Strain identifier

BacDive ID: 2

Type strain: Yes

Species: Acetobacter aceti

Strain Designation: L 40

Strain history: CIP <- 1988, NCIB <- 1954, W. Werhoeven <- J. Frateur: strain L 40

NCBI tax ID(s): 887700 (strain), 435 (species)

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General

@ref: 1402

BacDive-ID: 2

DSM-Number: 3508

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative

description: Acetobacter aceti L 40 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from alcohol turned to vinegar.

NCBI tax id

NCBI tax idMatching level
887700strain
435species

strain history

@refhistory
1402<- NCIB <- M.E. Arends
67770NCIB 8621 <-- W. Verhoeven <-- J. Frateur.
115989CIP <- 1988, NCIB <- 1954, W. Werhoeven <- J. Frateur: strain L 40

doi: 10.13145/bacdive2.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Rhodospirillales
  • family: Acetobacteraceae
  • genus: Acetobacter
  • species: Acetobacter aceti
  • full scientific name: Acetobacter aceti (Pasteur 1864) Beijerinck 1898 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Mycoderma aceti

@ref: 1402

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Rhizobiales

family: Acetobacteraceae

genus: Acetobacter

species: Acetobacter aceti

full scientific name: Acetobacter aceti (Pasteur 1864) Beijerinck 1898

strain designation: L 40

type strain: yes

Morphology

cell morphology

@refgram stainconfidencecell shapemotility
69480negative99.998
115989negativerod-shapedno

colony morphology

  • @ref: 46757
  • incubation period: 1 day

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
41703MEDIUM 1 - for Acetobacter, Azotobacter, Gluconobacter, Gluconacetobacter, Mesorhizodium ciceri and Pseudomonas doudoroffiiyesDistilled water make up to (1000.000 ml);Agar (15.000 g);Yeast extract (5.000 g);Peptone (3.000 g);Mannitol (25.000 g)
1402YPM MEDIUM (DSMZ Medium 360)yesName: YPM MEDIUM (DSMZ Medium 360) Composition: Mannitol 25.0 g/l Agar 12.0 g/l Yeast extract 5.0 g/l Peptone 3.0 g/l Distilled waterhttps://mediadive.dsmz.de/medium/360
115989CIP Medium 1yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=1

culture temp

@refgrowthtypetemperaturerange
41703positivegrowth30mesophilic
1402positivegrowth25mesophilic
46757positivegrowth30mesophilic
67770positivegrowth30mesophilic
115989positivegrowth22-30
115989nogrowth10psychrophilic
115989nogrowth37mesophilic
115989nogrowth41thermophilic
115989nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
46757aerobe
115989obligate aerobe

spore formation

@refspore formationconfidence
69481no98
69480no99.999

observation

  • @ref: 67770
  • observation: quinones: Q-9

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836817632nitrate-reduction
6836828053melibiose-fermentation
6836817992sucrose-fermentation
6836862345L-rhamnose-fermentation
6836830911sorbitol-fermentation
6836817268myo-inositol-fermentation
6836817634D-glucose-fermentation
683685291gelatin-hydrolysis
6836827897tryptophan-energy source
6836816199urea-hydrolysis
6836816947citrate-assimilation
6836818257ornithine-degradation
6836825094lysine-degradation
6836829016arginine-hydrolysis
115989606565hippurate-hydrolysis
11598917632nitrate-reduction
11598916301nitrite-reduction

antibiotic resistance

  • @ref: 115989
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

@refChebi-IDmetaboliteproduction
6836817997dinitrogenno
6836816301nitriteno
6836815688acetoinno
6836835581indoleno
6836816136hydrogen sulfideno
11598935581indoleno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
6836815688acetoin-
6836835581indole-

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin+3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase-3.1.3.1
68368gelatinase-
68368tryptophan deaminase-4.1.99.1
68368urease-3.5.1.5
68368ornithine decarboxylase-4.1.1.17
68368lysine decarboxylase-4.1.1.18
68368arginine dihydrolase-3.5.3.6
68368beta-galactosidase-3.2.1.23
115989oxidase-
115989beta-galactosidase-3.2.1.23
115989alcohol dehydrogenase-1.1.1.1
115989gelatinase-
115989catalase+1.11.1.6
115989gamma-glutamyltransferase-2.3.2.2
115989lysine decarboxylase-4.1.1.18
115989ornithine decarboxylase-4.1.1.17
115989urease+3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
115989--++-+--+-++--------

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGELGLUINOSorRHASACMELNO2N2
115989-------------------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
115989+------+-----------------------+-------------------------------------------+--------------------+--

Isolation, sampling and environmental information

isolation

@refsample typesampling date
1402alcohol turned to vinegar
46757Beech-wood shavings of vinegar plant1922
67770Alcohol turned to vinegar
115989Food, Alcohol vinegar

isolation source categories

Cat1Cat2
#Engineered#Food production
#Environmental#Microbial community

taxonmaps

  • @ref: 69479
  • File name: preview.99_2615.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_29;96_152;97_827;98_2025;99_2615&stattab=map
  • Last taxonomy: Acetobacter
  • 16S sequence: X74066
  • Sequence Identity:
  • Total samples: 888
  • soil counts: 133
  • aquatic counts: 132
  • animal counts: 499
  • plant counts: 124

