Strain identifier

BacDive ID: 197

Type strain: Yes

Species: Arcanobacterium haemolyticum

Strain Designation: 11018

Strain history: <- K.H. Schleifer <- CCM <- A. Soucek <- NCTC <- ATCC <- A.A. Liebow, 11018 (Corynebacterium haemolyticum)

NCBI tax ID(s): 644284 (strain), 28264 (species)

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General

@ref: 8943

BacDive-ID: 197

DSM-Number: 20595

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic, Gram-positive, human pathogen

description: Arcanobacterium haemolyticum 11018 is an anaerobe, mesophilic, Gram-positive human pathogen of the family Actinomycetaceae.

NCBI tax id

NCBI tax idMatching level
28264species
644284strain

strain history

  • @ref: 8943
  • history: <- K.H. Schleifer <- CCM <- A. Soucek <- NCTC <- ATCC <- A.A. Liebow, 11018 (Corynebacterium haemolyticum)

doi: 10.13145/bacdive197.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Actinomycetales
  • family: Actinomycetaceae
  • genus: Arcanobacterium
  • species: Arcanobacterium haemolyticum
  • full scientific name: Arcanobacterium haemolyticum (ex MacLean et al. 1946) Collins et al. 1983

@ref: 8943

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Actinomycetaceae

genus: Arcanobacterium

species: Arcanobacterium haemolyticum

full scientific name: Arcanobacterium haemolyticum (ex MacLean et al. 1946) Collins et al. 1983 emend. Nouioui et al. 2018

strain designation: 11018

type strain: yes

Morphology

cell morphology

@refgram stainmotilityconfidence
22976positiveno
69480positive100

multimedia

  • @ref: 66793
  • multimedia content: EM_DSM_20595_1.jpg
  • caption: electron microscopic image
  • intellectual property rights: © HZI/Manfred Rohde

Culture and growth conditions

culture medium

  • @ref: 8943
  • name: PYG MEDIUM (MODIFIED) (DSMZ Medium 104)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/104
  • composition: Name: PYG MEDIUM (modified) (DSMZ Medium 104) Composition: Yeast extract 10.0 g/l Peptone 5.0 g/l Trypticase peptone 5.0 g/l Beef extract 5.0 g/l Glucose 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l K2HPO4 0.04 g/l KH2PO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Hemin 0.005 g/l Ethanol 0.0038 g/l Resazurin 0.001 g/l Tween 80 Vitamin K1 NaOH Distilled water

culture temp

@refgrowthtypetemperaturerange
22976positiveoptimum37.0mesophilic
8943positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
8943anaerobe
22976facultative anaerobe
69480anaerobe96.949

spore formation

@refspore formationconfidence
22976no
69481no100
69480no99.999

murein

@refmurein short keytype
8943A11.51A5alpha L-Lys-L-Lys-D-Glu
22976A11.51A5alpha L-Lys-L-Lys-D-Glu

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2297617057cellobiose-builds acid from
2297616899D-mannitol-builds acid from
2297617924D-sorbitol-builds acid from
2297665327D-xylose-builds acid from
2297628087glycogen-builds acid from
2297630849L-arabinose-builds acid from
2297662345L-rhamnose-builds acid from
2297616634raffinose-builds acid from
2297617814salicin-builds acid from
2297617992sucrose-builds acid from
2297627082trehalose-builds acid from
229765291gelatin-hydrolysis
22976606565hippurate-hydrolysis
2297617634D-glucose+builds acid from
2297616988D-ribose+builds acid from
2297617754glycerol+builds acid from
2297617716lactose+builds acid from
2297617306maltose+builds acid from
2297637684mannose+builds acid from
6838016199urea-hydrolysis
6838029016arginine-hydrolysis
6838016024D-mannose-fermentation
6838016634raffinose-fermentation
6838029985L-glutamate-degradation
6838017632nitrate-reduction
6838027897tryptophan-energy source

metabolite production

@refChebi-IDmetaboliteproduction
2297615688acetoinno
6838035581indoleno

metabolite tests

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
22976acid phosphatase+3.1.3.2
22976alkaline phosphatase+3.1.3.1
22976alpha-galactosidase-3.2.1.22
22976alpha-glucosidase+3.2.1.20
22976arginine dihydrolase-3.5.3.6
22976beta-galactosidase+3.2.1.23
22976beta-glucosidase-3.2.1.21
22976beta-glucuronidase-3.2.1.31
22976esterase lipase (C 8)+
22976leucine arylamidase-3.4.11.1
22976N-acetyl-beta-glucosaminidase+3.2.1.52
22976nitrate reductase-1.7.99.4
22976pyrazinamidase+3.5.1.B15
22976pyrrolidonyl arylamidase-3.4.19.3
22976urease-3.5.1.5
68380serine arylamidase-
68380glutamyl-glutamate arylamidase-
68380histidine arylamidase-
68380glycin arylamidase-
68380alanine arylamidase-3.4.11.2
68380tyrosine arylamidase-
68380pyrrolidonyl arylamidase-3.4.19.3
68380leucine arylamidase-3.4.11.1
68380phenylalanine arylamidase-
68380proline-arylamidase+3.4.11.5
68380L-arginine arylamidase+
68380tryptophan deaminase-4.1.99.1
68380alpha-fucosidase-3.2.1.51
68380glutamate decarboxylase-4.1.1.15
68380beta-glucuronidase-3.2.1.31
68380alpha-arabinosidase-3.2.1.55
68380beta-glucosidase-3.2.1.21
68380alpha-glucosidase-3.2.1.20
68380beta-galactosidase+3.2.1.23
68380alpha-galactosidase-3.2.1.22
68380arginine dihydrolase-3.5.3.6
68380urease-3.5.1.5

