Strain identifier

BacDive ID: 1949

Type strain: No

Species: Burkholderia dolosa

Strain Designation: PC534, R-3207

Strain history: <- CCUG <- LMG <- T. Coenye; R-3207 <- J. J. LiPuma; PC534

NCBI tax ID(s): 152500 (species)

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General

@ref: 18163

BacDive-ID: 1949

DSM-Number: 26123

keywords: genome sequence, Bacteria, aerobe, mesophilic

description: Burkholderia dolosa PC534 is an aerobe, mesophilic bacterium that was isolated from Human sputum,cystic fibrosis.

NCBI tax id

  • NCBI tax id: 152500
  • Matching level: species

strain history

  • @ref: 18163
  • history: <- CCUG <- LMG <- T. Coenye; R-3207 <- J. J. LiPuma; PC534

doi: 10.13145/bacdive1949.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Betaproteobacteria
  • order: Burkholderiales
  • family: Burkholderiaceae
  • genus: Burkholderia
  • species: Burkholderia dolosa
  • full scientific name: Burkholderia dolosa Vermis et al. 2004

@ref: 18163

domain: Bacteria

phylum: Proteobacteria

class: Betaproteobacteria

order: Burkholderiales

family: Burkholderiaceae

genus: Burkholderia

species: Burkholderia dolosa

full scientific name: Burkholderia dolosa Vermis et al. 2004

strain designation: PC534, R-3207

type strain: no

Morphology

colony morphology

  • @ref: 18163
  • type of hemolysis: gamma
  • incubation period: 1-2 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
18163COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
18163CASO AGAR (MERCK 105458) (DSMZ Medium 220)yeshttps://mediadive.dsmz.de/medium/220Name: CASO AGAR (Merck 105458) (DSMZ Medium 220) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
18163positivegrowth37mesophilic
18163positivegrowth30mesophilic
56657positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 56657
  • oxygen tolerance: aerobe

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate+assimilation
6836917128adipate+assimilation
6836927689decanoate+assimilation
6836924265gluconate+assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine+assimilation
6836916899D-mannitol+assimilation
6836916024D-mannose+assimilation
6836930849L-arabinose+assimilation
6836917634D-glucose+assimilation
683695291gelatin-hydrolysis
683694853esculin-hydrolysis
6836916199urea-hydrolysis
6836929016arginine-hydrolysis
6836927897tryptophan-energy source
6836917632nitrate+reduction

metabolite production

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
18163catalase+1.11.1.6
18163cytochrome-c oxidase+1.9.3.1
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)+
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
68369cytochrome oxidase+1.9.3.1
68369gelatinase-
68369beta-glucosidase-3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase-3.5.3.6

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
56657-+++++----++-----+--

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
18163+-+/-----+/-+++++-+++++++
56657+-+----++++++-+++++++

Isolation, sampling and environmental information

isolation

  • @ref: 56657
  • sample type: Human sputum,cystic fibrosis
  • country: USA
  • origin.country: USA
  • continent: North America

isolation source categories

Cat1Cat2Cat3
#Infection#Disease#Cystic fibrosis
#Host#Human
#Infection#Patient
#Host Body Product#Fluids#Sputum

Safety information

risk assessment

  • @ref: 18163
  • biosafety level: 2
  • biosafety level comment: Risk group (German classification)

Sequence information

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Burkholderia dolosa strain FDAARGOS_1544152500.67completepatric152500
66792Burkholderia dolosa strain FDAARGOS_1544152500.54completepatric152500

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileyes89.035no
gram-positiveno98.126no
anaerobicno98.845yes
aerobicyes90.064yes
halophileno94.351no
spore-formingno93.399no
glucose-utilyes89.366yes
flagellatedno88.192no
glucose-fermentno89.721no
thermophileno98.702no

External links

@ref: 18163

culture collection no.: DSM 26123, CCUG 45123, LMG 18942

straininfo link

  • @ref: 71594
  • straininfo: 13004

Reference

@idauthorscataloguedoi/urltitle
18163Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 26123)https://www.dsmz.de/collection/catalogue/details/culture/DSM-26123
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
56657Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 45123)https://www.ccug.se/strain?id=45123
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68369Automatically annotated from API 20NE
68382Automatically annotated from API zym
71594Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID13004.1StrainInfo: A central database for resolving microbial strain identifiers