Strain identifier

BacDive ID: 1941

Type strain: Yes

Species: Burkholderia multivorans

Strain Designation: Cepa 002

Strain history: CIP <- 1998, LMG <- S. Lauwers <- M. Struelens

NCBI tax ID(s): 985079 (strain), 87883 (species)

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General

@ref: 4980

BacDive-ID: 1941

DSM-Number: 13243

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, motile, rod-shaped

description: Burkholderia multivorans Cepa 002 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from patient suffering from cystic fibrosis.

NCBI tax id

NCBI tax idMatching level
87883species
985079strain

strain history

@refhistory
4980<- S. Lauwers (Pseudomonas cepacia) <- M. Struelens
121175CIP <- 1998, LMG <- S. Lauwers <- M. Struelens

doi: 10.13145/bacdive1941.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Betaproteobacteria
  • order: Burkholderiales
  • family: Burkholderiaceae
  • genus: Burkholderia
  • species: Burkholderia multivorans
  • full scientific name: Burkholderia multivorans Vandamme et al. 1997

@ref: 4980

domain: Bacteria

phylum: Proteobacteria

class: Betaproteobacteria

order: Burkholderiales

family: Burkholderiaceae

genus: Burkholderia

species: Burkholderia multivorans

full scientific name: Burkholderia multivorans Vandamme et al. 1997

strain designation: Cepa 002

type strain: yes

Morphology

cell morphology

  • @ref: 121175
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: yes

colony morphology

  • @ref: 4980
  • type of hemolysis: gamma
  • incubation period: 1-2 days

pigmentation

  • @ref: 121175
  • production: no
  • name: Pyocyanin

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
4980NUTRIENT AGAR (DSMZ Medium 1)yeshttps://mediadive.dsmz.de/medium/1Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
35830MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
4980TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
4980COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
121175CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
4980positivegrowth28mesophilic
35830positivegrowth30mesophilic
51924positivegrowth37mesophilic
4980positivegrowth30mesophilic
121175positivegrowth25-41
121175nogrowth5psychrophilic
121175nogrowth10psychrophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
51924aerobe
121175obligate aerobe

halophily

@refsaltgrowthtested relationconcentration
121175NaClpositivegrowth0-6 %
121175NaClnogrowth8 %
121175NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate+assimilation
6836917128adipate+assimilation
6836927689decanoate+assimilation
6836924265gluconate+assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine+assimilation
6836916899D-mannitol+assimilation
6836916024D-mannose+assimilation
6836930849L-arabinose+assimilation
6836917634D-glucose+assimilation
683695291gelatin-hydrolysis
6836916199urea-hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
12117516947citrate+carbon source
1211754853esculin-hydrolysis
12117517632nitrate+reduction
12117516301nitrite-reduction
12117517632nitrate-respiration

antibiotic resistance

  • @ref: 121175
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

@refChebi-IDmetaboliteproduction
6836935581indoleno
12117535581indoleno

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
4980catalase+1.11.1.6
4980cytochrome-c oxidase+1.9.3.1
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)+
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
68369cytochrome oxidase+1.9.3.1
68369gelatinase-
68369urease-3.5.1.5
68369arginine dihydrolase-3.5.3.6
121175oxidase+
121175beta-galactosidase+3.2.1.23
121175alcohol dehydrogenase-1.1.1.1
121175gelatinase-
121175amylase-
121175DNase-
121175caseinase-3.4.21.50
121175catalase+1.11.1.6
121175tween esterase+
121175lecithinase+
121175lipase-
121175lysine decarboxylase-4.1.1.18
121175ornithine decarboxylase-4.1.1.17
121175tryptophan deaminase-
121175urease+3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
121175-+++++----++----+---

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
4980+------++++++-+++++++
4980+----+/--++++++-+++++++
4980-------++++++-+++++++

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
121175+++++--------+-++-++++--+-++++++++--++-+---+++--+++++++++-++++++++++--+++++-+++++++++++-+++++++++++

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentlatitudelongitudesampling dategeographic locationisolation date
4980patient suffering from cystic fibrosisBelgiumBELEurope50.50114.4765
51924Human sputum,cystic fibrosisBelgiumBELEurope1992Brussels
121175Cystic fibrosis patientBelgiumBELEurope1992

isolation source categories

Cat1Cat2Cat3
#Infection#Disease#Cystic fibrosis
#Infection#Patient

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
49802Risk group (German classification)
1211751Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Burkholderia multivorans 16S ribosomal RNA gene, partial sequenceAF1485551344ena985079
20218Burkholderia multivorans 16S rRNA gene, strain LMG 13010TY187031479ena985079
4980Burkholderia multivorans strain CIP 105495 16S ribosomal RNA gene, partial sequenceEU0241781313ena985079

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Burkholderia multivorans NCTC13007GCA_900446205contigncbi87883
66792Burkholderia multivorans strain NCTC1300787883.262wgspatric87883
66792Burkholderia multivorans NCTC 130072916581165draftimg87883

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileyes89.509no
flagellatedno84.234no
gram-positiveno98.436no
anaerobicno98.871yes
halophileno93.138no
spore-formingno94.169no
thermophileno98.738no
glucose-utilyes90.923no
aerobicyes90.639yes
glucose-fermentno89.199yes

External links

@ref: 4980

culture collection no.: DSM 13243, CIP 105495, LMG 13010, NCTC 13007, CCUG 34080, CCM 4863

straininfo link

  • @ref: 71586
  • straininfo: 7297

literature

  • topic: Metabolism
  • Pubmed-ID: 15096547
  • title: Conservation of the opcL gene encoding the peptidoglycan-associated outer-membrane lipoprotein among representatives of the Burkholderia cepacia complex.
  • authors: Plesa M, Kholti A, Vermis K, Vandamme P, Panagea S, Winstanley C, Cornelis P
  • journal: J Med Microbiol
  • DOI: 10.1099/jmm.0.05504-0
  • year: 2004
  • mesh: Amino Acid Sequence, Bacterial Outer Membrane Proteins/chemistry/*genetics/metabolism, Bacterial Typing Techniques, Burkholderia Infections/*microbiology, Burkholderia cepacia/*classification/*genetics, Cloning, Molecular, *Conserved Sequence, Cystic Fibrosis/*microbiology, Escherichia coli Proteins, Humans, Lipoproteins/chemistry/*genetics/metabolism, Molecular Sequence Data, Peptidoglycan/chemistry/*genetics/metabolism, Phylogeny, Sequence Analysis, DNA, Sputum/microbiology
  • topic2: Phylogeny

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
4980Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 13243)https://www.dsmz.de/collection/catalogue/details/culture/DSM-13243
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
35830Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/17520
51924Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 34080)https://www.ccug.se/strain?id=34080
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68369Automatically annotated from API 20NE
68382Automatically annotated from API zym
71586Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID7297.1StrainInfo: A central database for resolving microbial strain identifiers
121175Curators of the CIPCollection of Institut Pasteur (CIP 105495)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20105495