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
8943---++/-----+------++++---------
8943---+-------------++----------

Safety information

risk assessment

  • @ref: 8943
  • pathogenicity human: yes
  • pathogenicity animal: yes
  • biosafety level: 2
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 20218
  • description: Arcanobacterium haemolyticum strain ATCC 9345 16S ribosomal RNA gene and 16S-23S ribosomal RNA intergenic spacer, partial sequence
  • accession: EU194564
  • length: 506
  • database: ena
  • NCBI tax ID: 644284

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Arcanobacterium haemolyticum NCTC8452GCA_900475915completencbi28264
66792Arcanobacterium haemolyticum DSM 20595GCA_000092365completencbi644284
66792Arcanobacterium haemolyticum DSM 20595644284.3completepatric644284
66792Arcanobacterium haemolyticum strain NCTC845228264.12completepatric28264
66792Arcanobacterium haemolyticum CCM, DSM 20595646564505completeimg644284
66792Arcanobacterium haemolyticum NCTC 84522808606785completeimg28264

GC content

  • @ref: 22976
  • GC-content: 50-57
  • method: high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileno92.712no
flagellatedno97.581no
gram-positiveyes95.169yes
anaerobicno85.424no
aerobicno93.816yes
halophileno84.072no
spore-formingno96.403no
thermophileno94.193no
glucose-utilyes86.34no
glucose-fermentyes79.485no

External links

@ref: 8943

culture collection no.: DSM 20595, ATCC 9345, CCM 5947, NCTC 8452

straininfo link

  • @ref: 69880
  • straininfo: 8172

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny7119737Reclassification of 'Corynebacterium haemolyticum' (MacLean, Liebow & Rosenberg) in the genus Arcanobacterium gen.nov. as Arcanobacterium haemolyticum nom.rev., comb.nov.Collins MD, Jones D, Schofield GMJ Gen Microbiol10.1099/00221287-128-6-12791982Corynebacterium/*classification, Terminology as Topic
Pathogenicity9440197Isolation, in vitro antimicrobial susceptibility and penicillin tolerance of Arcanobacterium haemolyticum in a Turkish university hospital.Arikan S, Erguven S, Gunalp AZentralbl Bakteriol10.1016/s0934-8840(97)80051-51997Actinomycetaceae/*drug effects/isolation & purification, Actinomycetales Infections/*microbiology, Adolescent, Adult, Child, Female, Hospitals, Humans, Male, Microbial Sensitivity Tests, *Penicillin Resistance, Pharyngitis/*microbiology, Tonsillitis/*microbiology, TurkeyPhylogeny
Phylogeny16585707Arcanobacterium bialowiezense sp. nov. and Arcanobacterium bonasi sp. nov., isolated from the prepuce of European bison bulls (Bison bonasus) suffering from balanoposthitis, and emended description of the genus Arcanobacterium Collins et al. 1983.Lehnen A, Busse HJ, Frolich K, Krasinska M, Kampfer P, Speck SInt J Syst Evol Microbiol10.1099/ijs.0.63923-02006Actinomycetaceae/chemistry/*classification/genetics/isolation & purification, Actinomycetales Infections/*microbiology, Animals, Bacterial Typing Techniques, Bison/microbiology, Male, Molecular Sequence Data, Penis/microbiology, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/analysis/geneticsGenetics
Genetics21304742Complete genome sequence of Arcanobacterium haemolyticum type strain (11018).Yasawong M, Teshima H, Lapidus A, Nolan M, Lucas S, Glavina Del Rio T, Tice H, Cheng JF, Bruce D, Detter C, Tapia R, Han C, Goodwin L, Pitluck S, Liolios K, Ivanova N, Mavromatis K, Mikhailova N, Pati A, Chen A, Palaniappan K, Land M, Hauser L, Chang YJ, Jeffries CD, Rohde M, Sikorski J, Pukall R, Goker M, Woyke T, Bristow J, Eisen JA, Markowitz V, Hugenholtz P, Kyrpides NC, Klenk HPStand Genomic Sci10.4056/sigs.11230722010
Phylogeny24490344Properties of an Arcanobacterium haemolyticum strain isolated from a donkey.Sammra O, Balbutskaya A, Nagib S, Alber J, Lammler C, Abdulmawjood A, Timke M, Kostrzewa M, Prenger-Berninghoff EBerl Munch Tierarztl Wochenschr2014Actinomycetales Infections/*microbiology, Animals, Arcanobacterium/*genetics/isolation & purification/*pathogenicity, DNA, Bacterial/genetics, Equidae/*microbiology, Genotype, Nasal Cavity/microbiology, Phenotype, RNA, Ribosomal, 16S/genetics, Spectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationGenetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8943Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20595)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20595
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
22976A. F. Yassin,H. Hupfer,C. Siering,P. Schumann10.1099/ijs.0.020032-0Comparative chemotaxonomic and phylogenetic studies on the genus Arcanobacterium Collins et al. 1982 emend. Lehnen et al. 2006: proposal for Trueperella gen. nov. and emended description of the genus ArcanobacteriumIJSEM 61: 1265-1274 201120622055
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66793Mukherjee et al.10.1038/nbt.3886GEBA: 1,003 reference genomes of bacterial and archaeal isolates expand coverage of the tree of life 35: 676-683 201728604660
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
68380Automatically annotated from API rID32A
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
69880Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID8172.1StrainInfo: A central database for resolving microbial strain identifiers