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
14021Risk group (German classification)
1159891Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Acetobacter aceti partial 16S-23S ITS, strain CCM 3620AJ621845807ena435
20218Acetobacter aceti gene for 16S rRNA, partial sequence, strain: JCM 7641AB6650771410ena887700
20218Acetobacter aceti gene for 16S ribosomal RNAD307681410ena887700
20218Acetobacter aceti strain LMG 1261 16S ribosomal RNA gene, partial sequenceJF7939471353ena435
20218Acetobacter aceti strain LMG 1504 16S ribosomal RNA gene, partial sequenceJF7939491351ena435
20218Acetobacter aceti DNA, 16S-23S rRNA ITS region. strain:NBRC 14818AB111902900ena887700
20218Acetobacter aceti gene for 16S rRNA, partial sequence, strain: NBRC 14818AB6806741413ena887700
20218Acetobacter aceti 16S rRNA, partial sequenceAB003967155ena887700
20218A.aceti gene for 16S rRNAX740661480ena887700

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Acetobacter aceti NBRC 14818887700.7wgspatric887700
66792Acetobacter aceti NBRC 14818887700.6wgspatric887700
66792Acetobacter aceti NBRC 14818 strain DSM 3508887700.10wgspatric887700
66792Acetobacter aceti NBRC 148182547132264draftimg887700
66792Acetobacter aceti DSM 35082784746776draftimg887700
67770Acetobacter aceti NBRC 14818GCA_000193495completencbi887700
67770Acetobacter aceti NBRC 14818GCA_000963905contigncbi887700
67770Acetobacter aceti NBRC 14818 DSM 3508GCA_004341595contigncbi887700

GC content

@refGC-contentmethod
6777057.4thermal denaturation, midpoint method (Tm)
6777058.6thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

  • trait: spore-forming
  • prediction: no
  • confidence: 98
  • training_data: no

External links

@ref: 1402

culture collection no.: DSM 3508, ATCC 15973, NCIB 8621, CCUG 18122, LMG 1261-t1, LMG 1261 1, CCTM 3043, NBRC 14818, JCM 7641, BCRC 11688, CCM 3620, CECT 298, CGMCC 1.1809, CIP 103111, ICMP 8807, IFO 14818, LMD 23.1, LMG 1261, LMG 1504, NCCB 23001, NCIMB 8621, VTT E-042567, VTT E-97828

straininfo link

  • @ref: 69687
  • straininfo: 92754

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Enzymology7991672IS1032 from Acetobacter xylinum, a new mobile insertion sequence.Iversen T, Standal R, Pedersen T, Coucheron DHPlasmid10.1006/plas.1994.10431994Amino Acid Sequence, Base Sequence, Cloning, Molecular, *DNA Transposable Elements, DNA, Bacterial/genetics, Gluconacetobacter xylinus/*genetics, Molecular Sequence Data, Sequence Alignment, Sequence HomologyGenetics
Metabolism21081762Transcriptome response to different carbon sources in Acetobacter aceti.Sakurai K, Arai H, Ishii M, Igarashi YMicrobiology (Reading)10.1099/mic.0.045906-02010Acetates/metabolism, Acetobacter/classification/genetics/*growth & development/metabolism, Bacterial Proteins/genetics/*metabolism, Carbon/chemistry/*metabolism, Citric Acid Cycle, Ethanol/metabolism, *Gene Expression Profiling, *Gene Expression Regulation, Bacterial, Glucose/metabolism, Glyoxylates/metabolism, Molecular Sequence Data, Oligonucleotide Array Sequence Analysis, Sequence Analysis, DNATranscriptome
Metabolism22153844Changes in the gene expression profile of Acetobacter aceti during growth on ethanol.Sakurai K, Arai H, Ishii M, Igarashi YJ Biosci Bioeng10.1016/j.jbiosc.2011.11.0052011Acetobacter/*enzymology/*genetics/metabolism, Alcohol Dehydrogenase/*genetics/metabolism, Ethanol/*metabolism, Gene Expression Profiling, *Gene Expression Regulation, Bacterial, Oligonucleotide Array Sequence AnalysisEnzymology
Metabolism22902276Role of the glyoxylate pathway in acetic acid production by Acetobacter aceti.Sakurai K, Yamazaki S, Ishii M, Igarashi Y, Arai HJ Biosci Bioeng10.1016/j.jbiosc.2012.07.0172012Acetates/metabolism, Acetic Acid/*metabolism/supply & distribution, Acetobacter/genetics/*metabolism, Biotechnology, Ethanol/metabolism, Glucose/metabolism, Glyoxylates/*metabolism, Isocitrate Lyase/genetics/metabolism, Malate Synthase/genetics/metabolism, Oxidation-Reduction, Transcriptome/geneticsBiotechnology
Phylogeny23194734Numerical Analysis of Phenotypic Features and Protein Gel Electrophoregrams of a Wide Variety of Acetobacter strains. Proposal for the Improvement of the Taxonomy of the Genus Acetobacter Beijerinck 1898, 215.Gossele F, Swings J, Kersters K, Pauwels P, De Ley JSyst Appl Microbiol10.1016/S0723-2020(83)80020-41983
Phylogeny26637821Acetobacter musti sp. nov., isolated from Bobal grape must.Ferrer S, Manes-Lazaro R, Benavent-Gil Y, Yepez A, Pardo IInt J Syst Evol Microbiol10.1099/ijsem.0.0008182015
Genetics33093051Complete Genome Sequence of the Acetic Acid Bacterium Acetobacter aceti NBRC 14818.Arai H, Kameya M, Ishii MMicrobiol Resour Announc10.1128/MRA.01039-202020

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
1402Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 3508)https://www.dsmz.de/collection/catalogue/details/culture/DSM-3508
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
41703Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/14873
46757Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 18122)https://www.ccug.se/strain?id=18122
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
69687Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID92754.1StrainInfo: A central database for resolving microbial strain identifiers
115989Curators of the CIPCollection of Institut Pasteur (CIP 103111)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103111