Strain identifier
BacDive ID: 1985
Type strain:
Species: Burkholderia latens
Strain history: <- CCUG <- E. Vanlaere, LMG <- S. Campana; FIRENZE 3
NCBI tax ID(s): 488446 (species)
General
@ref: 17208
BacDive-ID: 1985
DSM-Number: 23436
keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, rod-shaped
description: Burkholderia latens DSM 23436 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from sputum, patient with Cystic Fibrosis.
NCBI tax id
- NCBI tax id: 488446
- Matching level: species
strain history
- @ref: 17208
- history: <- CCUG <- E. Vanlaere, LMG <- S. Campana; FIRENZE 3
doi: 10.13145/bacdive1985.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Betaproteobacteria
- order: Burkholderiales
- family: Burkholderiaceae
- genus: Burkholderia
- species: Burkholderia latens
- full scientific name: Burkholderia latens Vanlaere et al. 2008
@ref: 17208
domain: Bacteria
phylum: Proteobacteria
class: Betaproteobacteria
order: Burkholderiales
family: Burkholderiaceae
genus: Burkholderia
species: Burkholderia latens
full scientific name: Burkholderia latens Vanlaere et al. 2008
type strain: yes
Morphology
cell morphology
- @ref: 23365
- gram stain: negative
- cell shape: rod-shaped
colony morphology
- @ref: 17208
- type of hemolysis: gamma
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
17208 | R2A MEDIUM (DSMZ Medium 830) | yes | https://mediadive.dsmz.de/medium/830 | Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water |
17208 | TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) | yes | https://mediadive.dsmz.de/medium/535 | Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
17208 | positive | growth | 30 | mesophilic |
23365 | positive | growth | 30-42 | |
60229 | positive | growth | 37 | mesophilic |
Physiology and metabolism
oxygen tolerance
@ref | oxygen tolerance |
---|---|
23365 | aerobe |
60229 | aerobe |
spore formation
- @ref: 23365
- spore formation: no
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
23365 | 17306 | maltose | - | assimilation |
23365 | 4853 | esculin | - | hydrolysis |
23365 | 17632 | nitrate | - | reduction |
23365 | 17128 | adipate | + | assimilation |
23365 | 27689 | decanoate | + | assimilation |
23365 | 16947 | citrate | + | assimilation |
23365 | 8391 | D-gluconate | + | assimilation |
23365 | 16899 | D-mannitol | + | assimilation |
23365 | 16024 | D-mannose | + | assimilation |
23365 | 17234 | glucose | + | assimilation |
23365 | 30849 | L-arabinose | + | assimilation |
23365 | 15589 | L-malate | + | assimilation |
23365 | 506227 | N-acetylglucosamine | + | assimilation |
23365 | 18401 | phenylacetate | + | assimilation |
23365 | 15963 | ribitol | + | builds acid from |
23365 | 17234 | glucose | + | builds acid from |
23365 | 17716 | lactose | + | builds acid from |
23365 | 17306 | maltose | + | builds acid from |
23365 | 17992 | sucrose | + | builds acid from |
23365 | 18222 | xylose | + | builds acid from |
68369 | 25115 | malate | + | assimilation |
68369 | 17128 | adipate | + | assimilation |
68369 | 27689 | decanoate | + | assimilation |
68369 | 24265 | gluconate | + | assimilation |
68369 | 17306 | maltose | - | assimilation |
68369 | 59640 | N-acetylglucosamine | + | assimilation |
68369 | 16899 | D-mannitol | + | assimilation |
68369 | 16024 | D-mannose | + | assimilation |
68369 | 30849 | L-arabinose | + | assimilation |
68369 | 17634 | D-glucose | + | assimilation |
68369 | 5291 | gelatin | + | hydrolysis |
68369 | 4853 | esculin | - | hydrolysis |
68369 | 16199 | urea | - | hydrolysis |
68369 | 29016 | arginine | - | hydrolysis |
68369 | 17634 | D-glucose | + | fermentation |
68369 | 27897 | tryptophan | - | energy source |
68369 | 17632 | nitrate | - | reduction |
metabolite production
- @ref: 68369
- Chebi-ID: 35581
- metabolite: indole
- production: no
metabolite tests
- @ref: 68369
- Chebi-ID: 35581
- metabolite: indole
- indole test: -
enzymes
@ref | value | activity | ec |
---|---|---|---|
17208 | catalase | + | 1.11.1.6 |
17208 | cytochrome-c oxidase | + | 1.9.3.1 |
23365 | arginine dihydrolase | - | 3.5.3.6 |
23365 | beta-galactosidase | + | 3.2.1.23 |
23365 | cytochrome oxidase | + | 1.9.3.1 |
23365 | gelatinase | + | |
