Strain identifier
BacDive ID: 1909
Type strain: 
Species: Burkholderia cepacia
Strain Designation: B-175
Strain history: <- ICPB <- Quesnel; B-175
NCBI tax ID(s): 292 (species)
version 9.3 (current version)
General
@ref: 12671
BacDive-ID: 1909
DSM-Number: 50180
keywords: genome sequence, Bacteria, mesophilic, Gram-negative, human pathogen
description: Burkholderia cepacia B-175 is a mesophilic, Gram-negative human pathogen that was isolated from Soil.
NCBI tax id
- NCBI tax id: 292
- Matching level: species
strain history
- @ref: 12671
- history: <- ICPB <- Quesnel; B-175
doi: 10.13145/bacdive1909.20250331.9.3
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Betaproteobacteria
- order: Burkholderiales
- family: Burkholderiaceae
- genus: Burkholderia
- species: Burkholderia cepacia
- full scientific name: Burkholderia cepacia (Palleroni and Holmes 1981 ex Burkholder 1950) Yabuuchi et al. 1993
synonyms
- @ref: 20215
- synonym: Pseudomonas cepacia
@ref: 12671
domain: Bacteria
phylum: Proteobacteria
class: Betaproteobacteria
order: Burkholderiales
family: Burkholderiaceae
genus: Burkholderia
species: Burkholderia cepacia
full scientific name: Burkholderia cepacia (Palleroni and Holmes 1981) Yabuuchi et al. 1993
strain designation: B-175
type strain: no
Morphology
cell morphology
| @ref | gram stain | confidence |
|---|---|---|
| 125439 | negative | 98.4 |
| 125438 | negative | 98.5 |
colony morphology
- @ref: 12671
- incubation period: 1-2 days
Culture and growth conditions
culture medium
| @ref | name | growth | link | composition |
|---|---|---|---|---|
| 12671 | NUTRIENT AGAR (DSMZ Medium 1) | yes | https://mediadive.dsmz.de/medium/1 | Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water |
| 12671 | TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) | yes | https://mediadive.dsmz.de/medium/92 | Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water |
| 12671 | COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) | yes | https://mediadive.dsmz.de/medium/693 | Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base |
culture temp
- @ref: 12671
- growth: positive
- type: growth
- temperature: 30
Physiology and metabolism
oxygen tolerance
- @ref: 125439
- oxygen tolerance: obligate aerobe
- confidence: 98.5
spore formation
- @ref: 125439
- spore formation: no
- confidence: 97.8
compound production
| @ref | compound |
|---|---|
| 12671 | purple phenazine pigment |
| 12671 | lipase |
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 68369 | 25115 | malate | + | assimilation |
| 68369 | 17128 | adipate | + | assimilation |
| 68369 | 27689 | decanoate | + | assimilation |
| 68369 | 24265 | gluconate | + | assimilation |
| 68369 | 17306 | maltose | + | assimilation |
| 68369 | 59640 | N-acetylglucosamine | + | assimilation |
| 68369 | 16024 | D-mannose | + | assimilation |
| 68369 | 30849 | L-arabinose | + | assimilation |
| 68369 | 17634 | D-glucose | + | assimilation |
| 68369 | 5291 | gelatin | + | hydrolysis |
| 68369 | 4853 | esculin | + | hydrolysis |
| 68369 | 16199 | urea | - | hydrolysis |
| 68369 | 29016 | arginine | - | hydrolysis |
| 68369 | 27897 | tryptophan | - | energy source |
| 68369 | 17632 | nitrate | - | reduction |
metabolite production
- @ref: 68369
- Chebi-ID: 35581
- metabolite: indole
- production: no
metabolite tests
- @ref: 68369
- Chebi-ID: 35581
- metabolite: indole
- indole test: -
enzymes
| @ref | value | activity | ec |
|---|---|---|---|
| 12671 | catalase | + | 1.11.1.6 |
| 12671 | cytochrome-c oxidase | + | 1.9.3.1 |
| 68382 | alpha-fucosidase | - | 3.2.1.51 |
| 68382 | alpha-mannosidase | - | 3.2.1.24 |
| 68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
| 68382 | beta-glucosidase | - | 3.2.1.21 |
| 68382 | beta-glucuronidase | - | 3.2.1.31 |
| 68382 | alpha-glucosidase | - | 3.2.1.20 |
| 68382 | alpha-galactosidase | - | 3.2.1.22 |
| 68382 | beta-galactosidase | - | 3.2.1.23 |
| 68382 | naphthol-AS-BI-phosphohydrolase | + | |
| 68382 | alpha-chymotrypsin | - | 3.4.21.1 |
| 68382 | trypsin | - | 3.4.21.4 |
| 68382 | valine arylamidase | - | |
| 68382 | cystine arylamidase | - | 3.4.11.3 |
| 68382 | leucine arylamidase | - | 3.4.11.1 |
| 68382 | lipase (C 14) | + | |
| 68382 | esterase (C 4) | - | |
| 68382 | esterase lipase (C 8) | + | |
