Strain identifier
BacDive ID: 19
Type strain:
Species: Acetobacter oeni
Strain Designation: B13
Variant: Isotype of BacDive ID 132838
Strain history: CIP <- 2006, CECT <- 2003, E. Velazquez, Viseu, Portugal: strain B13
NCBI tax ID(s): 1307912 (strain), 304077 (species)
General
@ref: 17309
BacDive-ID: 19
DSM-Number: 23926
keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, mesophilic, Gram-negative, motile, rod-shaped, colony-forming
description: Acetobacter oeni B13 is an obligate aerobe, mesophilic, Gram-negative bacterium that forms circular colonies and was isolated from spoiled red wine.
NCBI tax id
NCBI tax id | Matching level |
---|---|
1307912 | strain |
304077 | species |
strain history
@ref | history |
---|---|
17309 | <- CECT <- E. Velázquez <- L. R. Silva, Depart. Microbiol. y Gen., Univ. Salamanca, Spain |
121399 | CIP <- 2006, CECT <- 2003, E. Velazquez, Viseu, Portugal: strain B13 |
doi: 10.13145/bacdive19.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Alphaproteobacteria
- order: Rhodospirillales
- family: Acetobacteraceae
- genus: Acetobacter
- species: Acetobacter oeni
- full scientific name: Acetobacter oeni Silva et al. 2006
@ref: 17309
domain: Bacteria
phylum: Proteobacteria
class: Alphaproteobacteria
order: Rhizobiales
family: Acetobacteraceae
genus: Acetobacter
species: Acetobacter oeni
full scientific name: Acetobacter oeni Silva et al. 2006
strain designation: B13
variant: Isotype of BacDive ID 132838
type strain: yes
Morphology
cell morphology
@ref | gram stain | cell length | cell width | cell shape | motility | flagellum arrangement | confidence |
---|---|---|---|---|---|---|---|
23234 | negative | 3.6-5.1 µm | 0.8-0.9 µm | rod-shaped | yes | peritrichous | |
69480 | negative | 99.994 | |||||
121399 | negative | rod-shaped | yes |
colony morphology
- @ref: 23234
- colony size: 1-2 mm
- colony color: cream, opaque
- colony shape: circular
- incubation period: 4 days
- medium used: YEDC
multimedia
@ref | multimedia content | caption | intellectual property rights |
---|---|---|---|
17309 | https://www.dsmz.de/microorganisms/photos/DSM_23926.jpg | Micrograph of DSM 23626. Scale bar represents 2 um. | Leibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH |
17309 | https://www.dsmz.de/microorganisms/photos/DSM_23926-1.jpg | DSM 23926 grown on DSMZ meidum # 360 at 25°C. | Leibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH |
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
17309 | YPM MEDIUM (DSMZ Medium 360) | yes | https://mediadive.dsmz.de/medium/360 | Name: YPM MEDIUM (DSMZ Medium 360) Composition: Mannitol 25.0 g/l Agar 12.0 g/l Yeast extract 5.0 g/l Peptone 3.0 g/l Distilled water |
23234 | YEDC | yes | 7 g glucose, 3 g yeast extract, 20 g calcium carbonate and 17 g agar | |
37461 | MEDIUM 72- for trypto casein soja agar | yes | Distilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g) | |
121399 | CIP Medium 314 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=314 | |
121399 | CIP Medium 72 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
17309 | positive | growth | 25 | mesophilic |
23234 | positive | optimum | 28 | mesophilic |
37461 | positive | growth | 25 | mesophilic |
Physiology and metabolism
tolerance
- @ref: 23234
- compound: Ethanol
- percentage: 10
oxygen tolerance
@ref | oxygen tolerance |
---|---|
23234 | obligate aerobe |
121399 | obligate aerobe |
spore formation
@ref | spore formation | confidence |
---|---|---|
23234 | no | |
69481 | no | 100 |
69480 | no | 99.999 |
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
23234 | 17306 | maltose | - | carbon source |
23234 | 17790 | methanol | - | carbon source |
23234 | 17754 | glycerol | + | carbon source |
121399 | 17632 | nitrate | - | reduction |
121399 | 16301 | nitrite | - | reduction |
metabolite production
@ref | Chebi-ID | metabolite | production |
---|---|---|---|
23234 | 16808 | 2-dehydro-D-gluconate | no |
23234 | 17426 | 5-dehydro-D-gluconate | yes |
121399 | 35581 | indole | no |
enzymes
@ref | value | activity | ec |
---|---|---|---|
68382 | alpha-fucosidase | - | 3.2.1.51 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | N-acetyl-beta-glucosaminidase | + | 3.2.1.52 |
68382 | beta-glucosidase | + | 3.2.1.21 |
68382 | alpha-glucosidase | - | 3.2.1.20 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | beta-galactosidase | - | 3.2.1.23 |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | naphthol-AS-BI-phosphohydrolase | + | |
68382 | acid phosphatase | - | 3.1.3.2 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | trypsin | - | 3.4.21.4 |
68382 | cystine arylamidase | - | 3.4.11.3 |
68382 | valine arylamidase | - | |
68382 | leucine arylamidase | + | 3.4.11.1 |
