Strain identifier
BacDive ID: 1903
Type strain:
Species: Pseudochrobactrum lubricantis
Strain history: <- CCUG <- P. Kämpfer, Univ. Giessen, Germany <- K. Nehrenheim, Germany
NCBI tax ID(s): 558172 (species)
General
@ref: 17372
BacDive-ID: 1903
DSM-Number: 23782
keywords: 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, rod-shaped
description: Pseudochrobactrum lubricantis DSM 23782 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from water mixed metal working fluid.
NCBI tax id
- NCBI tax id: 558172
- Matching level: species
strain history
- @ref: 17372
- history: <- CCUG <- P. Kämpfer, Univ. Giessen, Germany <- K. Nehrenheim, Germany
doi: 10.13145/bacdive1903.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Alphaproteobacteria
- order: Hyphomicrobiales
- family: Brucellaceae
- genus: Pseudochrobactrum
- species: Pseudochrobactrum lubricantis
- full scientific name: Pseudochrobactrum lubricantis Kämpfer et al. 2009
@ref: 17372
domain: Bacteria
phylum: Proteobacteria
class: Alphaproteobacteria
order: Rhizobiales
family: Brucellaceae
genus: Pseudochrobactrum
species: Pseudochrobactrum lubricantis
full scientific name: Pseudochrobactrum lubricantis Kämpfer et al. 2009
type strain: yes
Morphology
cell morphology
- @ref: 23348
- gram stain: negative
- cell shape: rod-shaped
- motility: no
colony morphology
@ref | type of hemolysis | colony size | colony color | colony shape | incubation period |
---|---|---|---|---|---|
17372 | gamma | ||||
23348 | 2 mm | beige, translucent | circular | 1 day | |
61143 | 1 day |
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
17372 | R2A MEDIUM (DSMZ Medium 830) | yes | https://mediadive.dsmz.de/medium/830 | Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water |
23348 | MacConkey | yes | ||
23348 | Nutrient agar (NA) | yes | ||
23348 | Reasoner's 2A agar (R2A) | yes | ||
23348 | Tryptone soy agar | yes |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
17372 | positive | growth | 30 | mesophilic |
61143 | positive | growth | 30 | mesophilic |
Physiology and metabolism
oxygen tolerance
- @ref: 61143
- oxygen tolerance: aerobe
spore formation
- @ref: 23348
- spore formation: no
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
23348 | 65327 | D-xylose | - | assimilation |
23348 | 37054 | 3-hydroxybutyrate | - | assimilation |
23348 | 30849 | L-arabinose | - | assimilation |
23348 | 62345 | L-rhamnose | - | assimilation |
23348 | 16865 | gamma-aminobutyric acid | + | assimilation |
23348 | 16958 | beta-alanine | + | assimilation |
23348 | 15824 | D-fructose | + | assimilation |
23348 | 12936 | D-galactose | + | assimilation |
23348 | 17634 | D-glucose | + | assimilation |
23348 | 16024 | D-mannose | + | assimilation |
23348 | 16988 | D-ribose | + | assimilation |
23348 | 24996 | lactate | + | assimilation |
23348 | 29806 | fumarate | + | assimilation |
23348 | 17859 | glutaric acid | + | assimilation |
23348 | 29991 | L-aspartate | + | assimilation |
23348 | 15971 | L-histidine | + | assimilation |
23348 | 15589 | L-malate | + | assimilation |
23348 | 506227 | N-acetylglucosamine | + | assimilation |
23348 | 18257 | ornithine | + | assimilation |
23348 | 17272 | propionate | + | assimilation |
23348 | 15361 | pyruvate | + | assimilation |
23348 | 16383 | cis-aconitate | +/- | assimilation |
68369 | 25115 | malate | + | assimilation |
68369 | 17128 | adipate | - | assimilation |
68369 | 27689 | decanoate | - | assimilation |
68369 | 24265 | gluconate | - | assimilation |
68369 | 17306 | maltose | - | assimilation |
68369 | 59640 | N-acetylglucosamine | + | assimilation |
68369 | 16899 | D-mannitol | - | assimilation |
68369 | 16024 | D-mannose | + | assimilation |
68369 | 30849 | L-arabinose | - | assimilation |
68369 | 17634 | D-glucose | + | assimilation |
68369 | 5291 | gelatin | - | hydrolysis |
