Strain identifier

BacDive ID: 195

Type strain: Yes

Species: Schaalia naturae

Strain Designation: BL-79

Strain history: <- NRRL; NRRL-B-24670

NCBI tax ID(s): 635203 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 18274

BacDive-ID: 195

DSM-Number: 26713

keywords: mesophilic, anaerobe, Bacteria, 16S sequence

description: Schaalia naturae BL-79 is an anaerobe, mesophilic Actinobacterium that was isolated from chlorinated solvent contaminated groundwater.

NCBI tax id

  • NCBI tax id: 635203
  • Matching level: species

strain history: <- NRRL; NRRL-B-24670

doi: 10.13145/bacdive195.20211221.6

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinobacteria
  • domain: Bacteria
  • phylum: Actinobacteria
  • class: Actinobacteria
  • order: Actinomycetales
  • family: Actinomycetaceae
  • genus: Schaalia
  • species: Schaalia naturae
  • full scientific name: Schaalia naturae (Rao et al. 2012) Nouioui et al. 2018
  • synonyms

    • @ref: 20215
    • synonym: Actinomyces naturae

@ref: 18274

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Actinomycetaceae

genus: Schaalia

species: Schaalia naturae

full scientific name: Schaalia naturae (Rao et al. 2012) Nouioui et al. 2018

strain designation: BL-79

type strain: yes

Culture and growth conditions

culture medium

  • @ref: 18274
  • name: PYG MEDIUM (MODIFIED) (DSMZ Medium 104)
  • growth: yes
  • link: https://bacmedia.dsmz.de/medium/104
  • composition: Name: PYG MEDIUM (modified) (DSMZ Medium 104) Composition: Yeast extract 10.0 g/l Peptone 5.0 g/l Trypticase peptone 5.0 g/l Beef extract 5.0 g/l Glucose 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l K2HPO4 0.04 g/l KH2PO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Hemin 0.005 g/l Ethanol 0.0038 g/l Resazurin 0.001 g/l Tween 80 Vitamin K1 NaOH Distilled water

culture temp

@refgrowthtypetemperaturerange
18274positivegrowth37mesophilic
61062positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
18274anaerobe
61062anaerobe
61062microaerophile

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6838027897tryptophan-energy source
6838017632nitrate-reduction
6838029985L-glutamate-degradation
6838016634raffinose-fermentation
6838016024D-mannose+fermentation
6838029016arginine-hydrolysis
6838016199urea-hydrolysis

metabolite production

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68380glutamyl-glutamate arylamidase-
68380glycin arylamidase-
68380alanine arylamidase-3.4.11.2
68380tyrosine arylamidase+
68380pyrrolidonyl arylamidase-3.4.19.3
68380leucine arylamidase+3.4.11.1
68380phenylalanine arylamidase+
68380leucyl glycin arylamidase-3.4.11.1
68380proline-arylamidase+3.4.11.5
68380L-arginine arylamidase+
68380alkaline phosphatase-3.1.3.1
68380tryptophan deaminase-4.1.99.1
68380alpha-fucosidase-3.2.1.51
68380glutamate decarboxylase-4.1.1.15
68380N-acetyl-beta-glucosaminidase-3.2.1.52
68380beta-glucuronidase-3.2.1.31
68380beta-Galactosidase 6-phosphate-
68380beta-galactosidase+3.2.1.23
68380alpha-galactosidase+3.2.1.22
68380arginine dihydrolase-3.5.3.6
68380urease-3.5.1.5

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
18274--++-+/-+/-+/---+------++-++-+--+/--+/-

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentsampling date
18274chlorinated solvent contaminated groundwaterBaton Rouge, LA; PetroProcessors of Louisiana, IncUSAUSANorth America
61062GroundwaterLouisiana,Baton RougeUSAUSANorth America2005

isolation source categories

Cat1Cat2Cat3
#Engineered#Contamination
#Environmental#Aquatic#Groundwater

Safety information

risk assessment

  • @ref: 18274
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 18274
  • description: Actinomyces naturae 16S ribosomal RNA gene, partial sequence
  • accession: FJ234421
  • length: 1463
  • database: ena
  • NCBI tax ID: 635203

GC content

  • @ref: 18274
  • GC-content: 69.8

External links

@ref: 18274

culture collection no.: DSM 26713, CCUG 56698, NRRL B-24670

straininfo link

  • @ref: 20218
  • passport: http://www.straininfo.net/strains/869324

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
18274Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 26713)https://www.dsmz.de/collection/catalogue/details/culture/DSM-26713
20215D.Gleim, M.Kracht, N.Weiss et. al.http://www.dsmz.de/bacterial-diversity/prokaryotic-nomenclature-up-to-date.htmlProkaryotic Nomenclature Up-to-date - compilation of all names of Bacteria and Archaea, validly published according to the Bacteriological Code since 1. Jan. 1980, and validly published nomenclatural changes since
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
61062Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 56698)https://www.ccug.se/strain?id=56698