68382 | lipase (C 14) | - | |
68382 | esterase lipase (C 8) | + | |
68382 | esterase (C 4) | + | |
68382 | alkaline phosphatase | - | 3.1.3.1 |
121399 | oxidase | - | |
121399 | catalase | + | 1.11.1.6 |
121399 | urease | - | 3.5.1.5 |
API zym
@ref | Control | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
121399 | - | - | + | + | - | + | - | - | - | - | - | + | - | - | - | - | + | + | - | - |
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent |
---|---|---|---|---|---|
17309 | spoiled red wine | Dão region, Viseu | Portugal | PRT | Europe |
121399 | Food, Red wine | Dao | Portugal | PRT | Europe |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Engineered | #Food production | #Fermented |
#Environmental | #Microbial community | |
#Engineered | #Food production | #Beverage |
Safety information
risk assessment
@ref | biosafety level | biosafety level comment |
---|---|---|
17309 | 1 | Risk group (German classification) |
121399 | 1 | Risk group (French classification) |
Sequence information
16S sequences
@ref | description | accession | length | database | NCBI tax ID |
---|---|---|---|---|---|
20218 | Acetobacter oeni strain CECT 5830 16S ribosomal RNA gene, partial sequence | HQ398213 | 990 | ena | 304077 |
20218 | Acetobacter oeni strain LMG 21952 16S ribosomal RNA gene, partial sequence | JF793961 | 1351 | ena | 304077 |
17309 | Acetobacter oeni strain B13 16S ribosomal RNA gene, complete sequence | AY829472 | 1484 | ena | 304077 |
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Acetobacter oeni LMG 21952 | GCA_011516825 | contig | ncbi | 304077 |
66792 | Acetobacter oeni DSM 23926 | GCA_014196315 | contig | ncbi | 304077 |
66792 | Acetobacter oeni strain DSM 23926 | 304077.9 | wgs | patric | 304077 |
66792 | Acetobacter oeni strain LMG 21952 | 304077.8 | wgs | patric | 304077 |
66792 | Acetobacter oeni DSM 23926 | 2829864053 | draft | img | 304077 |
GC content
@ref | GC-content | method |
---|---|---|
17309 | 58.1 | high performance liquid chromatography (HPLC) |
23234 | 58.10 | high performance liquid chromatography (HPLC) |
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
spore-forming | no | 100 | no |
gram-positive | no | 97.163 | yes |
anaerobic | no | 97.838 | yes |
halophile | no | 93.976 | no |
spore-forming | no | 94.983 | yes |
glucose-util | yes | 89.606 | no |
flagellated | no | 66.22 | yes |
aerobic | yes | 92.001 | yes |
motile | yes | 85.308 | yes |
thermophile | no | 96.7 | no |
glucose-ferment | no | 91.049 | no |
External links
@ref: 17309
culture collection no.: DSM 23926, CECT 5830, LMG 21952, CIP 109171
straininfo link
- @ref: 69703
- straininfo: 100264
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 16403860 | Acetobacter oeni sp. nov., isolated from spoiled red wine. | Silva LR, Cleenwerck I, Rivas R, Swings J, Trujillo ME, Willems A, Velazquez E | Int J Syst Evol Microbiol | 10.1099/ijs.0.46000-0 | 2006 | Acetobacter/*classification/genetics/growth & development/isolation & purification/metabolism, Base Composition, Catalase/metabolism, Culture Media, DNA, Bacterial, *Food Microbiology, Gluconates/metabolism, Glucose/metabolism, Glycerol, Molecular Sequence Data, RNA, Bacterial/genetics, RNA, Ribosomal, 16S, Sequence Homology, Nucleic Acid, Species Specificity, Wine/*microbiology | Metabolism |
Phylogeny | 26637821 | Acetobacter musti sp. nov., isolated from Bobal grape must. | Ferrer S, Manes-Lazaro R, Benavent-Gil Y, Yepez A, Pardo I | Int J Syst Evol Microbiol | 10.1099/ijsem.0.000818 | 2015 |
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
17309 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 23926) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-23926 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
23234 | Luis R. Silva Ilse Cleenwerck, Raúl Rivas, Jean Swings, Martha E. Trujillo, Anne Willems, Encarna Velázquez | 10.1099/ijs.0.46000-0 | Acetobacter oeni sp. nov., isolated from spoiled red wine | IJSEM 56: 21-24 2006 | 16403860 | |
37461 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/6882 | ||||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
66794 | Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg | 10.1093/nar/gkaa1025 | BRENDA, the ELIXIR core data resource in 2021: new developments and updates | Nucleic Acids Res. 49: D498-D508 2020 | 33211880 | |
68382 | Automatically annotated from API zym | |||||
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |||
69703 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID100264.1 | StrainInfo: A central database for resolving microbial strain identifiers | |||
121399 | Curators of the CIP | Collection of Institut Pasteur (CIP 109171) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20109171 |