Strain identifier

BacDive ID: 196

Type strain: Yes

Species: Actinomyces weissii

Strain Designation: 2298, 2298/06

Strain history: CIP <- 2011, P. Kampfer, Giessen Univ., Giessen, Germany: strain 2298

NCBI tax ID(s): 675090 (species)

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General

@ref: 17796

BacDive-ID: 196

DSM-Number: 24894

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic, Gram-positive, rod-shaped, animal pathogen

description: Actinomyces weissii 2298 is an anaerobe, mesophilic, Gram-positive animal pathogen that was isolated from oral cavity, dog.

NCBI tax id

  • NCBI tax id: 675090
  • Matching level: species

strain history

@refhistory
383852011, P. Kampfer, Inst. für Angewandte Mikrobiologie, Giessen, Germany: strain 2298
17796<- P. Kämpfer, Justus-Liebig-Universität, Giessen, Germany; strain 2298 <- M. Hijazin
123935CIP <- 2011, P. Kampfer, Giessen Univ., Giessen, Germany: strain 2298

doi: 10.13145/bacdive196.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Actinomycetales
  • family: Actinomycetaceae
  • genus: Actinomyces
  • species: Actinomyces weissii
  • full scientific name: Actinomyces weissii Hijazin et al. 2012

@ref: 17796

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Actinomycetaceae

genus: Actinomyces

species: Actinomyces weissii

full scientific name: Actinomyces weissii Hijazin et al. 2012

strain designation: 2298, 2298/06

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
30418positive6 µm1.25 µmrod-shapedno
69480no93.5
69480positive100
123935positiverod-shapedno

pigmentation

  • @ref: 30418
  • production: yes

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
38385MEDIUM 6 - Columbia agar with 10 % horse bloodyesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml)
17796BHI MEDIUM (DSMZ Medium 215)yesName: BHI MEDIUM (DSMZ Medium 215) Composition: Brain heart infusion 37.0 g/l Distilled waterhttps://mediadive.dsmz.de/medium/215
17796PYG MEDIUM (MODIFIED) (DSMZ Medium 104)yesName: PYG MEDIUM (modified) (DSMZ Medium 104) Composition: Yeast extract 10.0 g/l Peptone 5.0 g/l Trypticase peptone 5.0 g/l Beef extract 5.0 g/l Glucose 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l K2HPO4 0.04 g/l KH2PO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Hemin 0.005 g/l Ethanol 0.0038 g/l Resazurin 0.001 g/l Tween 80 Vitamin K1 NaOH Distilled waterhttps://mediadive.dsmz.de/medium/104
123935CIP Medium 6yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=6

culture temp

@refgrowthtypetemperaturerange
17796positivegrowth37mesophilic
30418positivegrowth35mesophilic
38385positivegrowth37mesophilic
62610positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
17796anaerobe
17796microaerophile
30418microaerophile
62610anaerobe
69480anaerobe96.121
123935facultative anaerobe

spore formation

@refspore formationconfidence
30418no
69481yes98
69480no99.964

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3041817234glucose+carbon source
3041828087glycogen+carbon source
3041817716lactose+carbon source
3041817306maltose+carbon source
3041817992sucrose+carbon source
304184853esculin+hydrolysis
12393517632nitrate-reduction
12393516301nitrite-reduction

metabolite production

  • @ref: 123935
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
30418alkaline phosphatase+3.1.3.1
30418catalase+1.11.1.6
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase-3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)+
68382alkaline phosphatase-3.1.3.1
123935oxidase-
123935catalase+1.11.1.6
123935urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
123935--+--++----+-+-+-+--

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentsampling date
17796oral cavity, dogGiessenGermanyDEUEurope
62610Dog,oral cavityGiessenGermanyDEUEurope2006
123935Animal, Dog, oral cavityGiessen, HessenGermanyDEUEurope

isolation source categories

Cat1Cat2Cat3
#Host#Mammals#Canidae (Dog)
#Host Body-Site#Oral cavity and airways

Safety information

risk assessment

@refpathogenicity animalbiosafety levelbiosafety level comment
17796yes2Risk group (German classification)
1239352Risk group (French classification)

Sequence information

16S sequences

  • @ref: 17796
  • description: Actinomyces weissii partial 16S rRNA gene, strain 2298
  • accession: FN552454
  • length: 1379
  • database: ena
  • NCBI tax ID: 675090

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Actinomyces weissii CCUG 61299GCA_016598775completencbi675090
66792Actinomyces weissii strain CCUG 61299675090.3completepatric675090

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes98no
flagellatedno97.124yes
gram-positiveyes95.009no
anaerobicno63.818no
aerobicno97.661no
halophileno84.072no
spore-formingno93.304yes
thermophileno95.847yes
glucose-utilyes86.176yes
motileno93.275yes
glucose-fermentyes76.469no

External links

@ref: 17796

culture collection no.: DSM 24894, CCM 7951, CCUG 61299, CIP 110333, LMG 26472

straininfo link

  • @ref: 69879
  • straininfo: 377658

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny21948094Actinomyces weissii sp. nov., isolated from dogs.Hijazin M, Alber J, Lammler C, Kampfer P, Glaeser SP, Busse HJ, Kassmannhuber J, Prenger-Berninghoff E, Fornges T, Hassan AA, Abdulmawjood A, Zschock MInt J Syst Evol Microbiol10.1099/ijs.0.035626-02011Actinomyces/*classification/genetics/*isolation & purification, Animals, Bacterial Typing Techniques, DNA, Bacterial/genetics, Dogs/*microbiology, Fatty Acids/analysis, Molecular Sequence Data, Phospholipids/analysis, *Phylogeny, Polyamines/analysis, Quinones/analysis, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNAGenetics
Phylogeny34252022Characterization of isolates of members of the genus Actinomyces from Marmota himalayana: description of Actinomyces faecalis sp. nov., Actinomyces respiraculi sp. nov., and Actinomyces trachealis sp. nov.Zhou J, Zhang S, Zhang G, Yang J, Lai XH, Pu J, Jin D, Lu S, Huang Y, Zhu W, Huang Y, Xu M, Lei W, Cheng Y, Liu L, Xu JInt J Syst Evol Microbiol10.1099/ijsem.0.0048752021Actinomyces/*classification/isolation & purification, Animals, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Marmota/*microbiology, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, TibetTranscriptome

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
17796Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 24894)https://www.dsmz.de/collection/catalogue/details/culture/DSM-24894
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
30418Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2675428776041
38385Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/8181
62610Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 61299)https://www.ccug.se/strain?id=61299
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
69879Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID377658.1StrainInfo: A central database for resolving microbial strain identifiers
123935Curators of the CIPCollection of Institut Pasteur (CIP 110333)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20110333