23365 | lysine decarboxylase | + | 4.1.1.18 |
23365 | ornithine decarboxylase | - | 4.1.1.17 |
23365 | tryptophanase | - | 4.1.99.1 |
23365 | urease | - | 3.5.1.5 |
68369 | gelatinase | + | |
68369 | beta-glucosidase | - | 3.2.1.21 |
68369 | urease | - | 3.5.1.5 |
68369 | arginine dihydrolase | - | 3.5.3.6 |
API 20NE
@ref | NO3 | TRP | GLU_ Ferm | ADH Arg | URE | ESC | GEL | PNPG | GLU_ Assim | ARA | MNE | MAN | NAG | MAL | GNT | CAP | ADI | MLT | CIT | PAC |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
17208 | - | - | + | - | - | - | + | + | + | + | + | + | + | - | + | + | + | + | + | + |
Isolation, sampling and environmental information
isolation
@ref | sample type | country | origin.country | continent | sampling date |
---|---|---|---|---|---|
17208 | sputum, patient with Cystic Fibrosis | Italy | ITA | Europe | |
23365 | 2000 | ||||
60229 | Human sputum,cystic fibrosis | Italy | ITA | Europe | 1998-09-01 |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Infection | #Disease | #Cystic fibrosis |
#Infection | #Patient | |
#Host Body Product | #Fluids | #Sputum |
Safety information
risk assessment
- @ref: 17208
- biosafety level: 2
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 17208
- description: Burkholderia latens partial 16S rRNA gene, type strain R-5630T
- accession: AM747628
- length: 1502
- database: ena
- NCBI tax ID: 488446
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Burkholderia latens LMG 24064 | GCA_902499045 | contig | ncbi | 488446 |
66792 | Burkholderia latens CCUG 54555 | GCA_008802135 | contig | ncbi | 488446 |
66792 | Burkholderia latens strain CCUG 54555 | 488446.8 | wgs | patric | 488446 |
66792 | Burkholderia latens strain LMG 24064 | 488446.10 | wgs | patric | 488446 |
GC content
- @ref: 23365
- GC-content: 67.0
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
gram-positive | no | 98.39 | yes |
anaerobic | no | 98.855 | yes |
halophile | no | 95.539 | no |
spore-forming | no | 93.386 | yes |
glucose-util | yes | 90.944 | yes |
aerobic | yes | 91.049 | yes |
thermophile | no | 99.287 | yes |
motile | yes | 88.774 | no |
flagellated | no | 81.667 | no |
glucose-ferment | no | 88.892 | no |
External links
@ref: 17208
culture collection no.: DSM 23436, CCUG 54555, LMG 24064, FIRENZE 3
straininfo link
- @ref: 71632
- straininfo: 19767
literature
- topic: Phylogeny
- Pubmed-ID: 18599699
- title: Burkholderia latens sp. nov., Burkholderia diffusa sp. nov., Burkholderia arboris sp. nov., Burkholderia seminalis sp. nov. and Burkholderia metallica sp. nov., novel species within the Burkholderia cepacia complex.
- authors: Vanlaere E, Lipuma JJ, Baldwin A, Henry D, De Brandt E, Mahenthiralingam E, Speert D, Dowson C, Vandamme P
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijs.0.65634-0
- year: 2008
- mesh: Base Sequence, Burkholderia/chemistry/*classification/genetics, Burkholderia cepacia complex/*classification, Genes, Bacterial/genetics, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, Polymorphism, Restriction Fragment Length, RNA, Ribosomal, 16S/genetics, Rec A Recombinases/genetics, Species Specificity
- topic2: Genetics
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
17208 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 23436) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-23436 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
23365 | Elke Vanlaere, John J. LiPuma, Adam Baldwin, Deborah Henry, Evie De Brandt, Eshwar Mahenthiralingam, David Speert, Chris Dowson, Peter Vandamme | 10.1099/ijs.0.65634-0 | Burkholderia latens sp. nov., Burkholderia diffusa sp. nov., Burkholderia arboris sp. nov., Burkholderia seminalis sp. nov. and Burkholderia metallica sp. nov., novel species within the Burkholderia cepacia complex | IJSEM 58: 1580-1590 2008 | 18599699 | |
60229 | Curators of the CCUG | Culture Collection University of Gothenburg (CCUG) (CCUG 54555) | https://www.ccug.se/strain?id=54555 | |||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
68369 | Automatically annotated from API 20NE | |||||
71632 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID19767.1 | StrainInfo: A central database for resolving microbial strain identifiers |