68369 | 4853 | esculin | - | hydrolysis |
68369 | 16199 | urea | + | hydrolysis |
68369 | 29016 | arginine | - | hydrolysis |
68369 | 17634 | D-glucose | - | fermentation |
68369 | 27897 | tryptophan | - | energy source |
68369 | 17632 | nitrate | - | reduction |
metabolite production
- @ref: 68369
- Chebi-ID: 35581
- metabolite: indole
- production: no
metabolite tests
- @ref: 68369
- Chebi-ID: 35581
- metabolite: indole
- indole test: -
enzymes
@ref | value | activity | ec |
---|---|---|---|
17372 | catalase | + | 1.11.1.6 |
17372 | cytochrome-c oxidase | + | 1.9.3.1 |
23348 | cytochrome oxidase | + | 1.9.3.1 |
68369 | cytochrome oxidase | + | 1.9.3.1 |
68369 | gelatinase | - | |
68369 | beta-glucosidase | - | 3.2.1.21 |
68369 | urease | + | 3.5.1.5 |
68369 | arginine dihydrolase | - | 3.5.3.6 |
API 20NE
@ref | NO3 | TRP | GLU_ Ferm | ADH Arg | URE | ESC | GEL | PNPG | GLU_ Assim | ARA | MNE | MAN | NAG | MAL | GNT | CAP | ADI | MLT | CIT | PAC | OX |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
17372 | - | - | - | - | + | - | - | - | + | - | + | - | + | - | - | - | - | + | - | - | + |
Isolation, sampling and environmental information
isolation
@ref | sample type | country | origin.country | continent | enrichment culture | sampling date |
---|---|---|---|---|---|---|
17372 | water mixed metal working fluid | Germany | DEU | Europe | ||
23348 | tryptone soy agar | |||||
61143 | Water mixed metal working fluid | Germany | DEU | Europe | 2000 |
isolation source categories
Cat1 | Cat2 |
---|---|
#Engineered | #Contamination |
#Environmental | #Aquatic |
taxonmaps
- @ref: 69479
- File name: preview.99_3029.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_5;96_1591;97_1899;98_2331;99_3029&stattab=map
- Last taxonomy: Pseudochrobactrum
- 16S sequence: FM209496
- Sequence Identity:
- Total samples: 1773
- soil counts: 281
- aquatic counts: 437
- animal counts: 988
- plant counts: 67
Safety information
risk assessment
- @ref: 17372
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 17372
- description: Pseudochrobactrum sp. KSS 7.8 partial 16S rRNA gene, type strain KSS 7.8T
- accession: FM209496
- length: 1401
- database: ena
- NCBI tax ID: 558172
External links
@ref: 17372
culture collection no.: DSM 23782, CCM 7581, CCUG 56963, KSS 7.8
straininfo link
- @ref: 71547
- straininfo: 396774
literature
- topic: Phylogeny
- Pubmed-ID: 19622660
- title: Pseudochrobactrum lubricantis sp. nov., isolated from a metal-working fluid.
- authors: Kampfer P, Huber B, Lodders N, Warfolomeow I, Busse HJ, Scholz HC
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijs.0.008540-0
- year: 2009
- mesh: Bacterial Typing Techniques, Brucellaceae/classification/genetics/*isolation & purification/metabolism, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fatty Acids/chemistry/metabolism, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, *Water Microbiology
- topic2: Metabolism
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
17372 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 23782) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-23782 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
23348 | Peter Kämpfer, Birgit Huber, Nicole Lodders, Isabel Warfolomeow, Hans-Jürgen Busse, Holger C. Scholz | 10.1099/ijs.0.008540-0 | Pseudochrobactrum lubricantis sp. nov., isolated from a metal-working fluid | IJSEM 59: 2464-2467 2009 | 19622660 | |
61143 | Curators of the CCUG | Culture Collection University of Gothenburg (CCUG) (CCUG 56963) | https://www.ccug.se/strain?id=56963 | |||
68369 | Automatically annotated from API 20NE | |||||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
71547 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID396774.1 | StrainInfo: A central database for resolving microbial strain identifiers |