Strain identifier

BacDive ID: 1942

Type strain: Yes

Species: Burkholderia thailandensis

Strain Designation: E264

Strain history: CIP <- 1999, D.E. Woods, Univ. of Calgary Health Sci. Centre, Canada <- 1995, N.J. White: strain E264

NCBI tax ID(s): 271848 (strain), 57975 (species)

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General

@ref: 4988

BacDive-ID: 1942

DSM-Number: 13276

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, motile, rod-shaped

description: Burkholderia thailandensis E264 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from environmental sample.

NCBI tax id

NCBI tax idMatching level
271848strain
57975species

strain history

@refhistory
4988<- D. E. Woods; E264
123492CIP <- 1999, D.E. Woods, Univ. of Calgary Health Sci. Centre, Canada <- 1995, N.J. White: strain E264

doi: 10.13145/bacdive1942.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Betaproteobacteria
  • order: Burkholderiales
  • family: Burkholderiaceae
  • genus: Burkholderia
  • species: Burkholderia thailandensis
  • full scientific name: Burkholderia thailandensis Brett et al. 1998

@ref: 4988

domain: Bacteria

phylum: Proteobacteria

class: Betaproteobacteria

order: Burkholderiales

family: Burkholderiaceae

genus: Burkholderia

species: Burkholderia thailandensis

full scientific name: Burkholderia thailandensis Brett et al. 1998

strain designation: E264

type strain: yes

Morphology

cell morphology

  • @ref: 123492
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: yes

colony morphology

  • @ref: 4988
  • type of hemolysis: gamma
  • incubation period: 1-2 days

pigmentation

  • @ref: 123492
  • production: no
  • name: Pyocyanin

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
4988NUTRIENT AGAR (DSMZ Medium 1)yeshttps://mediadive.dsmz.de/medium/1Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
4988TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)yeshttps://mediadive.dsmz.de/medium/535Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
4988CASO AGAR (MERCK 105458) (DSMZ Medium 220)yeshttps://mediadive.dsmz.de/medium/220Name: CASO AGAR (Merck 105458) (DSMZ Medium 220) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Distilled water
39485MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
123492CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
4988positivegrowth28mesophilic
39485positivegrowth30mesophilic
58359positivegrowth30-37mesophilic
123492positivegrowth25-41
123492nogrowth5psychrophilic
123492nogrowth10psychrophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
58359aerobe
123492obligate aerobe

halophily

@refsaltgrowthtested relationconcentration
123492NaClpositivegrowth0-4 %
123492NaClnogrowth6 %
123492NaClnogrowth8 %
123492NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate+assimilation
6836917128adipate+assimilation
6836927689decanoate+assimilation
6836924265gluconate+assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine+assimilation
6836916899D-mannitol+assimilation
6836916024D-mannose+assimilation
6836930849L-arabinose+assimilation
6836917634D-glucose+assimilation
683695291gelatin+hydrolysis
6836916199urea-hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
12349216947citrate+carbon source
1234924853esculin-hydrolysis
12349217632nitrate+reduction
12349216301nitrite+reduction
12349217632nitrate+respiration

antibiotic resistance

  • @ref: 123492
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

@refChebi-IDmetaboliteproduction
6836935581indoleno
12349235581indoleno

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
4988cytochrome-c oxidase+1.9.3.1
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)+
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
68369cytochrome oxidase+1.9.3.1
68369gelatinase+
68369urease-3.5.1.5
123492oxidase+
123492beta-galactosidase-3.2.1.23
123492alcohol dehydrogenase+1.1.1.1
123492gelatinase+
123492amylase-
123492DNase-
123492caseinase+3.4.21.50
123492catalase+1.11.1.6
123492tween esterase+
123492lecithinase+
123492lipase+
123492lysine decarboxylase-4.1.1.18
123492ornithine decarboxylase-4.1.1.17
123492protease+
123492tryptophan deaminase-
123492urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
123492-+++++----++--------

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
4988+--+-+/-+-+++++-+++++++
4988+--+--+-+++++-+++++++
4988------+-+++++-+++++/-++
4988+--+-+/-+-+++++-++++++
4988+--+-++-+++++-+++++++

