Strain identifier
BacDive ID: 2028
Type strain: 
Species: Cupriavidus necator
Strain Designation: H850
Strain history: <- NRRL <- D.L. Bedard, strain H850
NCBI tax ID(s): 106590 (species)
version 9.3 (current version)
General
@ref: 2091
BacDive-ID: 2028
DSM-Number: 5536
keywords: genome sequence, Bacteria, mesophilic, Gram-negative
description: Cupriavidus necator H850 is a mesophilic, Gram-negative bacterium that was isolated from PCB-contaminated soil.
NCBI tax id
- NCBI tax id: 106590
- Matching level: species
strain history
- @ref: 2091
- history: <- NRRL <- D.L. Bedard, strain H850
doi: 10.13145/bacdive2028.20250331.9.3
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Betaproteobacteria
- order: Burkholderiales
- family: Burkholderiaceae
- genus: Cupriavidus
- species: Cupriavidus necator
- full scientific name: Cupriavidus necator Makkar and Casida 1987
@ref: 2091
domain: Bacteria
phylum: Proteobacteria
class: Betaproteobacteria
order: Burkholderiales
family: Burkholderiaceae
genus: Cupriavidus
species: Cupriavidus necator
full scientific name: Cupriavidus necator Makkar and Casida 1987
strain designation: H850
type strain: no
Morphology
cell morphology
| @ref | gram stain | confidence |
|---|---|---|
| 125438 | negative | 97 |
| 125439 | negative | 97.5 |
Culture and growth conditions
culture medium
- @ref: 2091
- name: NUTRIENT AGAR (DSMZ Medium 1)
- growth: yes
- link: https://mediadive.dsmz.de/medium/1
- composition: Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
culture temp
- @ref: 2091
- growth: positive
- type: growth
- temperature: 30
Physiology and metabolism
oxygen tolerance
- @ref: 125439
- oxygen tolerance: obligate aerobe
- confidence: 99.2
spore formation
| @ref | spore formation | confidence |
|---|---|---|
| 125438 | no | 90.66 |
| 125439 | no | 96.5 |
Isolation, sampling and environmental information
isolation
- @ref: 2091
- sample type: PCB-contaminated soil
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Engineered | #Contamination | |
| #Environmental | #Terrestrial | #Soil |
Safety information
risk assessment
- @ref: 2091
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Cupriavidus necator H850 | GCA_009663695 | scaffold | ncbi | 53482 |
| 66792 | Cupriavidus necator H850 | 53482.3 | wgs | patric | 53482 |
| 66792 | Cupriavidus necator H850 | 2899778174 | draft | img | 106590 |
Genome-based predictions
predictions
| @ref | model | trait | description | prediction | confidence | training_data |
|---|---|---|---|---|---|---|
| 125438 | gram-positive | gram-positive | Positive reaction to Gram-staining | no | 97 | no |
| 125438 | anaerobic | anaerobic | Ability to grow under anoxygenic conditions (including facultative anaerobes) | no | 96.431 | no |
| 125438 | spore-forming | spore-forming | Ability to form endo- or exospores | no | 90.66 | no |
| 125438 | thermophile | thermophilic | Ability to grow at temperatures above or equal to 45°C | no | 97.985 | yes |
| 125438 | aerobic | aerobic | Ability to grow under oxygenic conditions (including facultative aerobes) | yes | 89.884 | no |
| 125438 | motile2+ | flagellated | Ability to perform flagellated movement | yes | 88.126 | no |
| 125439 | BacteriaNet | spore_formation | Ability to form endo- or exospores | no | 96.5 | |
| 125439 | BacteriaNet | motility | Ability to perform movement | yes | 74 | |
| 125439 | BacteriaNet | gram_stain | Reaction to gram-staining | negative | 97.5 | |
| 125439 | BacteriaNet | oxygen_tolerance | Oxygenic conditions needed for growth | obligate aerobe | 99.2 |
External links
@ref: 2091
culture collection no.: DSM 5536, NRRL B-15940
straininfo link
- @ref: 71675
- straininfo: 47940
Reference
| @id | authors | catalogue | doi/url | title |
|---|---|---|---|---|
| 2091 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 5536) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-5536 | |
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |
| 71675 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID47940.1 | StrainInfo: A central database for resolving microbial strain identifiers | |
| 125438 | Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann | 10.1101/2024.08.12.607695 | Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets | |
| 125439 | Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy | https://github.com/GenomeNet/deepG | deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 |