Name: MINERAL MEDIUM (BRUNNER) WITH VITAMINS (DSMZ Medium 462) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Casein peptone 15.0 g/l Peptone 5.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Meat extract 3.0 g/l Na2HPO4 2.44 g/l KH2PO4 1.52 g/l (NH4)2SO4 0.5 g/l MgSO4 x 7 H2O 0.2 g/l CaCl2 x 2 H2O 0.05 g/l EDTA 0.005 g/l FeSO4 x 7 H2O 0.002 g/l H3BO3 0.0003 g/l CoCl2 x 6 H2O 0.0002 g/l Pyridoxamine 0.000125 g/l ZnSO4 x 7 H2O 0.0001 g/l Nicotinic acid 5e-05 g/l Vitamin B12 5e-05 g/l Na2MoO4 x 2 H2O 3e-05 g/l MnCl2 x 4 H2O 3e-05 g/l p-Aminobenzoate 2.5e-05 g/l Thiamine-HCl x 2 H2O 2.5e-05 g/l NiCl2 x 6 H2O 2e-05 g/l Calcium pantothenate 1.25e-05 g/l CuCl2 x 2 H2O 1e-05 g/l Biotin 5e-06 g/l Distilled water

culture temp

  • @ref: 5047
  • growth: positive
  • type: growth
  • temperature: 28
  • range: mesophilic

Physiology and metabolism

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate+assimilation
6836917128adipate+assimilation
6836927689decanoate+assimilation
6836924265gluconate+assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine-assimilation
6836916899D-mannitol-assimilation
6836916024D-mannose-assimilation
6836930849L-arabinose-assimilation
6836917634D-glucose-assimilation
683695291gelatin-hydrolysis
683694853esculin-hydrolysis
6836916199urea-hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate+reduction

metabolite production

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68369cytochrome oxidase+1.9.3.1
68369gelatinase-
68369beta-glucosidase-3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase-3.5.3.6

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
5047+-------------+++++++

Isolation, sampling and environmental information

isolation

  • @ref: 5047
  • sample type: strain H850 (NRRL B-15940) genetically modified by introduction of the genes cbdABC of a 2-halobenzoate dioxygenase, xylXYZ of a toluate dioxygenase and tcbRCDEF of a regulated chlorocatechol pathway.
  • geographic location: H850 was isolated from Hudson River dredge, USA

isolation source categories

  • Cat1: #Host
  • Cat2: #Microbial
  • Cat3: #Bacteria

Safety information

risk assessment

  • @ref: 5047
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

External links

@ref: 5047

culture collection no.: DSM 13439

straininfo link

  • @ref: 71679
  • straininfo: 215451

Reference

@idauthorscataloguedoi/urltitle
5047Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 13439)https://www.dsmz.de/collection/catalogue/details/culture/DSM-13439
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
68369Automatically annotated from API 20NE
71679Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID215451.1StrainInfo: A central database for resolving microbial strain identifiers