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
123492+++++--+-+-----++-++++--+-++++++++--++-----+-----+++-+--++-+++++++-+---+++-+++-++++++++-+++++++++++

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentsampling dateisolation date
4988environmental sampleThailandTHAAsia
58359Soil,rice fieldThailandTHAAsia1995
123492Environment, Rice field soilThailandTHAAsia1995

isolation source categories

  • Cat1: #Environmental

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
49882Risk group (German classification)
1234921Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Burkholderia thailandensis strain E264 16S ribosomal RNA gene, partial sequenceEF5352351541ena271848
20218Burkholderia thailandensis strain BD10-00323 16S ribosomal RNA gene, partial sequenceKF4449061459ena57975
4988Burkholderia thailandensis strain E264 16S ribosomal RNA gene, complete sequenceU918381488ena271848

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Burkholderia thailandensis E264GCA_003568605completencbi271848
66792Burkholderia thailandensis E264 E264; ATCC 700388GCA_000012365completencbi271848
66792Burkholderia thailandensis E264 ATCC 700388GCA_000152285chromosomencbi271848
66792Burkholderia thailandensis ATCC 700388271848.8wgspatric271848
66792Burkholderia thailandensis Bt4441164.8wgspatric441164
66792Burkholderia thailandensis E264271848.6completepatric271848
66792Burkholderia thailandensis E264271848.56completepatric271848
66792Burkholderia thailandensis E264, ATCC 700388645058826draftimg271848
66792Burkholderia thailandensis E2642900857987completeimg271848
66792Burkholderia thailandensis E264, ATCC 700388637000052completeimg271848

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileyes84.722no
flagellatedno78.626no
gram-positiveno98.192no
anaerobicno97.357yes
aerobicyes85.543yes
halophileno94.25no
spore-formingno93.323no
glucose-utilyes91.509yes
thermophileno99.481yes
glucose-fermentno87.958no

External links

@ref: 4988

culture collection no.: CCUG 48851, CIP 106301, DSM 13276, ATCC 700388

straininfo link

  • @ref: 71587
  • straininfo: 46232

literature

topicPubmed-IDtitleauthorsjournalyearmeshtopic2DOI
Pathogenicity24189672Colonial morphotypes and biofilm forming ability of Burkholderia pseudomallei.Koh SF, Tay ST, Puthucheary SDTrop Biomed2013Bacteriological Techniques/methods, Biofilms/*growth & development, Blood/microbiology, Burkholderia pseudomallei/growth & development/isolation & purification/*physiology, Culture Media/chemistry, Humans, Melioidosis/microbiology, Phenotype, Staining and Labeling/methods, Suppuration/microbiologyPhenotype
Pathogenicity28065676Rhamnolipids from non-pathogenic Burkholderia thailandensis E264: Physicochemical characterization, antimicrobial and antibiofilm efficacy against oral hygiene related pathogens.Elshikh M, Funston S, Chebbi A, Ahmed S, Marchant R, Banat IMN Biotechnol2017Biofilms/drug effects, Biotechnology, Burkholderia/*metabolism, Chemical Phenomena, Glycolipids/chemistry/*metabolism/pharmacology, Humans, Microbial Sensitivity Tests, Mouth/drug effects/microbiology, Oral Hygiene, Reactive Oxygen Species/metabolism, Surface-Active Agents/chemistry/metabolism/pharmacologyMetabolism10.1016/j.nbt.2016.12.009
33361318Exometabolite Dynamics over Stationary Phase Reveal Strain-Specific Responses.Chodkowski JL, Shade AmSystems202010.1128/mSystems.00493-20
35631971Characterization of the Thermostable Biosurfactant Produced by Burkholderia thailandensis DSM 13276.Gil CV, Rebocho AT, Esmail A, Sevrin C, Grandfils C, Torres CAV, Reis MAM, Freitas FPolymers (Basel)202210.3390/polym14102088

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
4988Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 13276)https://www.dsmz.de/collection/catalogue/details/culture/DSM-13276
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
39485Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/18416
58359Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 48851)https://www.ccug.se/strain?id=48851
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68369Automatically annotated from API 20NE
68382Automatically annotated from API zym
71587Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID46232.1StrainInfo: A central database for resolving microbial strain identifiers
123492Curators of the CIPCollection of Institut Pasteur (CIP 106301)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20106301