| 68382 | alkaline phosphatase | + | 3.1.3.1 |
| 68382 | acid phosphatase | + | 3.1.3.2 |
| 68369 | cytochrome oxidase | + | 1.9.3.1 |
| 68369 | gelatinase | + | |
| 68369 | beta-glucosidase | + | 3.2.1.21 |
| 68369 | urease | - | 3.5.1.5 |
| 68369 | arginine dihydrolase | - | 3.5.3.6 |
API zym
| @ref | Control | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 44593 | - | + | - | + | + | - | - | - | - | - | + | + | - | - | - | - | - | - | - | - |
API 20NE
| @ref | NO3 | TRP | GLU_ Ferm | ADH Arg | URE | ESC | GEL | PNPG | GLU_ Assim | ARA | MNE | MAN | NAG | MAL | GNT | CAP | ADI | MLT | CIT | PAC | OX |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 12671 | - | - | - | - | - | + | + | + | + | + | + | +/- | + | + | + | + | + | + | + | + | + |
| 12671 | - | - | - | - | - | + | + | + | + | + | + | +/- | + | + | + | + | + | + | + | + | + |
| 12671 | - | - | + | - | - | + | + | + | + | + | + | +/- | + | + | + | + | + | + | + | + | |
| 12671 | - | - | - | - | - | + | + | + | + | + | + | - | + | + | + | + | + | + | + | + | + |
| 44593 | - | - | - | - | - | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + |
Isolation, sampling and environmental information
isolation
- @ref: 44593
- sample type: Soil
- geographic location: Trinidad
- country: Trinidad and Tobago
- origin.country: TTO
- continent: Middle and South America
isolation source categories
- Cat1: #Environmental
- Cat2: #Terrestrial
- Cat3: #Soil
Safety information
risk assessment
- @ref: 12671
- pathogenicity human: yes
- pathogenicity animal: yes
- pathogenicity plant: yes
- biosafety level: 2
- biosafety level comment: Risk group (German classification)
Sequence information
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Burkholderia lata LMG 6993 | GCA_902499145 | contig | ncbi | 482957 |
| 66792 | Burkholderia lata strain LMG 6993 | 482957.58 | wgs | patric | 482957 |
Genome-based predictions
predictions
| @ref | model | trait | description | prediction | confidence | training_data |
|---|---|---|---|---|---|---|
| 125438 | gram-positive | gram-positive | Positive reaction to Gram-staining | no | 98.5 | no |
| 125438 | anaerobic | anaerobic | Ability to grow under anoxygenic conditions (including facultative anaerobes) | no | 98.322 | no |
| 125438 | spore-forming | spore-forming | Ability to form endo- or exospores | no | 87.561 | no |
| 125438 | aerobic | aerobic | Ability to grow under oxygenic conditions (including facultative aerobes) | yes | 89.236 | no |
| 125438 | thermophile | thermophilic | Ability to grow at temperatures above or equal to 45°C | no | 98 | yes |
| 125438 | motile2+ | flagellated | Ability to perform flagellated movement | yes | 84.256 | no |
| 125439 | BacteriaNet | spore_formation | Ability to form endo- or exospores | no | 97.8 | |
| 125439 | BacteriaNet | motility | Ability to perform movement | yes | 83.6 | |
| 125439 | BacteriaNet | gram_stain | Reaction to gram-staining | negative | 98.4 | |
| 125439 | BacteriaNet | oxygen_tolerance | Oxygenic conditions needed for growth | obligate aerobe | 98.5 |
External links
@ref: 12671
culture collection no.: DSM 50180, ATCC 17770, NCIB 9089, CCUG 2858, LMG 6993
straininfo link
- @ref: 71554
- straininfo: 3974
Reference
| @id | authors | catalogue | doi/url | title |
|---|---|---|---|---|
| 12671 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 50180) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-50180 | |
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
| 44593 | Curators of the CCUG | Culture Collection University of Gothenburg (CCUG) (CCUG 2858) | https://www.ccug.se/strain?id=2858 | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |
| 68369 | Automatically annotated from API 20NE | |||
| 68382 | Automatically annotated from API zym | |||
| 71554 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID3974.1 | StrainInfo: A central database for resolving microbial strain identifiers | |
| 125438 | Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann | 10.1101/2024.08.12.607695 | Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets | |
| 125439 | Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy | https://github.com/GenomeNet/deepG | deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 |