Strain identifier

BacDive ID: 1912

Type strain: No

Species: Burkholderia gladioli

Strain Designation: B

Strain history: CIP <- 2000, NCIMB <- A. Attafuah, Reading Univ., Reading, UK: strain B

NCBI tax ID(s): 28095 (species)

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General

@ref: 3265

BacDive-ID: 1912

DSM-Number: 8361

keywords: 16S sequence, Bacteria, obligate aerobe, mesophilic, Gram-negative, motile, rod-shaped, plant pathogen

description: Burkholderia gladioli B is an obligate aerobe, mesophilic, Gram-negative plant pathogen that was isolated from surface sterilized macerated mealybug .

NCBI tax id

  • NCBI tax id: 28095
  • Matching level: species

strain history

@refhistory
3265<- NCIMB <- A. Attafuah, strain B
119636CIP <- 2000, NCIMB <- A. Attafuah, Reading Univ., Reading, UK: strain B

doi: 10.13145/bacdive1912.20241212.9.2

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Betaproteobacteria
  • order: Burkholderiales
  • family: Burkholderiaceae
  • genus: Burkholderia
  • species: Burkholderia gladioli
  • full scientific name: Burkholderia gladioli (Severini 1913) Yabuuchi et al. 1993

  • synonyms

    @refsynonym
    20215Burkholderia cocovenenans
    20215Pseudomonas gladioli
    20215Pseudomonas antimicrobica
    20215Pseudomonas cocovenenans

@ref: 3265

domain: Bacteria

phylum: Proteobacteria

class: Betaproteobacteria

order: Burkholderiales

family: Burkholderiaceae

genus: Burkholderia

species: Burkholderia gladioli

full scientific name: Burkholderia gladioli (Severini 1913) Yabuuchi et al. 1993

strain designation: B

type strain: no

Morphology

cell morphology

  • @ref: 119636
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: yes

colony morphology

  • @ref: 3265
  • incubation period: 1-2 days

pigmentation

  • @ref: 119636
  • production: yes
  • name: Pyocyanin

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
3265CASO AGAR (MERCK 105458) (DSMZ Medium 220)yeshttps://mediadive.dsmz.de/medium/220Name: CASO AGAR (Merck 105458) (DSMZ Medium 220) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Distilled water
38636MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
119636CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperature
3265positivegrowth25
38636positivegrowth25
119636positivegrowth25-37
119636nogrowth5
119636nogrowth10
119636nogrowth41

Physiology and metabolism

oxygen tolerance

  • @ref: 119636
  • oxygen tolerance: obligate aerobe

halophily

@refsaltgrowthtested relationconcentration
119636NaClpositivegrowth0-4 %
119636NaClnogrowth6 %
119636NaClnogrowth8 %
119636NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
11963616947citrate+carbon source
1196364853esculin-hydrolysis
11963617632nitrate+reduction
11963616301nitrite-reduction
11963617632nitrate-respiration
6836925115malate+assimilation
6836917128adipate+assimilation
6836927689decanoate+assimilation
6836924265gluconate+assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine+assimilation
6836916899D-mannitol+assimilation
6836916024D-mannose+assimilation
6836930849L-arabinose+assimilation
6836917634D-glucose+assimilation
683695291gelatin+hydrolysis
683694853esculin-hydrolysis
6836916199urea-hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate+reduction

antibiotic resistance

  • @ref: 119636
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

@refChebi-IDmetaboliteproduction
6836935581indoleno
11963635581indoleno

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
3265catalase+1.11.1.6
3265cytochrome-c oxidase-1.9.3.1
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382alkaline phosphatase+3.1.3.1
119636oxidase+
119636beta-galactosidase+3.2.1.23
119636alcohol dehydrogenase-1.1.1.1
119636gelatinase-
119636amylase-
119636DNase-
119636caseinase+3.4.21.50
119636catalase+1.11.1.6
119636tween esterase+
119636lecithinase+
119636lipase-
119636lysine decarboxylase-4.1.1.18
119636ornithine decarboxylase-4.1.1.17
119636protease+
119636tryptophan deaminase-
119636urease-3.5.1.5
68369urease-3.5.1.5
68369arginine dihydrolase-3.5.3.6
68382acid phosphatase+3.1.3.2
68369cytochrome oxidase-1.9.3.1
68369gelatinase+
68369beta-glucosidase-3.2.1.21

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
119636-+++-+----++--------

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
3265+-----+++++++-++++++-
3265+-----+++++++-++++++-
3265+-----+++++++-++++++

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
119636+++++--------+----++++--+-++++-+++--++-+---+++++++++-+--+++++++++--+---+-+-++++++-+-+-+-+++++++++++

Isolation, sampling and environmental information

isolation

@refsample typehost speciescountryorigin.countrycontinent
3265surface sterilized macerated mealybug (Planococcoides njalensis)Planococcoides njalensisGhanaGHAAfrica
119636Surface sterilised macerated mealybugGhanaGHAAfrica

isolation source categories

  • Cat1: #Host
  • Cat2: #Arthropoda
  • Cat3: #Insecta

Safety information

risk assessment

@refpathogenicity plantbiosafety levelbiosafety level comment
3265yes2Risk group (German classification)
1196361Risk group (French classification)

Sequence information

16S sequences

  • @ref: 20218
  • description: Burkholderia gladioli gene for 16S rRNA, strain: NCIMB 9898
  • accession: AB021384
  • length: 1509
  • database: nuccore
  • NCBI tax ID: 28095

External links

@ref: 3265

culture collection no.: DSM 8361, NCIMB 9898, CIP 106719, LMG 18920

straininfo link

  • @ref: 71557
  • straininfo: 12543

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
3265Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 8361)https://www.dsmz.de/collection/catalogue/details/culture/DSM-8361
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
38636Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/18881
68369Automatically annotated from API 20NE
68382Automatically annotated from API zym
71557Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID12543.1StrainInfo: A central database for resolving microbial strain identifiers
119636Curators of the CIPCollection of Institut Pasteur (CIP 106719)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20106719